The Science of Stress
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Big Secrets in Small Genes the Brain, Inflammation, Cancer, And
NATIONAL INSTITUTES OF HEALTH • OFFICE OF THE DIRECTOR | VOLUME 25 ISSUE 6 • NOVEMBER-DECEMBER 2017 Big Secrets in Small The Brain, Inflammation, Cancer, and More Genes Report from the 2017 NIH Research Festival The Work of Gisela Storz, Ph.D. BY JENNIFER PATTERSON-WEST, NIDDK Her colleagues laughed at her “crazy idea” when she was a graduate student at the University of California, Berkeley, in the 1980s. Gisela Storz had predicted that a single protein (OxyR) could sense a destructive oxidant, hydro- gen peroxide, bind to DNA, and turn on genes that would neutralize the threat. But Storz has gotten the last laugh. Turns D. NEMECEK, J.B. HEYMANN, A.C. STEVEN, NIAMS out that her hypothesis was correct. Storz, now a distinguished investigator at the National Institute of Child Health and Human Development and a member of the National Academy of Sciences, continued to study OxyR throughout her training and later as an investigator at NIH. For many years, her lab studied redox-sensitive transcription factors and the bacterial and yeast responses to oxidative stress. Her group discovered that the activity of the Escherichia coli transcription factor You guessed right: The bacteriophage Phi-6, which serves as a model system for rotavirus, the most common cause of diarrheal disease among infants and young children. Shown here, a cryo-electron microscopy image of Phi-6‘s protein shell, OxyR is regulated by a reversible disulfide- or procapsid, cut open to show the different types of protein: P1 (blue), P4 (red), P7, yellow, and P2 (purple). bond formation (Science 279:1718–1721, 1998). -
Dsra RNA Regulates Translation of Rpos Message by an Anti-Antisense Mechanism, Independent of Its Action As an Antisilencer of Transcription
Proc. Natl. Acad. Sci. USA Vol. 95, pp. 12462–12467, October 1998 Genetics DsrA RNA regulates translation of RpoS message by an anti-antisense mechanism, independent of its action as an antisilencer of transcription NADIM MAJDALANI†,CHRISTOFER CUNNING‡,DARREN SLEDJESKI§,TOM ELLIOTT‡, AND SUSAN GOTTESMAN†¶ †Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892; §Department of Microbiology and Immunology, Medical College of Ohio, Toledo, OH 43614; and ‡Department of Microbiology and Immunology, West Virginia University Health Sciences Center, Morgantown, WV 26506 Contributed by Susan Gottesman, August 19, 1998 ABSTRACT DsrA RNA regulates both transcription, by MATERIALS AND METHODS overcoming transcriptional silencing by the nucleoid- Bacterial Strains. Unless otherwise noted, strains were associated H-NS protein, and translation, by promoting effi- 1 D cient translation of the stress s factor, RpoS. These two derivatives of either NM22180 (dsrA ) or NM22181 ( dsrA). activities of DsrA can be separated by mutation: the first of These isogenic strains, derived by P1 transduction from three stem-loops of the 85 nucleotide RNA is necessary for SG20250 (3), carry deletions of the lac operon and of the ara RpoS translation but not for anti-H-NS action, while the region, introduced from LMG194 (4). The dsrA deletion was introduced by P1 transduction from strain DDS719, selecting second stem-loop is essential for antisilencing and less critical R for RpoS translation. The third stem-loop, which behaves as for the linked Tet marker and screened by PCR as described a transcription terminator, can be substituted by the trp previously (2). The various rpoS-lac fusions were constructed transcription terminator without loss of either DsrA function. -
Curriculum Vitae
Robin W. Morgan, Ph.D. Professor, Department of Animal and Food Sciences Delaware Biotechnology Institute 15 Innovation Way University of Delaware Newark, DE 19716-2103 (302) 383-4806 (phone) Email: [email protected] EDUCATION • Meredith College, Raleigh, North Carolina, B.S., Biology, 1977 • The Johns Hopkins University, Baltimore, Maryland, Ph.D., Biology, 1982 • University of California, Berkeley, California, Post-doctoral fellow, Biochemistry, 1982-1985 PROFESSIONAL EXPERIENCE • Dean and Director of the Agricultural Experiment Station, College of Agriculture and Natural Resources, University of Delaware, July 2002-July 2012 • Acting Dean, Director of the Agricultural Experiment Station, and Director of Cooperative Extension, College of Agriculture and Natural Resources, University of Delaware, July 2001-June 2002 • Associate Dean for Research and Associate Director of the Agricultural Experiment Station, College of Agriculture and Natural Resources, University of Delaware, 2000- 2001 • Professor, Department of Animal and Food Sciences, University of Delaware, Newark, DE; 1996-present • Joint appointment in the Department of Chemistry and Biochemistry, University of Delaware, 1993-present • Joint appointment in the Department of Biology, University of Delaware, 1990- present • Assistant Department Chairperson, Department of Animal and Food Sciences, University of Delaware, Newark, DE, 1992-2000 • Associate Professor, Department of Animal and Food Sciences, University of Delaware, Newark, DE, 1991-1996 • Assistant Professor, Department -
Are You Suprised ?
F.M. Ausubel -- CV -- December 14, 2005 -- page - 1 FREDERICK MICHAEL AUSUBEL CURRICULUM VITAE BIRTH DATE: September 2, 1945 CITIZENSHIP: U.S.A. CONTACT INFORMATION: Department of Molecular Biology Richard B. Simches Research Building 185 Cambridge Street Massachusetts General Hospital Boston, MA 02114 Phone: 617-726-5969 FAX: 617-726-5949 Email: [email protected] PRESENT POSITIONS: Professor of Genetics, Department of Genetics, Harvard Medical School. Molecular Biologist, Department of Molecular Biology, Massachusetts General Hospital. EDUCATION: 1. Massachusetts Institute of Technology, Cambridge, Massachusetts. Ph.D. in Biology, 1972. 2. University of Illinois, Urbana, Illinois. B.S. in Chemistry, 1966. RESEARCH EXPERIENCE: 1. September 1, 1982 to present: Professor of Genetics, Harvard Medical School, Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts. Molecular genetics of nitrogen fixation genes; molecular genetics of Arabidopsis thaliana; molecular genetics of host-pathogen interactions. 2. September 1, 1975 to August 31, 1982: Assistant and Associate Professor of Biology, Department of Cellular and Developmental Biology, Harvard University. Molecular genetics of nitrogen fixation genes in Klebsiella pneumoniae and Rhizobium meliloti. 3. January 1, 1974 to September 1, 1975: Postdoctoral Research Fellow, Harvard University and Universities of Leicester and Sussex. Molecular genetics of nitrogen fixation genes; Somatic cell genetics of Arabidopsis thaliana. 4. 1972 and 1973: Instructor and Research Associate, M.I.T. Molecular genetic analysis of nitrogen fixation genes in Klebsiella pneumoniae. 5. 1966 to 1971: Graduate Student, M.I.T. Purification and properties of bacteriophage lambda integrase. Thesis supervisor: Dr. Ethan Signer. 6. 1964 to 1965: Undergraduate Senior Thesis, University of Illinois. Purification and properties of borneol dehydrogenase.