Pantospora Chromolaenae Fungal Planet Description Sheets 351

350 Persoonia – Volume 42, 2019

Pantospora chromolaenae Fungal Planet description sheets 351

Fungal Planet 890 – 19 July 2019 Pantospora chromolaenae Crous & Cheew., sp. nov. Etymology. Name refers to Chromolaena, the host genus from which this Notes — Pantospora is characterised by conidiogenous fungus was isolated. cells with sympodial and percurrent proliferation, and pseudo Classiﬁcation — Mycosphaerellaceae, Capnodiales, Dothi cercospora-like conidia that have transverse, and often also deomycetes. oblique to longitudinal septa (Minnis et al. 2011, Videira et al. 2017). Pantospora chromolaenae represents a new species Mycelium consisting of pale brown, smooth-walled, septate, on Chromolaena odorata in Thailand. branched, 2.5–3 mm diam hyphae. Conidiophores solitary, Based on a megablast search of NCBIs GenBank nucleotide erect, straight to flexuous, subcylindrical, 1–6-septate, 20–70 database, the closest hits using the ITS sequence had high- × 3–6 mm, medium brown, smooth to verruculose, mostly est similarity to Rhachisphaerella mozambica (GenBank unbranched. Conidiogenous cells medium brown, subcylindri- MH863208.1; Identities = 506/514 (98 %), 3 gaps (0 %)), cal, smooth to verruculose, 10–15 × 3–6 mm, terminal and Pantospora guazumae (GenBank NR_119971.1; Identities = intercalary, scars thickened, darkened, refractive, 2–3 mm 506/514 (98 %), 3 gaps (0 %)) and Amycosphaerella afri diam. Conidia solitary, unbranched, obclavate, straight to cana (as Mycosphaerella aurantia, GenBank EU853468.1; flexuous, medium brown, verruculose, granular, apex obtuse, Identities = 506/514 (98 %), 3 gaps (0 %)). Closest hits base truncate, 2–2.5 mm diam, thickened, darkened, refrac- using the LSU sequence are Ragnhildiana diffusa (GenBank tive, (3–)6–8(–12) transversely septate, conidia becoming MH866148.1; Identities = 831/833 (99 %), 1 gap (0 %)), Rag muriformly septate, starting with basal cells, (24–)50–65(–80) nhildiana pseudotithoniae (GenBank NG_058049.1; Identi- × (4–)5–6(–7) mm. ties = 831/833 (99 %), 1 gap (0 %)) and Ragnhildiana perfoliati Culture characteristics — Colonies erumpent, spreading, (GenBank GU214453.1; Identities = 815/817 (99 %), 1 gap surface folded, with moderate aerial mycelium and smooth, (0 %)). Closest hits using the actA sequence had highest lobate margin, reaching 25 mm diam after 2 wk at 25 °C. On similarity to Amycosphaerella africana (GenBank KF903407.1; MEA, PDA and OA surface olivaceous grey, reverse iron-grey. Identities = 496/520 (95 %), 5 gaps (0 %)), Rhachisphae Typus. Thailand, Songkhla, Hat Yai, on leaves of Chromolaena odorata rella mozambica (as Mycosphaerella mozambica, GenBank (Asteraceae), 2008, R. Cheewangkoon (holotype CBS H-23957, culture EU514319.1; Identities = 504/531 (95 %), 4 gaps (0 %)) and ex-type MC14 = CPC 34870 = CBS 145563, ITS, LSU, actA, his3 and rpb2 Camptomeriphila leucaenae (GenBank KY173563.1; Identities sequences GenBank MK876401.1, MK876442.1, MK876459.1, MK876476.1 = 446/474 (94 %), 5 gaps (1 %)). No actA sequence of Panto and MK876488.1, MycoBank MB830848). spora was available for comparison. Closest hits using the his3 sequence had highest similarity to Rhachisphaerella mozambica (as Mycosphaerella mozambica, GenBank EU514371.1; Identi- ties = 371/382 (97 %), 2 gaps (0 %)), Pseudocercosporella bakeri (GenBank KX288752.1; Identities = 353/371 (95 %), 3 gaps (0 %)) and Pseudocercospora indonesiana (Gen- Bank EU514393.1; Identities = 356/390 (91 %), 7 gaps (1 %)). No his3 sequence of Pantospora was available for comparison. Closest hits using the rpb2 sequence had highest similarity to Amycosphaerella africana (GenBank MF951432.1; Identi- ties = 765/871 (88 %), no gaps), Asperisporium caricicola (GenBank MF951439.1; Identities = 794/908 (87 %), no gaps) and Asperisporium caricae (GenBank MF951438.1; Identi- ties = 813/930 (87 %), no gaps). The rpb2 sequence is 804/923 (87 %, including 4 gaps) similar to the rpb2 sequence of Panto spora guazumae voucher BPI 880778 (JN190952.1).

Colour illustrations. Temple at Songkhla, Hat Yai. Leaf spots; conidiophores, conidiogenous cells, and muriformly septate conidia. Scale bars = 10 µm.

Pedro W. Crous & Johannes Z. Groenewald, Westerdijk Fungal Biodiversity Institute, P.O. Box 85167, 3508 AD Utrecht, The Netherlands; e-mail: [email protected] & [email protected] Ratchadawan Cheewangkoon, Nisachon Tamakeaw & Sukanya Haitook, Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand; e-mail: [email protected], [email protected] & [email protected]