ISSN 1510-7809
LOTUS NEWSLETTER 2003 Volume 33
Editor: M. Rebuffo INSTITUTO NACIONAL DE INVESTIGACION AGROPECUARIA Editor: M. Rebuffo INSTITUTO NACIONAL DE INVESTIGACION AGROPECUARIA
Editorial Office INIA La Estanzuela Colonia, Uruguay Phone: +598-574-8000 Email: [email protected] This Newsletter consists of informal Fax No.: +598-574-8012 reports which are presented to Web: http://www.inia.org.uy/sitios/lnl/ further the exchange of ideas and information between research workers. Consequently the data presented here are not to be used in Front cover: The picture on the front cover of this volume shows a larva of Bruchophagus publications without the consent of platypterus (Walker) as presented by the authors. R.Alzugaray on pages 11 and 18 of this volume.
The opinions in this publication are those of the authors and not necessarily those of the Lotus Newsletter. The designations employed and the presentation of the material in this publication do not imply the expression of any opinion whatsoever on the part of the Newsletter concerning the legal status of any country, territory, city, or area, or of its autorities, or concerning the delimitation of its frontiers or boundaries. Where trade names are used this does not constitute endorsement of or discrimination against any product by the Newsletter. Lotus Newsletter. (2003). Volume 33.
Contents
Newsletter Announcements and Instructions ii
Personalia iv
Contributions: reports and short communications
W. F. GRANT. The Lotus Newsletter 1970 (No.1) to 1985 (No.15). 1
S. SAREEN and I. DEV. A preliminary study to explore potential of Lotus corniculatus L (Birdsfoot trefoil) in Palampur ( Himachal Pradesh, India). 3
J.F. AYRES. Lotus research update from Eastern Australia. 7
M.S. BARUFALDI, M.C. DE PABLO, R.H. RODRÍGUEZ, M.V. MONTAGNA, R.D. PÉREZ, L. DAULERIO and E. HIDALGO. Differences in pollen fertility and seed quality between diploid and tetraploid cytotypes of Lotus glaber Mill. 9
R.ALZUGARAY. Insect pests damaging Lotus corniculatus L. flowers and seeds in Uruguay. 11
J. CILIUTI, S. ARRIVILLAGA, S. GERMÁN, S. STEWART, M. REBUFFO and S. HERNÁNDEZ. Studies of rust fungi on Lotus subbiflorus and L.uliginosus. 19
Lotus activities: Background and present research 25
Current list of Lotus researchers. Database last updated Nov. 30 2003 37
Lotus literature. 63
Lotus Newsletter. 2003. Volume 33.
Newsletter Announcements and Instructions
The Lotus Newsletter has been published annually since 1971 by Dr. W.Grant, Dr. R.McGraw and Dr. P.Beuselinck, and it will be published by INIA, Uruguay in the future. It is intended as a worldwide communication link for all those who are interested in the research and development of Lotus species. Persons interested in lotus improvement, genetics, molecular biology, microbiology, production, marketing, or utilization are invited to contribute to the Lotus Newsletter. Previous issues may be used as a guide. It is expected that the work reported will be developed further and formally published later in refereed journals. It is assumed that contributions in LN will not be cited unless no alternative reference is available.
Acknowledgement
INIA is pleased to host Lotus Newsletter. We would like to express our gratefulness to Dr. Paul Beuselinck for providing Uruguay the opportunity to edit the Newsletter about the predominant forage legume of the region. In addition to his permanent support, he has been so kind to provide us the original files of previous issues.
As new editor of the Newsletter, I would like to express my gratitude to the first editor Dr. William F. Grant; he has supported our initiative all the way through this edition.
Thanks to all for helping to update the recipient list. To Dr. Grant for the contacts in Canada, to Dr. Juan Sanjuán and Dr. Antonio Márquez for providing information about Spanish staff, to Dr. Sachiko Isobe, Dr. Martin Parniske, Dr. Francesco Damiani and Dr. Kevin Reed for making available links in Japan, England, Italy and Australia, respectively. I would like to express my particularly recognition to Dr. Jens Stougaard and Dr. Toshiki Uchiumi for providing extensive email list of Lotus researchers. Thanks to Dr. Mónica Barufaldi and Dr. Oscar Ruiz who provided their links to Argentinean researchers.
I would like to indicate that the session about Lotus literature is a merit of contributors. It is necessary to highlight the contribution of Dr. John Ayres, who supplied an extensive list of Australian bibliography, including referenced from other authors and to Dr. Judith Webb for sending her entire list of publications. Dr. Dmitry Sokoloff and Dr. Tatiana Kramina also sent a comprehensive list of Russian botanical bibliography. All this support, together with the volume of references already published in previous issues of Lotus Newsletter, will help to build up a reference database for the Lotus community.
What to contribute?
Send us the kind of information you would like to see in LN. - Contributions should be current, scholarly, and their inclusion well-justified on the grounds of new information. - Results of recently concluded experiments, recent additions to germplasm collections, information on new or tentative cultivars with descriptions. We want to include an
ii Lotus Newsletter (2003). Volume 33.
adequate cultivar description, including disease reactions and origin if possible. - Notes on acreage, production, varieties, diseases, etc., especially if they represent changing or unusual situations. - Genome maps and information on probe-availability and sequences, and populations synthesized for specific traits being mapped. Glossy black and white prints of maps should be included, if possible. Partial maps can also be submitted. - Short reports of workshops, conferences, symposia, field days, meetings, tours, surveys, network activities, and recently launched or concluded project. - Details of recent publications, with full bibliographic information and short reviews. - Personal news (new appointments, awards, promotions, change of address, etc.)
How to format contributions
- Include the full address with telephone, fax, and e-mail numbers of all authors. - Keep the items brief – remember, LN is a newsletter and not a primary journal. - Give the correct Latin name of every crop, pest, or pathogen at the first mention. - If possible, table should fit within the normal typewritten area of a standard upright page (not a ‘landscape’ page). You may include figures and photographs (black and white or color). Please send disk-files (with all the data) whenever you submit line figures and maps. - Supply the essential information: round off the data-values to just one place of decimal whenever appropriate, choose suitable units to keep the values small (e.g. use tons instead of kg). - All lists of references should have been seen in the original by the author and year. Provide all the details such as author/s, year, title of the article, full title of the journal, volume, issue, and page numbers (for journal articles), and place of publication and publishers (for books and conference proceedings) for every reference. Incomplete references will not be accepted. - The language of the Newsletter is English, but we will do our best to translate articles submitted in other languages. Authors should closely follow the style of the reports in this issue. Contributions that deviate markedly from this style will be returned for revision, and could miss the publication date. If necessary, we will edit communications so as to preserve a uniform style throughout the Newsletter. This may shorten some contributions, but particular care will be taken to ensure that the editing will not change the meaning and scientific content of the article. Wherever we consider that substantial editing is required, we will send a draft copy of the edited version to the contributor for approval before printing. - Contact the Editor for detailed guidelines on how to format text.
Material may be submitted at any time during the year. Deadline for Volume 34 will be 30th June 2004. Please send all contributions and request for inclusion in the recipients list to:
Monica Rebuffo, Lotus Newsletter Editor, c/o INIA La Estancuela, 70000 Colonia, Uruguay Fax +598 574 8012 Phone +598 574 8000 Email [email protected]
iii Lotus Newsletter. 2003. Volume 33.
Personalia
Dr. Carl Hoveland has informed the Editor that he retired September 1 and is now rehired on a half time basis to teach two courses and assist in some research and extension so will not be doing any work with Lotus in the future. Dr. Martin Blumenthal and Dr. G.M.P. Wilson, from Australia, Kjeld Marcker, from Denmark, Ken-ichi Suginobu, Japan, and Dr. Al E. Slinkard, Canada, also retired. All contributors to Lotus Newsletter will wish them a pleasant and productive retirement. We will look forward to any contribution on Lotus they may wish to send along.
Several Lotus researchers have been recently reassigned or no longer work on the subject. Dr. Jeffrey J. Steiner, U.S.A. and Dr. Greg Tanner, Australia, have been re-deployed and they are not actively doing research with Lotus any longer. Dr. James T. English, USA, has moved his research program completely to the soilborne pathogen, Phytophthora and he is no longer working with Lotus. Dr. Bernie Carroll is back in Australia, where he works on soybean. Dr. Susanne Freund, Germany, is no longer on legumes. Dr. Lone Bæk, Denmark left research for an administration position, whereas Dr. Christina Johansson, Dr. Niels Bech Laurensen and Dr. Jan-Elo Jørgensen from Denmark are working for the Industry at the present time. Dr. Iben Hansen and Dr. John Rasmussen, also from Denmark, moved to a teaching and medicine position, respectively. Dr. Maurice Jay, France, and Dr. Dale C. Darris, U.S.A., have changed research priorities and have no more interest on Lotus research. Aslaug Helgadóttir, Island, carries out very limited research on Lotus now adays. Dr. Alejandro J. Peralta, Uruguay, left to work in the seed industry. Dr. Mariana Kade, Argentina, has been reassigned to another crop.
I have recently received notice of the death of Dr. Daphne Fairey, Canada. International Herbage Seed Group, where she was an active member, has a web page In Memorial.
iv Lotus Newsletter (2003) Volume 33, 1-2.
The Lotus Newsletter 1970 (No.1) to 1985 (No.15)
This gives a brief outline of the Lotus Newsletter from 1970 to 1985 by the first Editor:
WILLIAM F. GRANT Emeritus Professor, P.O. Box 4000, McGill University, Macdonald Campus, Ste. Anne de Bellevue, Quebec H9X 3V9 Canada http://www.mcgill.ca/plant/faculty/grant/ http://eqb-dqe.cciw.ca/eman/ecotools/botanists/GrantWF.html
I thank Dr. Mónica Rebuffo for asking me to write a few words on how the Lotus Newsletter got started in 1970. In talking to colleagues there appeared to be interest in a Newsletter as many individuals were working on some aspect of Lotus research. I realized that a medium was required to let everyone know what research was being carried on in different parts of the world. This would prevent overlap of projects and form research alliances. I wrote letters (pre- Email) to all individuals who had published on some aspect of Lotus. The response for a Newsletter was highly enthusiastic. Thus, began the first newsletter covering all aspects of Lotus research from new species, new chromosome numbers, chemical constituents (such as HCN content), cytogenetics, hybridization studies, to Lotus species in agronomic practice such as breeding and pod shattering. In the first issue there were 21 contributors. The Newsletter consisted of a total of 30 mimeographed pages (pre-photocopying). The cover had an original drawing of Lotus corniculatus L. provided by one of my students.
The first Lotus Newsletter was sent to 71 individuals in 21 countries (Belgium, Bulgaria, Canada, Czechoslovakia, Denmark, England, France, Germany, Hungary, Israel, Japan, Netherlands, New Zealand, Poland, Portugal, Scotland, Spain, Sweden, Switzerland, Turkey and U.S.A.). Also to two contributors to the first issue from Australia and Uruguay made a total distribution to 23 countries.
The Lotus Newsletter was for an exchange of information where opinions in addition to established facts could be presented. Request for research material, personal news, new books of interest, meeting notices, letters to the editor, chromosome numbers published during the year, and recent publications, were some of the topic headings. In later issues I tried to have a lead article for each issue.
I published the Lotus Newsletter from 1970 (No. 1) to 1985 (No. 15). After 15 years, I thought someone else might assume Editorship and obtain financing for production and mailing costs. I was most pleased when Drs. Paul R. Beuselinck and Robert L. McGraw both
1 2 W. F. Grant agreed to become joint Editors starting Volume 16. (Present addresses, USDA-Agricultural Research Service, Plant Genetics Research Unit, Columbia, MO, USA, 65211; Dept. Agronomy, University of Missouri, Columbia, MO, USA, 65211, respectively). Under Dr. Beuselinck the Newsletter was published to volume 32, 2001, when he was requested to carry out other duties and could no longer publish the Newsletter (http://www.psu.missouri.edu/lnl/). I look forward to receiving the Lotus Newsletter published by the new Editor, Ing.Agr. Mónica Rebuffo.
Lotus Newsletter (2003) Volume 33, 3 – 5.
A preliminary study to explore potential of Lotus corniculatus L (Birdsfoot trefoil) in Palampur ( Himachal Pradesh, India)
SINDHU SAREEN and INDER DEV
Indian Grassland and Fodder Research Institute, Regional Research Centr, CSKHPKV Campus, Palampur 176062 (H.P.) India
Lotus corniculatus L (Birdsfoot trefoil), a forage crop, still longers to be explored for its potential. In eastern North America, it has emerged from an introduced weed in 1920s to become a highly successful forage crop (Grant and Marten, 1985). In NewZealand also its introduction into unimproved pasture has increased animal output, as it is non-bloating (Jones and Lyttleton, 1971) and is palatable in all seasons up to flowering (Armstrong, 1974). Its nutritive value compares favourably with other legumes when grown under good conditions (Waghorn and Shelton, 1992). Besides, it is better adapted than white clover to soils that are poorly drained, droughty or of low fertility (Hopkins et al., 1994). Despite all these advantages associated with it, no attention has been paid to popularize this crop. Therefore, a preliminary study was undertaken at this centre to explore the variability in native populations of Lotus.
Material and Methods
Seven populations of Lotus corniculatus, growing in and around Palampur, were identified for study. The details of these populations are given in table 1. these populations were studied for morphological parameters like no. of branches/plant, size of branch, no. of leaves /branch, no. of flowers/ branch, pods/ plant, seeds/ pod, s[read of the plant, root length, nodules/ root and biomass production. Five plants were marked in each population to record observations. In each population, five quadrates of 1x1meter were marked and number of Lotus plants in each quadrate was counted and biomass production was recorded. All the data was subjected to routine statistical analysis.
Results and Discussion
The data collected on various morphological parameters is given in tables 2 and 3. All the populations under study were prostrate and the plant spread ranged from 10 to 24 cm and plants in populations III and VII had maximum spread of 19.3 and 19.1cm. The plant count per quadrate ranged from 5 to 22 and average no. plants/ quadrate were maximum in population VII, being 19. Maximum no. of branches/ plant was recorded in population IV. The branches were 10cm (population I) to 17.3cm (III) long. Each branch was bearing 4 (V) to 7.2 (I) leaves and 0.6 (VI) to 1.8 (VII) flowers. Each plant had 21.4 (I) to 61.2 (VI) pods and each pod had 12.1 (IV) to 15.3 (V) seeds.
3 4 S. Sareen and I. Dev
The root length varied from 5.9cm (II) to 11.6cm (III) and no. of nodules/ root varied from 0.6 in population IV to 4 in population III. The dry biomass of a plant ranged from 3.7 gm (IV) to 17.8gm (VII) and below ground biomass ranged from 1.3gm (V) to 6.4 gm (VII).
The perusal of data reveals that significant variability among the populations was observed for all the parameters studied except leaves/ branch, flowers/branch, root length and nodules/root. Kelman and others (2003) also reported that genetic variance component for herbage yield, plant height and condensed tannins was significant in L.pedunculatus and L.corniculatus populations. They also reported strong association of characters with their geographic origin. The present study also reveals that the populations growing in abandoned field and bunds bear higher average values for most of the parameters studied. If these populations are exploited further for introduction into degraded pastures, the pasture productivity can be increased.
References
ARMSTRONG C.S. 1974. ‘Grassland Maku’ tetraploid lotus (Lotus pedunculatus Cav.). New Zealand Jour. Experimental Res., 2, 333-336.
GRANT W.F. and MARTEN G.C. 1985. Birdsfoot trefoil. In: Heath M.E., Barnes R.F. and Metcalfe D.S. (eds.) Forages: the science of grassland agriculture (4th ed), pp. 98-108.
HOPKINS A., DAVIES A. and DOYLE C. 1994. Lotus (Birdsfoot trefoil) (L. corniculatus and L. pedunculatus/ uliginosus). In: Clovers and other grazed legumes in UK pasture land, IGER Tech. Review no.1, pp. 15-17.
JONES W.T. and LYTTLETON J.W. 1971. Bloat in cattle 23. A survey of legume forages that do and do not produce bloat. NewZealand Jour. Ag. Res., 14, 101-107.
KELMAN W.M., BLUMENTHAL M.J. and HARRIS C.A. 2003. Genetic variation for seasonal herbage yield, growth habit, and condensed tannins in Lotus pedunculatus Cav. and Lotus corniculatus L. Australian Jour. Ag. Res., 48 (7), 959-968.
WAGHORN G.C. and SHELTON I.D. 1992. The nutritive value of lotus for sheep. Proc. New Zealand Soc. Animal Production, 52, 89-92.
Birdsfoot trefoil potencial in India 5
Table 1. Details of population sites. S.No. Site I Plum orchard II Open grassland III Field bund, near veterinary college, CSKHPKV, Palampur IV Field bund, CSKHPKV campus, Palampur V Protected grassland VI Abandoned field VII Abandoned field
Table 2. Data on morphological parameters in Lotus corniculatus populations. Site Plant No. of Length No. of No. of No. of No. of Root No. of spread tillers/ of tiller leaves/ flowers/ pods/ seeds/ length nodules/ (cm) plant (cm) branch branch plant pod (cm) root I 12.6 4.6 10.0 7.2 1.6 21.4 12.3 9.8 2.6 II 16.6 5.0 10.3 5.6 1.4 22.4 14.3 5.9 2.8 III 19.3 5.0 17.3 6.6 1.4 24.4 14.8 11.6 4.0 IV 17.0 6.4 13.0 4.6 1.2 58.0 12.1 8.4 0.6 V 14.2 4.6 11.4 4.0 0.8 45.4 15.3 7.4 3.6 VI 14.8 3.4 13.6 5.2 0.6 61.2 12.8 8.0 1.6 VII 19.1 5.0 15.9 5.2 1.8 55.4 14.4 10.7 3.6 CV% 19.43 43.09 26.13 40.44 78.22 31.83 13.08 38.58 92.88
Table3. Data on plant count/sqm and biomass production in Lotus populations Site Plant Biomass/ Below ground count –m2 plant (g) biomass/ plant (g) I 9.2 7.6 3.3 II 8.8 8.1 5.2 III 5.8 5.9 2.3 IV 14.0 3.7 2.0 V 13.4 4.2 1.3 VI 16.4 11.8 4.3 VII 19.0 17.8 6.4 CV% 20.77 22.29 50.6
Lotus Newsletter (2003) Volume 33, 7-8.
Lotus research update from Eastern Australia
JOHN F. AYRES Agricultural Research & Advisory Station, ‘Centre for Perennial Grazing Systems’, NSW Agriculture, PMB Glen Innes, New South Wales, Australia
In recent years, I have pursued 3 major studies on lotus improvement. The first project (‘Birdsfoot trefoil & Greater lotus in temperate perennial pasture’) investigated the potential of lotus-based pastures to improve grazing production in the high rainfall zone of eastern Australia and reflected on the respective zones of adaptation of birdsfoot trefoil and Greater lotus. The second project (‘New lotus varieties for acidic soils in northern NSW’) characterised birdsfoot trefoil germplasm for traits associated with adaptation under short photo-period conditions and identified promising selections for subsequent breeding work. The third study (‘Development of birdsfoot trefoil cultivars for permanent pastures in the northern recharge zone’) is currently developing experimental varieties of birdsfoot trefoil. Highlight findings include:
‘Birdsfoot trefoil & greater lotus in temperate perennial pasture’ (1994 - 2000). The adaptation of greater lotus and birdsfoot trefoil to low fertility acidic soils and the presence of condensed tannins in lotus foliage make greater lotus and birdsfoot trefoil potentially valuable legumes for the Australian grazing industries. However, use of lotus in Australian pastures has been limited by a) lack of knowledge of the respective zones of adaptation of Greater lotus and birdsfoot trefoil, and b) lack of adapted varieties. To bridge this knowledge gap, I led a state- wide study in NSW comprising a grazing experiment replicated in 4 environments (Northern Tablelands, North Coast, Southern Tablelands and South Coast) supported by co-learning studies at 17 farm sites in coastal and tablelands districts. The experiment provided results for the establishment and management of lotus–based pastures and the expression of persistence mechanisms in these 4 environments. The co-learning phase demonstrated the adaptation of lotus to a diversity of climatic, edaphic and enterprise applications across the high rainfall zone. This project achieved significant outcomes including: • Improved definition of the lotus zone – Greater lotus for high rainfall coastal districts and niche hinterland and tablelands sites, and birdsfoot trefoil as a new alternative legume for northern NSW. • Increase in the knowledge base of lotus technology – establishment requirements, management practices for persistence and cultivar evaluation results. • Increased farmer awareness of the unique and valuable properties of birdsfoot trefoil – drought tolerance and adaptation to low fertility acidic soils, potential to lift the productivity of marginal grazing lands and to increase water use on acidic soils in dryland salinity recharge areas. • The project identified the need for development of a broad adaptation Greater lotus cultivar and a short photo-period birdsfoot trefoil cultivar.
7 8 J. F. Ayres
‘New lotus varieties for acidic soils in northern NSW’ (1999 - 2001). The most significant result of my research with lotus to this point indicated that the northern limit to the zone of adaptation of birdsfoot trefoil in Australia is determined by photo-period. Moreover, I concluded that if widespread adoption were to be accompanied by increased susceptibility to fungal diseases (as experience in North America has indicated), maintenance of population density by seedling recruitment will also be essential for long term persistence.
Consequently, the aim of this study was to characterise a world-sourced set of birdsfoot trefoil lines and undertake selection for flowering prolificacy. This work developed data-sets of vegetative and reproductive traits for some 50 birdsfoot trefoil lines, collected ca. 70 elite genotypes, and determined the genetic variability and heritability of seed yield components (flower/tiller, umbels/tiller, pods/umbel, seeds/pod) in these populations to validate a breeding project to follow. One birdsfoot trefoil breeding line and 4 selected populations were identified as suitable for progressing to experimental variety status.
‘Development of birdsfoot trefoil cultivars for permanent pastures in the northern recharge zone’ (2002 -2005). Work in the previous 2 projects identified elite birdsfoot trefoil germplasm. At this stage of the birdsfoot trefoil improvement program, I was commissioned by the Dryland Salinity CRC ‘…to progress birdsfoot trefoil germplasm to experimental variety status by 2005 to achieve a new deep-rooted perennial legume for the upper catchment Murray-Darling Basin’.
This is a current project in which I am polycrossing selected genotypes to form breeding lines, characterising the breeding lines, and evaluating genetic gain in Syn 1 and Syn 2 populations against the unselected population. Preliminary data to hand comparing the seed yield components of the selected genotypes against the unselected population shows dramatic genetic gain from 2 rounds of recurrent selection. Lotus Newsletter (2003) Volume 33, 9 – 10.
Differences in pollen fertility and seed quality between diploid and tetraploid cytotypes of Lotus glaber Mill.
MÓNICA S. BARUFALDI 1, M.CRISTINA DE PABLO1, RAÚL H. RODRÍGUEZ1,2, M.VIRGINIA MONTAGNA1, RUBÉN D. PÉREZ1, LORENA DAULERIO1 and ESTEBAN HIDALGO1
1Facultad de Agronomía de Azul, UNCPBA, Argentina. 2 Dpto. de Agronomía, EEA INTA – Balcarce, Argentina.
The research group of Genetics and Plant Breeding and Agricultural Botany of the Faculty of Agronomy of Azul (UNCPBA) began in 1998, within the research project "Bases for the genetic improvement of Lotus", a series of activities starting from which a tetraploid population (2n=4x=24) was obtained by using colchicine on diploid seedlings (2n=2x=12) of L. glaber Mill. (Barufaldi et al., 1999; Barufaldi et al., 2000).
The present work compared some characteristics related to seed quality and pollen fertility in a tetraploid population and in the diploid variety named Chajá Tresur. The 1000-seed weight and the percentage of fertile pollen grains, seed germination, hard seeds, dead seeds, fresh seeds and abnormal seedlings were evaluated in both cytotypes.
In January 2001, 22 plants of Chajá Tresur and 49 of the tetraploid population were selected for study. Two inflorescences per plant were picked at random and two flowers per inflorescence were utilised to measure pollen fertility. Pollen grains were stained according to the technique of Alexander (1980) and at least 200 pollen grains per flower were examined.
For the determination of 1000-seed weight, four samples of seeds were taken in each cytotype and underwent pre-refrigeration and mechanical scarification. One hundred seeds were distributed in each tray containing a humid substratum. Then, they were located in a germination camera at 20-30°C following the ISTA protocols. The trays were arranged according to a completely randomised design with four replicates. T-test was performed to compare the means of the characteristics analysed in both cytotypes.
Highly significant differences were detected between Chajá Tresur and the tetraploid population in pollen fertility, 1000-seed weight, seed germination and dead seeds (Table 1). Pollen fertility and seed germination were significantly higher in Chajá Tresur, while 1000- seed weight and dead seeds were significantly higher in the tetraploid population.
In relation to the percentages of hard and soft seeds, as well as abnormal seedlings, significant differences were not detected between tetraploid population and Chajá Tresur, indicating that the asexual poliploidization did not present deleterious effects on these characters.
9 10 M. S. Barufaldi et al.
Table 1. Comparison of characters between Chajá Tresur and a tetraploid population of L. glaber Mill.
CHAJÁ TETRAPLOID MEAN CHARACTER TRESUR§ POPULATION§ DIFFERENCES
Pollen fertility 89,17 ± 8,18 68,88 ± 9,14 *** (%)
1000-seed weight 1,01 ± 0,05 1,68 ± 0,07 *** (g)
Seed germination 81,00 ± 2,44 65,50 ± 6,75 ** (%)
Hard seeds 13,75 ± 2,87 14,25 ± 6,94 n.s. (%)
Soft seeds 1,25 ± 0,95 4,00 ± 2,88 n.s. (%)
Dead seeds 1,50 ± 0,57 15,00 ± 1,29 *** (%)
Abnormal seedlings 2,50 ± 1,00 1,25 ± 0,50 n.s. (%)
§ Results are given as mean values ± standard deviation n.s.: Non significant differences P< 0,05 **, ***: Significant differences at P< 0,01 and P< 0,001, respectively
References
ALEXANDER M.P. 1980. A versatile stain for pollen, fungi, yeast and bacteria. Stain Technol., 55 (1), 13-18.
BARUFALDI M.S., CROSTA H.N., ESEIZA M.F. y RODRÍGUEZ R.H. 1999. Autotetraploides inducidos en Lotus tenuis Waldst. et Kit. Actas XXIX Congreso Argentino de Genética - XXXII Congreso de la Sociedad de Genética de Chile y III Jornada Chileno -Argentina de Genética. Rosario, Santa Fe. 5 - 8 de setiembre, p. 348.
BARUFALDI M., ANDRÉS A., CROSTA H. y ESEIZA M. 2000. Obtención de una población autotetraploide de Lotus glaber Mill. (Lotus tenuis Waldst. & Kit). Revista de Tecnología Agropecuaria INTA Pergamino, V (15), 45-50.
Lotus Newsletter (2003) Volume 33, 11 – 18.
Insect pests damaging Lotus corniculatus L. flowers and seeds in Uruguay.
ROSARIO ALZUGARAY
Instituto Nacional de Investigación Agropecuaria, INIA La Estanzuela, Colonia, Uruguay
Many insects feed on Lotus corniculatus foliage in Uruguay. Some of them can sporadically cause severe defoliation; such is the case with some cricket species, especially under dry weather conditions. In general, plants overcome the damage and insecticide control is not frequent.
When birdsfoot trefoil fields are planned for seed harvesting, two insect species become a major problem, because of the rapid increase of its populations during the flowering period and the behavior and feeding habits of both species that complicate the early detection of the attack. They are Epinotia aporema (Lepidoptera: Tortricidae), a bud borer, and the seed chalcid Bruchophagus platypterus (Hymenoptera: Eurytomidae).
Epinotia larvae bore into the flowers sticking them together, while feeding on the reproductive structures. The seed chalcid adults lay the eggs into the pods, one per seed, and the larvae feed on the cotyledons completely destroying the newly formed seed. Damage of both species can be easily oversight and, in addition to this, the use of chemical control is limited for the dependency from pollinators in order to achieve the best yields.
Damage assessments of these insect pests were carried out at INIA La Estanzuela for several years. The results help in designing strategies for the management of the pests.
Seed chalcid fly Bruchophagus platypterus (Walker) (Hymenoptera: Eurytomidae)
Three different species of chalcids damage the main forage legume species around the world. Their appearance, behavior and habits are similar but the insect – host plant relationships are very specific (Table l).
11 12 R. Alzugaray
Table 1. Seed chalcid species and the plant they parasitize.
Host plant Seed chalcid species
red clover Bruchophagus gibbus Boheman
birdsfoot trefoil Bruchophagus platypterus (Walker)
alfalfa Bruchophagus roddi Gussakovskii
Carámbula (1981) reported the three species present in Uruguay. The high number of adults of Bruchophagus platypterus (the chalcid species that attacks birdsfoot trefoil) worried farmers in the 1981/82 season (Etcheverry and Morey, 1982). It has also been reported damaging Lotus tenuis in Argentina with a range of incidence between 0 – 24% (Mujica, 1987). The species damaging alfalfa was first reported in the country at La Estanzuela in the 1967 season by Pritsch (1967), causing losses he estimated in as much as 20 – 30%. In the season 1970/71, Castells had found one of the chalcids in Medicago polymorpha var confinis seeds (Etcheverry and Morey, 1982).
They are minute wasps, around 1.5 – 2.5 mm. Their size is related with the size of the seed they parasitize, a bit larger the one that attacks red clover than the species in birdsfoot trefoil. It is difficult to see them without a lens, and it is also difficult to observe their damage. The larvae have chewing mouthparts.
At the beginning of the spring the adults emerge from seeds of the previous season that are in the soil. They look for recently pollinated flowers and deposited their eggs in the newly formed seed, one egg per seed. The larvae live inside the seed feeding on the cotyledons, so that when the healthy seeds mature, in the attacked seeds there is just a larva inside the teguments (Figure 1; Batiste, 1967). The developing period is the same of the seed, being shorter as the season progress (Ahring et al., 1984). There are three or four generations per year. In the field the only symptoms of the attack are small holes in the mature pods, and a yield lower then expected.
Damage assessment
The evaluations were made collecting mature pods in commercial fields from late December to March (since the beginning of the season to the last probable harvesting date). The sample included 90 umbellas per date that were manually thrashed in the lab. Health and damaged seeds were sorted and counted. The damage was calculated as a proportion of total seed [damaged seeds/(damaged plus sound seeds)* 100] (Pippolo, 1998; Alzugaray, in press). The results are shown in Figure 2.
Insect pests in birdsfoot trefoil 13
Figure 1. Seed chalcid Bruchophagus platypterus adult emerged from Lotus corniculatus seed.
50 1991-92 1992-93 1993-94 1994-95 1995-96 1996-97 1997-98
40
e
g 30 a m 20 % da
10
0 515212345678910111213week Dec Jan Feb Mar
Figure 2. Seed chalcid (Bruchophagus platypterus) percent damage to Lotus corniculatus in succesive seed production years at La Estanzuela (Colonia, Uruguay). Damage=[damaged seeds/(damaged plus healthy seeds)* 100]. Week 52 – last week of december, Week 1 – first week of January
The damage caused by the seed chalcid to birdsfoot trefoil in Uruguay seems to be in a very stable situation. The damage exceeded the 20% only in a few occasions, being generally below the 10%. The explanation for this is the presence of another minute wasp that parasitizes the seed chalcid, diminishing its populations from one generation to the 14 R. Alzugaray following. The natural enemy has been identified as Tetrastichus bruchophagi (Hymenoptera, Eulophidae) (Alzugaray, 1991).
The population of the parasitoid has also been monitored and the relation parasitoid/seed chalcid can sometimes exceed the 10/1 ratio (Figure 3). The abundance of the parasitoid is evident at the end of the season.
60 natural control
d
i
c 50 l a h c 40 d e e
s 30 enn/
. 20 nat o
i t ra 10
0
Dec Jan Feb Mar Apr Figure 3. Natural control of Tetrastichus bruchophagi on Bruchophagus platypterus, La Estanzuela, Uruguay, 1991 – 1999. Each point indicates the ratio natural enemy/seed chalcid adults emerged from the seed samples (After Alzugaray, in press).
Epinotia Epinotia aporema Wals. (Lepidoptera: Tortricidae)
Epinotia aporema larvae have been reported in Uruguay feeding on red clover, alfalfa, birdsfoot trefoil, soybeans, grain legumes, Lotononis bainesii and Vicia spp. (Bentancourt and Scatoni, 1989; Alzugaray, in press)
Its presence is known in Uruguay since the ’70s as a pest of beans (Morey, 1972). It was in the ‘80s, when soybean area increased, that Epinotia became a problem in production systems with soybean and pastures.
Insect pests in birdsfoot trefoil 15 The adults are small moths ca. 10 mm long (Figure 4). They lay the eggs on the foliage. The larvae with chewing mouthparts feed on leaf and flower buds, sticking the folioles together (Figure 5). The life cycle takes 35 – 40 days depending on the temperature (Table 2). It is a pest of alfalfa, red clover and birdsfoot trefoil as well as grain legumes such soybeans.
Table 2. Epinotia aporema life cycle at temperatures between 21 - 24ºC (Morey, 1972)
Days pre – oviposition 2 egg 5 larva 14 – 20 pupa 14 – 15 total 35 - 42 Figure 4. Adult female of Epinotia aporema.
Figure 5. Field symptoms of the attack of Epinotia aporema in Lotus corniculatus flowers (left damaged, right healthy flower).
Damage assessment
Full developed larvae (5th instar) were individually caged and offered a known number of flowers. Damage was assessed after 4 days as the number of flowers completely destroyed over the total offered.
The results indicate that a 5th instar larva feeds an average of 2.2 lotus flowers per day, within a range of 1.5 to 3.5 flowers. An estimated population of 150 larvae per m² would 16 R. Alzugaray destroy 375 flowers per day and in 10 days 3750 flowers of 5000 that can be assumed per m² (Alzugaray, in press). This is a very conservative assumption because it is frequent to find more than a larva per umbella inside the case they form while sticking flowers and leaves.
Population fluctuations
Populations of Epinotia adults have been recorded weekly since 1989 at INIA La Estanzuela using a black light trap. The curves can be observed in Figure 6..From the point of view of seed harvesting, it does not have the same meaning to register an important capture of adults in November (first flowering) than in February, when the seed may not be harvested.
The relation between adult captures and larvae populations in the field was surveyed making plants sampling and counting larvae in the buds. The results confirm that, once the adult captures in the light trap increase, there is a period of two weeks to get prepared for high larvae populations in the field (Zerbino and Alzugaray, 1998). On these bases the use of physiological insecticides can be recommended, using light trap data as a warning.
1989-90 1990-91 1991-92 1992-93 1993-94 1994-95 1995-96 1996-97 1200 1997-98 2000-01 2001-02
800 d e ur pt a c s t ul d
a 400
0 36 39 42 45 48 51 1 4 7 10 13 16 19 22 25 28 31 34 week
Sep Oct N ov D ec Jan F eb M ar A pr M ay Jun Jul A u g
Figure 6. Weekly captures of Epinotia aporema adults in black light trap, La Estanzuela, 1989-2002. (Modified from Zerbino and Alzugaray, 1998)
Final comments
Both insect species, the seed chalcid and epinotia, damage the Lotus corniculatus seed crops, attacking directly the reproductive structures of the plants. The early detection of the attacks is seldom successful and in both cases the control of the pest presents actual difficulties.
Insect pests in birdsfoot trefoil 17 It is important to realize that both species occur simultaneously, at flowering time, and that any measure to control one of them will affect the other insect situation. Chemical sprays to control epinotia damage would probably endanger the natural control that maintains the seed chalcid populations in the level of sporadic problem.
Bibliography
AHRING R.M., MOFFETT J.O. and MORRISON R.D. 1984. Date of pod-set and chalcid fly infestation in alfalfa seed crops in the southern Great Plains. Agronomy Journal, 76, 137 - 140.
ALZUGARAY R. 1991. Avispita del lotus. In Indarte E. and Restaino E. (eds.) Pasturas y producción animal en áreas de ganadería intensiva. Montevideo, INIA. Serie Técnica N° 15, p. 43-47.
ALZUGARAY R. 1998. Bruchophagus platypterus. In Scatoni and Bentancourt (eds.) Guía de insectos y ácaros de importancia agrícola y forestal en el Uruguay. Montevideo, Facultad de Agronomía, PREDEG, GTZ. 2 p.
ALZUGARAY R. (in press). Daños por insectos en la producción de semilla de leguminosas forrajeras. INIA Serie Técnica.
ALZUGARAY R. and ZERBINO M.S. 1998. Daño de Epinotia aporema (Lepidoptera: Tortricidae) en trébol rojo (Trifolium pratense) y lotus (Lotus corniculatus). In Congresso Brasileiro de Entomologia (17., Rio de Janeiro, Brasil). Resumos. SEB. p. 555.
BATISTE W.C. 1967. Biology of the trefoil seed chalcid Bruchophagus kolobovae Fedoseeva (Hymenoptera: Eurytomidae). Hilgardia, 38 (12), 427-469.
BENTANCOURT, C. M., SCATONI, I. B. 1989. Lepidopteros de importancia económica en el Uruguay. Montevideo, Fac. Agronomía. Nota Técnica nº 7. 57 p.
CARÁMBULA M. 1981. Producción de Semillas de Plantas Forrajeras. Montevideo, Hemisferio Sur. 518 p.
ETCHEVERRY, A.; MOREY, C. 1982. Una plaga alarmante : la avispa de la leguminosa. La Mañana (Montevideo), 12 de abril, p. 28.
MOREY C.S. 1972. Biología y morfología larval de Epinotia aporema (Wals.) (Lepidoptera: Olethreutidae). Montevideo, Fac. Agronomía. Boletín N° 123. 14 p.
MUJICA M.M. 1987. Presencia de Bruchophagus platypterus Walker en la República Argentina. Infestación de semillas de Lotus tenuis Waldst et Kit. Rev. Fac. Agron. - La Plata 63: 82 - 90.
18 R. Alzugaray
PIPPOLO L. 1998. Incidencia de la avispita Bruchophagus platypterus Walker (Hymenoptera, Eurytomidae) en la producción de semilla de Lotus corniculatus L. en diferentes fechas de floración. Montevideo, Facultad de Agronomía. Tesis Ingeniero Agrónomo. 39 p.
PRITSCH, O.M. 1967. Informe trimestral (enero-marzo 1967). Uruguay. CIAAB. 6 p.
ZERBINO M.S. and ALZUGARAY R. 1998. Captura de adultos de Epinotia aporema (Lepidoptera: Tortricidae) en trampa de luz y su relación con la población de larvas en el campo. In Congresso Brasileiro de Entomologia ((17., Rio de Janeiro, Brasil). Resumos. SEB. p. 488. Lotus Newsletter (2003) Volume 33, 18 – 24.
Studies of rust fungi on Lotus subbiflorus and L.uliginosus
JAVIER CILIUTI, SEBASTIÁN ARRIVILLAGA, SILVIA GERMÁN, SILVINA STEWART, MÓNICA REBUFFO and SEBASTIÁN HERNÁNDEZ.
Instituto Nacional de Investigación Agropecuaria, INIA La Estanzuela, Colonia, Uruguay.
Introduction
Root and crown diseases reduce persistency of legumes, whereas foliar diseases usually interfere with the normal functions of the leaf. Sometimes they cause defoliation, which reduces yield and quality of forage. Altier (1997) has done an extensive research in diseases of Lotus corniculatus L. in Uruguay. The survey demonstrates that the incidence and severity of leaf diseases vary with the seasons and climatic conditions. The most prevalent diseases (Phoma, Colletotrichum, Phomopsis and Cercospora) developed mostly in spring, whereas rust caused by Uromyces spp. can be found from February to April.
L.corniculatus has been the predominant legume in Uruguayan pastures for the last four decades. Most of the locally adapted cultivars (San Gabriel, Estanzuela Ganador, INIA Draco) have good resistance to Uromyces, suggesting that the breeding program selected against this trait. The area of the annual Lotus subbiflorus has increased greatly during the last decade in Uruguay, expanding to adjacent regions of Brazil and Argentina. More recently, Lotus uliginosus became another species with great potential for rangelands. Foliar diseases on L. subbiflorus and L. uliginosus have not been extensively studied.
In early November 2000, rust was observed on L. subbiflorus at Palo a Pique Experimental Field, Treinta y Tres, Uruguay (V.Olivieri, INIA internal report, 2001). Rust severity ranged from trace to moderate at several other plot sites within a 90 km radius. Only the uredinial stage of the fungus has been observed. Uredinia were mostly found on leaflets, but petioles and stems were also infected. The following year (2001) rust was prevalent in epidemic proportions on seed production plots, where all the lower leaves were killed and stem infections caused the distal parts to wilt and die. The disease may appear at any time during the growing season when warm and wet weather prevails, but it develops most abundantly at seedling stage in early autumn and at flowering stage in late spring.
Rust was also recorded on L. uliginosus cv. Grassland Maku in 2001, when severity ranged from trace to low. The disease severely damaged spaced plants the following year (2002) at La Estanzuela, Colonia. Uredinia were observed in leaflets and stems, which turned yellow and drop or dried off. Maximum damage was observed during dry spells, when vegetative growth was stunted and pustules developed up to the top leaflets. Neither the presence of telia has been verified on L. uliginosus nor L. subbiflorus.
18 Rust on Lotus 19
Field observations of the pathogen have been reported on several Lotus species worldwide as well as in the region, among other forage legume species. Zeider (1985) reported rust of epidemic proportion on L. corniculatus in USA and Uromyces striatus f. medicaginis has been reported as a miscellaneous disease on L. subbiflorus in Hawaii (Gardner, 1994). Regional reports of the disease refer to U.loti (U. euphorbiae-corniculati) on Lotus glaber in Argentina (Juan et al., 2000) and on L. corniculatus in Uruguay (Altier, 1997).
The literature quotes rust on the genera Lotus to belong to a number of species and/or physiological races which differ in their ability to attack different host genera and species and different varieties within a species. Arthur (1934) identified two species of rust (U.striatus loti and U. punctatus) collected on the alternative host Euphorbia cyparissias as the pathogen that causes the disease on L. corniculatus. In addition to this ornamental plant, aecia and pycnia are produced on some congeneric species in Europe. The occurrence of 6 species of Uromyces in the tribe Loteae is reported by El-Gazzar (1981). Broad-spectrum species of the disease included U. anthyllidis, U. striatus, U. pisi and U. genistae-tinctoriae, reported in 7 Tribes in addition to Loteae, whereas U. euphorbiae-corniculati is reported on Vicia and Lotus. On the opposite, U. loti is specific of the genera Lotus and Tetragonolobus.
In addition to the importance of the rust outbreak on two Lotus species in Uruguay, rust has developed on cultivated soybean in some regions of the Southern Cone. The lack of information on the host range of the disease in the country determined the need to begin a series of greenhouse test to identify the rust species/races. A differential set with different Lotus species was designed for this purpose by the forage research group and the Cereal Disease Laboratory at INIA La Estanzuela in 2002. The present work compared cross- inoculations of rust collected in either cultivated or endemic species of Lotus in Uruguay, aimed to identify the host range in cultivated forage legume species.
Materials and Methods
Urediniospores from L. uliginosus cv Grassland Maku (Strain 1) and L. subbiflorus cv El Rincón (Strain 2) were collected at La Estanzuela, Colonia and La Carolina, Flores, respectively. Strain 3 was harvested from spontaneous plants of L. corniculatus in the surroundings of La Estanzuela. Inoculation tests were carried out on L. corniculatus cv. San Gabriel, L. uliginosus cv. G.Maku, L. subbiflorus cv. El Rincón, Medicago sativa cv Estanzuela Chaná, Trifolium pratense cv Estanzuela 116 and Trifolium repens cv Estanzuela Zapicán (Table 1).
Rust collection was done with a cyclone spore collector (Cherry and Peer, 1966) and urediniospores were vacuum dried and stored under refrigeration (4°C). Pots containing 8-10 seedlings at the stage of 5-8 leaflets were inoculated by spraying with a urediospores suspension in Soltrol 170 (Phillips Petroleim, Bartlesvill, OK) mineral oil, a technique used for inoculations of wheat rust in greenhouse tests (Rowell, 1984). A total of 9 replications per species were evaluated. In addition, one pot of soybean was inoculated with each strain.
20 J. Ciliuti et al.
Plants were located in a humid and dark chamber during 24 hours after inoculation. First symptoms of the disease appeared approximately 10 days after inoculation and rust rating was scored in the most severely infected leaflet on each plant 14 days after inoculation. The scale follows the one described by Skinner and Stuteville (1995): resistant (no symptoms), moderately resistant (flecks and closed pustules), moderately susceptible (closed pustules and small open pustules), susceptible (small open pustules), highly susceptible (medium to large open pustules).
Results and Discussion
Common varieties of rust that attack clovers cannot be distinguished on the basis of symptoms but can be differentiated by their capacity to infect the various legumes. The present study suggests that all three strains were specific to the genera Lotus, since neither infected the cultivars of Medicago sativa, Trifolium repens or T. pratense tested (Table 1). This specificity is supported by research developed by Zeiders (1985) in USA, who mentions Uromyces striatus var. loti as the possible causal fungi causing Lotus leaf rust. Furthermore, Skinner and Stuteville (1995) and El-Gazzar (1981) reported strains of U. striatus, found as pathogens of alfalfa, being capable of surviving and reproducing on a broad range of plant species (including Trifolieae and Vicieae), but no susceptibility was found in the tribe Loteae (L. corniculatus, L. uliginosus, L. angustissimus) by Skinner and Stuteville (1995).
Table 1. Cross-inoculation reaction of rust strains.
Strain 1 Strain 2 Strain 3 Lotus uliginosus ++ + - Lotus angustissimus + - - Lotus subbiflorus - ++ - Lotus corniculatus - - ++ Medicago sativa - - - Trifolium pratense - - - Trifolium repens - - - ++ highly susceptible + moderatelly susceptible - resistant
Rust on L. uliginosus (Strain 1) infects L. uliginosus (Figure 1) and L. angustissimus. The rust on L. subbiflorus (Strain 2) infects also two species (L. subbiflorus – Figure 2 and L. uliginosus –Figure 3), whereas Strain 3, collected on L. corniculatus, was specific for the species (Figure 4). No references about cross-inoculation within the genera Lotus have been found in the literature to support these results. However, Medicago and Trifolium rusts are good examples of case studies where one strain could infect several species of the same genera (Skinner and Stuteville, 1995; Hanson and Kreitlow, 1953). Reactions of Strain 1 and Strain 2 were more susceptible in the original species where the samples were collected than in the Rust on Lotus 21 other host species, which had moderately susceptible reactions (Figures 1 and 3). Rust strain mixtures were discarded by single pustule isolations inoculation on cross species.
Figure 1. Strain 1 on underside of Figure 2. Strain 2 on upperside of L. uliginosus leaflets. L. subbiflorus leaflets.
Figure 3. Strain 2 on upperside of Figure 4. Strain 3 on underside of L. uliginosus leaflets. L. corniculatus leaflets.
Symptoms on L. subbiflorus are usually limited to the leaflets in the greenhouse inoculations, although they may occur anywhere on petioles and stems. The earliest symptom is the development of minute, light-yellow spots, mostly on the upperside leaflet surface, similar to the early symptoms of other leaf spot diseases. Spots enlarge and become pustules, mostly oval, developing on either leaflet surface. Single fully developed pustules measure about 0.5-1 mm across, although they are sometimes arranged in circles around a single pustule. When pustules are abundant the entire leaflet may turn yellow, die and fall off. Symptoms on L. uliginosus are slightly different. Pustules are seen mainly in the underside of the leaflets and in very severe cases in stems, under the shape of small circular light brown color pustules that break the epidermis and expose the urediniospores. No telial state has been found in both species.
22 J. Ciliuti et al.
Phakopsora pachyrhizi was descarted as the causal pathogen, since Lotus rust has no paraphyses, uredia on both sides of the leaflets and on stems, and urediniospores with thick walls (1.5 to 2 µ), features that do not match with P. pachhyrhizis (Ono et al, 1992) Phakospora pachyrhizi. Furthermore, none of the strains developed symptoms on soybean in the greenhouse.
Isolates used may not represent the whole rust population present in the country. Moreover, the present study has a narrow range of host species and cultivars. For those reasons, these results should be considered as representing the minimum host range that a fungus causing Lotus rust may have. Understanding the fungus life cycle and host range will provide an insight of the potential risk of the disease. From an epidemiological standpoint, results suggest that an outbreak of L. uliginosus rust may originate on this species or arise from at least two other species (L. angustissimus and L. subbiflorus). Although the relative importance of these species in the epidemiology of L. uliginosus is unknown, it is now clear that they are capable of playing a significant role in innoculum increase and dissemination. There are no reports in the literature that explore the pathogen host range within the genera Lotus to support these research findings.
Screening for resistance to Strain 1 has allowed to identify resistant plants in L. uliginosus. Further studies should address to a wider pathogen sampling, with isolates from different areas, as well as an increased range of host species and cultivars within species, in order to know the pathogen spectrum.
Acknowledgements Nora Altier and Jim Groth for their advice on the general approach of the research and Richard García for his support on the greenhouse testing.
References
ALTIER N. 1997. Enfermedades del Lotus en Uruguay. INIA, Montevideo. Serie Técnica N° 93, 16 p.
ARTHUR J.C. 1934. Manual of the rusts in United States and Canada. Purdue Research Foundation, Lafayette, Indiana, USA. p. 299-300.
CHERRY E. and PEET C.E. 1966. An efficient device for the rapid collection of fungal spores from infected plants. Phytopatology, 56, 1102-1103.
EL-GAZZAR A. 1981. Systematic implications os susceptibility to Uromyces rusts in Leguminosae. In Polhill R.M. and Raven P.H. (eds) Advances in Legume Systematics. Royal Botanical Gardens, Kew, England. p. 979-994.
GARDNER D.E. 1994. The native rust fungi of Hawaii. Canadian Journal of Botany, 72, 976– 989.
Rust on Lotus 23
HANSON E. W. AND KREITLOW K. W. 1953. Grasses and legumes. The many ailments of clover. Yearbook of Agriculture, USDA, Washington DC, USA. p 217-228.
JUAN V.F., MONTERROSO L., SACIDO M.B. and CAUHÉPÉ M.A. 2000. Postburning legume seeding in the Flooding Pampas, Argentina. Journal of Range Management, 53, 300- 304.
ONO Y., BURITICÁ P. and HENNEN J.F. 1992. Delimitation of Phakopsora, Physopella and Cerotelium and their species on Leguminosae. Mycology Research, 96, 825-850.
ROWELL J. B. 1984. Controlled Infection by Puccinia graminis f sp. tritici under artificial conditions. In: BURSHELL W. R., ROELFS A. P. (ed) The cereal rusts. Origins, specifity, structure, and physiology. Academic Press, Orlando, USA. Vol 1:291-332.
SKINNER D. Z. and STUTEVILLE D. L. 1995. Host range expansion of the alfalfa rust pathogen. Plant Disease, 79, 456-460.
ZEIDERS K. E. 1985. First report of rust caused by Uromyces species on birdsfoot trefoil in the United Status. Plant Disease, 69, 727.
Lotus Newsletter (2003) Volume 33, 25 -36.
Lotus activities: Background and present research
Rosario Alzugaray Peter Paľove-Balang Ana Arambarri Martin Parniske Søren Bak Mónica Rebuffo Mónica Susana Barufaldi J.S. Grant Reid María Bemhaja William John Rogers Omar Borsani Oscar Adolfo Ruiz Miguel Cauhépé Federico Sanchez Maurizio Chiurazzi Juan Sanjuán Daniel H. Cogliatti Toshiki Uchiumi Quentin Cronk Osvaldo Ramón Vignolio Hilda Nélida Crosta Jeffrey J. Volenec Francesco Damiani Judith Webb Jose Pedro De Battista Guilhem Desbrosses Pedro Díaz Gadea Marcelo Francisco Eseiza Rosario Alzugaray Osvaldo Néstor Fernández URUGUAY Henk Franssen Gabino Garcia de Los Santos I have been working for 18 years on insect pests Alicia Grassano of L. corniculatus as well as other forage Peter Gresshoff legumes (red clover, white clover, alfalfa) with Qunyi Jiang emphasis on insect species damaging flowers and seed production. The main projects I Kyung-Nam Kim worked on are related to Epinotia aporema Tatiana E. Kramina (Lepidoptera, Tortricidae) a bud borer, and Pedro Laterra Bruchophagus paltypterus and B. gibbus, Viviana Lepek (Hymenoptera, Eurytomidae), seed chalcids. Lina A.C. Lett Currently I am also interested on aphids and Dasharath Prasad Lohar Halticus pygmaeus (Hemiptera, Miridae) a Antonio J. Márquez garden flea-hopper that kills white clover Jim H. McAdam seedlings. The research results, that include Jorge Monza damage levels, chemical and natural control and Takuji Ohyama monitoring strategies for the different species, Fernando Olmos are published only in Spanish. Alicia Orea María Cristina de Pablo
25 26 Lotus activities.
biochemical studies. Furthermore, this Ana Arambarri germplasm will be evaluated with the aim of ARGENTINA obtaining an improved population that contemplates the possibility of obtaining We recently published two works about leaf tetraploid cultivars, selected for the pastureland epidermal microcharacters of the Old World conditions of the Depressed Pampa of the species of Lotus and their systematic Province of Buenos Aires. I will engage in the significance. We continuous working on the evaluation of tetraploid plants of different traits, taxonomy of the New World species of Lotus, such as, forage production, seed set and seed including studies on their epidermal quality. characteristics of the subgenus Acmispon. These species are knowledge as: Acmispon, Hosackia and Syrmatium. María Bemhaja URUGUAY Søren Bak I have been working with L. corniculatus, L. DENMARK glaber and L. uliginosus under native pasture improvement on basaltic and conventional Biosynthesis and function of cyanogenic improvement on sandy soil in the North Area of glucosides and nitrile glucosides in higher Uruguay, since 1980. Research Program has plants. Evolutionary aspect of biosynthesis of been leading with persistence and physiological secondary metabolites and in particular the behavior and fitness (evaluation and systemic phylogeny of cytochromes P450 and agronomic management), in small paddocks and glycosyltransferases family 1, for further details under grazing systems. see http://www.biobase.dk/P450 (The
Arabidopsis P450, cytochrome b5, P450 reductase, and Glycosyltransferase Family 1 Site at PlaCe.). Metabolic engineering of natural Omar Borsani products and novel metabolites in plants and it's URUGUAY impact on preexisting pathways as analyzed by metabolite profiling, transcriptome and I integrate the Biochemistry Laboratory of phenotypic analysis. Facultad de Agronomía. These studies included analysis of activity and expression of different enzymes related with nitrogen osmolyte biosynthesis and antioxidant defenses. I study Mónica Susana Barufaldi the plant growth regulator interaction under ARGENTINA deficit water stress through mutants screening analysis. I am using Lotus sp. to analyze the The project “Genetic Improvement of an ABA participation on water-oxidative stress induced tetraploid population of Lotus glaber plant response specifically we are interested in Mill. (=Lotus tenuis)” is based at the Faculty of the study of ABA perception and signaling Agronomy, National University of the Centre of pathways ABA dependent. the Province of Buenos Aires, supported by funds provided by the Secretariat of Science and Technology of the University. A colchicine- induced autotetraploid population produced by Miguel Cauhépé the Faculty of Agronomy in Azul was generated ARGENTINA with the objective of carrying out genetic, cytogenetical, physiological, microbiological I got my undergraduate degree of Agronomic (symbiotic associations), agronomical and Engineer (agronomist) in Universidad del Sur (Bahía Blanca), a MS at Balcarce and the PhD at Lotus activities. 27
Colorado State University in Range Animal Technology of the University. A colchicine- Nutrition. I have done research for INTA since induced autotetraploid population produced by 1967 till 1988, in natural grassland ecology and the Faculty of Agronomy in Azul was generated management. I have published papers in with the objective of carrying out genetic, Argentine, Chile, Brazil and Spain and also cytogenetical, physiological, microbiological some in the Journal of Range Management in (symbiotic associations) and biochemical USA. My latest research deals with fire studies. Furthermore, this germoplasm will be management in tussock grasslands of the humid evaluated with the aim of obtaining an improved Pampas. Lately, I was professor at the population that contemplates the possibility of University of Mar del Plata where with an obtaining tetraploid cultivars, selected for the undergraduate course of Introduction to pastureland conditions of the Depressed Pampa Agronomy and a graduate course in Rangeland of the Province of Buenos Aires. My particular Ecology and Management. I also teach role will be to evaluate the genetic improvement graduates courses on Scientific Writing and in phosphorus and nitrogen efficiency during the Research Methodology at the Graduate School selection by biomass production in soil with on Crop Production at Balcarce. I also did high and low phosphorus availability. consultant work in Nicaragua and Uruguay. I have many activities as an external consultant and peer reviewer on several Argentina’s Universities, CONICET, CONEAU, etc. I am Quentin Cronk chairman of the Agronomy Committee of the CANADA Buenos Aires Commission of Scientific Working on florally expressed genes in Lotus Research (CIC). As a private consultant I am and other legumes, gene phylogenies in Lotus assisting some cow-calf ranches in the Buenos species, see: Citerne HL, Luo D, Pennington Aires Province and also an on farm research on RT, Coen E, Cronk QC. 2003 A phylogenomic natural grasslands productivity. investigation of CYCLOIDEA-like TCP genes in the Leguminosae. Plant Physiology, 131, 1042-53.
Maurizio Chiurazzi ITALY New Agrobacterium tumefaciens mediated in vitro transformation-regeneration procedures. T- DNA tagging program to identify genes Hilda Nélida Crosta involved in Symbiotic Nitrogen Fixation. ARGENTINA Isolation and characterization of high affinity Current research: The project “Genetic ammonium transporter genes; analysis of their Improvement of an induced tetraploid role during SNF. population of Lotus glaber Mill. (=Lotus tenuis)” is based at the Faculty of Agronomy, National University of the Centre of the Province of Buenos Aires, supported by funds Daniel H. Cogliatti provided by the Secretariat of Science and ARGENTINA Technology of the University. A colchicine- The project “Genetic Improvement of an induced autotetraploid population produced by induced tetraploid population of Lotus glaber the Faculty of Agronomy in Azul was generated Mill. (=Lotus tenuis)” is based at the Faculty of with the objective of carrying out genetic, Agronomy, National University of the Centre of cytogenetical, physiological, microbiological the Province of Buenos Aires, supported by (symbiotic associations), agronomical and funds provided by the Secretariat of Science and biochemical studies. Furthermore, this 28 Lotus activities. germplasm will be evaluated with the aim of obtaining an improved population that Jose Pedro De Battista contemplates the possibility of obtaining ARGENTINA tetraploid cultivars, selected for the pastureland Agronomist from La Plata University conditions of the Depressed Pampa of the (Argentina) and MSc. from University of Province of Buenos Aires. My purpose is to verify, by mitotic cromosome counts, the Georgia (USA). Forage researcher at INTA stability of the ploidy level of tetraploid (Argentina) since 1983. Actual research: forage breeding in Lolium multiflorum and Bromus germplasm from different cycles of auleticus, forage germplasm evaluation (Lotus, multiplication. Trifolium, Medicago, Lolium, Festuca), pasture
fertilization and management.
Francesco Damiani ITALY Guilhem Desbrosses FRANCE I started working on Lotus as model system to investigate the possibility to exploit somaclonal We are interested in understanding the various variation in breeding of forage species. developmental programs that occurs in Lotus root (lateral root and nodule) at the same time. Afterward because of my interest was oriented We would like to understand how (identification towards the exploitation of novel techniques of the genes involved) the plant is able to (protoplast fusion and genetic transformation) Lotus was selected as model system to apply coordinate these various developmental such methods. At this purpose methods of tissue programs. In parallel we are establishing protocol to perform Lotus japonicus metabolite culture and plant regeneration, protoplast profiling in order to characterize the metabolism isolation and plant trasformation were occurring in the various plant organs (flower, developed in several Lotus species leaves, nodule…). This will be use later to (L.corniculatus, L.glaber, L.angustissimus, L.uliginosus). At the moment I am studying in characterize the metabolism of various Lotus Lotus the genetics of flavonoid pathway with mutant impaired in nitrogen fixation or other processes. Finally we are identifying using particular interest on the identification of molecular strategies, the genes playing a role in regulatory and structural genes responsible of the early response of Arabidopsis thaliana to a the synthesis of condensed tannins. At his purpose we have produced, through genetic Plant Growth Promoting Rhizobacteria (PGPR). transformation, several polymorphic individuals We are interested in testing whether these genes are also involved in the response of legumes to which accumulate, alternatively, elevated or null Rhizobium inoculation. Using these data, we levels of condensed tannins. On such plants, would like then to describe how the function of experiments consisting on the analysis of gene genes involved in plant microbe interaction is expression under different environmental conditions are performed. Such materials were conserved across phylum. utilized for isolating differentially expressed genes putatively committed with the pathway.
Several Lotus genes committed with the Pedro Díaz Gadea flavonoid pathway have been cloned. URUGUAY
I integrate the Biochemistry Laboratory of Facultad de Agronomía. I work in Nitrogen metabolism (nitrate and ammonium assimilation) in plants under abiotic stress using Lotus activities. 29 agronomical valuable lotus species and its population spread of L.glaber affected by cattle model species Lotus japonicus, specifically in grazing. the response to water deficit stress. These studies included analysis of activity and expression of different enzymes related with nitrogen osmolyte biosynthesis and antioxidant Henk Franssen defenses. I am studying the influence of THE NETHERLANDS Nitrogen nutrition as regulatory mechanism on Research interest is the elucidation of the proline synthesis accumulation under osmotic molecular mechanisms underlying root nodule stress focusing the work specifically on the formation in L.japonicus as an example of organ relation with abscisic acid and light under water development/evolution in plants. To this end we stress conditions. have isolated genes upregulated during symbiosis. One of these genes ENOD40 has an unorthodox structure and the nature of the biological activity residing in this gene is under Marcelo Francisco Eseiza investigation. This gene is also used to ARGENTINA understand how legumes have recruited genes for nodule formation. The project “Genetic Improvement of an induced tetraploid population of Lotus glaber Mill. (=Lotus tenuis)” is based at the Faculty of Agronomy, National University of the Centre Gabino Garcia de Los Santos of the Province of Buenos Aires, supported by MEXICO funds provided by the Secretariat of Science and Technology of the University. A colchicine- Research responsibilities are in temperate crops induced autotetraploid population produced by such as Medicago and Lotus spp. Most the Faculty of Agronomy in Azul was generated significant contributions has been the with the objective of carrying out genetic, germplasm characterization of both species, cytogenetical, physiological, microbiological applying agronomic, morphological and (symbiotic associations), agronomical and molecular descriptors. Efforts have also been biochemical studies. Furthermore, this directed toward the identification of outstanding germplasm will be evaluated with the aim of genotypes in seed productivity and quality, and obtaining an improved population that technology generation for seed production in contemplates the possibility of obtaining various other temperate crops. Teaching tetraploid cultivars, selected for the pastureland responsibilities are SEM-606 ( Storage and conditions of the Depressed Pampa of the conservation of seeds), SEM-607 (Introduction Province of Buenos Aires. My purpose is to to genetic resources management) and other verify, by mitotic cromosome counts, the independent study and seminars courses. stability of the ploidy level of tetraploid germplasm from different cycles of multiplication. Alicia Grassano ARGENTINA Osvaldo Néstor Fernández Lines of Investigation: Biological Fixation of ARGENTINA Nitrogen in Lotus glaber. Bacteria for growth Background research: population dynamics and promotion, with emphasis in phosphorus- biomass production of L.glaber under cattle solubilizing bacteria. grazing. Demography and plant-animal interactions. Current research: persistance and 30 Lotus activities.
Peter Gresshoff Kyung-Nam Kim AUSTRALIA KOREA Our Lotus research is in the following areas: 1) I have received my Ph.D in 1997 from the Characterisation of T-DNA promoter-less GUS Intercollegiate Graduate Program in Plant lines. We have transferred Gifu with Physiology at the Pennsylvania State University, Agrobacterium tumefaciens carrying a USA. The title of my Ph.D. thesis was promoter-less GUS gene. Lines that express “Molecular Analysis of Starch Branching GUS were isolated and characterised. Key lines Enzyme Genes in Maize (Zea mays L.)”. In are: “Fata Morgana” (expression in developing 1998, I joined Dr. Sheng Luan’s lab in nodules, closely linked to ENOD40); “Vasco” University of California at Berkeley, USA, as (expression in nodule vascular bundle); and post-doctoral scholar to study calcium-signaling “Cheetah” (expression in root and nodule pathways in Arabidopsis thaliana, which are meristems). In collaboration with the Kazusa mediated by calcineurin B-like calcium sensors Institute the region surrounding DNA regions (CBLs). Since the year 2001 in which I got the have been characterized. 2) Isolation and faculty position in Sejong University, I have characterisation of early nodulation genes. been interested in working on Lotus. Although Chemical and fast-neutron mutagenesis were my current research topics mainly focus on used to isolate non-nodulating and low elucidating stress-signal transduction pathways nodulating lines of Gifu. Additionally non- in Arabidopsis, I’d like to expand my interests nodulating variants were derived from cell and research to Lotus. cultures. This includes line “Abacus”, characterized by temperature-sensitive nodulation. 3) Analysis of phytohormone perception: mutants and transgenics in key Tatiana E. Kramina phytohormone perception pathways are being RUSSIA investigated for developmental alterations. 4) My first scientific study was devoted to the Analysis of gene networks: mutants and taxonomy of Lotus corniculatus complex. My transgenics altered in defined developmental recent research is aimed in taxonomic revision steps are combined to determine gene of the section Lotus: Lotus alpinus Schleicher ex interactions. Ramond, L. angustissimus L., L. armeniacus Kit Tan & Sorger, L. borbasii Ujhelyi, L. burttii Borsos, L. castellanus Boiss. et Reut. ex Boiss,
L. corniculatus L., L. decumbens Poir., L. Qunyi Jiang degenii Ujhelyi, L. delortii Timb.-Lagr. ex AUSTRALIA F.W.Schultz, L. filicaulis Dur., L. glacialis Nodulation and root development of Lotus (Boiss.) Pau, L. glareosus Boiss. & Reuter, L. japonicus: Gene discovery and gene-gene granadensis Chrtkova-Zertova, L. japonicus interactions. To characterise induced mutant (Regel) K.Larsen, L. krylovii Schischk. & Serg., lines, which are from EMS, Fast Neutron and L. macrotrichus Boiss., L. orphanidis Ujhelyi, insertional mutagenesis and develop an L. palustris Willd., L. parviflorus Desf., L. integrated nodule developmental model based peczoricus Miniaev et Ulle, L. pedunculatus on gene interactions of autoregulation, non- Cav., L. preslii Ten., L. rechingeri Chrtkova- nodulation, hormone-insensitive and promoter- Zertova, L. schoelleri Schweinf., L. stenodon trapped lines. (Boiss. & Heldr.) Heldr., L. stepposus Kramina, L. subbiflorus Lag. (L. suaveolens Pers.), L. glaber Mill (=L. tenuis Waldst. & Kit. ex Willd.), L. ucrainicus Klok., L. uliginosus Schkuhr. Together with Dr. D.D.Sokoloff I am Lotus activities. 31 working at the improvement of the system of the Microbe Interactions 15:368-375) indicating the genus Lotus. relevant polysaccharides role also in this particular interaction. Recently we found that mutants affected in the synthesis of the O- antigen LPS presented a normal nodulation Pedro Laterra development but were out-competed when ARGENTINA coinoculated on Lotus plants with the wild-type My research is aimed to understand population strain. On the other hand, the mutation in the and community processes controlling the periplasmic β (1-2) cyclic glucan synthesis biodiversity and forage value of natural and causes a total impairment of the developing semi-natural grasslands. My study sites are nodule invasion. These studies were made by located in the Flooding Pampa of Argentina, visualization, under the microscope of where L. glaber is widespread. My interest in L. fluorescence, of the infection threads and nodule glaber is focused on the effect of fire regimes on invasion by bacteria labeled with the green its population dynamics, its competitive fluorescence protein (manuscript submitted to relationships with other opportunistic species publication). Now, in collaboration with Dr. M. which colonize burned grasslands, and the Udvardi (Golm, Germany) we are making a allelopathic properties of its seed leachates. comparative study of the plant transcriptome Currently, I am working with two smart students variation front the inoculation with the M. loti on constraints to seedling recruitment associated wild-type and the different mutant strains in to patchiness and fragmentation patterns. order to better understand the signal interchange between both partners.
Viviana Lepek ARGENTINA Lina A.C. Lett ARGENTINA We are interesting in the study of the molecular mechanism that controls the Mesorhizobium The project “Genetic Improvement of an loti- Lotus spp. symbiosis with the final induced tetraploid population of Lotus glaber objective of its application to an improvement of Mill. (=Lotus tenuis)” is based at the Faculty of the process efficiency. Rhizobia specifically Agronomy, National University of the Centre of interact with legume roots inducing structures the Province of Buenos Aires, supported by called nodules where the atmospheric nitrogen funds provided by the Secretariat of Science and fixation occurs. Nodule formation is the result Technology of the University. A colchicine- of a complex signal interchange between the induced autotetraploid population produced by plant host and the bacteria. We have been study the Faculty of Agronomy in Azul was generated the role of different M. loti polysaccharides in with the objective of carrying out genetic, the nodulation process, throughout the isolation cytogenetical, physiological, microbiological of mutants in their synthesis and the (symbiotic associations) and biochemical determination of their nodulation capacity on studies. Furthermore, this germplasm will be Lotus glaber. M. loti Pgm mutant, a mutant evaluated with the aim of obtaining an improved affected in the phosphoglucomutase enzyme, population that contemplates the possibility of was unable to form nodules (Lepek, V. C; obtaining tetraploid cultivars, selected for the D’Antuono, A. L.; Tomatis, P. E.; Ugalde, J. E.; pastureland conditions of the Depressed Pampa Giambiagi, S., and Ugalde, R., A. (2002) of the Province of Buenos Aires. My role will Analysis of Mesorhizobium loti glycogen be to evaluate symbiotic performance and N- operon: effect of phosphoglucomutase (pgm) nutrition of the autotetraploid population under and glycogen synthase (glgA) null mutants on laboratory and greenhouse conditions. nodulation of Lotus tenuis. Molecular Plant- 32 Lotus activities.
concerning enzyme purification and Dasharath Prasad Lohar characterization, molecular biology and USA physiology of key enzymes of N-assimilation such as nitrate and nitrite reductases, glutamine I did my BSc, Agriculture (plant breeding) in synthetases and glutamate synthases, as well as Nepal, and worked as a horticulturist. I the isolation and characterization of mutants completed my MSc (Applied Plant Sciences) in affected in the process. 1994 from the University of London (Wye
College), and thereafter worked for Nepal Agricultural Research Council as a Senior Horticulturist. I started working on Lotus Jim H. McAdam japonicus in 1997. I worked on promoter NORTHERN IRELAND trapping and insertional mutagenesis in Dr. Peter Gresshoff lab (University of Tennessee, I am interested in the role of legumes in upland Knoxville). While there, I developed L. pasture in cool temperate regions. In Northern japonicus transformation technique using bar Ireland there is a need for legumes to be gene as a selectable marker that reduces tissue introduced into cool, wet upland areas with acid culture induced sterility. I also over-expressed soils. I provide scientific advice to the etr1-1 allele in L. japonicus constructing agronomy programme of the Department of transgenic plants that were resistant to ethylene, Agriculture in the Falkland Islands where the and hypernodulated by M. loti. The need is for a Lotus species to tolerate low pH, hypernodulation was dependent on the level of dry, cold soils, particularly within an organic ethylene insensitivity, and was sensitive to scenario. nitrate in the growth medium. The later part of the research was completed in Dr. Gary Stacey lab (UT, Knoxville) where I completed my PhD in 2001. I worked as a research associate in Dr. Jorge Monza David Bird’s lab (North Carolina State URUGUAY University) where I optimized the conditions for I integrate the Biochemistry Laboratory of the study of root-parasitic nematodes in L. Facultad de Agronomía. I work in two main japonicus, and discovered that ecotype Gifu is research lines: abiotic stress and Nitrogen resistant to soybean cyst nematode (Heterodera metabolism of Lotus sp. and biochemistry- glycine). Currently, I am working on transcript genetic identification of native rhizobia profiling in Medicago truncatula, and studying nodulating lotus. Abiotic stress studies are the biology of genes identified during the focused on agronomical valuable lotus species exercise in Dr. Kathryn VandenBosch lab and its model species Lotus japonicus, (University of Minnesota, St. Paul). L. japonicus specifically in the response to water deficit is still my plant of interest, and I would like to stress. These studies included analysis of utilize it in comparison with M. truncatula for activity and expression of different enzymes symbiosis and plant development studies. related with Nitrogen osmolyte biosynthesis and antioxidant defences. I have experience in lotus Nitrogen metabolism and biochemical stress responses in plants and I have worked in genetic Antonio J. Márquez manipulation of rhizobia and their molecular SPAIN and biochemical characterization. The group of Dr. Antonio J. Márquez at the
Plant Biochemistry and Molecular Biology
Department, University of Seville (Spain), is a group specialized on plant nitrogen metabolism and has carried out different types of work Lotus activities. 33
Takuji Ohyama Alicia Orea JAPAN SPAIN We have been studying on Nitrogen fixation and We have studied growth and nitrate assimilation Nitrogen metabolism of soybean plants using in Lotus japonicus plants under different tracer technique with 15N, 13N, 14C, 11C. Now nitrogen sources, as well as the influence of our laboratory group is concentrated on the plant age and growth conditions on nitrate autoreguratory control of nodulation using assimilation (Pajuelo et al., 2002). A full-length hypernodulating mutant lines and parent of cDNA root ferrodoxin-nitrite reductase (NiR) soybean. Also we are interested in the inhibitory has been cloned and characterized. The effect of nitrate on nodule growth and Nitrogen expression of NiR has been studied in different fixation activity. Recently we began to study on tissues and culture conditions (Orea et al., the above point using Lotus japonicus plants and 2001). A mutagenesis programme has been the macro array analysis. I think Lotus is a good performed using ethyl methanesulphate (EMS) model leguminous plant to study Nitrogen on Lotus japonicus in order to isolate nitrate fixation and metabolism as well as genetic assimilation and photorespiratory mutants. Two modification. chlorate resistant mutants (Ljchl1 and Ljchl2) and three photorespiratory mutants (Ljpr1, Ljpr2 and Ljpr3) were isolated. Photorespiratory mutants showed low levels of glutamine Fernando Olmos synthetase (GS) activity, being specifically URUGUAY affected in plastidic GS isoform (Orea et al., 2002). We are currently characterizing these From 1982 to 1992 Lotus corniculatus was used mutants. in mixtures with white clover and grasses as improved pastures in agricultural systems research. The pre-harvest sowing of forage mixtures technique was developed for soybean, corn, sunflower and corn. Farmers adopted it by María Cristina de Pablo using planes. Since 1992 to present Lotus ARGENTINA corniculatus, Lotus subiflorus and Lotus The project “Genetic Improvement of an uliginosus were used among other forage induced tetraploid population of Lotus glaber legumes, to improve natural grasslands (quality Mill. (=Lotus tenuis)” is based at the Faculty of and productivity) by oversowing. Research has Agronomy, National University of the Centre of been focused on main factors affecting the Province of Buenos Aires, supported by population dynamics of lotus in improved funds provided by the Secretariat of Science and grasslands: initial density, annual application of Technology of the University. A colchicine- phosphates, seed production (Olmos, F. 2001. induced autotetraploid population produced by Mejoramiento de pasturas con lotus en la región the Faculty of Agronomy in Azul was generated noreste. Serie Técnica INIA Tacuarembó No. with the objective of carrying out genetic, 124, 48 p. [Improvement of natural grasslands cytogenetical, physiological, microbiological with trefoil in the Northeastern region [in (symbiotic associations), agronomical and Spanish]]). Present: assessment of proposed biochemical studies. Furthermore, this management practices with grazing animals. germplasm will be evaluated with the aim of obtaining an improved population that contemplates the possibility of obtaining tetraploid cultivars, selected for the pastureland conditions of the Depressed Pampa of the Province of Buenos Aires. My role will be to 34 Lotus activities. evaluate seed quality of tetraploid germplasm from different multiplication cycles. J.S. Grant Reid UNITED KINGDOM
Peter Paľove-Balang Our primary interest is in the Golgi membrane- SLOVAK REPUBLIC bound enzymes of plant cell wall polysaccharide biosynthesis, but we are using Lotus japonicus Nitrogen metabolism of different plants (maize, to investigate the effect on galactomannan barley, Lotus), mainly the regulation of uptake structure in Lotus seed endosperm of and reduction of nitrogen under normal and downregulating Galactomannan galactosyl- stress conditions. Actual research: transferase. We have a manuscript in Plant Characterization of chlorate-resistant mutants Physiology (in press). of Lotus japonicus, Cd-stress in maize.
William John Rogers Martin Parniske ARGENTINA ENGLAND Current research: The project “Genetic My background is the molecular and genetic Improvement of an induced tetraploid analysis of plant microbe interactions. The focus population of Lotus glaber Mill. (=Lotus of my laboratory is the genetics of plant root tenuis)” is based at the Faculty of Agronomy, symbiosis and we are using Lotus japonicus as National University of the Centre of the genetic model organism. We have developed Province of Buenos Aires, supported by funds large populations of EMS mutagenised seed and provided by the Secretariat of Science and have isolated several hundred mutants affected Technology of the University. A colchicine- in root symbiosis. We also have established a induced autotetraploid population produced by TILLING reverse genetics tool for Lotus the Faculty of Agronomy in Azul was generated japonicus, which is accessible for other with the objective of carrying out genetic, laboratories through collaboration. cytogenetical, physiological, microbiological (symbiotic associations), agronomical and biochemical studies. Furthermore, this germplasm will be evaluated with the aim of Mónica Rebuffo obtaining an improved population that URUGUAY contemplates the possibility of obtaining tetraploid cultivars, selected for the pastureland I integrate the Animal Production Area of the conditions of the Depressed Pampa of the National Institute of Agricultural Research since Province of Buenos Aires. My particular role 1979. My background is in plant breeding and will be to evaluate the genotypic stability of the agronomy. The focus of my research has been autotetraploid population through the development of new varieties of Lotus electrophoretic analysis of individual seeds of corniculatus, L.uliginosus and L.subbifloru for plants selected for thousand grain weight from Uruguayan farmers. We have developed different cycles of multiplication. birdsfoot trefoil cv. INIA Draco, with improved persistence and forage production, whereas big trefoil experimental line LE 627 has been selected for forage and seed production. My Oscar Adolfo Ruiz latest research deals with the joint breeding ARGENTINA program with Dr. P.Beuselinck, aimed to introgress rhizomes into adapted germplasm. IIB-INTECH group is working in the study of physiological aspects of the response of Lotus Lotus activities. 35 spp to abiotic stresses. Emphasis will be directed to the effects of salinity, an important Juan Sanjuán constraint for the propagation of Lotus spp. in SPAIN the lowlands of the Salado River Basin, a My previous experience with Lotus symbionts is 9,000,000 ha region located in Buenos Aires limited. I currently have a research grant that Province (Argentina) representing the most important area devoted to beef cattle production includes work on the genetic relationships in this country. L. glaber has been introduced in between nitrogen-fixing efficiency and osmotic stress tolerance of Lotus rhizobia. Also, this region around 1930 and has successfully determining the genetic diversity of Lotus established, showing a high potential for the glaber rhizobia from the Salado River Basin in colonization of saline lowlands. However, a strong variability in salt tolerance is found Argentina is part of our immediate research. among genotypes belonging to natural populations, probably as a result of being a strict cross-pollinated species. Thus, physiological Toshiki Uchiumi studies are going to be conducted using extreme genotypes of L. glaber. Also we are JAPAN participating in the collection of germplasm Expression analysis of whole genome of from different environments within the above Mesorhizobium loti under symbiosis with Lotus mentioned region and in the collection and japonicus. The role of nonsymbiotic identification of rhizobial and mycorhizal hemoglobin on the interaction between symbionts from Lotus plants growing in diverse microsymbionts and L. japonicus. environments within the Salado River region. Moreover, we are working in the molecular and physiological characterization of a registered cultivar of L. glaber (INTA-PAMPA), which Osvaldo Ramón Vignolio has been obtained by recurrent selection for high ARGENTINA yield under saline conditions typical of the We studied the flooding tolerance of Lotus Salado River Basin. glaber and Lotus corniculatus plants and seed. Flooding tolerance was studied in plants of different age or size and populations. Biomass allocation to vegetative and reproductive organs in Lotus glaber and L. corniculatus also was Federico Sanchez studied. Besides, seed germination of five MÉXICO populations of L. glaber under saline conditions and in different soil was studied. Effects of L. Plant cytoskeleton and cell signaling during the glaber density upon the emergence, survival and Rhizobium-legume symbiotic interaction. In cover of a weed (Cirsium vulgare) that grows in particular, I work with actin, profilin and other grassland of Paspalum quadrifarium after actin-binding proteins and with proteins from burning, were evaluated. Now we are interesting root-nodules with a particular role during the in knowing the compensatory responses of L. development of this organ. glaber to defoliation, biomass and seed production, and seed survival to cattle digestion. As well as, the survival of seedling growing in cow dug.
36 Lotus activities.
IGER has access to a range of unique plants, Jeffrey J. Volenec including symbiotic mutants, transformants with USA altered gene expression, and genetically tagged transformants. This research will advance our The mission of our research is to identify and understanding of genes involved in several characterize physiological and biochemical interactions between legumes and mechanisms influencing growth and stress microorganisms. Such information will help tolerance of forage legumes, primarily alfalfa legume breeders develop precision breeding (but these questions have relevance to trefoil as programmes for agronomically important well). Four research topics are currently being species. examined in detail. 1) Characterize mechanisms controlling synthesis and degradation of organic reserves (starches, sugars, and storage proteins) in legume roots, and understand the role of organic reserves in shoot growth and stress tolerance. 2) Understand physiological and molecular factors controlling crown bud dormancy and bud development, and their impact on shoot growth. 3) Determine the physiological and molecular mechanisms controlling fall dormancy, and how these impact winter hardiness and growth of alfalfa. 4) Understand how potassium and phosphate nutrition alters physiological and biochemical processes in alfalfa roots that ultimately improve alfalfa persistence and growth.
Judith Webb WALES, UK My research is aimed at understanding genetic interactions between legumes and organisms that affect plant health and performance. These studies focus on the beneficial organisms, Rhizobium bacteria and arbuscular mycorrhiza fungi. Rhizobium fixes atmospheric Nitrogen in the root nodules of legumes such as white clover, an important UK forage species. Nitrogen fixing forages are key features of low input farming systems, offering high quality food for herbivores and increasing the level of nitrogen in the soil-plant-animal system. Symbiotic arbuscular mycorrhizae - which are not restricted to legumes - supply their plant hosts with phosphate and may protect them from attack by certain pathogens. Part of my research centres on the model legume Lotus japonicus, which is an ideal candidate for genetic analysis. Lotus Newsletter (2003) Volume 33, 37 – 62.
Current list of Lotus researchers
Database last updated Nov. 30 2003
Hernan Acuña Canelones CP 90200 Director Uruguay Centro Regional de Investigación Quilamapu, [email protected] INIA http://www.inia.org.uy Casilla 426 Phone: +598-2-3677641 Chillan Fax No.: +598-2-3677609 Chile L.corniculatus, L.subbiflorus, L.pedunculatus. [email protected] Pathology. Phone: 56-42-211177 entry last revised Nov 7 2003 Fax No.: 56-42-217852 L.corniculatus, L.glaber, L.uliginosus. Breeding, utilization, germplasm, seed, Rosario Alzugaray reclamation, physiology, forage. Germination, Researcher emergence, establishment and vegetative INIA Uruguay growth of Lotus sp. in clay soils. Plant Protection entry last revised Nov 7 2003 CC 39173 Colonia Uruguay [email protected] Kenneth A. Albrecht http://www.inia.org.uy Professor Phone: +598-574-8000 ext 1464 Univ. of Wisconsin-Madison Fax No.: +598-574-8012 Department of Agronomy L.corniculatus. Entomology. 1575 Linden Dr. entry last revised Oct 29 2003 Madison WI 53706 U.S.A. [email protected] Said Amrani Phone: +1-608-262-2314 Laboratorie de Biologie du Sol Fax No.: +1-608-262-5217 Institut des Sciences de la Nature L. corniculatus; L. uliginosus. Ecology; forage USTHB - BP 32 El Alia - Bab Ezzouar production; utilization. 16111 – Alger entry last revised Oct 23 2003 Algeria [email protected] Phone: +(02) 24-72-17 Nora Altier Taxonomy; seed; utilization; biotechnology; Researcher Rhizobium-legumes symbiosis; biological INIA, Nacional Institute for Agricultural nitrogen fixation. Survey of nodulation and Research nirogen fixation among legumes of Algeria. Department of Plant Pathology Phylogenetic aspects and coevolution threads INIA Las Brujas of the two symbionts. Ruta 48 km.10
37
38 Lotus researchers.
Toshio Aoki Ariel Asuaga Assistant Professor Ingeniero Agrónomo Nihom University Lancasteriana 2284 College of Bioresource Sciences Montevideo Department of Applied Biological Sciences Uruguay Kameino 1866 [email protected] Fujisawa Phone: +598-2-9160279; +598-2-6007821 Kanagawa 252-8510 Fax No.: +598-2-9162881 Japan L.corniculatus, L.glaber, L. uliginosus, L. [email protected] subbiflorus. Genetics, Breeding, Germplasm, Phone: +81-466-843703 Physiology, Forage production, Seed Fax.no.: +81-466-843353 production, Pathology. L.japonicus. Genetics, Physiology, Tissue entry last revised Nov 4 2003 Culture, Molecular Biology entry last revised Oct 13 2003
John F. Ayres Ana Arambarri Supervisor of Research & Principal Research Profesora Scientist Facultad de Ciencias Agrarias y Forestales Agricultural Research & Advisory Station, Universidad Nacional del La Plata ‘Centre for Perennial Grazing Systems’ Calles 60 y 118 - C.C. 31 NSW Agriculture C. P. 1900 La Plata PMB Glen Innes Prov. Buenos Aires New South Wales 2370 Argentina Australia [email protected] [email protected] Phone: +54-221-423-6618 Phone: +61-2-67301930 Fax No.: +54-221-425-2346 Fax No.: +61-2-67301999 Lotus species; Acmispon, Hosackia and L. uliginosus, L. corniculatus. Breeding, Syrmatium. Plant taxonomy; seeds. Old and Ecology, Forage Production, Utilization. New World Lotus species; epidermal entry last revised Nov 18 2003 characteristics. Taxonomy of the New World species, known as: Acmispon, Hosackia and Syrmatium. entry last revised Oct 17 2003 Andreas Bachmair Group leader Max Planck Institute for Plant Breeding Alberto Artola Research Breeder Plant Developmental Biology IPB Semillas Carl-von-Linné-Weg 10 Crop Department D-50829 Cologne Enrique Hurtado Nº 11 Germany Colonia del Sacramento. CP 70.000 [email protected] Uruguay http://www.mpiz-koeln.mpg.de/ [email protected] Phone: +49-221-5062265/266 Phone: +598-52-23742 Fax No.: +49-221-5062207 L.corniculatus. Seed Production, Crop L.japonicus (currently no research activity with Establishment. Development of seed vigor tests Lotus). Genome organization, retrotransposons. and seed enhancement methods. entry last revised Oct 30 2003 entry last revised Nov 24 2003
Lotus researchers. 39
Argentina Søren Bak [email protected] Associate Professor http://www.faa.unicen.edu.ar/ The Royal Veterinary and Agricultural Phone: +54-2281-433291/92/93 University, Department Plant Biology Fax No.: +54-2281-43329/92/93 DK-1871Thorvaldsensvej 40 L.glaber; L.corniculatus. Genetics; breeding; Frederiksberg C, Copenhagen forage production; seed production. Generate, Denmark verify, and evaluate autotetraploid (via [email protected] colchicine) populations of L.glaber. http://www.biobase.dk/P450 entry last revised Oct 31 2003 http://plbio.kvl.dk http://www.place.kvl.dk Phone: +45-35283346 Fax No.: +45-38283333 Manuel Becana L.japonicus. Metabolite pathways. Metabolite Professor profiling, transcriptome. Estacion Experimental de Aula Dei, CSIC entry last revised Oct 16 2003 Department Plant Nutrition Avda Montañana 1005, Apdo 202 50080 Zaragoza P.A. Balatti Spain CERLAP-CONICET [email protected] Buenos Aires Phone: +34-976-716055 Argentina Fax No.: +34-976-716145 [email protected] L.japonicus. Biochemistry, Molecular Biology. Fax No: 54-21-530189 Antioxidants –Abiotic stress (drought, salinity, L. glaber; L. corniculatus. Genetics; pathology. heavy metals)- Free radicals-Thiol metabolism- Genetics of nitrogen-fixing symbiots. Nodule senescence-Oxidative stress. entry last revised Oct 16 2003
Gary S. Bañuelos USDA-ARS D. P. Belesky Water Management Research Laboratory USDA-ARS 9611 S. Riverbend Ave. Appalachian Soil & Water Conservation Lab Parlier CA 93648 P.O. Box 867 U.S.A. Airport Road [email protected] Beckley, WV 25801-0867 Phone: +1-559-596-2880 U.S.A. Fax No.: +1-559-596-2851 [email protected] L. corniculatus; L. glaber. Remediation of Phone: 304-256-2841 trace element-laden soils with plants. Fax No.: 304-256-2921 entry last revised Oct 13 2003 L.corniculatus. Ecology; forage; utilization. Lotus use in low-input pastures and on marginal soil/landscapes. Mónica Susana Barufaldi Ayudante graduado Universidad Nacional del Centro de la María Bemhaja Provincia de Buenos Aires (UNCPBA) Forage Researcher Facultad de Agronomía INIA Uruguay Ciencias Básicas Agronómicas y Biológicas Forage Department, Animal Production Av. República Italia 780, C.C. 47 R. 5 Km 386 (7300) Azul, Provincia de Buenos Aires Tacuarembó 45000
40 Lotus researchers.
Uruguay Phone: +1-605-688-4759 [email protected] Fax No.: +1-605-688-4452 http://www.inia.org.uy L. corniculatus; L. purshianus. Genetics; Phone: +598-632-4560 breeding; seed production; entomology. Fax No.: +598-632-3969 entry last revised Nov 6 2003 L.corniculatus, L.uliginosus. Physiology, Forage and seed production, Utilization. entry last revised Nov 5 2003 Søren Borg Forskningscenter Flakkebjerg Flakkebjerg David Bertioli DK-4200 Slagelse PBI Denmark EMBRAPA Recursos Genéticos e [email protected] Biotecnologia Parque Estação Biológica-pqEB, Final Av. W5 Norte Omar Borsani Brasília-DF Assistant Professor CEP: 70770-900 Facultad de Agronomía Brazil Departamento de Biología Vegetal, Bioquímica [email protected] Av. Garzón 780, Montevideo entry last revised Nov 20 2003 Uruguay [email protected] http://www.fagro.edu.uy/bioquimica Paul R. Beuselinck Phone: +598-2-3540229 USDA-ARS Plant Genetics Research Unit Fax No.: ++ 598-2-3543004 University of Missouri L.corniculatus, L.uliginosus, L.japonicus. 207 Waters Hall Biology, Physiology, Tissue Culture, Columbia, MO 65211 Molecular Biology. U.S.A. entry last revised Nov 18 2003 [email protected] Phone: +1-314-268-3114 Fax No.: +1-314-882-1467 Caroline Bowsher L.corniculatus, L.glaber, L.uliginosus, Dept. of Cell and Structural Biology L.japonicus. Formerly Lotus spp. genetics; School of Biological Sciences breeding; germplasm. Current research University of Manchester emphasis is on soybean seed composition and Williamson Bd. Oxford Rd. seed physiology. Former research was on Lotus Manchester M13 9PL breeding and selection for improved UK persistence and evaluation of exotic [email protected] germplasm. entry last revised Nov 7 2003 E. Charlie Brummer Associate Professor Arvid A. Boe Iowa State University Professor of Plant Science 1204 Agronomy Hall Plant Science Department Ames Iowa 50011 South Dakota State University U.S.A. NPB 244A, Box 2140C [email protected] Brookings, SD 57007 http://www.public.iastate.edu/~brummer U.S.A. Phone: +1-515-294-1415 [email protected]
Lotus researchers. 41
Fax No. : +1-515-294-6505 L.corniculatus. Genetics; breeding; forage; Bernard J. Carroll utilization; ecology. Max-Planck-Institut fur Molekulare entry last revised Oct 23 2003 Pflanzenphysiologie Karl-Liebknecht-Str. 24-25 14476 Golm Joe Brummer Germany Colorado State University Mountain Meadow Research Center P.O. Box 598 Fabricio Dario Cassán Gunnison, CO 81230 Posdoctoral, CONICET U.S.A. Laboratorio de Fisiología Vegetal-UNRC [email protected] Laboratorio de Biotecnología 1-IIB-INTECh. Phone: 970/641-2515 Universidad Nacional de Rio Cuarto Fax No.: 970/641-0653 Campus Universitario, Ruta 36, km 601, L. corniculatus; L. wrightii. Genetics; (5800) Rio Cuarto breeding; forage; utilization; ecology. Argentina Improvement of forage production and quality [email protected] in mountain meadows. Phone: +54-358-4676103 Fax No.: +54-358-4676230 L.glaver. Microbiology. Anton van Brussel entry last revised Nov 12 2003 Assistant professor Leiden University Institute of Molecular Plant Sciences Miguel Cauhépé Postbus 9505 Private Researcher and Consultant on Range 2300 RA Leiden and Pasture Management The Netherlands Cereijo 979 [email protected] 7620 Balcarce (Prov. de Buenos Aires) Phone: +31-71-5275068 Argentina Fax No.: +31-71-5275088 [email protected] L.japonicus, L.pressli. Physiology, Phone: +54-266-430668 Microbiology, Molecular Biology (currently no Fax No.: +54-266-430908 research activity with Lotus). Autoregulation of L.glaber. Ecology, Forage Production, nodulation of Vetch (Vicia) and Rhizobium Utilization leguminosarum. entry last revised Nov 3 2003 entry last revised Oct 30 2003
Maurizio Chiurazzi Gustavo Caetano-Anolles Institute of Genetics and Biophysics “A. Division of Botany and Plant Physiology Buzzati Traverso” Department of Biology Via Marconi 12 University of Oslo 80125 Naples PO Box 1045 Blindern Italy N-0316 Oslo [email protected] Norway http://www.iigb.na.cnr.it/ [email protected] Phone: +39-081-7257256 Fax No.: +47 22 85 46 64 Fax.no.: +39-081-5936123 L.japonicus. entry last revised Oct 13 2003
42 Lotus researchers.
Provincia de Buenos Aires (UNCPBA). Daniel H. Cogliatti Ciencias Básicas Agronómicas y Biológicas. Professor of Plant Physiology Av. República Italia 780, C.C. 47. Facultad de Agronomía, Universidad Nacional (7300) Azul, Provincia de Buenos Aires. del Centro de la Provincia de Buenos Aires Argentina Departamento de Ciencias Básicas [email protected] Agronómicas y Biológicas http://www.faa.unicen.edu.ar Av. República Italia 780 C.C. 47, (7300) Azul Phone: +54-2281-433291/92/93 Provincia de Buenos Aires. Fax No.: +54-2281-433291/92/93 Argentina L.glaber; L.corniculatus. Biology, Taxonomy, [email protected] Genetics, Tissue Culture, seed production, http://www.faa.unicen.edu.ar Molecular Biology. Phone: +54-2281-433292 entry last revised Nov 25 2003 Fax No.: +54-2281-433292 L.glaber. Genetics, Physiology, Mineral nutrition. Cristina Cvitanich entry last revised Nov 17 2003 IMB-Lab. of Gene Expression University of Aarhus Science Park Olga Susana Correa Gustav Wieds Vej 10 C Catedra de Microbiologia DK-8000 Aarhus C Departamento de Ecologia Denmark Facultad de Agronomia-UBA Fax.no.:+45 86 12 31 78 Av. San Martin 4453 Argentina [email protected] Miguel Dall'Agnol Faculdade de Agronomia Univ. Federal of Rio Grande do Sul Quentin Cronk Caixa Postal 776 Professor of Plant Science and Director 91501-970 Porto Alegre-RS University of British Columbia Brazil UBC Botanical Garden and Centre for Plant [email protected] Research Phone: +51-3316-7405 6804 SW Marine Drive Fax No.: +51-3316-6045 Vancouver B.C., V6T 1Z4 L.corniculatus; L.uliginosus. Plant Breeding Canada and Genetics. [email protected] entry last revised Oct 8 2003 http://www.ubcbotanicalgarden.org Fax No.: +1-604-822-2016 L.japonicus, L.bertholletii, L.uliginosus, etc Francesco Damiani (including N. American Lotus). Gene Researcher phylogenies, evolution of florally expressed Consiglio Nazionale delle Ricerche genes. Istituto Genetica Vegetale sez Perugia entry last revised Oct 23 2003 via della Madonna Alta 130 06128 Perugia Italy Hilda Nélida Crosta [email protected] Profesor Adjunto http://www.irmgpf.pg.cnr.it Facultad de Agronomía Phone: +39-075-5014862 Universidad Nacional del Centro de la Fax No.: +39-075-5014869
Lotus researchers. 43
L.corniculatus, L.glaber, L.uliginosus, L japonicus. Genetics, Breeding, Molecular Guilhem Desbrosses Biology, Physiology, Tissue Culture. Lecturer entry last revised Oct 10 2003 University of Montpellier II UMR113 / Laboratoire des Symbioses Tropicales et Méditerranéennes / Réponse des David K. Davis plantes aux micro-organismes Superintendent UMR113 / CC002 University of Missouri Agricultural Place Eugène Bataillon Experiment Station F-34095 Forage Systems Research Center and Montpellier Cedex 05 Thompson Farm France 21262 Genoa Rd [email protected] Linneus, MO 64653 Phone: +33-4-67149353 U.S.A. Fax No.: +33-4-67143637 [email protected] L.japonicus. Genetics, Molecular Biology, http://www.aes.missouri.edu/fsrc Microbiology. http://www.aes.missouri.edu/thompson entry last revised Oct 28 2003 Phone: +1-660-895-5121 Fax No.: +1-660-895-5122 L. corniculatus. Physiology; forage; seed. Pedro Díaz Gadea Commercial production and sale of Lotus. Assistant Professor entry last revised Nov 30 2003 Facultad de Agronomía Departamento de Biología Vegetal, Bioquímica Av. Garzón 780, Montevideo Brad Day Uruguay The University of Tennessee [email protected] Department of Microbiology http://www.fagro.edu.uy/bioquimica M409 Walters Life Science Building Phone: +598-2-3540229 Knoxville, Tennessee 37996-0845 Fax No.: +598-2-3543004 U.S.A. L.corniculatus, L.glaber, L.uliginosus, [email protected] L.japonicus, L.filicaulis. Biology, Physiology, Tissue Culture, Molecular Biology. entry last revised Nov 18 2003
José Pedro De Battista Forage researcher Allan Downie INTA EEA Concepción del Uruguay Professor Area de Investigación en Producción Animal John Innes Centre C.C. Nº 6, Concepción del Uruguay Colney Lane (3260) Entre Ríos Norwich NR4 7UH Argentina UK [email protected] [email protected] http://www.inta.gov.ar http://www.jic.ac.uk/staff/allan-downie/ Phone: +54-3442-425561 Phone: +44-1603-450207 Fax No.: +54-3442-425578 Fax No.: +44-1603-450045 L.corniculatus, L.glaber, L.subbiflorus. L.japonicus. Genetics, Molecular Biology, Breeding, Forage production and utilization. Microbiology, Nodulation signalling. entry last revised Nov 12 2003 entry last revised Oct 21 2003
44 Lotus researchers.
Nancy J. Ehlke Manolis Flementakis Department of Agronomy & Plant Genetics Agricultural University of Athens University of Minnesota Botanikos 1991 Buford Circle Iera Odos 75 St. Paul MN 55108 11855 Athens U.S.A. Greece [email protected] [email protected] Phone: +1-612-625-1791 Fax No.: +1-612-625-1268 L. corniculatus. Genetics; breeding. Henk Franssen entry last revised Nov. 30 2003 Assistant Professor Wageningen University Molecular Biology Marcelo Francisco Eseiza Dreijenlaan 3 Jefe de Trabajos Prácticos, Botánica Agrícola I 6703HA Wageningen Facultad de Agronomía, Universidad Nacional The Netherlands del Centro de la Provincia de Buenos Aires [email protected] (UNCPBA) Phone: +31-317-483264 Ciencias Básicas Agronómicas y Biológicas Fax No.: +31-317-483264 Av. República Italia 780, C.C. 47 L.japonicus. Genetics, Molecular Biology, (7300) Azul, Provincia de Buenos Aires developmental program of nodule formation, Argentina nodule evolution. [email protected] entry last revised Oct 24 2003 http://www.faa.unicen.edu.ar Phone: +54-2281-433291 (or 433292 or 433293) Gabino Garcia de Los Santos Fax No.: +54-2281-433291 (or 433292 or Head of Seed Production Department 433293) Instituto de Recursos Genéticos y L.glaber; L.corniculatus. Biology, Genetics, Productividad Tissue Culture, seed. Colegio de Postgraduados. entry last revised Nov 26 2003 Carretera Mexico-Texcoco Km. 36.5 Texcoco EDO. de Mexico 56230 Mexico Osvaldo Néstor Fernández [email protected] Associate Professor Phone: 595 952 02 62 Universidad Nacional de Mar del Plata Fax No.: 34-85-381511 Facultad de Ciencias Agrarias – Group L.corniculatus. Physiology; ecology; breeding; Agroecology taxonomy. Germplasm management, C.C. 276 adaptation, germplasm screening for forage (CP7620) Balcarce and seed production and breeding. Argentina entry last revised Nov 21 2003 [email protected] Phone: +54-2266-439100/05 Fax No.: +54-2266-439105 Kirsten Gausing L.glaber. Population ecology and forage IMSB-University of Aarhus production. C.F. Møllers Alle entry last revised Nov 10 2003 Building 130 DK-8000 Aarhus C
Lotus researchers. 45
Denmark [email protected] [email protected] http://www.forage.prosser.wsu.edu Fax.no.: +45 86 19 65 00 Phone: +1-509-7869265 Fax No.: +1-509-7869370 All Lotus spp. Germplasm curator for Lotus William F. Grant collection in USDA National Plant Germplasm Emeritus Professor System. McGill University entry last revised Nov 21 2003 Plant Science MacDonald Campus, McGill University P.O. Box 4000 Peter Gresshoff Ste. Anne de Bellevue Professor/Director 21,111 Lakeshore Rd. The University of Queensland and ARC Centre Quebec H9X 3V9 of Integrative Legume Research Canada ARC Centre of Excellence for Integrative [email protected] Legume Research http://www.agrenv.mcgill.ca/plant/grant.htm John Hines Plant Science Building http://www.eman- The University of Queensland rese.ca/eman/ecotools/botanists/GrantWF.html St Lucia Qld 4067 Phone: +1-514-3987851, EXT. 7863 Australia Fax No.: +1-514-398-7897 [email protected] L.corniculatus; L.glaber; Lotus spp. Genetics; http://www.legumecentre.cilr.uq.edu.au taxonomy; germplasm. Genetics of Lotus, Phone: +61-7-33653550 especially those involved with the evolution of Fax No.: +61-7-33653559 L.corniculatus. L. japonicus. We conduct research into entry last revised Oct 9 2003 Rhizobium-induced nodulation and utilize mutagenesis and transgenics to create the
necessary diversity needed to determine Alicia Grassano physiological and biochemical processes. Prof. Titular, Química Analítica. Specifically we have used promoter trapping, Universidad Nacional de La Pampa transfer of the Arabidopsis ethylene Facultad de Ciencias Exactas y Naturales insensitivity (via AtEtrl-l) and non-nodulation Departamento de Química mutants. Uruguay 151 entry last revised Oct 13 2003 (6300) Santa Rosa, La Pampa Argentina [email protected] Tomas C. Griggs Phone: +54-2954-436787 University of Idaho Fax No.: +54-2954-432535 Department of Plant-Soil and Entomological L.glaber. Microbiology, Ecology. Sciences entry last revised Oct 21 2003 Moscow ID 83844-2339
U.S.A.
[email protected] Stephanie Greene Phone: 208-885-6531 Geneticist/Curator Fax No.: 208-885-7760 USDA-ARS L.corniculatus, L.uliginosus, L.uliginosus. National Temperate Forage Legume Physiology; ecology; forage; utilization. Germplasm Resources Unit Grass/legume mixture relations and 24106 North Bunn Road plant/animal interactions in pastures; forage Prosser, WA 99350 production and quality. U.S.A.
46 Lotus researchers.
Mette Grønlund Thomas Humphrey Post.doc University of Manchester University of Aarhus UK Lab. of Gene Expression Science Park Gustav Wieds Vej 10 C Alberto A. Iglesias DK-8000 Aarhus C Profesor Asociado UNL Denmark Investigador Principal CONICET [email protected] Grupo de Enzimología Molecular Phone: +45-89425008 Bioquímica Básica de Macromoléculas Fax.no.: +45-86123178 Facultad de Bioquímica y Ciencias Biológicas L.japonicus. Genetics, Biology, Physiology, Universidad Nacional del Litoral Pathology, Tissue culture, Molecular biology. Paraje "El Pozo", CC 242 entry last revised Oct 15 2003 S3000ZAA Santa Fe Argentina [email protected] Judith Harrison entry last revised Nov 30 2003 INRA Laboratoire de Metabolisme 78026 Versailles Cedex France Sachiko Isobe [email protected] National Agricultural Research Center for Hokkaido Region Forage Legume Breeding Lab. Nate Hartwig Hitsujigaoka 1 116 ASI Bldg. Toyohira Department of Agronomy Sapporo, 062-8555 Pennsylvania State University Japan University Park, PA 16802 [email protected] U.S.A. http://cryo.naro.affrc.go.jp/sakumotu/mame [email protected] ka/etop.htm L. corniculatus. Forage; utilization; Phone: +81-11-8579272 management. Cover crops, living mulches in L.japonicus. Breeding, Genetics. corn and soybean. entry last revised Oct 17 2003
José A. Herrera-Cervera Euan James Assistant Research Professor Research Scientist Facultad de Ciencias. University of Granada University of Dundee Departamento de Fisiología Vegetal School of Life Sciences Campus de FuenteNueva s/n. 18071 Centre for High Resolution Imaging & Granada Processing(CHIPs) Spain MSI/WTB Complex [email protected] Dundee DD1 5EH Phone: +34-958243382 UK Fax No.: +34-958248995 [email protected] L.japonicus. Genetics, Physiology, Pathology, Phone: +44-1382-344741 Tissue Culture, Molecular Biology, Fax No.: +44-1382-345893 Microbiology. L.corniculatus, L.uliginosus, L.japonicus. entry last revised Oct 14 2003 Physiology, structure (especially of nodules). entry last revised Oct 18 2003
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820 W. First Street Erik Østergaard Jensen Halsey Oregon 97348 Associate Professor U.S.A. University of Aarhus [email protected] Dept. Molecular Biology. Lab. of Gene Phone: 503-369-2251 Expression Fax No.: 503-369-2640 Science Park L.corniculatus. Genetics; breeding; forage; Gustav Wieds Vej 10 C seed; taxonomy. Identification and DK-8000 Aarhus C characterization of cultivars with high yield Denmark potential. [email protected] http://130.225.13.27/~eoj/ Phone: +45-89425014 Bodil Jørgensen L.japonicus. Molecular biology, physiology, Senior scientist organ development. Danish Institute of Agricultural Science entry last revised Nov. 7 2003 Depart. Plant Biology, Biotechnology Group Thorvaldsensvej 40, opg. 8, 2 sal 1871 Frederiksberg C Qunyi Jiang Denmark Laboratory manager [email protected] The University of Queensland Phone: +45-35282578 ARC Centre of Excellence for Integrative Fax No.: +45-35282589 Legume Research L.japonicus. Tissue culture, transformation, John Hines Building Molecular biology, biochemistry. Brisbane entry last revised Nov 25 2003 Qld 4072 Australia [email protected] Priyavadan A. Joshi www.legumecentre.cilr.uq.edu.au The Royal Vetenary and Agricultural Phone: +61-7-33657227 University Fax No.: +61-7-33653556 Department of Plant Biology Plant Physiology L.japonicus. Genetics, genomics and molecular and Anatomy Lab. biology. Thorvaldsensvej 40 entry last revised Nov 21 2003 DK-1871 Frederiksberg C Denmark [email protected] Bjarne Jochimsen IMSB-Lab. of Gene Expression University of Aarhus Norihito Kanamori C.F. Møllers Alle National Food Research Institute Building 130 Food Engineering Division DK-8000 Aarhus C Kannondai 2-1-12 Denmark Tsukuba-city [email protected] Ibaraki 305-8642 Fax.no.: +45-86196500 Japan Changing lab at present Nov 21 2003 [email protected]
Phone: +81-29-8388047
Fax.No.: +81-29-8391552 Stephen W. Johnson L.japonicus. Breeding and Molecular Biology. International Seeds, Inc. entry last revised Nov 5 2003 P.O. Box 168
48 Lotus researchers.
GPO Box 1600 Panagiotis Katinakis Canberra ACT 2601 Professor Australia Agricultural University of Athens [email protected] Botanikos Phone: +61-2-62465083 Iera Odos 75 Fax No.: +61-2-62465255 11855 Athens L.uliginosus, L.corniculatus. Cultivar breeding Greece and genetic analysis of agronomic traits. [email protected] entry last revised Nov 7 2003 Phone: +30-210-5294314 Fax No.: +30-210-5294314 L.japonicus. Molecular Biology, Biochemistry, Kyung-Nam Kim Physiology. Assistant Professor entry last revised Oct 22 2003 Sejong University Department of Molecular Biology 98 Gunja-Dong Tony Kavanagh Gwangjin-Gu Professor, Head of Department Seoul 143-747 Trinity College Dublin Korea Smurfit Institute [email protected] Department of Genetics Phone: +82-2-34083647 Dublin 2 Fax No.: +82-2-34083661 Ireland Arabidopsis thaliana. L.japonicus. Molecular [email protected] Biology. Phone: +353-1-6081035 entry last revised Nov 20 2003 Fax.no.: +353-1-6714968 L.japonicus. Genetics. entry last revised Oct 14 2003 Joseph H. Kirkbride USDA-Agricultural Research Service Systematic Botany & Mycology Laboratory Masayoshi Kawaguchi Bldg 011A. Rm 304 Associate Professor BARC-West University of Tokyo Beltsville, MD 20707 Graduate School of Sciences U.S.A. Department of Biological Sciences [email protected] Hongo Phone: +1-310-5049447 Bunkyo-ku Fax No.: +1-310-5045435 Tokyo 113-0033 All Lotus spp. Taxonomy. Systematics of Lotus Japan spp. [email protected] entry last revised Oct 9 2003 Fax.no.: +81-3-58414458 L.japonicus, L.burttii. Molecular basis of symbiosis and systemic regulation of nodule Tatiana E. Kramina development Scientific Researcher entry last revised Oct 21 2003 Moscow State University Biological Faculty Higher Plants Department Walter Kelman Vorobyevy Gory, 1, building 12, Research Scientist 199992 Moscow CSIRO Division of Plant Industry Russia Institute of Plant Production and Processing [email protected]
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Phone: +7-095-9391603 Phone: +1-715-2783200 Fax No.: +7-095-9391827 Fax No.: +1-715-2783209 Section Lotus, L.corniculatus group. L.corniculatus, L.glaber, L.uliginosus. Seed. Taxonomy. Natural hybridization. Commercial production and sale of Lotus. entry last revised Oct 13 2003 entry last revised Oct 28 2003
Lene Krusell R. H. Leep Max Planck Institute of Molecular Plant Michigan State University Exp Station Physiology 103 University Drive MPI-MP Chatham MI 49816 Am Mühlenberg 1 U.S.A. 14476 Golm [email protected] Germany Phone: 906-228-4830 [email protected] Fax No.: 906-228-4572 Phone: +49-331-5678150 L. corniculatus. Forage; seed. Seed production; L.japonicus. weed control in forage and pasture. entry last revised Oct 16 2003
Viviana Lepek Manuel Lainz CONICET researcher and Assistant Professor Real Instituto de Estudios Asturianos of Universidad de Gral San Martín Apartado 425 Instituto de Investigaciones Biotecnológicas E-33280 Gijon (Asturias) IIB-UNSAM Spain Departamento de Microbiología (Interacción Phone: 34-85-394911 Rhizobium-leguminosas) Fax No.: 34-85-381511 Av. Gral Paz entre Albarellos y Constituyentes Lotus spp. of the Iberian Pennisula and nearby INTI, Edif. 24 (Colectora Gral Paz 5445) areas. Taxonomy. (1650) San Martín Buenos Aires Argentina Pedro Laterra [email protected] Universidad Nacional de Mar del Plata http://www.iib.unsam.edu.ar Facultad de Ciencias Agrarias L.glaber, L.japonicus. Microbiology. CC 276, 7620 Balcarce entry last revised Nov 7 2003 Prov. Buenos Aires Argentina [email protected] Lina A.C. Lett http://www.mdp.edu.ar Assitant Professor Phone: +54-2266-439100 Facultad de Agronomía Fax No.: +54-2266-439101 Universidad Nacional del Centro de la L. glaber. Ecology. Provincia de Buenos Aires entry last revised Oct 29 2003 Ciencias Básicas Agronómicas y Biológicas Av. República Italia 780 C.C. 47, (7300) Azul W.H. (Bill) Leakey Provincia de Buenos Aires. Deer Creek Seed Argentina Box 105 [email protected] Ashland, WI 54806 http://www.faa.unicen.edu.ar U.S.A. Phone : +54-2281-433291/2/3 [email protected]
50 Lotus researchers.
Fax No.: +54-2281-433292 L.glaber, L.corniculatus. Microbiology, Lene Heegaard Madsen Molecular Biology, Forage production, IMSB-Lab. of Gene Expression Genetics. University of Aarhus entry last revised Nov 10 2003 Science Park Gustav Wieds Vej 10 C DK-8000 Aarhus C Milla Linde Denmark Swedish University of Agricultural Sciences Fax.no.: +45 86 12 31 78 Department of Crop Production Sciences Box 7043 S75007 Upsalla Sweden Antonio J. Marquez Profesor Titular Departamento de Bioquímica Vegetal y Biología Molecular Dasharath Prasad Lohar Facultad de Química Research Associate Apartado 553 University of Minnesota, St. Paul 41080-Sevilla Department of Plant Biology Spain 1445 Gortner Ave. [email protected] 250 Biological Sciences Building Phone: +34-95-4557145 Saint Paul, MN 55108 Fax.no.: +34-95-4626853 U.S.A. L. japonicus. Biochemistry, Molecular biology, [email protected] Biotechnology, Nitrogen assimilation, Phone: +1-612-6249230 Enzymology, Mutagenesis Fax No.: +1-612-6251738 entry last revised Oct 7 2003 L.japonicus. Molecular biology, microbiology, tissue culture, biology, genetics. entry last revised Oct 17 2003 J. H. McAdam Principle Scientific Officer Agriculture and Food Science Centre W. L. (Bill) Lowther Applied Plant Science Research Division Scientist Newforge Lane AgResearch Belfast BT9 5PX Plant Breeding and Genomics Northern Ireland Invermay Agricultural Centre [email protected] Private Bag Phone: +44-28-90255275 Mosgiel Fax No.: +44-28-90255003 New Zealand L.uliginosus. Ecology. Utilisation. [email protected] entry last revised Nov 21 2003 http://www.agresearch.co.nz Phone: +64-3-4899053 Fax No.: +64-3-4893739 Mark McCaslin L.uliginosus, L.corniculatus. Ecology; Forage Genetics utililization. microbiology, inoculation, N5292 Gills Coulee Rd. rhizobia. West Salem WI 54669 entry last revised Oct 23 2003 U.S.A. [email protected]
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Phone: +1-608-7862121 L.corniculatus, L.glaber, L.uliginosus, Fax No.: +1-608-7862193 L.japonicus. Biology, Physiology, Tissue L. corniculatus. Forage; seed. Culture, Molecular Biology, Microbiology. entry last revised Nov 5 2003 entry last revised Nov 18 2003
Robert L. McGraw Ken Moore Associate Professor of Agronomy Professor University of Missouri Iowa State University Department of Agronomy Department of Agronomy 208 Waters 1567 Agronomy Hall Columbia, MO 65211 Ames Iowa 50011 U.S.A. U.S.A. [email protected] [email protected] Phone: +1-314-882-6608 http://www.public.iastate.edu/~kjmoore/ Fax No.: +1-314-882-1467 Phone: +1-515-2943160 L. corniculatus. Forage; utilization; Fax No.: +1-515-2943163 physiology. Management strategies to improve L.corniculatus. Forage, utilization, ecology, performance and persistence. physiology. entry last revised Nov 30 2003 entry last revised Oct 27 2003
Réal Michaud Phil Morris Agriculture et Agroalimentaire Canada / Institute for Grassland & Environ. Res Agriculture and Agri-Food Canada Plas Gogerddan Aberystwyth Centre de recherche et de développement sur Dyfed SY23 3EB les sols et les grandes Cultures / Soils and Wales Crops Research and Development Centre [email protected] 2560 Hochelaga Blvd. Phone: 44 (0) 1970-823-113 Ste-Foy Québec G1V 2J3 Fax No.: 44 (0) 1970-823-242 Canada L.corniculatus, L.japonicus. Physiology; tissue [email protected] culture; biotechnology. Genetic manipulation Phone: +1-418-6577980 ext. 261 of secondary metabolism: effect of Fax No.: +1-418-6482402 environmental stress on secondary metabolism L.corniculatus. Forage; utilization. and digestibility; induced defense responses. entry last revised Oct 29 2003
Jorge A. Mosjidis Professor Jorge Monza Auburn University Professor Department of Agronomy and Soils Facultad de Agronomía 202 Funchess Hall Departmento de Biología Vegetal, Bioquímica Auburn, AL 36849-5412 Av. Garzón 780 U.S.A. Montevideo [email protected] Uruguay http://www.ag.auburn.edu/ay/mosjidis [email protected] Phone: +1-334-8443976 http://www.fagro.edu.uy/bioquim/web Fax No.: +1-334-8443945 Phone: ++598-2-3540229 Genetics. Fax No.: ++598-2-3543004 entry last revised Nov 6 2003
52 Lotus researchers.
[email protected] John Mundy Phone: +81-172-393776 Professor Fax No.: +81-172-393750 Institute of Molecular Biology L.corniculatus and L.japonicus. Genetics, Oester Farimagsgade 2A Breeding and Molecular Biology. Plant 1353 Copenhagen K breeding and Genetics by using somatic cell Denmark hybridization and Somaclonal variation. [email protected] entry last revised Oct 18 2003 http://www.biobase.dk/~mundy Phone: +45-35322131 Fax No.: +45-35322128 Takuji Ohyama Lotus japonicus. Professor entry last revised Oct 16 2003 Faculty of Agriculture, Niigata University Department of Applied Biological Chemistry 2-8050 Ikarashi, Niigata, 950-2181 Valeria Negri Japan Associate professor [email protected] Universita' Degli Studi Di Perugia Phone: +81-25-2626643 Biologia Vegetale Biotecnologie Fax No.: +81-25-2626643 Agroambientali L.japonicus. Genetics, Physiology, Symbiosis, Borgo XX Giugno 74 Molecular Biology, Microbiology. 06100 Perugia entry last revised Nov11 2003 Italy [email protected] http://www.agr.unipg.it/dbvba Fernando Olmos Phone: +39 075 5856218 Assistant Researcher Fax No.: +39 075 5856224 Instituto Nacional Investigación Agropecuaria Lotus curator. Germplasm, genetics. - INIA entry last revised Nov 25 2003 Pasture Ecology Ruta 5, Km. 386 Tacuarembo C. J. Nelson Uruguay University of Missouri [email protected] 210 Waters Hall Phone: +598-63-22407 Columbia, MO 65211 Fax No.: +598-63-23969 U.S.A. L.corniculatus; L.subbiflorus; L.uliginosus. [email protected] Germplasm, Ecology, Physiology, Forage Phone: 314-882-2801 Production. Fax No.: 341-882-1467 entry last revised Nov 5 2003 L. corniculatus. Physiology; forage.
Alicia Orea Minoru Niizeki Postdoctoral position Professor Instituto de Bioquímica Vegetal y Fotosíntesis Hirosaki Unibersity CSIC-Universidad de Sevilla Faculty of Agriculture and Life Science Avda. Américo Vespucio, s/n Plant Breeding and Genetics 41092-Seville Hirosaki Spain Aomori-ken 036-8561 [email protected] Japan http://www.ibvf.cartuja.csic.es/ Phone: +34-95-4489526
Lotus researchers. 53
Fax No. : +34-95-4460065 Lotus japonicus. Physiology. Nitrogen Peter Paľove-Balang assimilation. Researcher entry last revised Oct 31 2003 Institute of Botany SAV Department of Plant Physiology Dúbravská cesta 14 María Cristina de Pablo Bratislava Profesor Adjunto, con dedicación Exclusiva Slovak Republic, 845 23 Universidad Nacional del Centro de la [email protected] Provincia de Buenos Aires (UNCPBA) Phone: +421-2-59426127 Facultad de Agronomía Fax No.: +421-2-54771948 Laboratorio Regional de Análisis de Semillas y L.japonicus. Physiology, Molecular biology. Granos entry last revised Oct 11 2003 Av. República Italia 780, C.C. 47 (7300) Azul Provincia de Buenos Aires Argentina Yousef A. Papadopoulos [email protected] Agriculture and Agri-Food Canada http://www.faa.unicen.edu.ar 14 Fundy Drive Phone: +54-2281-427566 Truro, NS B2N 5Z3 Fax No.: +54-2281-433292 Canada L.glaber, L.corniculatus. Seed Production and [email protected] Quality. Phone: +1-902-8960400 entry last revised Nov 4 2003 Fax No.: +1-902-8960200 L.corniculatus. Genetics; breeding; forage. Developing germplasm for seedling vigor and competitive ability for cultivar development. Cristina Pacios-Bras entry last revised Oct 22 2003 Postdoctoral position Leiden University Laboratoire des Proteines du Cytosquelette 41, Rue Jules Horowitz Martin Parniske F-38027 Grenoble Cedex 1 Research Scientist France John Innes Centre [email protected] The Sainsbury Laboratory Phone: +33-4-38789219 Colney Lane Fax No.: +33-4-38785494 Norwich NR4 7UH Former Lotus japonicus research. England entry last revised Oct 31 2003 [email protected] http://www.jic.bbsrc.ac.uk/science/sl/smp.h tm Phone: +49-1603-450249 Eloisa Pajuelo L.japonicus. L.filicaulis. Genetic and physical Department of Biological Molecular mapping of plant genes. Molecular genetic University of Sevilla analysis of pathogenic and symbiotic plant- Apdo. 553 microbe interactions. Nitrogen fixing root 41080 Sevilla nodule and arbuscular mycorrhiza symbiosis. Spain entry last revised Oct 25 2003 [email protected]
54 Lotus researchers.
Andrea Pedrosa-Harand Braj Nandan Prasad PostDoc Professor & Coordinator,Programme in Universidade Federal de Pernambuco Biotechnology Centro de Ciências Biológicas Tribhuvan University Departamento de Botânica Programme in Biotechnology C/O Central Laboratório de Citogenética Vegetal Department Of Botany Rua Nelson Chaves S/N, Cidade Universitária Kirtipru 50.670-420 Recife - PE P.O.Box 9782 Brazil Kathmandu [email protected] Nepal [email protected] [email protected] Phone: +55-81-32718846 Phone: +977-1-330582 Fax No.: +55-81-32718348 Fax No: +977-1-4331964 L.japonicus, L.filicaulis, L.burttii. Genetics L.corniculatus and L.japonicus. Physiology (Cytogenetics, Physical mapping, Comparative and Molecular Biology. Genomics), Taxonomy. entry last revised Oct 18 2003 entry last revised Nov. 23 2003
Mette la Cour Petersen Daniel Real The Royal Veterinary and Agricultural INIA - Tacuarembo University Ruta 5 Km. 386 Department of Plant Biology C.P. 45000 Tacuarembo Plant Physiology and Anatomy Lab. Uruguay Thorvaldsensvej 40 [email protected] DK-1871 Frederiksberg C Phone: 598-632-2407 Denmark Fax No.: 598-632-3969 [email protected] More than 40 Lotus species. Genetics; Fax.no.: +45 35 28 33 10 breeding. Plant introduction trials for the genus Lotus in the basaltic region of Uruguay.
Carsten Poulsen Mónica Rebuffo University of Aarhus National Institute of Agricultural Research Dep.Mol.Biol. - Lab.Gene Expression INIA “La Estanzuela” Science Park Ruta 50, km 11 Gustav Wieds Vej 10 C C.P. 70000 Colonia DK-8000 Aarhus C Uruguay Denmark [email protected] [email protected] http://www.inia.org.uy http://130.225.13.27/~chp/ http://www.inia.org.uy/sitios/lnl/ Phone: +45-89425007 Phone: +598-574-8000 Ext 1479 Fax.no.: +45-86123178 Fax No.: +598-574-8012 L.japonicus. Genetics, Molecular Biology, L.corniculatus, L.uliginosus, L.subbiflorus. Microbiology. Breeding. Genetics. Lotus Curator. Lotus entry last revised Oct 16 2003 Newsletter editor. entry last revised Oct 2 2003
Lotus researchers. 55
http://www.psu.missouri.edu/roberts/ Kevin Reed Phone: +1-573-8822801 Department of Primary Industries Fax No.: +1-573-8844317 Pastoral and Veterinary Institute Mainly L.corniculatus, but other species as PB 105 well. Germplasm screening. Forage quality. Hamilton, Victoria 3300 Biochemical defense. Australia entry last revised Oct 22 2003 [email protected] Phone: +61-55-730911 Fax No.: +61-55-711523 Mark P. Robbins L uliginosus; L.corniculatus.Ecology; forage; Institute for Grassland & Environ. Res. utilization; germplasm. Plant introduction, Cell Biology Dep. cultivar evaluation, and animal production. Plas Gogerddan Aberystwyth entry last revised Oct 12 2003 Dyfed WALES SY23 3EB UK [email protected] J.S. Grant Reid Phone: 44 (0) 1970-823-113 Professor Emeritus Fax No.: 44 (0) 1970-823-242 University of Stirling L. corniculatus; L. japonicus. Genetics; Department of Plant Biochemistry biology; biotechnology; secondary products. Stirling FK9 4LA Analysis and genetic modification of Scotland flavonoids and condensed tannins in L. United Kingdom corniculatus. Identification and [email protected] characterization of tannin genes in L. Phone: +44 1786 467762 japonicus. Fax No.: +44 1786 464994 L. japonicus.Physiology, Molecular Biology. entry last revised Nov 30 2003 William John Rogers Professor Tit. Ord. Excl. Facultad de Agronomía, Universidad Nacional Joel Reynaud del Centro de la Provincia de Buenos Aires Laboratoire de Botanique Ciencias Básicas Agronómicas y Biológicas Faculté de Pharmacie Av. República Italia 780, C.C. 47 Avenue Rockefeller (7300) Azul 69373 Lyon Cedex 08 Provincia de Buenos Aires. France Argentina [email protected] [email protected] http://ispb.univ-lyon1.fr/cours/botanique http://www.faa.unicen.edu.ar Teaching (currently no research activity with Phone: +54-2281-433292 Lotus). Fax No.: +54-2281-433292 entry last revised Nov 30 2003 L.corniculatus, L.glaber. Genetics, Breeding,
Molecular Biology. entry last revised Nov 25 2003 Craig A. Roberts Associate Professor University of Missouri Clive Ronson Agronomy Department Chair in Genetics 214 Waters Hall University of Otago Columbia, MO 65211 Microbiology Department U.S.A. P.O. Box 56 [email protected]
56 Lotus researchers.
Dunedin P.O. Box 49 New Zealand DK-4000 Roskildie [email protected] Denmark http://microbes.otago.ac.nz/dept/STAFF/pr [email protected] ofile-ronsonc.html Fax.no.: +45 46 77 42 82 Fax No.: +64-4798540 L.japonicus, L.corniculatus. Microbiology and Genetics of the microsymbiont Mesorhizobium Kazuhiko Saeki loti. Department of Biology entry last revised Oct 9 2003 Graduate School of Science Osaka University 1-1 Machikaneyama Andreas Roussis Toyanaka 560-0043 IMSB-Lab. of Gene Expression Japan University of Aarhus [email protected] Science Park Fax.no.: +81 6 850 5425 Gustav Wieds Vej 10 C DK-8000 Aarhus C Denmark Hajime Sakai Fax.no.: +45 86 12 31 78 DuPont Agricultural Biotech 1 Innovation Way Newark, DE 19714-6104 Oscar Adolfo Ruiz U.S.A. Researcher (CONICET) and Professor [email protected] (UNSAM) Fax.no.: +302 631 2607 Instituto de Investigaciones Biotecnológicas- Instituto Tecnológico de Chascomús (IIB- INTECh) and Universidad Nacional de San Federico Sanchez Martín (UNSAM) Professor Unidad de Biotecnología 1 IIB-INTECh and Universidad Nacional Autónoma de México. Licenciatura en Biotecnología (UNSAM) Plant Molecular Biology Department. Instituto Casilla de Correo 164. de Biotecnología Camino de circunvalación Km 5. Av. Universidad 2001 (B7130 IWA). Chascomús Col. Chamilpa. 62210 Provincia de Buenos Aires Cuernavaca, Morelos Argentina México [email protected] [email protected] [email protected] Fax No.: +52-73-172388 http://www.iib.unsam.edu.ar L. japonicus, L. corniculatus.Molecular and Phone: +54-2241-424049/430323 cell biology. Cell imaging. Actin Fax No.: +54-2241-424048 cystoskeleton, cell signaling. Nodule L.corniculatus, L.glaber, and L.japonicus. development. Rhizobium-Legume interactions. Biology, Physiology, Molecular Biology and entry last revised Nov 30 2003 Microbiology. entry last revised Oct 6 2003 Niels Sandal IMSB-Lab. of Gene Expression Gerhard Saalbach University of Aarhus Risø National Laboratory Gustav Wieds Vej 10 C Plant Biology & Biogeochemistry Department DK-8000 Aarhus C
Lotus researchers. 57
Denmark Science Park [email protected] Gustav Wieds Vej 10 C Fax.no.: +45 86 12 31 78 DK-8000 Aarhus C Denmark [email protected] Juan Sanjuán Fax.no.: +45 86 12 31 78 Research Scientist Consejo Superior de Investigaciones Científicas (CSIC) Simone Meredith Scheffer-Basso Estación Experimental del Zaidin Professor Departamento de Microbiologia del Suelo y Instituto de Ciências Biológicas/ Universidade Sistemas Simbióticos de Passo Fundo Prof. Albareda 1 Departamento Biologia E-18008 Granada Rua Silva Jardim, 303 apto. 701 Spain 99010-240 Passo Fundo [email protected] Rio Grande do Sul http://www.eez.csic.es/inves/grupos/D3.htm Brazil Phone: +34-958-181600 Ext. 259 [email protected] Fax No.: +34-958-129600 http://www.upf.tche.br L.japonicus, L.corniculatus, L.glaber, Phone: +55-316-8100 Ext: 8326 L.uliginosus. Microbiology. L.corniculatus. Morphophysiology and entry last revised Oct 13 2003 evaluation of forage production (cutting- grazing tolerance, growth habit , nutritive value, competition, mixtures with grasses). Sindhu Sareen entry last revised Oct 16 2003 Indian Grassland and Fodder Research Institute CSKHPKV Campus Palampur 176062 (H.P.) Helmi R.M. Schlaman India Postdoctoral [email protected] Leiden University entry last revised Oct 9 2003 Institute of Biology Leiden Wassenaarseweg 64 2333 AL Leiden Shusei Sato The NetherIands Researcher [email protected] Kazusa DNA Research Institute Phone: +31-71-5274927 Plant gene research group Fax No.: +31-71-5275088 2-6-7 Kazusa-Kamatari L.japonicus. Molecular biology Kisarazu, 292-0818 entry last revised Oct 20 2003 Japan [email protected] http://www.kazusa.or.jp/lotus/ Ms Anne Schneider Phone: +81-438-523935 Executive Secretary Delegate Fax No.: +81-438-523934 AEP, European Association for Grain Legume L.japonicus. Genomics; Molecular genetics. Research entry last revised Nov 24 2003 Executive Secretariat 12 avenue George V 75 008 Paris Leif Schauser France IMSB-Lab. of Gene Expression [email protected] University of Aarhus
58 Lotus researchers. http://www.grainlegumes.com Russia Phone: +33-1-40694909 [email protected] Fax No.: +33-1-47235872 http://www.herba.msu.ru/ The AEP is an international multidisciplinary Phone: +7-095-9391603 network of scientists and end-users concerned Fax No.: +7-095-9392777 with grain legumes. The AEP office is L.australis and related taxa, L.arabicus and interested in being informed about the progress related taxa, L.discolor and related taxa, in Lotus investigations for establishing L.creticus and related taxa. Taxonomy. connection with other legumes research Generic limits of Lotus, taxonomy of its activities. segregate genera, sectional system of Lotus, entry last revised Nov 20 2003 taxonomy of some white-, red- and pink- flowered species. Cladistic analyses. Flower development in Lotus corniculatus. Philippe Seguin entry last revised Oct 9 2003 Assistant Professor McGill University, Macdonald Campus Department of Plant Science Diego Sorrondegui Larrosa 21,111 Lakeshore Rd. Gabriel A. Pereyra 2940/205 Ste. Anne de Bellevue 11300 Montevideo Quebec H9X 3V9 Uruguay Canada [email protected] [email protected] FAX: 598-2-707-87-45 http://www.mcgill.ca/plant/faculty/seguin/ L. corniculatus. Breeding; ecology; Phone: +1-514-3987851 germplasm;seed; forage. Persistence of Lotus. Fax No.: +1-514-3987897 L.corniculatus. Forage production, Utilization. entry last revised Nov 21 2003 Gary Stacey University of Tennessee Center for Legume Research Richard R. Smith M409 Walters Life Science Bldg. Retired Research Geneticist University of Tennessee, Knoxville, TN USDA-ARS, University of Wisconsin 37996-0845 US Dairy Forage Research Center U.S.A. 1925 Linden R. West [email protected] Madison WI 53706 http://www.bio.utk.edu/microbio/staceylab/ U.S.A. nodulate.html [email protected] Telephone 423-974-4041 Phone: +1-608-2645240 Fax.no.: 423-974-4007 Fax No.: +1-608-2645147 L. corniculatus. Genetics; breeding; forage. Selection for improved seedling establishment Jiri Stiller and improved persistence; cultivar evaluation. Plant Molecular Genetics Center for Legume entry last revised Nov 11 2003 Research
The University of Tennessee
Knoxville, TN 37901-1071 Dmitry Sokoloff U.S.A. Lecturer [email protected] Higher Plants Department http://soybean.ag.utk.edu Biological Faculty
Moscow State University
119992 Moscow
Lotus researchers. 59
Canada Jens Stougaard [email protected] Lecturer, Group leader Phone: +1-519-457-1470 ext. 273 University of Aarhus Fax.no.: +1-519-457-3997 IMSB-Lab. of Gene Expression L.japonicus Department of Molecular Biology entry last revised Oct 02 2003 Science Park Gustav Wieds Vej 10 C DK-8000 Aarhus C Shigeyuki Tajima Denmark Professor [email protected] Lab. Molecular Plant Nutrition Phone: +45-89425011 Faculty of Agriculture Fax.no.: +45-8621222 Kagawa University L.japonicus. Plant molecular genetics and Miki-cho, Kita-gun general molecular biology. Genetic mapping, Kagawa 761-0795 map-based cloning, gene isolation, gene Japan characterisation, expression studies. [email protected] entry last revised Oct 15 2003 Phone: +81-87-8913129 Fax.no.: +81-87-8913021 L.japonicus. Genetics, Molecular Biology. Norio Suganuma entry last revised Oct 17 2003 Professor Aichi University of Education Department of Life Science Myra Tansengo Kariya Department of Biotechnology Aichi 448-8542 Faculty of Engineering Japan Osaka University [email protected] Rm. 202, Akatsuka-so 1-9-2, Kamishinden Phone: +81-566-262647 Toyohaku-shi, Osaka 560-0085 Fax No.: +81-566-262310 Japan L. japonicus. Genetics, Physiology. [email protected] entry last revised Nov 5 2003 L. japonicus. Biotechnology. In planta transformation; mutation studies.
John Sullivan Research fellow Nancy Terryn University of Otago Researcher Department of Microbiology University Gent New Zealand Instititute plant Biotechnology for developing [email protected] Countries Phone: +64-3-4798373 K.L. Ledeganckstraat 35 Fax No.: +64-3-4798540 9000 Gent L.corniculatus, L.japonicus. Molecular Belgium Biology, Microbiology. [email protected] entry last revised Nov 20 2003 http://www.ipbo.ugent.be/ Phone: +32-9-2645201 Fax No.: +32-9-2648795 Krzysztof Szczyglowski L.japonicus, Phaseolus, Lathyrus. Agriculture & Agri-Food Canada Transformation, seed development, stress 1391 Sandford Street response, molecular biology of Grain legumes London, ON N5V 4T3
60 Lotus researchers. such as Phaseolus, cowpea, Lathyrus for which Symbiotic nitrogen fixation and molecular there is little genomic data so we look at model plant nutrition. systems L.japonicus. entry last revised Oct 22 2003 entry last revised Oct 20 2003
Yosuke Umehara Monica Tourn Laboratory of Nitrogen Fixation Catedra de Botanica Department of Plant Physiology Fac. Agronomia-UBA National Institute of Agrobiological Resources Avda. San Martin 4453 1-2 Kannodai 2-chome RA-1417 Buenos Aires Tsukuba, Ibaraki 305-0856 Argentina Japan [email protected] [email protected] Phone: 522-0903 Fax.no.: +81-298-38-8347 Fax No.: 522-1687 L.glaber. Ecology; forage. Architectural analysis and clonal growth. K. Urbanska Geobotanisches Institut E.T.H. Zurich Toshiki Uchiumi 38 Zurichbergstrasse Associate Professor CH-8044 Zurich Kagoshima University Switzerland Faculty of Science Phone: 0041-1-252- Department of Chemistry and BioScience Fax No.: 0041-1-252-3404 1-21-35 Korimoto L. corniculatus. Ecology; reclamation. Kagoshima 890-0065 Japan [email protected] Carroll Vance Phone: +81-99-2858164 USDA-ARS, Agronomy & Plant Genetics Fax.no.: +81-99-2858163 University of Minnesota L.japonicus. Physiology, Molecular Biology, 1991 Buford Circle Microbiology, Symbiosis St. Paul MN 55108 entry last revised Oct 21 2003 U.S.A. [email protected] Phone: 612-625-1991 Michael Udvardi Fax No.: 612-625-1268 Group Leader L.corniculatus. Physiology. Carbohydrate and Max-Planck-Institut fur Molekulare nitrogen metabolism research on Pflanzenphysiologie L.corniculatus. Molecular Plant Nutrition Am Mühlenberg 1 14476 Golm Brad Venuto Deutchland 215 M. B. Sturgis Hall [email protected] Louisiana State University Phone: +49-331-5678149 Agronomy Department Fax No.: +49-331-5678250 Baton Rouge, LA 70803 L.japonicus. Genetics, biochemistry, molecular U.S.A. biology, and functional genomics [email protected] (transcriptomics, proteomics, and metabolics).
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Desh Pal S. Verma Ken P. Vogel Professor USDA-ARS Dept. of Agronomy Ohio State University 332 Keim Hall, East Campus 1060 Carmack Rd. Univ. of Nebraska Columbus OH 43210-1002 Lincoln, NE 68583-0937 U.S.A. U.S.A. [email protected] [email protected] L. corniculatus. Genetics; tissue culture; Phone: 402-472-1490 biotechnology. Expression of nodule-specific Fax No.: 402-437-5254 genes. L. corniculatus. Genetics; breeding. Interested in compatibility of L. corniculatus with native grass species. Don R. Viands Associate Dean and Director of Academic Programs, Professor of Plant Breeding Dept. of Jeffrey J. Volenec Plant Breeding and Biometry Professor 151 Roberts Hall Purdue University Ithaca, NY 14853 Department of Agronomy U.S.A. Lilly Hall of Life Sciences [email protected] 915 West State St. Phone : +1-607-255-3081 West Lafayette IN 47907-2054 Fax No.: +1607-254-4613 U.S.A. L.corniculatus. Breeding; pathology. Breeding [email protected] for resistance to crown-rot and to Fusarium http://www.agry.purdue.edu/staffbio/jjvbio. wilt caused by Fusarium oxysporum. htm entry last revised Oct 7 2003 Phone: +1-765-494-8071 Fax No.: +1-765-496-2926 L.corniculatus. Physiology; utilization, molecular biology, biochemistry. Osvaldo Ramón Vignolio entry last revised Oct 27 2003 Professor Facultad de Ciencias Agrarias. Universidad Nacional de Mar del Plata- EEA INTA Trevor L. Wang Balcarce. Group Leader Producción Vegetal Metabolic Biology Department Ruta 226, Km 73.5 John Innes Centre CC 276 (7620) Balcarce Norwich Research Park Argentina Norwich NR4 7UH [email protected] UK [email protected] [email protected] [email protected] http://www.jic.bbsrc.ac.uk/staff/trevor- Phone: +54-2266-439100 wang/index.htm Fax No.: +54-2266-439101 Phone: +44-1603-450283 L.corniculatus, L.glaber. Germplasm, Ecology, Fax No.: +44-1603-450014 Biology, Physiology, Forage Production, L.japonicus. Genetics, Biology, Physiology, Utilization, Seed Production. Molecular Biology, Metabolism entry last revised Nov 9 2003 entry last revised Nov 4 2003
62 Lotus researchers.
Judith Webb Research Scientist Institute of Grassland and Environmental Research Plas Gogerddan Aberystwyth Ceredigion, SY23 3EB Wales, UK [email protected] Phone: +44-1970-823124 Fax No.: +44-1970-823243 L.japonicus, L.corniculatus. Genetics, Biology, Physiology, Tissue Culture, Molecular Biology, Microbiology entry last revised Nov 9 2003
Susan Wijting Leiden University Clusius Laboratorium Wassenaarse Weg 64 2333 AL Leiden The Netherlands [email protected]
Lotus Newsletter (2003) Volume 33, 63 – 96.
Lotus literature Database provided by Lotus Newsletter recipients, last updated Nov. 30 2003
ALI S.I. and SOKOLOFF D.D. 2001. A new combination in Pseudolotus Rech. f. (Leguminosae: Loteae). Kew Bull., 56, 721-723. Pseudolotus is a genus segregated from Lotus.
ALLAN G.J., ZIMMER E.A., WAGNER W.L. and SOKOLOFF D.D. 2003. Molecular phylogenetic analyses of tribe Loteae (Leguminosae): implications for classification and biogeography. In: Klitgaard B.B. and Bruneau A. (eds.). Advances in Legume Systematics. Pt. 10, Higher Level Systematics. Kew: Royal Botanic Gardens, pp. 371-393. [In press]
ALTIER N. 2003. Caracterización de la población de Fusarium oxysporum y potencial patogénico del suelo bajo rotaciones agrícola ganaderas. In: Rotaciones. Montevideo, INIA. Serie Técnica, No.134, 37-44. [Altier N. 2003. Characterization of the population of Fusarium oxysporum and soil pathogenical potential under crop-pasture rotations. In: Rotations. Montevideo, INIA. . Serie Técnica, No.134, 37-44. [In Spanish]]. During 1996-2002, soil samplings were performed three times per year in a crop rotation experiment established in 1963 at INIA la Estanzuela, Uruguay. Soil population of Fusarium oxysporum and pathogen potential were estimated for four cropping systems differing in the pasture type (legume or grass-legume) and pasture duration (0-4 years). Recovery of F. oxysporum, the primary pathogen associated with crown and root rot of forage legumes, was proportional to the time that the soil remained under legume pasture. Average CFU/g of soil were 1678, 2967, 2990 and 3934, for 100% agriculture – no pasture, 50% agriculture – 50% birdsdfoot trefoil pasture, 50% agriculture – 50% grass-legume pasture and 33% agriculture – 66% red clover and grass-legume pasture, respectively. Isolates of F. oxysporum recovered from different systems were pathogenic to seed and seedlings of birdsfoot trefoil and red clover; however, significant differences in aggressiveness were observed among them. Isolate aggressiveness to birdsfoot trefoil was higher than to red clover, as expressed by percentage of surviving plants (28.7% and 58.6%, respectively). When sown in soil under legume pasture, birdsfoot trefoil productivity was significantly more depressed than red clover productivity, as expressed by emergence and total biomass. The highest pathogen potential to both legume species was recorded under the system with 50% birdsfoot trefoil pasture. We concluded that the crop sequence that characterizes each system has a long-term effect on the soil fungal populations. Thus, crop rotation needs to be considered as a means for disease management, which contributes toward legume persistence and sustainable cropping systems.
ALTIER N., PÉREZ C., DE LA FUENTE L. and ARIAS A. 2000. Native fluorescent Pseudomonas as biocontrol agents of seedling diseases on birdsfoot trefoil. Proceedings of the Fifth International PGPR Workshop. October 29 – November 3, 2000. Córdoba, Argentina. http://www.ag.auburn.edu/argentina/pdfmanuscripts/altier.pdf
ARANGO N., JACOBS B.C. and BLUMENTHAL M.J. 1998. Seed production of Lotus uliginosus
63 64 Lotus literature
cv. Sharnae in response to plant population density. Australian Journal of Experimental Agriculture, 38, 837-842.
ARCHER A.C., WILSON G.P.M. and WALKER R. 1981. Lotus species. NSW Agriculture Agfact P2.5.12, first edition.
ARMSTEAD I.P. and WEBB K.J. 1987. Effect of age and type of tissue on genetic transformation of Lotus corniculatus by Agrobacterium tumefaciens. Plant Cell, Tissue and Organ Culture, 9, 95-101.
ARTOLA A., CARRILLO CASTAÑEDA G. and GARCÍA DE LOS SANTOS G. 2003. Hydropriming: a strategy to increase Lotus corniculatus L. seed vigor. Seed Science and Technology, 31, 455-463.
ARTOLA A., GARCÍA DE LOS SANTOS G. and CARRILLO CASTAÑEDA G. 2003. A seed vigor test for birdsfoot trefoil. Seed Science and Technology, 31, 753 -757.
AYRES J.F. 1997. Highlights of lotus grazing management research in NSW. Grassland Society of NSW Newsletter, 12, 20-21.
AYRES J.F. 1997. “Planned lotus planting can reap rewards”. Nornews Rural, September 1997, p 5.
AYRES J.F. 1999. Summary sheet – “Greater lotus”.
AYRES J.F. 2002. Co-learning in pasture R&D – the lotus project. Grassland Society of NSW Newsletter, 17, 18-21.
AYRES J.F. 2002. Co-learning in pasture R&D – the lotus project. District Agronomists Conference held at the Orange campus of the University of Sydney, 5-7 February 2002.
AYRES J.F. 2002. New lotus varieties for acid soils in northern NSW. Acid Soil Action Review Workshop, Agricultural Research Institute, Wagga Wagga, 2-4 September 2002.
AYRES J.F. and BLUMENTHAL M.J. 2000. Lotus grazing management for weaner production. Final Report on DAN 082 (1994/95 – 1998/99) for Meat & Livestock Australia
AYRES J.F. and BLUMENTHAL M.J. 2001. Lotus opens new opportunities for grazing. Farming Ahead 119 pp 58-59.
AYRES J.F., BLUMENTHAL M.J. and EARLY R. 2002. Lotus opens new opportunities for grazing. Grasslands Society of NSW Newsletter, 17, 12-14.
AYRES J.F., BLUMENTHAL M.J. and HOCHMAN Z. 1997. Grazing management of Lotus: use of co-learning to increase adoption of new cultivars. Wool and Sheepmeat Services Program Annual Conference, pp 42-44, October 21 - 23 1997, Orange. Lotus literature 65
AYRES J.F. and BOURKE C. 2003. Lotus – Birdsfoot trefoil. NSW Agriculture Agnote DPI- 413, first edition, January 2003.
AYRES J.F. and BOURKE C. 2003. Lotus – Greater lotus. NSW Agriculture Agnote DPI-412, first edition, January 2003.
AYRES J.F. and LLOYD DAVIES H. 1998. “Lotus use and agronomy”. Grasslands Society of NSW Newsletter, 3, 11-15.
AYRES J.F. and LLOYD DAVIES H. 1999. White clover improvement. Grassland Society of NSW Newsletter, 14, 10-12.
BASSO S.M.S., JACQUES A.V.A. e DALL'AGNOL M. 2002. Alocação da biomassa e correlações morfofisiológicas em leguminosas forrageiras com hábitos de crescimento contrastantes. Scientia Agricola, Piracicaba, 59, 629-634. [Basso S.M.S., Jacques A.V.A. and Dall'agnol M. 2002. Biomass allocation and morphophysiological correlations in forage legumes with contrating growth habits. Scientia Agricola, Piracicaba, 59, 629-634. [Portuguese]] The biomass allocation analysis and the correlation among morphophysiological variables allow greater understanding of the establishment, yield and persistence of perennial species in plant breeding projects, management and ecology of forage plants. To analyze the biomass allocation of forage legumes (Adesmia latifolia, A. punctata, A. tristis, Lotus corniculatus, L. uliginosus), an experiment was carried out under greenhouse conditions for 210 days (4000 degree-days); the plants were cultivated in 1 m2 wooden boxes. Plants were harvested to evaluate the morphological components and to describe their growth habit. The root, stem and leaf biomass allocations were expressed as dry mass percentages. The greatest allocation on leaves occurred for stoloniferous species, A. latifolia (63.5%) and A. punctata (61.4%), which presented the smallest allocation for roots, 10.9 and 14.7%, respectively. Largest stem biomass allocation (39.4%) and roots (24.4%) were found for L. uliginosus, a species with rhizomes. The leaf area index (LAI) was positively correlated with the number of leaves, secondary stems and aerial biomass. A. latifolia and Lotus spp. are extreme examples of the relative importance of morphological components in the formation of LAI and biomass. The accumulation pattern and forage allocation of stoloniferous legumes are mainly characterized by leaf production, pointing out the importance of stolon elongation and rooting, while for A. tristis and Lotus spp., the stem and root fractions had the same importance.
BASSO S.M.S, JACQUES A.V.A, DALL'AGNOL M., RIBOLDI J. e CASTRO S.M.J. 2000. Dinâmica da formação de gemas, folhas e hastes de espécies de Adesmia DC e Lotus L. Revista da Sociedade Brasileira de Zootecnia, Viçosa, 29, 1961-1968. [Basso S.M.S, Jacques A.V.A, Dall'Agnol M., Riboldi J. and Castro S.M.J. 2000. Dynamics of buds, leaves and stems formation of the Adesmia DC. and Lotus L. species. Revista da Sociedade Brasileira de Zootecnia, Viçosa, 29, 1961-1968. [Portuguese]] The genus Adesmia DC. has 17 indigenous species from southern Brazil. The species grow during winter-spring, and their forage potential has recently been studied. This work had as objective to observe the dynamics of bud (B), leaves (L) and stems (S) formation of A. latifolia, A. tristis and A. punctata, using Lotus corniculatus (birdsfoot-trefoil) and L. uliginosus as control species. The assay was conducted under greenhouse conditions, during 210 days (4000 degrees-days); during this period the plants were harvested six times, to evaluate the morphological components. There was a positive response of these variables to the accumulation of degrees day. The densities of B/m2 were, in average, 66 Lotus literature
4679 for Lotus spp., 3000 for A. tristis, 700 for A. punctata and 1200 for A. latifolia. The species of Lotus spp. showed a greater degree of branching (3600 S/m2) and leaf formation, estimated in 15500 L/M2 for birdsfoot-trefoil. In the stoloniferous, A. latifolia and A. punctata, the average percent of stems of the stolon type was approximately 90% and 50%, respectively, at the pre-inflorescence stage. A. tristis and birds foot-trefoil, with an erect growth habit, maintained a mixed population of erect and prostrate stems, while the rhizomatous L. uliginosus, showed 11% of rhizomes at the end of the experimental period. All legumes, but L. uliginosus, reached the flowering stage. A. latifolia maintained about 95 to 99% of its buds at the soil surface level (at the stolon nodes), a desirable characteristic for continuously grazed plants.
BASSO S.M.SCHEFFER., JACQUES A.V.A., DALL'AGNOL M., RIBOLDI J. and CASTRO S M J. 2001. Disponibilidade e valor nutritivo de forragem de leguminosas nativas (Adesmia DC) e exóticas (Lotus L.). Revista Brasileira de Zootecnia, Viçosa, MG, 30, 975-982. [BASSO S.M.SCHEFFER., JACQUES A.V.A., DALL'AGNOL M., RIBOLDI J. and CASTRO S M J. 2001. Availability and nutritive value of the wild (Adesmia DC.) and exotic legumes (Lotus L.). Revista Brasileira de Zootecnia, Viçosa, MG, 30, 975-982. [Portuguese]] The genus Adesmia DC. has 17 species native to Brazil, distributed in the Southern states, whose importance is linked to its adaptation to the soils and climatic conditions of the region, besides being an active winter-growing species (temperate). This work aimed to compare the patterns of dry matter (DM) accumulation and nutritive value of A. latifolia, A. punctata and A. tristis, using Lotus corniculatus (birdsfoot trefoil) and L. uliginosus (big trefoil), as checks. The experiment was carried out in the greenhouse for 210 days (4000 degrees-day). The forage availability (FA) was similar for A. latifolia (276 g DM/m2) and birdsfoot trefoil (275 g DM/m2), as well as for A. tristis (201 g DM/m2) and big trefoil (192 g DM/m2), while A. punctata showed the smallest FD (155 g DM/m2). A. latifolia was characterized by an early FA, due to its fast growth when compared to other species, pointing to its potential utilization during the cold season. In relation to the quality analysis, the crude protein (CP) in A. latifolia leaves was up to 21.6% and the organic matter in vitro digestibility (OMIVD) was up to 72.3%. The highest CP and OMIVD was found in the birdsfoot trefoil leaves, 30.3 and 75.8%, respectively. A. tristis presented a very low OMIVD in the stems, from 34.9 to 44.7%, which could limit its intake by cattle. It is concluded that, among the Adesmia species studied, A. latifolia holds the greatest forage potential and deserves further study.
BASTIANI PEREZ N. 2003. Melhoramento genético de leguminosas de clima temperado - alfafa (Medicago sativa L.) e cornichão (Lotus corniculatus L.) - para aptidão ao pastejo. Tese de Doutorado em Zootecnia, - Plantas Forrageiras, Faculdade de Agronomia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil. (159p.). Orientador: Miguel Dall’Agnol, Julho de 2003. [Bastiani Perez N. 2003. Breeding the temperate legumes – alfalfa (Medicago sativa L.) and birdsfoot trefoil (Lotus corniculatus L.) - to grazing aptitude. Doctoral thesis in Forage Science, Faculdade de Agronomia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil. (159p.) July, 2003. Adviser: Prof. Miguel Dall’Agnol. [Portuguese]] The objective of this work was to assess the genetic variability of different Crioula alfalfa and São Gabriel birdsfoot trefoil populations to grazing tolerance and to select plants more adapted to grazing. The plants were submitted to a heavy and continuous grazing (3-5 cm stubble). At the end of the experimental period there were significant differences (P< 0,05) among alfalfa populations but there were no consistent differences among birdsfoot trefoil treatment, which presented a higher percentage of surviving plants. The alfalfa’s morphological and physiological characteristics were evaluated in order to correlate then with grazing resistance. The results did not show significant differences (P> Lotus literature 67
0,05) among the genotypes in relation to crow contraction, crow area, leaf area, number of stems per plant, type of stem per plant (basilar or axillary), nitrogen content and non-structural carbohydrates of the roots. Greenhouse trials have identified morphological markers on alfalfa and birsfoot trefoil seedlings, which allowed an early identification of genotypes with grazing aptitude. In a greenhouse trial, the effectiveness of the recommend birdsfoot trefoil Rhizobium strains was evaluated in comparison with new strains collected, showing the possibility to isolate new strains. These results allowed the proposition of a new plant functional descriptor named specific buds for characterization of grazing aptitude. Besides that, the term grazing aptitude is proposed to replace grazing tolerance.
BAVAGE A.D., ROBBINS M.P., SKØT L. and WEBB K.J. 1998. Introducing and expressing genes in Legumes. In: Cunningham C. and Porter A. (Eds.) The production of recombinant proteins in plants. Methods in Biotechnology Vol.3 Humana Press. Totowa. N.J. (Dec.1997). pp. 27-38.
BLUMENTHAL M.J., AMPT P., HOCHMAN Z. and AYRES J.F. 1996. Grazing management of lotus: a participatory approach. Proceedings of the 8th Australian Agronomy Conferenc, p. 622.
BLUMENTHAL M.J., AYRES J.F. and HOCHMAN Z. 1997. Grazing management of Lotus in eastern Australia: A participatory approach. Proceedings of the Eighteenth International Grasslands Congress, 29.1-29.2.
BLUMENTHAL M.J., BOWMAN A.M., COLE A., JONES R.M., KELMAN W.M., LAUNDERS T.E. and NOCOL H.I. 1999. Establishment, growth and persistence of greater lotus (Lotus uliginosus) at six sites in eastern Australia. Australian Journal of Experimental Agriculture, 39, 819-827.
BLUMENTHAL M.J. and HARRIS C.A. 1998. Effects of Photoperiod and Temperature on Shoot, Root and Rhizome Growth in Three Lotus uliginosus Schkuhr Populations. Annals of Botany, 81, 55-59.
BLUMENTHAL M.J. and HARRIS C.A. 1993. Maku Lotus soil seedbanks in farmers’ fields. In ‘Proceedings 7th Australian Agronomy Conference’, Adelaide pp 414.
BLUMENTHAL M.J., HOCHMAN Z., AYRES J.F. and NICHOL H. 1995. Lotus Grazing Management for Weaner Production. MRC DAN 082 Protocol.
BLUMENTHAL M.J., KELMAN W.M., HOCHMAN Z. and AYRES J.F. 1995. Improving lotus persistence through breeding and management. Proceedings of the Tenth Annual Conference of the Grassland Society of NSW, p. 86.
BLUMENTHAL M.J., KELMAN W.M., HOCHMAN Z. and AYRES J.F. 1995. Improving lotus persistence through breeding and management. In: Michalk DL (ed) Latest Developments in Pasture Species, Annual Autumn Seminar, 1995, p 16.
BLUMENTHAL M.J., KELMAN W.M., LOLICATO S., HARE M.D. and BOWMAN A.M. 1993. 68 Lotus literature
Agronomy and improvement of Lotus: A review. Alternative Pastures Legumes, pp 74-85.
BLUMENTHAL M.J., KELMAN W.M., LOWTHER W.L., WIDDUP K.H. 1994. The use and management of Lotus in Australian and New Zealand. In ‘The 1st International Lotus Symposium’, 22-24 March 1994, Missouri, USA, pp 125-129.
BLUMENTHAL M.J. and MCGRAW R.L. 1999. Lotus Adaptation, Use, and Management. Trefoil: The science and Technology of Lotus. CSSA Special Publication, 28, Madison, USA.
BLUMENTHAL M.J., O'CONNOR J., AYRES J.F., LANE L.A., HOCHMAN Z. and HINDMARSH J. 1996. Improving lotus persistence through management: site establishment. Proceedings of the Eleventh Annual Conference of the Grassland Society of NSW, pp. 134-135.
BONFANTE P., GENRE A., FACCIO A., MARTINI, I., SCHAUSER, L., STOUGAARD J., WEBB K.J. and PARNISKE M. 2000. The Lotus japonicus LjSym4 gene is required for the successful symbiotic infection of root epidermal cells. Molecular Plant-Microbe Interactions, 13 (10), 1109-1120.
BONFANTE P., GENRE A., FACCIO A., NOVERO M., MARTINI I, SCHAUSER L, STOUGAARD J., WEBB K.J., and PARNIKE M 2000. The Lotus japonicus Sym4 gene is required for the successful infection of root cells by arbuscular mycorrhizal fungi. Second International Conference on the Model Legume Lotus japonicus. Norwich, U.K. 24-28th June 2000. Abs. No. S29.
BOWMAN A.M. 1993. Sharnae – a new Lotus pedunculatus for Australia. Lotus Newsletter, 24, 13-16.
CALLEJAS S., HIDALGO L.G., CAUHÉPÉ M.A. y OTERO M.J. 1999. Efecto del destete precoz sobre la performance reproductiva de vacas de cría multíparas. Revista Argentina de Producción Animal, 19, 323-329. [Callejas S., Hidalgo L.G., Cauhépé M.A. and Otero M.J. 1999. Effect of the early weaning on the reproductive performance of twin breeding cows. Revista Argentina de Producción Animal, 19, 323-329 [Spanish]].
CARRON T.R., MORRIS P., EVANS T.E., WEBB K.J. and ROBBINS M.P. 1992. Modification of tannin accumulation in Lotus corniculatus using heterologous antisense. SEB 5-10 April, University of Lancaster, UK. J. Expl. Bot. (suppl.) 43:250 (Abs. No. P10.83) p64.
CARRON T.R., MORRIS P., EVANS T.E., WEBB K.J. and ROBBINS M.P. 1991. Genetic modification of condensed tannin accumulation in transgenic Lotus corniculatus. 4th International Society of Plant Molecular Biology, Tucson, University of Arizona, USA, 6-12th October 1991. Abs. No. 1114.
CARRON T.R., MORRIS P., EVANS T.E., WEBB K.J. and ROBBINS M.P. 1991. Production and initial analysis of Lotus corniculatus hairy roots transformed with an antisense tannin biosynthetic gene. AFRC Plant Molecular Biology Meeting, University of Reading, UK.
Lotus literature 69
CARRON T.R., MORRIS P., EVANS T.E., WEBB K.J. and ROBBINS M.P. 1991. Modification of tannin biosynthesis in transgenic root cultures. SEB Meeting, University of Birmingham 7-12 April 1991. P8.69.
CHAMBERLAIN D.A. and WEBB K.J. 1986. A simple and convenient protoplast electrofusion methodology as used in attempts to optimise the production of forage legume heterokaryons. 6th International Congress of Plant Tissue and Cell Culture. University of Minnesota, USA. Abstract no 114.
CITERNE H.L., LUO D., PENNINGTON R.T., COEN E. and CRONK Q.C. 2003. A phylogenomic investigation of CYCLOIDEA-like TCP genes in the Leguminosae. Plant Phisiology, 131, 1042-1053.
COOK R., BARDGETT R.D., EASON W.R., SKØT L. and WEBB K.J. 2001. Biological interactions in grassland soils in relation to productivity. Brazil Abs No.
COOK R., BARDGETT R.D., EASON W.R., SKØT L. and WEBB K.J. 2001. Biological interactions in grassland soils and productivity. In: (Ed.) Grassland Ecosystems: an outlook into the 21st century. Proceedings of the XIX International Grassland Congress, Sao Paulo, Brazil, February 2001, pp.167-168.
COOKE D.E. and WEBB K.J. 1992. Stability of foreign gene expression (CaMV 35S-GUS) in hairy root cultures of Lotus corniculatus under different environmental regimes. SEB University of Lancaster, UK. 5th-10th April 1992. J. Expl. Bot. (suppl.) 43:250 (Abs. No. P10.16) p52.
COOKE D.E. and WEBB K.J. 1994 Considerations when using GUS activity in Lotus corniculatus. LOTUS Newsletter. Ed: PR Beuselinck USDA-Agricultural Research Service. 25:21-24.
COOKE D.E. and WEBB K.J. 1997. Stability of CaMV 35S-gus gene expression in (Bird's foot trefoil) hairy root cultures under different growth conditions. Plant, Cell, Tissue, Organ Culture, 47, 163-168.
COOMBER S, WEBB K.J. and PARNISKE M. 2000. Genes regulating arbuscular mycorrhiza in Lotus japonicus. Second International Conference on the Model Legume Lotus japonicus. Norwich, U.K. 24-28th June 2000. Abs. No. P15.
DALE P.J., FAY M.F., PIKE L.S., WOODCOCK S. and WEBB K.J. 1984. The transfer of new characters to forage legumes. British Grassland Society Occasional Symposium No 16. In: Forage Legumes. Ed: DJ Thomson 66-69.
DALE P.J., WEBB K.J., HALE A., DALTON S.J., PIKE L.S. and WOODCOCK S. 1984. Introducing new characters into forage grasses and legumes. Proceedings of 1st Welsh Research and Development Conference, University College of Wales, Aberystwyth.
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DE LA FUENTE L., QUAGLIOTTO L., BAJSA N., FABIANIO E., ALTIER A. and ARIAS A. 2000. Effect of the introduction of biocontrol Pseudomonas strains on the symbiosis rhizobia - forage legumes. October 29 – November 3, 2000. Proceedings of the Fifth International PGPR Workshop. Córdoba, Argentina. http://www.ag.auburn.edu/argentina/pdfmanuscripts/delafuente2.pdf
DE LA FUENTE L., QUAGLIOTTO L., BAJSA N., FABIANIO E., ALTIER A. and ARIAS A. 2002. Inoculation with Pseudomonas fluorescens biocontrol strains does not affect the symbiosis between rhizobia and forage legumes. Soil Biology and Biochemistry 34:545- 548. Pseudomonas fluorescens strains UP61, UP143 and UP148, isolated from Uruguayan soils, have shown the ability to control soil-borne fungal pathogens that cause damping-off in birdsfoot trefoil. In this communication, we study the effect of these strains on the symbiotic efficiency of rhizobia from commercial inoculants in birdsfoot trefoil, alfalfa and white clover. Shoot dry weights and the rate of nodulation by rhizobia were not modified by the presence of Pseudomonas strains, despite antagonistic activity against rhizobia in vitro. Survival of P. fluorescens UP61 and rhizobia on roots in non-sterile soil were not affected by co-inoculation of the selected forage legumes.
DALTON S.J., JACKSON J.A., CHAMBERLAIN D.A., WEBB K.J. and DALE P.J. 1986. Protoplast culture in forage legumes. Genetic Engineering of Plants and Micro-organisms 65-66.
EASON W.R., ABBERTON M, MICHAELSON-YEATES T., PARNISKE M., TEODOSIO H.R., WEBB K.J. and HOOKER J. 1999. Tools for unravelling genetic control of arbuscular mycorrhizal symbiosis in legumes. 9th International Congress Molecular Plant-Microbe Interactions, Amsterdam, The Netherlands. 25-30th July 1999. Abs. no. 11.14.
FITZGERALD D. and FOGARTY P. 1992. Species for Saline Soils on the Northern Tablelands. In: Proceedings 7th Annual Conference of Grassland Society NSW, pp 91-92.
GIBBS M.J., GATEHOUSE J.A. and WEBB K.J. 1990. Lotus corniculatus: a transformation system with Agrobacterium tumefaciens. 20th Meeting of FEBS, Budapest 19-24 August 1990. Abstr. no. P-Th 484.
GIBBS M.J., GATEHOUSE J.A. and WEBB K.J. 1992. Expression and inheritance patterns of the reporter gene (35S-GUS) in the herbage legume Lotus corniculatus. SEB 5-10 April, University of Lancaster, UK. J. Expl. Bot. (suppl.) 43:250 (Abs. No. P10.61) p60.
GIBBS M. and WEBB K.J. 1989. Lotus corniculatus: A transformation system with Agrobacterium tumefaciens. In: 49th Nottingham Easter School. Genetic Engineering of Crop Plants. 17-21 April 1989.
GORDON A.J., SKØT L., MINCHIN F.R., WEBB K.J., WANG T.L., HEDLEY C.L., CRAIG J. and SMITH A.M. 1997. Down regulation of nodule sucrose synthase by mutation and antisense. Nitrogen Fixation Congress Paris.
GORDON A.J., SKØT L., MINCHIN F.R., WEBB K.J., WANG T.L., HEDLEY C.L., CRAIG J. and Lotus literature 71
SMITH A.M. 1998. Down regulation of nodule sucrose synthase by mutation and antisense. IAPTC Jerusalem 14-19th June 1998 Abstract book p185.
HARRIS C.A., BLUMENTHAL M.J., KELMAN W.M. and MCDONALD L. 1997. Effect of cutting height and cutting interval on rhizome development, herbage production and herbage quality of Lotus pedunculatus cv. Grasslands Maku. Australian Journal of Experimental Agriculture, 37, 631-637.
HARRIS C.A., BLUMENTHAL M.J. and SCOTT J.M. 1992. Survey of use and management of Lotus pedunculatus cv ‘Grasslands Maku’ in Eastern Australia. In: Proceedings 6th Australian Society of Agronomy Conference, Armidale, p 545.
HARRIS C.A., BLUMENTHAL M.J. and SCOTT J.M. 1993. Survey of use and management of Lotus pedunculatus cv. Grasslands Maku in eastern Australia. Australian Journal of Experimental Agriculture, 33, 41-47.
HAYASHI M., MIYAHARA A., SATO S., KATO T., YOSHIKAWA M., TAKETA M., HAYASHI M., PEDROSA A., ONDA R., IMAIZUMI-ANRAKU H., BACHMAIR A., SANDAL N., STOUGAARD J., MUROOKA Y., TABATA S., KAWASAKI S., KAWAGUCHI M. and HARADA K. 2001. Construction of a genetic linkage map of the model legume Lotus japonicus using an intraspecific F2 population. DNA Research, 8, 301-310.
HIDALGO S., CALLEJAS L., CAUHÉPÉ M. y OTERO M.J. 2002. Efecto del destete precoz sobre la ganacia de peso de los terneros y sus madres. Investigación Agraria, España. ITEA, 98A, 347-354. [Hidalgo S., Callejas L., Cauhépé M. and Otero M.J. 2002. Effect of the early weaning on the weight gains of the calves and their mothers. Investigación Agraria, España. ITEA, 98A, 347-354. [Spanish]]
HIDALGO L., ERNIE A. y CAUHÉPÉ M. 1998. Digestibilidad de materia seca y contenido de proteína bruta en especies de pastizal de la Pampa Deprimida, Argentina. Investigación Agraria (España), 1,2,3, 1-13. [Hidalgo L., Ernie A. and Cauhépé M. 1998. Dry matter digestibility and protein content in species of the Depressed Pampas grasslands, Argentina. Investigación Agraria (España), 1,2,3, 1-13. [Spanish]]
HUMPHREYS M.O., HAYWARD M.D., MORRIS P. and WEBB K.J. 1997. Applying biotechnology to grass and forage legume breeding; new solutions, new horizons and new challenges. In: Staszewski Z., Mlyniec W. and Osinski R. (Eds) Ecological Aspects of Breeding Fodder Crops and Amenity Grasses. Proceedings of Eucarpia 20th Fodder Crops and Amenity Grass Section Meeting, Plant Breeding and Acclimatisation Institute, Radzikow, Poland, 7-10 October 1996. pp. 283-292.
HUMPHREYS MO., HAYWARD M.D., MORRIS P. and WEBB K.J. 1997. Applying biotechnology to grass and forage legume breeding new horizons, new challenges and new solutions. Eucarpia Fodder Crops and Amenity Grass Section 20th Meeting. October 7-10 1996. Radzikow, Poland. pp. 157-158. (Astract)
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JØRGENSEN B., SKØT L. and WEBB K.J. 1994. T-DNA insertional mutagenesis and chemical mutagenesis in L. japonicus. CEC AMICA Consortium Book of Abstracts.
JØRGENSEN B., SKØT L. and WEBB K.J. 1995. Initial screening of putative T-DNA tagged Lotus japonicus. SEB St Andrews Meeting, 3-7 April 1995. Abs no. 6.65.
JØRGENSEN B., WEBB K.J. and SKØT L. 1994. Evaluation of Lotus japonicus for a T-DNA tagging programme. VIII International Congress on Plant Tissue and Cell Culture. Firenze, 12-17 June 1994. Abs. No. S1-170.
JØRGENSEN B., WEBB K.J. and SKØT L. 1994. New techniques for identifying nitrogen fixation and nodulation genes using T-DNA tagging in Lotus japonicus. Proceedings of the 1st European Nitrogen Fixation Conference Szeged, Hungary. August 29 - September 2. Eds: GB Kiss and G Endre. Officina Press. P79.
JØRGENSEN B., SKØT L. and WEBB K.J. 1994. A study of the flexibility of the transformation system in Lotus japonicus. Lotus Newsletter. Ed: PR Beuselinck USDA-Agricultural Research Service. 25:25-27.
JUAN V., MONTERROSO L., SACIDO M. and CAUHÉPÉ M. 2000. Postburning legume seeding on a Paspalum quadrifarium community in the Flooding Pampas, Argentina. Journal of Range Management, 53, 300-304.
JUAN V., SACIDO M., CAUHÉPÉ M.A. y BALLETO F.I. 1998. Implantación y establecimiento de Lotus tenuis posquema de Paspalum quadrifarium. In: Berreta E. (ed.) Anales: XIV Reunión del grupo técnico regional del Cono Sur en mejoramiento y utilización de los recursos forrajeros del área tropical y subtropical: Grupo Campos. Serie Técnica, INIA Uruguay, No. 94, 69-72. ISBN 9974-38-087-1. [Juan V., Sacido M., Cauhépé M.A. and Balleto F.I. 1998. Installation and establishment of Lotus tenuis after burning of Paspalum quadrifarium. In: Berreta E. (ed.) Anales: XIV Reunión del grupo técnico regional del Cono Sur en mejoramiento y utilización de los recursos forrajeros del área tropical y subtropical: Grupo Campos. Serie Técnica, INIA Uruguay, No. 94, 69-72. ISBN 9974- 38-087-1. [Spanish]]
KELLY R., TEODOSIO R., EASON W., HOOKER J.E. and WEBB K.J. 2001. Physiological markers target key genes in Arbuscular Mycorrhizal clover. ICOM Adelade, Australia July 2001.
KELMAN W.M. and AYRES J.F. 2002. Genetic analysis of seed yield components in birdsfoot trefoil (Lotus corniculatus). In: McComb J.A. (Ed.) Plant Breeding for the 11th Millennium. Proceedings of the 12th Australasia Plant Breeding Conference, Perth WA, 15-20 September 2002, pp 504-506 (Australasian Plant Breeding Assoc Inc).
KELMAN W.M. and AYRES J.F. 2003. Genetic variation for seed yield components in birdsfoot trefoil (Lotus corniculatus L.) Australian Journal of Experimental Agriculture 42, (1) [In press] Lotus literature 73
The persistence of hard-seeded perennial legumes can be facilitated by breeding and management programs that enhance seedling recruitment and maintain plant population density. In northern New South Wales, day-length limits optimal flowering intensity and seed set of the otherwise promising birdsfoot trefoil cultivar, Grasslands Goldie (Lotus corniculatus L.). We studied the feasibility of improving flowering and seed production traits in this cultivar. To measure the extent of genetic variation for seed yield components, half-sib families derived from 45 parent plants collected from a population of cv. Goldie collected from Inverell, New South Wales were grown in the field at Glen Innes NSW and Canberra ACT. Flowering time, flowering intensity and seed yield components (umbels per stem, pods per umbel, pods per stem, seeds per pod and seed mass) were recorded at both sites, while plant weight, height and width were also measured at the Canberra site. There was significant genetic variation among half-sib families and high narrow-sense heritabilities (on a family mean basis) for flowering time (0.73 ± 0.11), flowering intensity (0.66 ± 0.12) and plant height (0.78 ± 0.10). High estimates of narrow-sense heritability for seed yield components indicated that selection advance would be achieved under both sward and spaced plant conditions. There was evidence that selection for prolific flowering in cv. Grasslands Goldie was associated in the progeny populations with an increase in the number of umbels per stem. This component is a readily measured indicator of flowering intensity and should prove useful in developing more prolific and persistent populations from further selection within cv. Goldie and other birdsfoot trefoil populations.
KELMAN W.M. and AYRES J.F. 2003. Genetic variation for seed yield components in birdsfoot trefoil (Lotus corniculatus L.). Australian Journal of Experimental Agriculture, 44, (1) [In press]
KELMAN W.M. and BLUMENTHAL M.J. 1992. Lotus in South-Eastern Australia: Aspects of Forage Quality and Persistence. In: Proceedings 6th Australian Society of Agronomy Conference. The University of New England, Armidale, 10-14 February, pp 460-463.
KELMAN W.M., BLUMENTHAL M.J. and HARRIS C.A. 1992. Evaluation of Lotus pedunculatus and L. corniculatus accessions in south-eastern Australia. Lotus Newsletter, 23, 8-10.
KELMAN W.M., BLUMENTHAL M.J. and HARRIS C.A. 1997. Genetic variation for seasonal herbage yield, growth habit, and condensed tannins in Lotus pedunculatus Cav. and Lotus corniculatus L. Australian Journal of Agricultural Research, 48, 959-968.
KELMAN W.M. and BOWMAN A.M. 1996. Monitoring improvements in seed production and seedling vigour of Greater Lotus (Lotus Pedunculatus Cav.). In: Proceedings 8th Australian Agronomy Conference. The University of Southern Queensland, Toowoomba, 30 January-2 February, pp 341-344.
KIRKHAM K., WEBB K.J., SKØT L. and HUMPHREYS M.O. 1996. Inheritance of transgenes through three generations in an outbreeding legume, Lotus corniculatus. Eucarpia Fodder Crops and Amenity Grass Section 20th Meeting. October 7-10 1996. Radzikow, Poland. p159.
KIRKHAM M., WEBB K.J., SKØT L. and HUMPHREYS M.O. 1997. Inheritance of transgenes in Lotus corniculatus L. Seeds of Progress Conference. 18-19 February 1997. Nottingham, U.84. 74 Lotus literature
Крамина Т.Е. Морфологическая изменчивость Lotus corniculatus L. s. l. в Средней России в связи с систематикой данной группы // Проблемы современной биологии: Тр. 18 науч. конф. мол. ученых биол. фак. МГУ, Москва, 20-24 апр. 1987. Ч. 3 / МГУ. М., 1987. С. 114—116. Деп. в ВИНИТИ 14.09.1987, № 6654-В87. [Kramina T.E. 1987. Morphological variability of Lotus corniculatus L. s. l. in Middle Russia in connection with the systematics of the group // Problems of modern biology: Proc. 18th sci. conf. of young scientists of Biol. Faculty of Moscow State University, Moscow, April 20-24, 1987. Part 3 / MSU. M., 1987. pp. 114-116. Deposited in VINITI 14.09.1987, № 6654-В87 [Russian]].
Крамина Т.Е. Внутривидовая изменчивость Lotus corniculatus L. s. l. на территории Европейской части СССР // Тр. III молодеж. конф. ботаников Ленинграда, Ленинград, апр. 1990. Ч. 1 / Бот. ин-т АН СССР. Л., 1990. С. 116—122. Деп. в ВИНИТИ 14.11.1990, № 5700-В90. [Kramina T.E. 1990. Intraspecies variability of Lotus corniculatus L. s. l. in the European part of the USSR. Proc. III youth conf. of botanists of Leningrad, Leningrad, April 1990. Part 1 / Bot. Inst. of Academy of Science of the USSR. L., 1990. pp. 116-122. Deposited in VINITI 14.11.1990, № 5700-В90 [Russian]].
Крамина Т.Е. Изучение географической изменчивости отдельных морфологических признаков Lotus corniculatus L. s. l. (Leguminosae Juss.) в Европейской России и на сопредельных территориях // Бюл. МОИП. Отд. Биол. 1992. Т. 97, вып. 6. С. 108— 119. [Kramina T.E. 1992. Study of geographical variation of some morphological characters of Lotus corniculatus L. s. l. (Leguminosae Juss.) in European Russia and adjacent territories // Bull. Mosc. Soc. Nat. Biol. Ser., 97, 108-119. [Russian]]. Study of geographical variation pattern of 18 morphological characters of Lotus corniculatus L. s. l. and their relations to variation of macroclimate has been conducted. Polymorphism of the majority of the characters in most populations has been established. Some characters (especially calyx and leaves hair density, length to width ratio for leaflets of a compound leaf, flower size) showed a tendency to clinal variation pattern from north-west to south-east. Mean value correlation between hair density and leaflets size and climatic parameters can suggest their adaptability.
Крамина Т.Е. Изучение морфологической изменчивости в популяциях Lotus corniculatus L. s. l. с применением многомерных методов анализа // Проблемы охраны Дудергофских высот: Тез. IV молодеж. конф. ботаников Санкт-Петербурга (секция сист. и геогр. высших растений). СПб., 1992. С. 32. [Kramina T.E. 1992. Study of morphological variability in populations of Lotus corniculatus L. s. l. by multivariate analysis methods // Problems of conservation of Dudergof heights: Abstr. IV youth conf. of botanists of St.-Petersburg (section of higher plants systematics and geography). SPb., 1992. p. 32 [Russian]].
Крамина Т.Е. Морфологическая и географическая изменчивость Lotus corniculatus L. s. l. (Leguminosae) и проблемы систематики этой группы // Тр. IV молодеж. конф. ботаников Санкт-Петербурга, С.-Петербург, май 1992. Ч. 1 / Бот. ин-т РАН. СПб., 1993. 192 с. Деп. в ВИНИТИ 10.06.1993, № 1621-В93. [Kramina T.E. 1993. Lotus literature 75
Morphological and geographical variability of Lotus corniculatus L. s. l. (Leguminosae) and problems of the systematcs of the group. Proc. IV youth conf. of botanists of St.- Petersburg, St.-Petersburg, May 1992. Part. 1 / Bot. Inst. of RAS. SPb., 1993. Deposited in VINITI 10.06.1993, № 1621-В93 [Russian]].
KRAMINA T.E. 1993. Within- and interpopulation variability of Lotus corniculatus L. species complex in the European Russia and adjacent countries. Proc. IV conf. “Species and its productivity in the distribution area. SPb.: Hydrometeoizdat, 1993. pp. 356-357. // [Материалы IV совещ. “Вид и его продуктивность в ареале”. СПб.: Гидрометеоиздат, 1993. С. 356—357.]
KRAMINA T.E. 1994. Lotus corniculatus L. complex in the European Russia. In: Beuselink P.R. and Roberts C.A. (Eds) Proc. First Intern. Lotus Symposium, 22-24 March 1994. Missouri Botanical Gardens. St. Louis, Missouri, USA / Univ. of Missouri-Columbia, 1994. pp. 25-26.
Крамина Т.Е. Морфологическая изменчивость в популяциях комплекса Lotus corniculatus L. s. l. на юге европейской части России и проблема межвидовых границ в этой группе // IX Московское совещание по филогении растений: Материалы / Под ред. чл.-корр. РАН, проф. В.Н.Тихомирова. - М.: Изд. секции ботаники Моск. о-ва испытателей природы и каф. высших растений биол. ф-та Моск. ун-та, 1996. С. 74—76. [Kramina T.E. 1996. Morphological variation in populations of Lotus corniculatus L. s. l. complex in the south of the European part of Russia and the problem of interspecific limits in this group. In: Tikhomirov V.N. (ed.). Proceedings IXth Moscow conf. of plants phylogeny. M.: 1996. pp. 74-76 [Russian]].
KRAMINA T.E. 1997. Variability of some morphological characters of Lotus corniculatus complex in the European Russia and Ukraine // In: Smets E., Ronse Decraene L.P. and Robbrecht E. (eds) 13th Symposium Morphology, Anatomy and Systematics: Program. and abstr. / Meuse: Nat. Bot. Garden of Belgium, 1997. (Scripta Botanica Belgica; Vol. 15). p. 97.
Крамина Т.Е. Морфология пыльцы некоторых представителей рода Lotus (Fabaceae) // Проблемы ботаники на рубеже XX-XXI веков: Тез. докл., предст. II (X) съезду Рус. бот. о-ва (26-29 мая 1998 г., Санкт-Петербург). Т. 1. СПб.: Изд. БИН РАН, 1998. С. 47—48. [Kramina T.E. 1998. Pollen morphology of several representatives of Lotus (Fabaceae) // Problems of botany in the border of XX-XXI centuries: Abstr. of II (X) Congress of Rus. Bot. Society (May 26-29, 1998, St.-Petersburg). T. 1. SPb.: Bot. Inst. RAS, 1998. pp 47-48. [Russian]].
KRAMINA T.E. 1999. A contribution to the taxonomic revision of the Lotus corniculatus complex (Leguminosae, Loteae) in the European part of the former USSR. Syst. Geogr. Pl., 68, 265-279. The variability in twenty morphological characters was studied in 54 local populations of Lotus corniculatus L. complex in the European part of Russia and adjacent areas. Chromosome numbers 76 Lotus literature were counted for samples taken from 15 locations. A tendency towards a clinal variation has been demonstrated for several characters, especially for leaflets indexes, indumentum density and flower length. Correlations between some morphological characters (leaflets size, indumentum density and others) and climate parameters have been revealed. On the basis of a factor analysis, four species are distinguished in the complex occurring in the study area: L. krylovii Schischk. et Serg. (2n=12), L. tenuis Waldst. et Kit. ex Willd. (2n=12), L. ucrainicus Klok. (2n=12), and L. corniculatus L. (2n=24).
Крамина Т.Е. Значение признаков строения семян для систематики секции Lotus рода Lotus L. (Leguminosae - Papilionoideae - Loteae) // X Московское совещание по филогении растений: Материалы. под ред. проф. Л.И.Лотовой и проф. А.П.Меликяна. - М.: Изд. секц. ботаники Моск. о-ва испытателей природы и каф. морфол. и систематики высш. раст. Моск. гос. ун-та им. М.В.Ломоносова, 1999. С.90—93. [Kramina T.E. 1999. The significance of seed characters in the systematics of the genus Lotus L. sect. Lotus (Leguminosae - Papilionoideae - Loteae) // In: Lotova L.I. and Melikian A.P. (eds.). Proceedings of the X Moscow Conf. on Plants Phylogeny, M.: MSU, pp 90-93. [Russian]].
Крамина Т.Е. Таксономическая ревизия секции Lotus рода Lotus L. (Leguminosae) на территории Европейской России и сопредельных государств. — Моск. гос. ун-т. Дисс. на соиск. уч. степ. канд. биол.наук. М., 1999. [Kramina T.E. 1999. Taxonomic revision of the section Lotus (genus Lotus L., Leguminosae) in the European Russia and adjacent countries. Moscow State Univ., Ph.D. Thesis, Mocsow [Russian]].
Крамина Т.Е. Новый вид рода Lotus L. (Leguminosae) из группы L. corniculatus L. // Бюл. МОИП. Отд. Биол. 2000. Т. 105, вып. 1. С. 35—40. [Kramina T.E. 2000. A new species of Lotus L. (Leguminosae) from L. corniculatus L. group // Bull. MOIP. Sect. Biol., 105, 35-40 [Russian]]. A new species, Lotus stepposus Kramina, distributed in steppe areas of European Russia (and partly in Siberia), Ukraine, Moldova, Romania, Kazakhstan and Turkey is described. The species is characterized by diploid chromosome number 2n=12. Its relations to L. tenuis Waldst. et Kit. ex Willd. (=L. glaber Mill.) and L. corniculatus L. s. str. are discussed.
KRAMINA T.E. 2001. A taxonomic study of the genus Lotus (Papilionoideae, Loteae): generic limits, infrageneric division and the position of some critical species // The Fourth Intern. Legume Conf., 2—6 July 2001, Austral. Nat. Univ., Canberra, Australia: Program and Abstracts. Legume Down Under. pp 46—47.
Крамина Т.Е. Использование корреляций признаков в микросистематике // Междунар. науч. конф. по систематике высших растений, посвященная 70-летию со дня рождения чл.-корр. РАН, проф. В.Н.Тихомирова (Москва, 28-31 января 2002 г.): Тез. докл. — М.: Изд-во Центра охраны дикой природы, 2002. — С.58—59. [Kramina T.E. 2002. The use of feature correlations in microsystematics // International Sci. Conf. on the systematics of higher plants dedicated to 70th anniversary of Associate Academician, Prof. V.N.Tikhomirov’s birthday (Moscow, 28-31 January 2000): Abstr. - M.: BCC-Press. pp 58-59 [Russian]].
Lotus literature 77
Крамина Т.Е., Барыкина Р.П. Сравнительно-анатомический анализ некоторых лядвенцев Японии // Тез. докл. II Междунар. конф. по анат. и морфол. растений. Санкт-Петербург, 14-18 окт. 2002 г. СПб., 2002. С.58-59. [Kramina T.E. and Barykina R.P. 2002. Comparative anatomical analysis of several species of Lotus from Japan // Abstr. II International Conf. Anat. Morphol. of Plants. St.-Petersburg, October 14-18, 2002. SPb. pp.58-59 [Russian]]. Anatomical structure of vegetative organs (i.e. stems, leaves, and roots) in Lotus corniculatus L. var. japonicus Regel, L. corniculatus var. corniculatus, L. krylovii Schischk. et Serg. and discovered in Miyako-jima island in the south of Japan L. sp. nova has been examined. The first two taxa are perennials and the last two are predominantly annuals. Some difference in microstructure of vegetative organs between studied taxa has been revealed. The roots of L. krylovii and L. sp. nova are characterized by thin cork with small number of layers and by the absence of reserve starch that is connected with their annual life cycle. Leaves of L. sp. nova distinguish from those of other studied taxa by the highest number of mesophyll layers and the highest ratio of palisade to spongy mesophyll thickness. L. corniculatus var. japonicus has no additional bundles in stems that are usual for other taxa.
KRAMINA T.E. and SOKOLOFF D.D. 1991. Lotus roudairei Bonnet and taxonomic relationships between African and north American species of the tribe Loteae (Papilionaceae) // Adansonia. Ser. 3, 19, 321-328. A comparative analysis of morphological features in NW African Lotus roudairei, N. American Lotus sect. Simpeteria, and American Lotus sect. Microlotus (gen. Acmispon s. str.) was carried out. According to the data obtained, these three taxa seemed not to form the distinct genus or subgenus Acmispon sensu P. Lassen (1986). A new section Pseudosimpeteria with a single species, L. roudairei, is described within the Old World Lotus subgen. Lotus. The relationships between Old World and New World Loteae are briefly discussed.
KRAMINA T.E. and SOKOLOFF D.D. 1997. Lotus roudairei Bonnet and taxonomic relationships between African and North American species of the tribe Loteae (Papilionaceae). Adansonia, sér. 3., 19, 321-328.
KRAMINA T.E. and SOKOLOFF D.D. 1999. Taxonomic bearing of stylodium tooth in the genus Lotus (Papilionaceae) with special reference to Lotus creticus L. Feddes Repert., 110, 521-527. Stylodium morphology in 62 species of Lotus is studied. In Lotus creticus, a stylodium tooth is detected for the first time. Therefore, L. creticus should be transferred from subgenus Edentolotus into subgenus Pedrosia. L. cytisoides and L. longisiliquosus which were often indicated as scarcely distinguishable from L. creticus, are retained in the subgenus Edentolotus and can be easily separateв from each other if L. creticus is excluded from the subgenus.
Крамина Т.Е., Соколов Д.Д. Kebirita — новый род Leguminosae—Loteae из северо- западной Африки // Бюл. МОИП. Отд. Биол., 2001. Т. 106, вып. 3. С. 58—63. [Kramina T.E. and Sokoloff D.D. 2001. Kebirita - a new genus of Leguminosae— Loteae from North-Western Africa. Bull. Moscow Soc. Natur. Biol. Ser., 106, 58-63. [Russian]]. A new monotypic genus Kebirita Kramina et D.D.Sokoloff (= Lotus sect. Pseudosimpeteria Kramina et D.D.Sokoloff) is decribed from NW Africa. These are perennial herbs without rhizomes with pinnate leaves with 3-6 usually alternate leaflets and glandular stipules. They possess one-flowered umbels on short peduncles. On the peduncle there are two opposite small scaly leaves, one of them is the flower 78 Lotus literature covering leave. There are no leaves with normally developed blade on the peduncle. Ovules micropylae alternates. Pollen grains with 3 apertures. The single species of the genus, Kebirita roudairei (Bonnet) Kranima et D.D.Sokoloff (= Lotus roudairei Bonnet), is distributed in arid areas of Algeria, Tunisia, Morocco, Mauritania and West Sahara.
KRAMINA T.E. and SOKOLOFF D.D. 2003. On section Erythrolotus of the genus Lotus and related taxa. Bull. Moscow Soc. Natur. Biol. Ser., 108, N 5. [Russian](in press). Sect. Erythrolotus is accepted in narrow sense, comprising only L. conibricensis Brot., the species which was selected as a lectotype of the section. L. conimbricensis share with members of sect. Lotus mainly Mediterranean distribution and base chromosome number x=6. L. conimbricensis is characterised by long and narrow, incurved and tardily dehiscent fruit. The majority of species traditionally included in section Erythrolotus is placed now in the section Heinekenia Webb et Berth. A new section, Chamaelotus is described to include three annual species with shortened peduncles and small pink or red flowers. A new combination at sectional level is made, namely sect. Benedictella (Maire) Kramina et D.D. Sokoloff. The section Benedictella is monotypic and includes L. benoistii (Maire) Lassen from Morocco.
Крамина Т.Е., Соколов Д.Д. Дисткретность и континуальность таксонов и их диагностических признаков в систематике рода Lotus L. (Leguminosae) // XI Международное совещание по филогении растений. Тезисы докладов (Москва, 28- 31 января 2003 г.) / Под ред. В.С.Новикова, А.К,Тимонина и А.В.Щербакова. — М.: Изд-во Центра охраны дикой природы, 2003. С. 54-55. [Kramina T.E. and Sokoloff D.D. 2003. Classification and evolution of the genus Lotus L. (Leguminosae) // XI International plant phylogeny symposium. Abstracts (Moscow, 28-31 January 2003) / V.S.Novikov, A.K.Timonin, A.V.Shcherbacov (eds.). M.: BCC-Press, 2003. p. 54-55 [Russian]].
Крамина Т.Е., Тихомиров В.Н. Внутрипопуляционная изменчивость отдельных морфологических признаков Lotus corniculatus L. s. l. (Leguminosae) на территории европейской части СССР // Бюл. МОИП. Отд. Биол. 1991. Т. 96, вып. 6. С. 117—126. [Kramina T.E. and Tikhomirov V.N. 1991. Within populational variability of some morphological characters of Lotus corniculatus L. s. l. (Leguminosae) in the European part of the USSR. Bull. Mosc. Soc. Nat. Biol. Ser., 96, 117-126. [Russian]]. The study of variability limits and estimation of diagnostic values of 18 morphological characters, being usually referred to in determination of taxa within the Lotus corniculatus L. s. l. complex from the European part of the USSR have been carried out, basing on the data of samples from 22 local populations. Each character was shown to be of overlapping variability within the adjacent populations, representing the argument against the independence of the studied races (L. ucrainicus Klok., L. ruprechtii Min., L. balticus Min., L. zhegulensis Klok., L. callunetorum (Juxip.) Min., and L. corniculatus L. s. str.). The received data require further examination with the help of multidimentional methods.
LATERRA P. 1997. Post-burn recovery in the flooding Pampa: impact of an invasive legume. J. Range Manage, 50, 274-277.
LATERRA P. and BAZZALO M.E. 1999. Seed to seed allelopathic effects between two invaders of burned grasslands in the Flooding Pampa. Weed Research, 39, 297-308. Lotus literature 79
The establishment of Lotus tenuis Waldst et Kit. (narrowleaf birdsfoot trefoil) can interfere with colonisation by Carduus acanthoides L. (musk thistle) during the early postburn recovery of Flooding Pampa grasslands. The purpose of this research was to determine the potential role of L. tenuis seeds as source of allelopathic compounds involved in that interaction. Imbibed seeds of L. tenuis and aqueous leachates from them were bioassayed for their ability to inhibit germination and seedling growth of C. acanthoides, both on sterilised filter paper and on pasteurized soil as substrata. Germination or emergence of C. acanthoides were inhibited and root length was reduced on filter paper or soil, by both the presence of L. tenuis seeds and by their leachate, at densities of L. tenuis near the maximum values observed in the field. Germination and seedling growth of C. acanthoides were less affected by the presence of L. tenuis seeds than by the addition of their leachate, and the presence of L. tenuis seeds or their leachate showed stronger effects on emergence of C. acanthoides from soil than on its germination on filter paper. Methods applied for leachate sterilization, ultrafiltration or autoclaving, did not modify C. acanthoides responses. Neither the germination rate nor the root length of C. acanthoides seedlings were affected by solutions of polyethylene glycol with similar osmolarity to the leachates. We conclude that the release of inhibitory substances onto filter paper and into pot soil from imbibed L. tenuis seeds would be the mechanism responsible for the observed effects.
LATERRA P. and SOLBRIG O.T. 2001. Dispersal strategies, spatial heterogeneity and colonization success in fire-managed grasslands. Ecological Modelling, 139, 17-29. Interactions between fire regime, dispersal strategies and patch structure were examined as key issues for the management of floristic composition of grasslands, through a model that simulates the population dynamics of two competing fire-cued and non-sprouting species. The model describes a heterogeneous environment composed by several patches of grassland, only related by seed dispersal. The last burn date at each patch determines the accumulation level of fuel-biomass provided by a third, dominant species, which in turn controls for the exclusion rate of both colonizer species. The population dynamics of both species was approached following density-dependent models and parameterized for two opposite dispersal strategies: low spatial and high temporal dispersion of seeds (type 1), high spatial and low temporal dispersion of seeds (type 2). Only under the most variable scenarios (when non-synchronous and irregular fire regimes were combined with a proportion of patches (p) with initially depleted seed banks) did the relative success of dispersal strategies vary with the length of the fire-free period. Irrespective of p, smaller interval lengths favored the postburn density of the strategy 1. Strategy 2 was favored over strategy 1 when the fire-free interval increased, such difference being maximum for intermediate p values. These general tendencies agree with those observed from a reference system: the Flooding Pampa grasslands dominated by Paspalum quadrifarium where short no-fire intervals promote the postburn abundance of a type 1 species (Lotus tenuis) over two type 2 species (Carduus acanthoides and Cirsium vulgare) while for long fire-free intervals the opposite is true. These results suggest that frequency, time since last burn, and burning synchrony are useful components of a fire regime to take advantage of variation in dispersal strategies.
LATERRA P., VIGNOLIO O.R., HIDALGO L.G., FERNÁNDEZ O.N., CAUHÉPÉ M.A. y MACEIRA N.O. 1998. Dinámica de pajonales de paja colorada (Paspalum spp) manejados con fuego y pastoreo en la pampa deprimida argentina. Ecotrópicos, 11, 11-149. [Laterra P., Vignolio O.R., Hidalgo L.G., Fernández O.N., Cauhépé M.A. and Maceira N.O. 1998. Dynamics of grasslands of red straw (Paspalum spp) managed with fire and grazing in the Argentinean depressed pampas. Ecotrópicos, 11, 11-149. [Spanish]]
LATERRA P., VIGNOLIO O.R., LINARES P., GIAQUINTA A. and MACEIRA N.O. 2003. Cumulative effects of fire on the structure and function of a tussock pampa grassland. Journal of Vegetation Science, 14, 43-54. 80 Lotus literature
In order to test for cumulative effects of fire on Paspalum quadrifarium-dominated grasslands (“pajonal”), we analyzed the impact of single and repeated fires on the community structure and post- fire recovery of canopy after a final, simultaneous fire event. Nine plots were defined within a homogeneous pajonal stand, and treatments of low (LF), medium (MF) and high frequency (HF) of fire were defined by the application of one, two or four cold-season burnings, respectively, along a six-year period. Both burned and unburned plots were exposed to grazing by cattle during the summer following the first and the third years of that period. High cattle preference for burned sites conditioned fire temperature and vegetation responses to the following burning events. Cumulative effects between successive burning events were observed on the cover of basal area of the dominant and other sprouter species, the cover and thickness of the litter layer, the seed bank size of the principal recruiter species, and the floristic composition. While light interception by the canopy was positively related to fire frequency during the early growth season, further growth of P. quadrifarium determined a greater light interception in LF than in MF and HF. These patterns of light interception were associated with a faster occupation of the between-tussock areas by opportunistic species in plots subjected to frequent fires (HF and MF) than in plots with low fire frequency (LF), and a more lasting regrowth of P. quadrifarium in the LF plot than in the HF ones. High fire frequencies reduced the dominance of P. quadrifarium. Percent of species classified as subordinated graminoids or forbs did not vary among treatments. However, the abundance of different forb species was differentially favored by contrasting frequencies of fire, describing some coarse relationships between their specific responses and their dispersal strategies.
LEPEK V.C., D’ANTUONO A.L., TOMATIS P.E., UGALDE J.E., GIAMBIAGI S., and UGALDE R.A. 2002. Analysis of Mesorhizobium loti glycogen operon: effect of phosphoglucomutase (pgm) and glycogen synthase (glgA) null mutants on nodulation of Lotus tenuis. Molecular Plant-Microbe Interactions 15, 368-375.
LEWIS M., SKØT L. and WEBB K.J. 1993. The manipulation of soil microbe populations via transgenic plants. Molecular Biology and Evolution. UCW-IGER Aberystwyth UK 4th-6th January 1993.
MARTINS E.S. and SOKOLOFF D.D. 2003. Lotus robsonii E.S. Martins & D.D. Sokoloff, sp. nov. In: Flora Zambesiaca. Vol. 3. Pt. 7. Kew: Royal Botanic Gardens, pp. 3–4. A new species of Lotus is described from Malawi. It differs from related species by leaves with 3, not 5 leaflets.
MCLAUGHLIN B.D. and CLARKE J.B. 1989. Lotus for pasture and seed production. NSW Agriculture Agfact P2.5.30, first edition.
MONTERROSO L., JUAN V., SACIDO M. y CAUHÉPÉ M.A. 1998. Incidencia y severidad de patógenos en Lotus tenuis durante su implantación y establecimiento en pajonales de paja colorada (Paspalum quadrifarium). Revista Latinoamericana de Fitopatología, 33, 224- 227. [Monterroso L., Juan V., Sacido M. and Cauhépé M.A. 1998. Disease incidence and severity in Lotus tenuis during their installation and establishment in grasslands of red straw (Paspalum quadrifarium). Revista Latinoamericana de Fitopatología, 33, 224-227. [Spanish]]
MORRIS, P., CARRON T.R., ROBBINS M.P. and WEBB K.J. 1993. Distribution of condensed Lotus literature 81
tannins in flowering plants of Lotus corniculatus var japonicus and tannin accumulation by transformed root cultures. LOTUS Newsletter.
MORRIS P., ROBBINS M.P. and WEBB K.J. 1994. Elicitation of phytoalexins in hairy root culutres of Lotus cornciculatus, Trifolium repens and Cicer arietinum. SEB Univeristy College of Swansea, UK. 11-15 April 1994. [P3.1]
MORRIS P., SKØT L., WEBB K.J., JØRGENSEN B. and ROBBINS M.P. 1998. Application of Biotechnology to Lotus breeding. In: Trefoil: Beuselinck P., Nelson J. and Hoveland C. (eds) The Science and Technology of Lotus. American Society of Agronomy and Crop Science. (ASA-CSSA-SSSA).
MORRIS P., WEBB K.J. and ROBBINS M.P. 1990. Phenyl-propanoid metabolism in Agrobacterium rhizogenes transformed cultures of herbage legumes. VIIth International Congress on Plant Tissue and Cell Culture, Amsterdam 24-29 June 1990. Abstr. no. C5-40
MORRIS P., WEBB K.J., ROBBINS M.P. and SKØT L. 1994. Applications of tissue culture, molecular biology and genetic manipulation in Lotus research. Proceedings First International Lotus Symposium, 22-24 March 1994, St Louis, Missouri, USA. Ed: P Beuselinck. pp. 103-108.
MORRIS P., WEBB K.J., ROBBINS M.P., SKØT L. and JØRGENSEN B. 1999. Application of Biotechnology to Lotus Breeding. In: Beuselinck P., Nelson J. and Hoveland C. (eds) Trefoil: The Science and Technology of Lotus. CSSA Special Publication 28. pp. 199-228. Crop Science Society of America and American Society of Agronomy (ASA-CSSA), Madison, USA. ISBN 089118550X.
NICHOLSON M. [WEBB K.J., SKØT L., JØRGENSEN B. and MIZEN S.] 1998. GUS activity. Microscopical detection of gene activity in legume roots is one step closer to cloning nitrogen fixation genes. Microscopy and Imaging News 150 April p5.
NICHOLSON M.M., JØRGENSEN B., MIZEN S., SKØT L. and WEBB K.J. 1996. Tagging nodulation and nitrogen fixation genes in Lotus japonicus. Molecular Plant-Microbe Interactions. Knoxville, Tennessee, USA. 14-19 July 1996. Abs. No. 31.
NICHOLSON M.M., JØRGENSEN B., MIZEN S., SKØT L. and WEBB K.J. 1996. Tagging nodulation and nitrogen fixation genes in Lotus japonicus. SEB, Dublin, Eire. 22-26 July 1996. Abstract no P12.
NICHOLSON M.M., JØRGENSEN B., MIZEN S., SKØT L. and WEBB K.J. 1997. Tagging nodulation and nitrogen fixation genes in Lotus japonicus. PMBII Final Meeting, 12-14th February 1997. Norwich, U.K. Abs. No. 5.
NICHOLSON M., WEBB K.J. and SKØT L. 1996. Nitrogen fixation genes - falling into the promoter trap. Conference of Plant Science Wales 9-10 January 1996. IGER, Aberystwyth, UK. 82 Lotus literature
NISBET G.S. and WEBB K.J. 1990. Transformation in legumes. In: Bajaj YPS (Ed) Biotechnology in Agriculture and Forestry Vol 10. Legumes and Oilseed Crops I. Springer- Verlag, Berlin. pp. 38-48.
NOVERO M., FACCIO A., GENRE A., STOUGAARD J., WEBB K.J., MULDER L., PARNISKE M. and BONFANTE P. 2002. Dual requirement of the LjSym4 gene for mycorrhizal development in epidermal and cortical cells of Lotus japonicus roots. New Phytologist, 154 (3), 741-749.
OLMOS F. 2001. Mejoramiento de pasturas con lotus en la región noreste. INIA Uruguay, Serie Técnica INIA Tacuarembó, No. 124, 48 p. [Olmos F. 2001. Improvement of natural grasslands with lotus in the northeast region. INIA Uruguay, Serie Técnica INIA Tacuarembó, No. 124, 48 p. [Spanish]]. In the period 1992-1996 four experiments were carried out to identify forage legumes (Lotus, Trifolium and Ornithopus) adapted to grow in the natural pastures of the northeast region of the country. Lotus was the genera better adapted when oversown in the natural pastures. The soils generally have a low P content compared to the legume requirements. In the fourth growing season 5 and 75 % of the -1 improved pasture was Lotus corniculatus L. when 0 and 80 kg of P2O5 ha were applied each year. -1 LAI of the legume increased from 1.5 to 5.0 as the P2O5 increased from 0 to 120 kg ha . There was a strong correlation between seed production and seedling recruitment in the following season. In the first growing season 1 plant dm-2 was recorded, while in the third recording period 3 and 6.4 plants dm- 2 -1 were recorded when 0 and 120 kg ha of P2O5 were applied respectively. Based on plant survival and fecundity a model using a Leslie matrix was developed. The model emphasize the need of seedling recruiment every year to keep the introduced population in natural pasture.
OREA A., PAJUELO P., PAJUELO E., MÁRQUEZ A.J. and ROMERO J.M. 2001. Characterisation and expression studies of a root cDNA encoding for ferredoxin-nitrite redcutase from Lotus japonicus. Physiologia Plantarum, 113, 193-202.
OREA A., PAJUELO P., PAJUELO E., QUIDIELLO C., ROMERO J.M. and MÁRQUEZ A.J. 2002. Isolation of photorespiratory mutants from Lotus japonicus deficient in glutamine synthetase. Physiologia Plantarum, 115, 352-361.
ORTEGA E. and LATERRA P. 2003. Fire-cued colonization of a Flooding Pampa grassland by thistle species: remnant litter and interference effects. Applied Vegetation Science, 6, 35- 44. Winter and Spring burnings constitute a frequent management tool of native grasslands dominated by the bunch grass Paspalum quadrifarium (hereafter "pajonal stands") in the Flooding Pampa of Argentina. In addition to increasing the primary productivity and the nutritious quality of the regrowth, this practice favours the establishment of opportunistic species, especially Lotus tenuis ("lotus"), Cirsium vulgare and Carduus acanthoides ("thistles"). The aims of the present study were to assess the effects of burning and those of the remnant litter on L. tenuis and thistle recruitment, as well as the effects of L. tenuis density on the emergence, survival and flowering of thistles. Two field experiments were carried out. In the first, a completely randomised factorial design with occurrence of L. tenuis and fire was used and, in the second, a completely randomised design with L. tenuis seed density as the only factor. Lotus tenuis and thistle recruitment within the pajonal stand was absolutely dependent on Lotus literature 83 fire. While the presence of L. tenuis and thistles at small spatial scale depended on nearly complete combustion of litter, early presence of thistle seedlings in denuded microsites showed a negative association with the proximity of L. tenuis seedlings. By the end of the first post-fire growing season, the survivorship of established thistles was linearly reduced with the sowing density of L. tenuis. Keeping a significant litter coverage as well as favouring the presence of a high density of L. tenuis in the pre-fire seed bank would constitute management measures aimed to preclude thistle invasion of burned pajonal stands, thus reducing the dependency on chemical control.
ORTEGA E., VERGARA P., VIGNOLIO O. y LATERRA P. 2000. Efectos de la densidad de Lotus tenuis sobre la emergencia, supervivencia y cobertura de Cirsium vulgare. Ecología Austral 10: 143-149. [Ortega E., Vergara P., Vignolio O. and Laterra P. 2000. Effects of the density of Lotus tenuis on the establishment, survival and covering of Cirsium vulgare. Ecología Austral 10: 143-149. [Spanish]] After burning for raising the stocking rate, the Paspalum quadrifarium-dominated grasslands of the Flooding Pampa become invaded by a forage legume, Lotus tenuis, and /or by Cirsium vulgare between other weed species. Aiming to compare the relative impact of L. tenuis on pre and post-emergence stages of C. vulgare, the emergence, survivorship and coverage of C. vulgare, were evaluated in response to four sowing densities of L. tenuis (0,0; 0,25; 0,5 and 1,0 seeds / cm²) and two spatial patterns of C. vulgare (15 uniformly distributed seeds within a circle of 2,5 cm diameter and 15 evenly spaced seeds on a circle of 14 cm diameter), by using a factorial design under greenhouse conditions. The emergence of C. vulgare lineally declined with increasing densities of L. tenuis, independently of its spatial patterns of sowing. While the emergence of C. vulgare was reduced up to 40% by the presence of L. tenuis, the effects of this species on the seedling mortality of the emerged seedlings reached up to 270% of increment. In contrast with the emergence , which was mostly affected by the maximum densities of L. tenuis, the final coverage of C. vulgare was similarly reduced for the presence of L. tenuis irrespective of its density. This results confirm the occurrence of pre-emergent effects of L. tenuis seeds on C. acanthoides seeds, as previously detected under laboratory conditions, even under potentially adverse conditions for the activity of allelopathic compounds.
PACIOS-BRAS C., SCHLAMAN H.R.M., BOOT K., ADMIRAAL P., MATEOS LANGERAK J., STOUGAARD J. and SPAINK H.P. 2003. Auxin distribution in Lotus japonicus during root nodule development. Plant Molecular Biology, 52, 1169-1180. http://www.kluweronline.com/issn/0167-4412/current For this work, Lotus japonicus transgenic plants were constructed expressing a fusion reporter gene consisting of the genes β-glucuronidase (gus) and green fluorescent protein (gfp) under control of the soybean auxin-responsive promoter GH3. These plants expressed GUS and GFP in the vascular bundle of shoots, roots and leafs. Root sections showed that in mature parts of the roots GUS is mainly expressed in phloem and vascular parenchyma of the vascular cylinder. By detecting GUS activity, we describe the auxin distribution pattern in the root of the determinate nodulating legume L. japonicus during the development of nodulation and also after inoculation with purified Nod factors, N- naphthylphthalamic acid (NPA) and indoleacetic acid (IAA). Differently than white clover, which forms indeterminate nodules, L. japonicus presented a strong GUS activity at the dividing outer cortical cells during the first nodule cell divisions. This suggests different auxin distribution pattern between the determinate and indeterminate nodulating legumes that may be responsible of the differences in nodule development between these groups. By measuring of the GFP fluorescence expressed 21 days after treatment with Nod factors or bacteria we were able to quantify the differences in GH3 expression levels in single living roots. In order to correlate these data with auxin transport capacity we measured the auxin transport levels by a previously described radioactive method. At 48 h after inoculation with Nod factors, auxin transport showed to be increased in the middle root segment. 84 Lotus literature
The results obtained indicate that L. japonicus transformed lines expressing the GFP and GUS reporters under the control of the GH3 promoter are suitable for the study of auxin distribution in this legume.
PAJUELO P., PAJUELO E., OREA A., ROMERO J.M. and MÁRQUEZ J.A. 2002. Influence of plant age and growth conditions on nitrate assimilation in roots of Lotus japonicus plants. Functional Plant Biology, 29, 485-494
PARNISKE M., COOMBER S., KISTNER C., MULDER L., PITZCHKE A., STOUGAARD J., SZCYGLOWSKI K., WEBB K.J. and STRACKE S. 2000. Plant genetics of symbiosis. Second International Conference on the Model Legume Lotus japonicus. Norwich, U.K. 24-28th June 2000. Abs. No. S27.
PARNISKE M., COOMBER S., KOYAMA M., WEBB K.J., SZCZYGLOWSKI K., SANDAL N. and STOUGAARD J. 1999. Mycorrhiza mutants of Lotus japonicus. 9th International Congress Molecular Plant-Microbe Interactions, Amsterdam, The Netherlands. 25-30th July 1999. Abs. no. 11.10.
PARNISKE M., WEBB K.J., MARTINI I., GENRE A.and BONFANTE P. 1998. Analysis of Gigaspora and Glomus infection sites in symbiotic mutants of Lotus japonicus. International Congress of Mycorrhiza. Upsala, Sweden. Abs no.231.
PARNISKE M., WEBB K.J., MARTINI I., GENRE A., BONFANTE P., SZCYGLOWSKI K., DE BRUIJN F., SCHAUSER L., SANDAL N. and STOUGAARD J. 1998. Mycorrhiza mutants of Lotus japonicus. First International Lotus japonicus Workshop. 20-22nd November 1998, University of Aarhus, Denmark.
PARNISKE M., WULFF B., HUGHES D. and WEBB K.J. 1996. Mycorrhizal mutants of Lotus japonicus. Emerging Model Legume Systems: Tools and Recent Advances. Knoxville, Tennessee July 12-14, 1996. Abs. no. 15.
PARUELO J.M., OESTERHELD M., DI BELLA C.M., ARZADÚN M., LAFONTAINE J., CAUHÉPÉ M. and REBELLA C.M. 2000. Estimation of primary productivity of subhumid rangelands from remote sensing data. Appl. Vegetation Science, 3, 188-195.
PEDROSA A., SANDAL N., STOUGAARD J., SCHWEIZER D.and BACHMAIR A. 2002. Chromosomal map of the model legume Lotus japonicus. Genetics, 161, 1661-1672.
PÉREZ C., DE LA FUENTE L., ARIAS A. y ALTIER A. 2001. Uso de Pseudomonas fluorescentes nativas para el control de enfermedades de implantación en Lotus corniculatus L. Agrociencia, V, 41-47. [Pérez C., De La Fuente L., Arias A. and Altier A. 2001. Use of native fluorescent Pseudomonas for controlling seedling diseases of Lotus corniculatus L. Agrociencia, V, 41-47. [Spanish]] Seedling diseases caused by Pythium spp. are one of the main constraints for pasture productivity and persistence. During 1996, 1997 and 1998, experiments were conducted under field conditions to Lotus literature 85 evaluate the ability of three strains of native fluorescent Pseudomonas (UP61, UP143, UP148) that produce HCN, siderophores and antibiotics to suppress seedling diseases on Lotus corniculatus. The experimental design was a Randomized Complete Block with five replications. One hundred viable seeds were sown per plot, previously treated as follows: Mesorhizobium loti B816 with each one of the strains of Pseudomonas; M. loti B816 without Pseudomonas; M. loti B816 + metalaxyl (fungicide) sprayed on the soil. Favorable conditions for the development of damping-off only occurred during 1996 (rainfall and low temperatures). That year, despite differences were not statistically significant, the treatments inoculated with Pseudomonas had higher establishment percentage and higher dry matter production per plot than the control without Pseudomonas. In 1997 and 1998, the inoculation with Pseudomonas did not increase the number of established plants but induced an increase on dry matter production per plot suggesting the possible occurrence of an effect on plant growth promotion. The observed trends indicate that the experiments should be repeated in order to validate the practical implementation of this management strategy, under the various soil and environmental conditions and microbial community structures found in Uruguay.
PEREZ N.B., DALL'AGNOL M., DALBOSCO M., BANGEL E.V., MAEYER J. e OLIVEIRA A.M.R. 2003. Caracterização e seleção de estirpes de Rizobium spp em plantas de cornichão. In: Reuniao Anual da SBZ, 2003, Santa Maria. Anais da SBZ. Editora SBZ- CD ROM, 40, p.1-5. [Perez N.B., Dall'Agnol M., Dalbosco M., Bangel E.V., Maeyer J. and Oliveira A.M.R. 2003. Characterization and selection of birdsfoot trefoil Rhizobium isolates. In: Reuniao Annual da SBZ, 2003, Santa Maria. Anais da SBZ. Editora SBZ- CD ROM, 40, p.1-5. [Portuguese]] The objective of this work was to select rhizobium isolates for birsdfoot trefoil, ‘Lotus corniculatus’, by collecting nodules on plants on four cities of Rio Grande do Sul. Through a collaborative work between MIRCEN-FEPAGRO and UFRGS it was possible to characterize the collected material by using immuno-specific serum. The isolated characterization has revealed the existence of antigenic variability. After the characterization the isolated were tested in a complete block design experiment in the greenhouse with three replicates. The results indicated that there is variability for the isolates and that it is possible to select isolated more efficient than the ones that are recommended.
PERRY J.A., WANG T.L., WELHAM T.J., GARDNER S., PIKE J.M., YOSHIDA S. and PARNISKE M. 2003. A TILLING reverse genetics tool and a web-accessible collection of mutants of the legume Lotus japonicus. Plant Physiology, 131, 866-871.
PIPPOLO L. 1998. Incidencia de la avispita Bruchophagus platypterus Walker (Hymenoptera, Eurytomidae) en la producción de semilla de Lotus corniculatus L. en diferentes fechas de floración. Montevideo, Facultad de Agronomía. Tesis Ingeniero Agrónomo. 39 p. Orientador: Alzugaray R. [Pippolo L. 1998. Incidence of the seed chalcid Bruchophagus platypterus Walker (Hymenoptera, Eurytomidae) on seed production of Lotus corniculatus L. in different flowering dates. Montevideo, Faculty of Agronomy. Bachelor Degree Thesis. 39 p. Adviser: Alzugaray R. [Spanish]] The production of seeds of Lotus corniculatus is a very important item within the Uruguayan farming system, especially in the southern-coast region. The loss of seeds caused by the "leguminous seed wasp" (Bruchophagus platypterus Walker) has been observed and mentioned in several publications but no quantification of these losses has been made. With the purpose of doing so and in an attempt to evaluate the incidence of the damage produced at different dates during the flowering stage, an experiment on a land-parcel basis was carried out during the '88-'89 crop. The experiment in question 86 Lotus literature was carried out at La Estanzuela, in Colonia. During November and December, open flowers were remarked the same day they opened and the pods were separately harvested, and manually thrashed. Furthermore, the count of -sound seeds and pods damaged by the wasps was carried out the different dates. The count of wasps and parasites was also made on each flowering date. The damage caused was expressed as the coefficient between the number of damaged seeds and the number of sound seeds, and the relative incidence of parasitoids was expressed as the coefficient between the number of parasitoids and the number of wasps. The highest damage levels were recorded during the earlier dates of flowering (39.6% and 37.7% on 9.11.88 and 16.11.88, respectively) while the lower levels were observed at the end of the period under analysis (4.46% and 5.01% on 6.12.88 and 14.12.88, respectively). In accordance with the results obtained, it was possible to corroborate the existence of Bruchophagus platypterus in Lotus corniculatus, as well as the direct damage it causes on the yield of seeds. It was furthermore proved that the number of wasps is regulated by parasitoids.
QUAGLIOTTO L., AZZIZ G., BAJSA N., VAZ P., PÉREZ C., DUCAMP F., ALTIER N. and ARIAS A. 2003. Pseudomonas strains isolated from Lotus corniculatus rhizosphere as biocontrol agents in alfalfa. Proceedings of the Sixth International PGPR Workshop. October 5-10, 2003. Calicut, India.
RISPAIL N., MORRIS P., NASH R. and WEBB K.J. 2001. Characterisation of metabolic interactions between Lotus japonicus and its symbionts (Rhizobium and arbuscular mycorrhiza). Molecular Genetics of Model Legumes: Impact for legume Biology and Breeding 15-19th Sept 2001 101.
RISPAIL N., MORRIS P., NASH R. and WEBB K.J. 2002. Influence of infection with AM fungi or Rhizobium on root flavonoid content of Lotus japonicus. Ist International Conference on Legume Genomics and Genetics. Minneapolis USA. June 2002. PM14
ROBBINS M.P., CARRON T.R. and WEBB K.J. 1993. Detecting transgenes in Lotus corniculatus using the polymerase chain reaction. LOTUS Newsletter.
ROBBINS M.P., CARRON T.R. and WEBB K.J. 1994. Detecting transgenes in Lotus corniculatus using the polymerase chain reaction. Lotus Newsletter.
ROBBINS M.P., DAVIES T.E. and WEBB K.J. 2000. Lotus japonicus as a model for studying condensed tannin pathways. Second International Conference on the Model Legume Lotus japonicus. Norwich, U.K. 24-28th June 2000. Abs. No. P41.
ROBERTS K., COUTTS J., AYRES J.F. and BILSTON L. 2002. Co-learning in the development of lotus pasture technology in Australia. Australian Journal of Experimental Agriculture, 42, 527-533. A five-year project was undertaken in New South Wales, Australia to develop and implement lotus pasture technology (Greater lotus, Lotus uliginosus; birdsfoot trefoil, Lotus corniculatus). The project was modelled on a core experiment/co-learning paradigm. A core experiment investigated issues of species adaptation and grazing management, and a co-learning phase aimed at promoting simultaneous, improvement and adoption of lotus technology. Most participants felt that involvement with co- learning gave them increased technical knowledge of lotus. Increased knowledge facilitated changes that included farming practice changes (such as establishing lotus pastures or modifications to grazing Lotus literature 87 management), or changing the approach to learning and problem solving towards a co-learning mode. The strengths of the co-learning model were that it was flexible, farmers and industry sponsors worked together, and it increased the effectiveness of the project. The weaknesses were that those groups with informal structures found it difficult to function as a team, and the time limits imposed by the project limited the learning experience.
RHODES I. and WEBB K.J. (1993) Improvement of white clover. CAB International. Outlook on Agriculture. pp. 189-194.
SACIDO M. y CAUHÉPÉ M.A. 1998. Calidad de los rebrotes posquema de pajonales de Paspalum quadrifarium. In: Berreta E. (ed.) Anales: XIV Reunión del grupo técnico regional del Cono Sur en mejoramiento y utilización de los recursos forrajeros del área tropical y subtropical: Grupo Campos. INIA Uruguay, Serie Técnica, No. 94, 73-78. ISBN 9974-38-087-1. [Sacido M. and Cauhépé M.A. 1998. Quality of the regrowth after burning grasslands with Paspalum quadrifarium. In: Berreta E. (ed.) Anales: XIV Reunión del grupo técnico regional del Cono Sur en mejoramiento y utilización de los recursos forrajeros del área tropical y subtropical: Grupo Campos. INIA Uruguay, Serie Técnica, No. 94, 73-78. ISBN 9974-38-087-1. [Spanish]]
SANDAL N., KRUSELL L., RADUTOIU S., OLBRYT M., PEDROSA A., STRACKE S., PARNISKE M., BACHMAIR A., KETELSEN T. and STOUGAARD J. 2002. An AFLP based genetic linkage map of the model legume Lotus japonicus developed from an interspecific F2 mapping population. Genetics, 161, 1673-1683.
SCHEFFER-BASSO S.M., VENDRUSCOLO M.C., BARÉA K., et al. 2002. Comportamento de leguminosas (Adesmia, Lotus, Trifolium) em mistura com festuca. R. Bras. Zootec., 31, 2197-2203. [Scheffer-Basso S.M., Vendruscolo M.C., Baréa K., et al. 2002. Response of legumes (Adesmia, Lotus, Trifolium) in mixture with tall fescue. R. Bras. Zootec., 31, 2197-2203. [Portuguese]] Overseeding temperate legumes is one of the most practices to increase the quantity, quality and sustainability of perennial pastures in Southern Brazil. During the years of 2000 and 2001 the response of six temperate legumes (Adesmia latifolia (wild), Lotus corniculatus, L. subbiflorus, L. uliginosus and Trifolium repens cv. Yi and cv. Regal) was evaluated in mixture with tall fescue (Festuca arundinacea). There was a period of 475 days between sowing date and the last cut and six cuts were made; on the autumn-winter/2000 only one cut was made, on the spring-summer, three cuts and on the autumn-winter/2001, two cuts. The intervals between cuts ranged between 43 and 91 days, being reduced in the warm season. During the total assay period, the average of the mixtures yielded 15038 kg/ha of DM; in the warm season 54% of this total was produced, with an average growth rate of 46 kg/ha/day of DM. In the cold season of 2000 and 2001 this rate was of 22 and 24 kg/ha/day of DM. The birdsfoot trefoil and white clover cv. Yi were the most productive legumes; the former was the best in the warm season (3500 kg/ha of DM) and the last participated with 86,3% in the mixture in the autumn-winter/2001, with 2300 kg/ha/day of DM. The mixtures containing these legumes presented the smallest quantity of weeds and produced 13663 and 11,184 kg/ha of DM respectively, being 82% and 71% of these totals composed of legumes, fescue and ryegrass. A. latifolia did not have a good establishment and its contribution was only 0,84% in the first cut. The L. subbiflorus had a good establishment on the first year, but it disappeared from the mixture in the second year. L. uliginosus had a good establishment and persistence producing up to 1400 kg/ha of DM in the autumn/2001.
88 Lotus literature
SCHEFFER-BASSO S.M., VOSS M. e JACQUES A.V. 2001. Nodulação e fixação biológica de nitrogênio de Adesmia latifolia e Lotus corniculatus em vasos de Leonard. Rev. Bras. Zootec., 30, 687-693. [Scheffer-Basso S.M., Jacques A.V., Voss M. 2001. Nodulation and biological Nitrogen fixation of Adesmia latifolia and Lotus corniculatus in Leonard jars. Rev. Bras. Zootec., 30, 687-693. [Portuguese]] Adesmia latifolia is a forage legume native from Southern Brazil which is outstanding due to stoloniferous growth habit and for forage production during the cool season. The Lotus corniculatus (birdsfoot trefoil) is a forage usually utilized as cool season species in subtropical and temperate regions. For both species, however, there is a reduced number of scientific works related to the nodule type and biological nitrogen fixation (BNF). This work had the purpose to analyze such processes in these species and also to compare their morphological development under different nitrogen sources: mineral-N (ammonium nitrate - 5%), symbiotic-N (inoculation) and without nitrogen (control). The experiment was carried out in greenhouse conditions, utilizing Leonard Jars with nutritive solution; the substrate consisted of mixture of sand, vermiculite and charcoal. It was a randomized complete design with four replications. At the end of 65 days the plants were harvested and evaluated for length and volume of roots, number and weight of nodules, dry matter (DM) accumulation and BNF. A. latifolia stand out for the character number of nodules (126/jar) and total nodule weight (82.22 mg DM/jar) as compared to birdsfoot trefoil with 82 nodules/jar and 20.25 mg DM/jar. The BNF was more effective in A. latifolia, whose inoculated plants produced an average of 37% of DM that was obtained by plants supplied with mineral-N, while birdsfoot reached only 15% of DM production with BNF. The amount of symbiotic fixed nitrogen was 43.12 mg N/jar in Adesmia and 9.92 mg in birdsfoot trefoil.
SCHILLER K.N. and AYRES J.F. 1993. The effects of winter conditions on the nutritive value of Lotus pedunculatus cv. Grasslands Maku and Trifolium repens cv. Haifa. Tropical Grasslands Society of Australia Newsletter, 9, 12.
SCHILLER K.N. and AYRES J.F. 1993. The effects of winter conditions on the nutritive value of Lotus pedunculatus cv. Grasslands Maku and Trifolium repens cv. Haifa. Tropical Grasslands, 27, 43-47.
SKØT L., GORDON A.J., TIMMS E., JAMES C.L., WEBB K.J. and MIZEN S. 1997. Down- regulation of sucrose synthase expression and activity in transgenic hairy roots of Lotus japonicus. Symbiosis, 22, 241-254.
SKØT L., GORDON A.J., TIMMS E., WEBB K.J. and MIZEN S. 1996. Use of antisense RNA strategies to down-regulate sucrose synthase gene expression in transgenic Lotus japonicus. Emerging Model Legume Systems: Tools and Recent Advances. Knoxville, Tennessee, USA. 13-14 July 1996. Abs. No. 23.
SKØT L., GORDON A.J., TIMMS E., WEBB K.J. and MIZEN S. 1996. Use of antisense RNA strategies to down-regulate sucrose synthase gene expression in transgenic Lotus japonicus. Molecular Plant- Microbe Interactions. Knoxville, Tennessee, USA. 14-19 July 1996. Abs. No. 28.
SKØT L., GORDON A.J., WEBB K.J., TIMMS E., JAMES C.L. and MIZEN S. 1997. Down- regulation of sucrose synthase expression and activity in transgenic hairy roots of Lotus japonicus. Society of Experimetnal Biology Meeting. 7-11 April 1997. University of Kent, Lotus literature 89
Canterbury, U.K.
SKØT L., MINCHIN F.R., TIMMS E., FORTUNE M.T., WEBB K.J. and GORDON A.J. 1995. Analysis of the two nodulins, sucrose synthase and ENOD2, in transgenic Lotus plants. The First New Phytologist Conference: Molecular Approaches to the Study of Plant-Microbe Symbioses, York November 13-17.
SKØT L., MINCHIN F.R., TIMMS E., FORTUNE M.T., WEBB K.J. and GORDON A.J. 1995. Analysis of the two nodulins, sucrose synthase and ENOD2, in root nodules of Lotus spp. using antisense strategies. Fifteenth North American Conference on Symbiotic Nitrogen Fixation; North Carolina State University, August 13-17. Abstract no 79.
SKØT L., MINCHIN F.R., TIMMS E., FORTUNE M.T., WEBB K.J. and GORDON A.J. 1996. Analysis of the two nodulins, sucrose synthase and ENOD2, in transgenic Lotus plants. Plant and Soil, 186, 99-106.
SKØT L. and WEBB K.J. 1991. Engineering an insect pest resistance gene into legume root nodules. First International Conference on Bacillus thuringiensis 28-31 July 1991. St Catherine's College, Oxford UK. Abs. No. 48.
SKØT L. and WEBB K.J. 1991. Engineering an insect pest resistance gene into legume root nodules. 3rd AFRC Meeting on Plant and Soil Nitrogen Metabolism. UCW Aberystwyth. Abs. No. 19.
SKØT L. and WEBB K.J. 1991. Engineering an insect resistance gene into Rhizobium and legumes. 4th International Society of Plant Molecular Biology, Tucson, University of Arizona, USA. 6-12 October 1991. Abs. No. 1406.
SKØT L. and WEBB K.J. 1991. Engineering insect pest resistance into Rhizobium and legumes. Symbiosis and Crop Growth. The Rank-Prize Funds. Mini-Symposium on Symbiosis and Crop Growth. Molecular and Organismal Aspects. Organisers: The Nutrition Committee. Grasmere, Cumberland, UK. 30th April - 3rd May 1991.
SKØT L., WEBB K.J., NICHOLSON M.M., JØRGENSEN B. and MIZEN S. 1998. Use of T-DNA tagging to identify plant genes involved in symbiotic nitrogen fixation. 16th North American Conference on Symbiotic Nitrogen Fixation. Feb 1-6, 1998. Cancun, Mexico.
SKØT L., WEBB K.J., NICHOLSON M.M., JØRGENSEN B. and MIZEN S. 1999. Use of T-DNA tagging to identify plant genes involved in symbiotic nitrogen fixation. In: Martínez and Hernández. (Eds) Highlights of Nitrogen Fixation Research. 16th North American Conference on Symbiotic Nitrogen Fixation. 1- 6 Feb, 1998. Cancun, Mexico. 153- 156. Plenum Publishing Corporation, New York.
SOKOLOFF D.D. 1998. What is Tetragonolobus wiedemannii Boiss. (Fabaceae)? Novit. Syst. Pl. Vasc. (St. Petersburg), 31, 139-142. [Russian]. Tetragonolobus wiedemannii = Lotus wiedemannii is in fact a synonym of Hammatolobium 90 Lotus literature lotoides.
SOKOLOFF D.D. 1999. Typification of several specific and generic names in the tribe Loteae (Papilionaceae). Taxon., 48, 57-59. Several species of Lotus are typified. The species Lotus wrangelianus is moved into the genus Acmispon. The genus Syrmatium (a genus segregated from Lotus) is typified.
SOKOLOFF D.D. 1999. Ottleya, a new genus of Papilionaceae-Loteae from North America. Feddes Repert., 110, 89-97. The genus is segregated from Lotus and corresponds to Lotus sect. Simpeteria Ottley.
SOKOLOFF D.D. 2000. New combinations in Acmispon (Leguminosae, Loteae). Ann. Bot. Fennici., 37, 125-131. Acmispon is an American genus segregated from Lotus.
SOKOLOFF D.D. 2000. New combinations in Hosackia Douglas ex Benth. (Leguminosae: Loteae). Kew Bull., 55, 1009-1010. Hosackia is a North American genus segregated from Lotus.
SOKOLOFF D.D. 2001. New records of Lotus (Leguminosae: Papilionoideae: Loteae) from Africa and southwest Asia. Kew Bull., 56, 715-720.
SOKOLOFF D.D. 2003. On system and phylogeny of the tribe Loteae DC. (Leguminosae). Bull. Moscow Soc. Natur. Biol. Ser., 108, 35-48. [Russian]. The following new combinations and names in Lotus and its segregate genera are published: Hosackia sect. Protohosackia D.D. Sokoloff, Ottleya plebeia (Brandegee) D.D. Sokoloff, Ottleya mollis (A.A. Heller) D.D. Sokoloff et K.N. Gandhi, Lotus sect. Bonjeanea (Reichenb.) D.D. Sokoloff, Lotus sect. Dorycnium (Mill.) D.D. Sokoloff, Lotus axilliflorus (Hub.-Mor.) D.D. Sokoloff, Lotus fulgurans (Porta) D.D. Sokoloff, Lotus sanguineus (Vural) D.D. Sokoloff, Lotus sect. Canaria (Rikli) D.D. Sokoloff, Lotus sect. Rhyncholotus (Monod) D.D. Sokoloff.
Lotus literature 91
SOSTER M.T.B., SCHEFFER-BASSO S.M. e DALL’AGNOL M. 2003. Caracterização morfofisiológica de genótipos de cornichão (Lotus corniculatus L.). Submetido à Revista SBZ. [Soster M.T.B., Scheffer-Basso S.M. and Dall’agnol M. 2003. Morphophysiological characterization on the birdsfoot trefoil (Lotus corniculatus L.). Submeted to Revista SBZ. [Portuguese]] The birdsfoot trefoil cv. São Gabriel is an important winter-spring growing legume in the Southern Brazil, which main limitation is the low persistence under grazing. The objective of this work was to evaluate eight populations of this cultivar, selected under grazing or cut, comparing with two rhizomatous genotypes and a cultivar from Uruguay (Agrosan Trueno), with the purpose to evaluate the morphological variability and characters linked to grazing tolerance. Two assays were conducted in the greenhouse with plants grown in pots until the full flowering stage. It was observed morphological variability in the populations, with variation in the leaf, stem and crown morphology as well as in growth habit. Only in the rhizomatous genotypes were observed tipical rhizomes, but all the genotypes showed subterraneous stems. The rhizomatous genotypes were susceptible to mites and two populations showed symptoms of anthracnose. There are some morphophysiological variability of the cv. São Gabriel populations can be used in a birdsfoot trefoil breeding programs.
SOSTER M.T.B., SCHEFFER-BASSO S.M., DALL’AGNOL M., BRUSTOLIN R.E and FONTANELI R.S. 2003. Caracterização agronômica de genótipos de cornichão (Lotus corniculatus L.). Submetido à Revista SBZ. [Soster M.T.B., Scheffer-Basso S.M., Dall’agnol M., Brustolin R.E and Fontaneli R.S. 2003. Agronomic characterization if birdsfoot trefoil genotypes (Lotus corniculatus L.) This work was carried out to characterize agronomically birdsfoot trefoil (Lotus corniculatus L.) genotypes, including eight populations derived from the cultivar São Gabriel, one rhizomatous cultivar (ARS-2620) and one cultivar without rhizomes (Trueno). Individual plants were submitted to seven cuts under field conditions during one year, at Passo Fundo, Rio Grande do Sul, Brazil. There was little variation among the populations in relation to forage yield, quality and persistence. On the average, the populations and the cultivar Trueno were about 50% more productive than the cultivar ARS-2620. The persistence presented a range varying from 80% to 100% of survival. The crude protein content decreased from 21,8 (vegetative) to 11,2% (flowering). The overall mean for fiber insoluble in acid detergent was 24,2% and the fiber insoluble in neutral detergent ranged from 55,3% to 58,8%. Overall, the populations presented a higher yield potential in relation to the cultivar ARS-2620.
STENGLEIN S.A., COLARES M.N., ARAMBARRI A.M., NOVOA M.C., VIZCAÍNO C.E. and KATINAS L. 2003. Leaf epidermal microcharacters of the Old World species of Lotus (Leguminosae: Loteae) and their systematic significance. Australian Journal of Botany, 51, 1-11.
STENGLEIN S.A., ARAMBARRI A.M., COLARES M.N., NOVOA M.C. and VIZCAÍNO C.E. 2003. Epidermal characteristics of the New World species of Lotus: subgenus Acmispon (Fabaceae: Loteae) and a numerical taxonomic evaluation. Canadian Journal of Botany, 81, 933-944.
TIKHOMIROV V.N. and SOKOLOFF D.D. 1997. Taxonomic position of Vermifrux abyssinica (A. Rich.) Gillett and taxonomy of the tribe Loteae s.l. (Papilionaceae). Feddes Repert., 108, 335-344. The name Vermifrux was included by Polhill (1981) into synonymy of Lotus. We have 92 Lotus literature demonstrated that this genus should be merged with Dorycnopsis.
TSUTSUPA T.A., BARYKINA R.P., KRAMINA T.E. and SOKOLOFF D.D. 2001. On the reduction of terminal bud in seedlings of some papilionoid legumes // Feddes Repert., 112, 459-467. Morphological and anatomical structure of seeds and seedlings of Securigera securidaca (L.) Degen & Doerfl. and several other members of Loteae tribe [Ornithopus sativus Brot., Lotus tetragonolobus L., Tripodion tetraphyllus (L.) Fourr. and Hymenocarpus circinnatus (L.) Savi] was studied. Development of the plumule, cotyledons, shoots system and main root of the seedlings are described with special attention to the vascular system. In cotyledonary axils, serial buds early appear and develop that makes identification of the main shoot rather difficult. However, the apical bud and normally developed main shoot were found in all examined species. Dormer’s (1945) idea on the reduction of the main bud and Compton’s (1912) supposition on extra-axillary branching of epicotyl into equivalent shoots were disproved.
TUCK E., COOK R., SKØT L., WEBB K.J., COOMBER S. and PARNISKE M. 2001. Signal transduction during early symbiotic events (2001). Molecular Genetics of Model Legumes: Impact for legume Biology and Breeding 15-19th Sept 2001 109.
TUCK E., COOK R., SKØT L., WEBB K.J., COOMBER S. and PARNISKE M. 2002. Symbiosis- specific expression of a gene encoding a calcium-binding protein in the model legume Lotus japonicus during early interactions with root-nodulating bacteria (Mesorhizobium loti) and the arbuscular mycorrhiza fungi Glomus mosseae. Plant Science Wales 7-8th January, Bangor. Abs no. 4.
TUCK, E., COOK R., SKØT L., WEBB K.J., COOMBER S. and PARNISKE M. 2002. Expression of a gene encoding a calcium-binding protein in the model legume Lotus japonicus during early interactions with root-nodulating bacteria (Mesorhizobium loti) and the arbuscular mycorrhiza fungi Glomus mosseae. Society of Experimental Biology April 2002 Swansea. Abs no. P8.2.
TUCK E., COOK R., SKØT L., WEBB K.J., COOMBER S. and PARNISKE M. 2002. Identiying symbiosis-specifc genes in the model legume Lotus japonicus. Ist International Conference on Legume Genomics and Genetics. Minneapolis USA. June 2002. PM17
TUCK E., MUR L., SKØT L., COOK R., PARNISKE M. and WEBB K.J. 2000. Early gene expression during plant-microbe interactions. Second International Conference on the Model Legume Lotus japonicus. Norwich, U.K. 24-28th June 2000. Abs. No. P48.
WANG T.L., DOMONEY C., HEDLEY C.L., CASEY R. and GRUSAK M.A. 2003. Can we improve the nutritional quality of legume seeds? Plant Physiology, 131, 886-891.
WEBB K.J. 1985. Transformation of the forage legumes using the natural gene vector, Agrobacterium tumefaciens. White Clover Discussion Group meeting, AGRI Hurley 1- 2 August 1985.
Lotus literature 93
WEBB K.J. 1986. Transformation in the forage legumes. Genetic Manipulation in Plant Breeding. Eds: W Horn, CJ Jensen, W Odenbach and O Schieder. Proc. International Symposium organised by Eucarpia. 8-13 Sept 1985. Berlin (West) Germany. 831-833.
WEBB K.J. 1986. Transformation of forage legumes using Agrobacterium tumefaciens. Theoretical Applied Genetics, 72, 53-58.
WEBB K.J. 1988. Recent developments in the regeneration of agronomically important crops from protoplasts. Plant Cell, Tissue and Organ Culture, 12, 127-131.
WEBB K.J. 1990. Transformation in herbage legumes. UCW Cell Genetics Group Meeting UK 8th-9th January 1990.
WEBB K.J. 1996. Opportunities for Biotechnology in Forage Legume Breeding. Legumes in Sustainable Farming Systems. British Grassland Occasional Symposium No. 30. 2-4 September Aberdeen, Scotland. Ed D Younie. pp. 77-85.
WEBB K.J. and ARMSTEAD I.P. 1986. Transformation in the herbage legumes. IAPTC, UK Symposium, Edinburgh, UK. 1986.
WEBB K.J., CHAMBERLAIN D.A., WOODCOCK S. and DALE P.J. 1986. Cell and protoplast culture in forage legumes. In: Genetic Engineering of Plants and Micro-organisms 88-89.
WEBB K.J., EASON W., ABBERTON M., MICHAELSON-YEATES T., TEODSIO H.R., PARNISKE M. and HOOKER J. 2000. Tools for unravelling genetic control of arbuscular mycorrhizal symbiosis in legumes.
WEBB K.J., FAY M.F., WOODCOCK S., PIKE L.S. and DALE P.J. 1985. Protoplast culture of forage legumes. In: Genetic Engineering of Plants and Micro-organisms Important for Agriculture. Eds: E Magnien & D de Nettancourt. Martinus Nijhoff 162-163.
WEBB K.J., FAY M.F., PIKE L.S., WOODCOCK S. and DALE P.J. 1984. Selection of responsive genotypes for protoplast culture in forage legumes. British Grassland Society Occasional Symposium No 16. In: Forage Legumes. Ed: DJ Thomson 168 -169.
WEBB K.J., FAY M.F., JONES M.G.K., PIKE L.S., WOODCOCK S. and DALE P.J. 1983. Protoplast culture in forage legumes. Research and training programme in Biomolecular Engineering, Louvain-la-Neuve, Belgium. Abstract no 83.
WEBB K.J., FAY M.F., JONES M.G.K., PIKE L.S., WOODCOCK S. and DALE P.J. 1983. Protoplast culture in forage legumes. AFRC Genetic Manipulation of Crop Plants: Five years on 12-13 December , University of Cambridge, UK. Abstract no 76.
WEBB K.J., GIBBS M., MIZEN S., SKØT L. and GATEHOUSE J. 1996. Genetic transformation of Lotus corniculatus with Agrobacterium tumefaciens and the analysis of the inheritance of trangenes in the T1 generation. Transgenic Research, 5, 303-312. 94 Lotus literature
WEBB K.J., HUMPHREYS M.O., SKØT L., GIBBS M. and GATEHOUSE J. 1999. Inheritance and expression of transgenes in T2 and T3 generations of Lotus corniculatus transformed using Agrobacterium tumefaciens. Euphytica, 108, 169-179.
WEBB K.J., JONES S. and GIBBS M. 1990. Use of Agrobacterium rhizogenes binary vector to transform herbage legumes. VIIth International Congress on Plant Tissue and Cell Culture, Amsterdam 24-29 June 1990. Abstr. no.A2-147.
WEBB K.J., JONES S., ROBBINS M.P. and MINCHIN F.R. 1990. Characterization of transgenic root cultures of Trifolium repens, T.pratense and Lotus corniculatus and transgenic plants of L.corniculatus. Plant Science, 70, 243-254.
WEBB K.J., JØRGENSEN B., SKØT L. and DOWNIE J.A. 1994. Evaluation of Lotus japonicus for a T-DNA tagging programme. PMBII Mid-term Meeting, University of East Anglia, 6-8th July, Norwich.
WEBB K.J., MEREDITH M.R. and NISBET G.S. 1986. Study of variation in regenerants of Lotus corniculatus. IAPTC, UK Symposium, Edinburgh, UK. 1986.
WEBB K.J., MIZEN S. and COOKE D.E. 1994. A long term study of GUS activity in hairy root cultures and primary transformants in Lotus corniculatus. LOTUS Newsletter. Ed: PR Beuselinck USDA-Agricultural Research Service. 25:28-30.
WEBB K.J., MIZEN S., ROBBINS M.P., GATEHOUSE J.A. and ROWE M.J. 1994. Patterns of inheritance of transgenes in the herbage legumes Lotus corniculatus transformed by Agrobacterium rhizogenes and A.tumefaciens. VIII International Congress on Plant Tissue and Cell Culture. Firenze, 12-17 June 1994.
WEBB K.J. and MORRIS P. 1992. Methodologies of Plant Transformation. In: Gatehouse A., Hilder V.A. and Boulter D. (Eds) Plant Genetic Engineering for Crop Protection. CAB International. pp. 7-43.
WEBB K.J., NICHOLSON M.M., JØRGENSEN B., MIZEN S. and SKØT L. 1996. T-DNA mutagenesis in Lotus japonicus. Emerging Model Legume Systems: Tools and Recent Advances. Knoxville, Tennessee July 12-14, 1996. Abs. No. 5.
WEBB K.J. and RHODES I. 1991. Opportunities for biotechnology in clover breeding. Fodder Crops Breeding: Achievements Novel Strategies and Biotechnology. Proceedings of the 16th Meeting of Eucarpia, Wageningen, The Netherlands. Eds: APM den Dijs and A Elgersman. Pudac, Wageningen. 18-22 November 1990. pp. 111-116.
WEBB K.J., ROBBINS M.P. and MIZEN S. 1993. Expression of GUS in primary transformants and the inheritance of GUS, TL and TR in progeny of Lotus corniculatus L. International Symposium on Genetic Manipulation of Plant Metabolism and Growth. University of East Anglia, Norwich, UK. 29-31 March 1993. Abstract no 1. Lotus literature 95
WEBB K.J., ROBBINS M.P. and MIZEN S. 1994. Expression of GUS in primary transformants and segregation patterns of GUS, TL- and TR-DNA in the T1 generation of hairy root transformants of Lotus corniculatus. Transgenic Research, 3, 232-240.
WEBB K.J., ROBBINS M.P. and MIZEN S. 1994. Segregation of Agrobacterium rhizogenes T- DNA from other inserted genes in the T1 progeny of Lotus corniculatus. Proceedings First International Lotus Symposium, 22-24 March 1994, St Louis, Missouri, USA. Ed: P Beuselinck.
WEBB K.J., ROBBINS M.P. and MIZEN S. 1994. 1st International Symposium, St Louis, USA. February 1994.
WEBB K.J. and SKØT L.. 2000. A calcium binding protein homologue identified using promoter tagging in Lotus japonicus. Second International Conference on the Model Legume Lotus japonicus. Norwich, U.K. 24-28th June 2000. Abs. No.S51.
WEBB K.J., SKØT L., COOKE D.E., GIBBS M.J., MIZEN S. and ROBBINS M.P. 1995. Expression and inheritance of transgenes in Lotus corniculatus. XIV EUCARPIA. Adaptation in Plant Breeding. Jyväskylä, Finland. July 31-Aug 4. p121.
WEBB K.J., SKØT L. and DOWNIE A. 1994. Identification of plant genes specifically involved in nodulation and nitrogen fixation. PMBII Mid-Term Meeting 6-8th July 1994.
WEBB K.J., SKØT L. and DOWNIE J.A. 1994. Identification of plant genes specifically involved in nodulation and nitrogen fixation. PMBII Mid-term Meeting, University of East Anglia, 6- 8th July, Norwich.
WEBB K.J., SKØT L. and JØRGENSEN B. 1994. Plant genes involved in nodule development and nitrogen fixation. Proceedings of the 1st European Nitrogen Fixation Conference, Szeged, Hungary. August 29 - September 2. Eds: GB Kiss and G Endre. Officina Press. pp. 234-238.
WEBB K.J., SKØT L., and JØRGENSEN B. 1995. Progress in mutagenesis, mapping and carbon metabolism in Lotus japonicus. EU Project of Technological Priority. Joint Meeting of Themes D1 and D2. 2nd Joint Meeting on Nitrogen Utilization Efficiency. September 1995, Sevilla, Spain.
WEBB K.J., SKØT L., MIZEN S., PARNISKE M., JØRGENSEN B. and NICHOLSON M. 1998. Identification of genes expressed in roots and nodules of Lotus japonicus using a promoter trap. Plant Biotechnology and In vitro Biology in the 21st Century. IX International Congress on Plant Tissue and Cell Culture, Jerusalem, Israel, 14-19 June 1998. p. 44.
WEBB K.J., SKØT L., MIZEN S., PARNISKE M., JØRGENSEN B. and NICHOLSON M. 1998. Using EMS mutagenesis and promoter trapping to search for mutants and promoters in symbiotic interactions of Lotus japonicus. First International Lotus japonicus Workshop. 96 Lotus literature
20-22nd November 1998, University of Aarhus, Denmark.
WEBB K.J., SKØT L., MIZEN S., PARNISKE M., JØRGENSEN B. and NICHOLSON M. 1999. Identification of genes expressed in roots and nodules of Lotus japonicus using a promoter trap. In: Altman A., Ziv M. and Izhar S. (Eds) Current Plant Science and Biotechnology in Agriculture. Plant Biotechnology and In vitro Biology in the 21st Century. IX International Congress on Plant Tissue and Cell Culture, Jerusalem, Israel, 14-19 June 1998. 227-229.
WEBB K.J., SKØT L., NICHOLSON M.N., JØRGENSEN B. and MIZEN S. 2000. Mesorhizobium loti increases ot-specific expression of a calcium-binding protein homologue identified by promoter tagging in Lotus japonicus. Molecular Plant-Microbe Interactions, 13 (6), 606- 616.
WEBB K.J. and WATSON E.J. 1991. Lotus corniculatus L.: Morphological and cytological analysis of regenerants from three sources of tissue and selected progeny. Plant, Cell, Tissue and Organ Culture, 25, 27-33.
WEBB K.J., WOODCOCK S. and CHAMBERLAIN D.A. 1987. Plant regeneration from protoplasts of Trifolium repens and Lotus corniculatus. Journal of Plant Breeding, 98, 111-118.
WEBB K.J., WOODCOCK S., PIKE L.S. and DALE P.J. 1986. Plant regeneration in the forage legumes. In: Plant Tissue Culture and its Agricultural Applications. Eds: LA Withers and PG Alderson. 17-21 Sept 1984. Butterworth Scientific, London. 99-104.
WEBB K.J., WOODCOCK S., PIKE L.S., FAY M.F. and DALE P.J. 1984. Protoplast culture in forage legumes. University of Wales Staff Colloquium. Biotechnology 20-21 March.
WILSON G.R.M. and ORAM R.N. 1991. B. Legumes 22. Lotus (a) Lotus pedunculatus Cav. (greater lotus) cv. Sharnae. Register of Australian herbage plant cultivars, pp 794-795.
WOOD F.J. 1997. Comparison of the growth periodicity and population dynamics of Lotus pedunculatus (cv Grasslands Maku) and Lotus corniculatus (cv Grasslands Goldie) in a summer rainfall environment. Honours dissertation, Department of Crop Sciences, The University of Sydney. Dr. John Ayres
YOUNG S.R. [in association with HUMPHREYS M., ABBERTON M., ROBBINS M. and WEBB K.J.] 1999. The risks associated with the introduction of GM forage grasses and forage legumes. Report to MAFF.