Epigen, human Recombinant Human Epigen

Instruction Manual

Catalog Number C-68380 Synonyms EPG, Epigen, PRO9904, ALGV3072, FLJ75542, EPGN, Epithelial Description Epigen is an EGF-related polypeptide that signals through the ErbB receptor-1. It is produced in several tissues, including the testis, liver, heart and in certain tumor cells. Epigen is mitogenic for fibroblasts and epithelial cells. Human Epigen is initially synthesized as a glycosylated 14.7 kDa transmembrane precursor , which is processed by proteolytic cleavage to produce a mature soluble sequence. Recombinant human Epigen is a 7.9 kDa non-glycosylated, monomeric protein consisting of 72 amino acid residues, which comprises the EGF homologous portion of the Epigen precursor. The rHu Epigen has been purified by proprietary chromatographic techniques. Quantity 25 µg Molecular Mass 7.9 kDa Source E. coli Biological-Activity Typically determined by the dose-dependent stimulation of the proliferation of murine Balb/3T3 cells. Specific Activity NA Formulation White lyophilized (freeze-dried) powder. Lyophilized from a sterile-filtered protein solution in 20 mM PBS buffer (pH 7.4) and 130 mM sodium chloride. Reconstitution Please Note: Always centrifuge product before opening vial. The lyophilized protein should be reconstituted with sterile, ultrapure water to a concentration of 0.1 - 1.0 mg/ml. For further dilution, carrier protein (0.1% BSA or HSA) should be added to avoid loss of bioactivity. Purity Greater than 98% (determined by SDS-PAGE and RP-HPLC analysis) Endotoxin Level < 0.1 ng per µg of rHu Epigen (1EU/µg). Storage The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% endotoxin-free HSA or BSA; e.g. Cat.No. C-69500A). Avoid repeated freeze-thaw cycles.

05/2019