MONOCLONAL 1B4C3

Antibody name: 1B4C3 : HER3, erbB3 : IgG2a Immunogen: fusion protein of extracellular domain of HER3 and a C- terminal His-Tag (HER3-6x His-CT) Reactivity: reast tumor cells Reference: van der Horst EH, Murgia M, Treder M, Ullrich A. Anti-HER- 3 MAbs inhibit HER-3-mediated signaling in breast cancer cell lines resistant to anti-HER-2 . Int J Cancer. 2005;115:519-27.

MONOCLONAL ANTIBODY 1C1

Antibody name: 1C1 Antigen: human CD117 (c-kit) Isotype: IgG1 Immunogen: MOLM-1 megakaryocitic cell line Reactivity: Hematopoietic stem cells, mast cells Reference: HLDA-6, Myeloid Workshop (Kobe, Japan) 1D4F2

Antibody name: 1D4F2 Antigen: CD203c (ecto-nucleotide pyrophosphatase/phosphodiesterase 3, E-NPP3) Immunogen: soluble E-NPP3 Isotype: IgG1 Reactivity: and mast cells. activation marker. Secretory glands in endometrium. Reference: Bühring HJ, Simmons PJ, Pudney M, Müller M, Jarrossay D, van Agthoven A, Willheim M, Brugger W, Valent P & Kanz L: The monoclonal antibody 97A6 defines a novel surface antigen expressed on human basophils and their multi- and unipotent progenitors. (1999) Blood 94:2343- 2356.

Bühring HJ, Seiffert M, Giesert C, Marxer A, Valent P, Kanz L & Sano K: The basophil activation marker defined by antibody 97A6 is identical with the ecto-nucleotide pyrophosphatase/phosphodiesterase 3 (E-NPP3; CD203c). (2001) Blood 97:3303-3305.

Platz IJ, Binder M, Marxer A, Lischka G, Valent P & Bühring HJ: Hymenoptera venom-induced upregulation of the basophil activation marker ecto-nucleotide pyrophosphatase/phosphodiesterase 3 (E-NPP3, CD203c) in sensitized individuals. (2001) Int Arch Immunol 126:335-342.

HLDA8 (Adelaide, 2008)

MONOCLONAL ANTIBODY 1G2

Antibody name: 1G2 Antigen: human CD105 (endoglin) Isotype: IgG3 Immunogen: Leukemic blasts Reactivity: L-cells transfected with human CD105, immature erythroid cells, endothelial cells (all stages), mesenchymal cells. Reference: 1. http://mpr.nci.nih.gov/prow/guide/948384634_g.htm 2. Bühring HJ, Müller C, Letarte M, Gougos A, Saalmüller A, Van Agthoven A, Busch FW. Endoglin is expressed on a subpopulation of immature erythroid cells of normal human bone marrow. Leukemia. 1991;5:841-847.

MONOCLONAL ANTIBODY 2D1D12

Antibody name: 2D1D12 Antigen: HER3, erbB3 Isotype: IgG1 Immunogen: fusion protein of extracellular domain of HER3 and a C- terminal His-Tag (HER3-6x His-CT) Reactivity: Breast tumor cells Reference: van der Horst EH, Murgia M, Treder M, Ullrich A. Anti-HER- 3 MAbs inhibit HER-3-mediated signaling in breast cancer cell lines resistant to anti-HER-2 antibodies. Int J Cancer. 2005;115:519-27.

MONOCLONAL ANTIBODY 2E1A6

Antibody name: 2E1A6 Antigen: not identified Isotype: IgG1 Immunogen: HEK-293 embryonic kidney cells Reactivity: Subsets of bone marrow CD34+ cells. Reference: no reference MONOCLONAL ANTIBODY 2E4B4

Antibody name: 2E4B4 Antigen: not identified Isotype: IgG2a Immunogen: HEK-293 embryonic kidney cells Reactivity: Subsets of bone marrow CD34+ cells. Reference: no reference MONOCLONAL ANTIBODY 3A2C2

Antibody name: 3A2C2 Antigen: CD203c (ecto-nucleotide pyrophosphatase/phosphodiesterase 3, E-NPP3) Immunogen: soluble E-NPP3 Isotype: IgG1 Reactivity: Basophils and mast cells. Basophil activation marker. Secretory glands in endometrium. Reference: Bühring HJ, Simmons PJ, Pudney M, Müller M, Jarrossay D, van gthoven A, Willheim M, Brugger W, Valent P & Kanz L: The monoclonal antibody 97A6 defines a novel surface antigen expressed on human basophils and their multi- and unipotent progenitors. (1999) Blood 94:2343-2356.

Bühring HJ, Seiffert M, Giesert C, Marxer A, Valent P, Kanz L & Sano K: The basophil activation marker defined by antibody 97A6 is identical with the ecto-nucleotide pyrophosphatase/phosphodiesterase 3 (E-NPP3; CD203c). (2001) Blood 97:3303-3305.

Platz IJ, Binder M, Marxer A, Lischka G, Valent P & Bühring HJ: Hymenoptera venom-induced upregulation of the basophil activation marker ecto-nucleotide pyrophosphatase/phosphodiesterase 3 (E-NPP3, CD203c) in sensitized individuals. (2001) Int Arch Allergy Immunol 126:335-342.

HLDA8 (Adelaide, 2008)

MONOCLONAL ANTIBODY 3C2C1

Antibody name: 3C2C1 Antigen: CD203c (ecto-nucleotide pyrophosphatase/phosphodiesterase 3, E-NPP3) Immunogen: soluble E-NPP3 Isotype: IgG2a Reactivity: Basophils and mast cells. Basophil activation marker. Secretory glands in endometrium. Reference: Bühring HJ, Simmons PJ, Pudney M, Müller M, Jarrossay D, van gthoven A, Willheim M, Brugger W, Valent P & Kanz L: The monoclonal antibody 97A6 defines a novel surface antigen expressed on human basophils and their multi- and unipotent progenitors. (1999) Blood 94:2343-2356.

Bühring HJ, Seiffert M, Giesert C, Marxer A, Valent P, Kanz L & Sano K: The basophil activation marker defined by antibody 97A6 is identical with the ecto-nucleotide pyrophosphatase/phosphodiesterase 3 (E-NPP3; CD203c). (2001) Blood 97:3303-3305.

Platz IJ, Binder M, Marxer A, Lischka G, Valent P & Bühring HJ: Hymenoptera venom-induced upregulation of the basophil activation marker ecto-nucleotide pyrophosphatase/phosphodiesterase 3 (E-NPP3, CD203c) in sensitized individuals. (2001) Int Arch Allergy Immunol 126:335-342.

HLDA8 (Adelaide, 2008)

MONOCLONAL ANTIBODY 4C1B12 (also 4C1D12)

Antibody name: 4C1B12 (also 4C1D12) Antigen: CD203c (ecto-nucleotide pyrophosphatase/phosphodiesterase 3, E-NPP3) Immunogen: soluble E-NPP3 Isotype: IgG2b Reactivity: Basophils and mast cells. Basophil activation marker. Secretory glands in endometrium. Reference: Bühring HJ, Simmons PJ, Pudney M, Müller M, Jarrossay D, van gthoven A, Willheim M, Brugger W, Valent P & Kanz L: The monoclonal antibody 97A6 defines a novel surface antigen expressed on human basophils and their multi- and unipotent progenitors. (1999) Blood 94:2343-2356.

Bühring HJ, Seiffert M, Giesert C, Marxer A, Valent P, Kanz L & Sano K: The basophil activation marker defined by antibody 97A6 is identical with the ecto-nucleotide pyrophosphatase/phosphodiesterase 3 (E-NPP3; CD203c). (2001) Blood 97:3303-3305.

Platz IJ, Binder M, Marxer A, Lischka G, Valent P & Bühring HJ: Hymenoptera venom-induced upregulation of the basophil activation marker ecto-nucleotide pyrophosphatase/phosphodiesterase 3 (E-NPP3, CD203c) in sensitized individuals. (2001) Int Arch Allergy Immunol 126:335-342.

HLDA8 (Adelaide, 2008)

MONOCLONAL ANTIBODY 4FR6D3

Antibody name: 4FR6D3 Antigen: human CD334 (FGFR4) Isotype: IgG1 Immunogen: NIH-3T3/huFGFR4 Reactivity: Subset of breast tumor cells Reference: Leucocyte Typing VIII; http://www.hcdm.org MONOCLONAL ANTIBODY 4G8

Antibody name: 4G8 Antigen: human CD135 (FLT3) Isotype: IgG1 Immunogen: BaF3/hu-FLT3 cell line Reactivity: Subset of stem cells, subsets of leukemic blasts. Reference: HLDA6 (Kobe): Bühring HJ, Birnbaum D, Brasel K, Civin CI, Götze K, Lyman S, Rappold I, Rosnet O. CD135 workshop panel report. In: Leucocyte Typing VI. White Cell Differentiation . Kishimoto T, Kikutani H, von dem Borne AEGKr, Goyert SM, Mason DY, Miyasaka M, Moretta L, Okumura K, Shaw S, Springer TA, Sugamura K & Zola H (eds), Garland Publishing, Inc, New York. 1998; pp 875-79. Rappold I, Ziegler BL, Köhler I, Marchetto, S, Rosnet O, Birnbaum D, Simmons PJ, Zannettino ACW, Hill B, Neu S, Knapp W, Alitalo R, Alitalo K, Ullrich A, Kanz L, Bühring HJ. Functional and phenotypic characterization of cord blood and bone marrow subsets expressing FLT3 receptor tyrosine kinase. Blood. 1997;90:111-125.

MONOCLONAL ANTIBODY 7C5G1

Antibody name: 7C5G1 Antigen: not identified Isotype: IgG1 Immunogen: DU4475 breast carcinoma cells Reactivity: Primary bone marrow MSC Reference: Bühring HJ, Battula VL, Treml S, Schewe B, Kanz L, Vogel W. Novel markers for the prospective isolation of human MSC. Ann New York Acad Sci 2007; 1106:262-71. Bühring HJ, Treml S, Cerabona F, de Zwart P, Kanz L, Sobiesiak M. Phenotyopic characterization of distinct human bone marrow- derived MSC subsets. Hematopoietic Stem Cells VII: Ann NY Sci 2009;1176:124-134.

MONOCLONAL ANTIBODY 9A3G9

Antibody name: 9A3G9 Antigen: not identified Isotype: IgG1 Immunogen: DU4475 breast carcinoma cells Reactivity: Primary bone marrow MSC Reference: Bühring HJ, Battula VL, Treml S, Schewe B, Kanz L, Vogel W. Novel markers for the prospective isolation of human MSC. Ann New York Acad Sci 2007; 1106:262-71. Bühring HJ, Treml S, Cerabona F, de Zwart P, Kanz L, Sobiesiak M. Phenotyopic characterization of distinct human bone marrow- derived MSC subsets. Hematopoietic Stem Cells VII: Ann NY Sci 2009;1176:124-134.

MONOCLONAL ANTIBODY 9C4

Antibody name: 9C4 Antigen: CD326 (EpCAM) Isotype: IgG2b Immunogen: DU4475 breast carcinoma cells Reactivity: Tumor cells, erythroblasts Reference: Lammers R, Giesert C, Grünebach F, Marxer A, Vogel W, Bühring HJ. Monoclonal antibody 9C4 recognizes epithelial cellular adhesion molecule, a cell surface antigen expressed in early steps of erythropoiesis. Exp Hematol. 2002;30:537-545.

MONOCLONAL ANTIBODY 11H6

Antibody name: 11H6 Antigen: CD18 (beta2 integrin) Isotype: IgG2a Immunogen: Leukemic blasts Reactivity: Broad range of reactivity. Most leukocytes are positive Reference: HLDA-4, Myeloid Workshop (Vienna, Austria) MONOCLONAL ANTIBODY 13A1

Antibody name: 13A1 Antigen: CD340 (HER2, erbB2) Isotype: IgG2a Immunogen: NIH-3T3/huHER-2 cells Reactivity: Tumor cells, subset of ALL blasts, bone marrow MSC Reference: http://www.hcdm.org Bühring HJ, Sures I, Jallal B, Weiß U, Busch FW, Ludwig WD, Handgretinger R, Waller HD, Ullrich A. The receptor tyrosine kinase p185HER2 is expressed on a subset of B-lymphoid blasts from patients with acute lymphoblastic leukemia and chronic myelogenous leukemia. Blood. 1995;86:1916-1923.

MONOCLONAL ANTIBODY 13D1

Antibody name: 13D1 Antigen: CD340 (HER2, erbB2) Isotype: IgG2a Immunogen: NIH-3T3/huHER-2 cells Reactivity: Tumor cells, subset of ALL blasts, bone marrow MSC Reference: http://www.hcdm.org Bühring HJ, Sures I, Jallal B, Weiß U, Busch FW, Ludwig WD, Handgretinger R, Waller HD, Ullrich A. The receptor tyrosine kinase p185HER2 is expressed on a subset of B-lymphoid blasts from patients with acute lymphoblastic leukemia and chronic myelogenous leukemia. Blood. 1995;86:1916-1923.

MONOCLONAL ANTIBODY 16A1

Antibody name: 16A1 Antigen: CD140a (PDGF-RA) Isotype: IgG1 Immunogen: NIH-3T3/huPDGFRA cells Reactivity: bone marrow MSC Reference: Miyazaki S, et al.. Cross-lineage (Blind Panel) study and human leucocyte differentiation antigen database. In: Leucocyte Typing VI. White Cell Differentiation Antigens. Kishimoto T, Kikutani H, von dem Borne AEGKr, Goyert SM, Mason DY, Miyasaka M, Moretta L, Okumura K, Shaw S, Springer TA, Sugamura K & Zola H (eds), Garland Publishing, Inc, New York. 1998; pp 3-20. MONOCLONAL ANTIBODY 18A2

Antibody name: 18A2 Antigen: CD140b (PDGF-RB) Isotype: IgG1 Immunogen: NIH-3T3/huPDGFRB cells Reactivity: bone marrow MSC Reference: Leucocyte Typing VIII; http://www.hcdm.org MONOCLONAL ANTIBODY 24D2

Antibody name: 24D2 Antigen: CD340 (HER2, erbB2) Isotype: IgG1 Immunogen: NIH-3T3/huHER-2 cells Reactivity: Tumor cells, subset of ALL blasts, bone marrow MSC Reference: http://www.hcdm.org Bühring HJ, Sures I, Jallal B, Weiß U, Busch FW, Ludwig WD, Handgretinger R, Waller HD, Ullrich A. The receptor tyrosine kinase p185HER2 is expressed on a subset of B-lymphoid blasts from patients with acute lymphoblastic leukemia and chronic myelogenous leukemia. Blood. 1995;86:1916-1923.

MONOCLONAL ANTIBODY 28D4

Antibody name: 28D4 Antigen: CD140b (PDGF-RB) Isotype: IgG2b Immunogen: NIH-3T3/huPDGFRA cells Reactivity: bone marrow MSC Reference: Leucocyte Typing VIII; http://www.hcdm.org Bühring HJ, Battula VL, Treml S, Schewe B, Kanz L, Vogel W. Novel markers for the prospective isolation of human MSC. Ann New York Acad Sci 2007; 1106:262-71.

MONOCLONAL ANTIBODY 43A1

Antibody name: 43A1 Antigen: CD34 Isotype: IgG3 Immunogen: KG-1a cell line Reactivity: hematopoietic stem cells, endothelialial cells Reference: HLDA5 (Boston) MONOCLONAL ANTIBODY 43A3

Antibody name: 43A3 Antigen: human CD105 (endoglin) Isotype: IgG1 Immunogen: L-cells transfected with human CD105 Reactivity: immature erythroid cells, endothelial cells (all stages), mesenchymal cells. Reference: Bühring HJ, Müller C, Letarte M, Gougos A, Saalmüller A, Van Agthoven A, Busch FW. Endoglin is expressed on a subpopulation of immature erythroid cells of normal human bone marrow. Leukemia. 1991;5:841-847.

MONOCLONAL ANTIBODY 46A11

Antibody name: 46A11 Antigen: CD13 (aminopeptidase N) Isotype: IgG2b Immunogen: NIH-3T3 cells transfected with human CD13 Reactivity: Myeloid cells, MSC Reference: HLDA4 (Vienna, Austria)

MONOCLONAL ANTIBODY 48B3

Antibody name: 48B3 Antigen: CD167a (Discoidin domain receptor 1) Isotype: IgM Immunogen: NIH-3T3 cells transfected with human DDR1 (CD167a) Reactivity: Expression is found mainly in epithelial cells of various organs Reference: http://mpr.nci.nih.gov/prow/guide/1986613159_g.htm

Matsuyama W, Watanabe M, Shirahama Y, Oonakahara K, Higashimoto I, Yoshimura T, Osame M, Arimura K. Activation of discoidin domain receptor 1 on CD14-positive bronchoalveolar lavage fluid cells induces chemokine production in idiopathic pulmonary fibrosis. J Immunol. 2005;174:6490-8.

MONOCLONAL ANTIBODY 51D6

Antibody name: 51D6 Antigen: CD167a (Discoidin domain receptor 1) Isotype: IgM Immunogen: NIH-3T3 cells transfected with human DDR1 (CD167a) Reactivity: Expression is found mainly in epithelial cells of various organs Reference: http://mpr.nci.nih.gov/prow/guide/1986613159_g.htm

Matsuyama W, Watanabe M, Shirahama Y, Oonakahara K, Higashimoto I, Yoshimura T, Osame M, Arimura K. Activation of discoidin domain receptor 1 on CD14-positive bronchoalveolar lavage fluid cells induces chemokine production in idiopathic pulmonary fibrosis. J Immunol. 2005;174:6490-8.

MONOCLONAL ANTIBODY 57A5

Antibody name: 57A5 Antigen: human CD117 (c-kit) Isotype: IgG1 Immunogen: MOLM-1 megakaryocitic cell line Reactivity: Hematopoietic stem cells, mast cells Reference: HLDA-6, Myeloid Workshop (Kobe, Japan) Application: Suitable for immunohistochemistry MONOCLONAL ANTIBODY 57D2

Antibody name: 57D2 Antigen: not identified (Calsyntenin-3???) Isotype: IgG1 Immunogen: UT-7 erythroleukemia cell line Reactivity: Neural progenitor cells cells, NT-2 and T-47D cells Reference: Vogel W, Grünebach F, Messam CA, Kanz L, Brugger W, Bühring HJ. Heterogeneity among human bone marrow derived mesenchymal stem cells and neural progenitor cells. Haematologica. 2003;88:126-133.

MONOCLONAL ANTIBODY 58A2

Antibody name: 58A2 Antigen: not identified Isotype: IgG3 Immunogen: UT-7 erythroleukemia cell line Reactivity: NT-2 cells Reference: no reference

MONOCLONAL ANTIBODY 58B1

Antibody name: 58B1 Antigen: CD276 (B7-H3; checkpoint molecule) Isotype: IgG1 Immunogen: UT-7 erythroleukemia cell line Reactivity: Reacts with MSC in BM, activated dendritic cells and with almost all ested cell lines Reference: Bühring HJ, Battula VL, Treml S, Schewe B, Kanz L, Vogel W. Novel markers for the prospective isolation of human MSC. Ann New York Acad Sci. 2007;1106:262-271.

Tryfonidou MA, Schumann S, Armeanu S, Harichandan A, Sivasubramaniyan K, Mollenhauer J, Bühring HJ. Update on canine MSC markers. Cytometry Part A 2014;85:379-381. Autenrieth SA, Grimm S, Rittig SM, Frank Grünebach F, Gouttefangeas C, Bühring HJ. Profiling of primary peripheral blood- and -derived dendritic cells using monoclonal antibodies from the HLDA10 Workshop in Wollongong, Australia. Clin Trans Immunol. 2015;4:e50; doi: 101038/cti.2015.29.

MONOCLONAL ANTIBODY 58B3

Antibody name: 58B3 Antigen: not identified Isotype: Ig1 Immunogen: UT-7 erythroleukemic cell line Reactivity: Subsets of activated DCs Reference: Autenrieth SA, Grimm S, Rittig SM, Frank Grünebach F, Gouttefangeas C, Bühring HJ. Profiling of primary peripheral blood- and monocyte-derived dendritic cells using monoclonal antibodies from the HLDA10 Workshop in Wollongong, Australia. Clin Trans Immunol. 2015;4:e50; doi: 101038/cti.2015.29.

MONOCLONAL ANTIBODY 67A4

Antibody name: 67A4 Antigen: CD324 (E-cadherin) Isotype: IgG1 Immunogen: T47-D cell line Reactivity: Erythroblasts, Epithelial cells Reference: Armeanu S, Bühring HJ, Reuss-Borst M, Müller CA, Klein G. E-cadherin is functionally involved in the maturation of the erythroid lineage. J Cell Biol. 1995;131:243-249.

Bühring HJ, Müller T, Herbst R, Cole S, Rappold I, Schuller W, Zhu X, Fritzsche U, Faul C, Armeanu S, Ullrich A, Klein G, Schmidt H. The adhesion molecule E-cadherin and a surface antigen recognized by the antibody 9C4 are selectively expressed on erythroid cells of defined maturational stages. Leukemia. 1996;10:106-116.

HLDA8 (Adelaide, Australia)

MONOCLONAL ANTIBODY 67D2

Antibody name: 67D2 Antigen: CD164 (MGC-24, MUC-24) Isotype: IgG1 Immunogen: T47-D cell line Reactivity: Hematopoietic stem cells, leukemic cells. Basophil activation marker Reference: Watt SM, Bühring HJ, Rappold I, Chan YS, Lee-Prudhoe J, Jones T, Zannettino ACW, Simmons PJ, Doyonnas R, Sheer D, Butler L. CD164, a novel sialomucin on CD34+ and erythroid subsets, is located on human chromosome 6q21. Blood. 1998;92:849-866.

Zannettino ACW, Bühring HJ, Niutta S, Watt SM, Benton AM, Simmons PJ. The sialomucin CD164 (MGC-24v) is an adhesive glycoprotein expressed by human hematopoietic progenitors and bone marrow stromal cells that serves as a potent negative regulator of hematopoiesis. Blood. 1998;92:2613-2628.

Watt SM, Butler LH, Tavian M, Bühring HJ, Rappold I, Simmons PJ, Zannettino ACW, Buck D, Fuchs A, Doyonnas R, Chan JYH, Levesque JP, Peault B & Roxanis I: Functionally defined CD164 are expressed on CD34+ cells throughout ontogeny, but display distinct distribution patterns in adult hematopoietic and non-hematopoietic tissues. (2000) Blood 95:3113-3124.

Doyonnas R, Chan JYH, Butler LH, Rappold I, Lee-Prudhoe J, Zannettino ACW, Simmons PJ, Bühring HJ, Levesque JP & Watt SM: The first mucin domain of CD164 contains the epitopes for CD164 monoclonal antibodies that block hematopoietic stem cell adhesion and proliferation. (2000) J Immunol 165:840-851.

MONOCLONAL ANTIBODY 89B3

Antibody name: 89B3 Antigen: Monoclonal rat ant-mouse FLK-1 antibody Isotype: IgG2a Immunogen: rat-1/mu-FLK1 cell line Reactivity: Embryonic hematopoietic precursors Reference: Kabrun N, Bühring HJ, Choi K, Ullrich A, Risau W, Keller G. FLK-1 expression defines a unique population of embryonic hematopoietic precursors. Development. 1997;124:2039-2048.

MONOCLONAL ANTIBODY 97A6

Antibody name: 97A6 Antigen: CD203c (E-NPP3, ecto-nucleotide pyrophosphatase/phosphodiesterase 3) Isotype: IgG1 Immunogen: UT-7 cell line Reactivity: Basophils and mast cells. Basophil activation marker Reference: Bühring HJ, Simmons PJ, Pudney M, Müller M, Jarrossay D, van Agthoven A, Willheim M, Brugger W, Valent P & Kanz L: The monoclonal antibody 97A6 defines a novel surface antigen expressed on human basophils and their multi- and unipotent progenitors. (1999) Blood 94:2343-2356.

Bühring HJ, Seiffert M, Giesert C, Marxer A, Valent P, Kanz L, Sano K: The basophil activation marker defined by antibody 97A6 is identical with the ecto-nucleotide pyrophosphatase/phosphodiesterase 3 (E-NPP3; CD203c). (2001) Blood 97:3303-3305.

Platz IJ, Binder M, Marxer A, Lischka G, Valent P, Bühring HJ: Hymenoptera venom-induced upregulation of the basophil activation marker ecto-nucleotide pyrophosphatase/phosphodiesterase 3 (E-NPP3, CD203c) in sensitized individuals. (2001) Int Arch Allergy Immunol 126:335-342.

MONOCLONAL ANTIBODY 97C5

Antibody name: 97C5 Antigen: CD10 (CALLA) Isotype: IgG1 Immunogen: BV-173 leukemic cell line Reactivity: ALL blasts, B-progenitor cells, MSC Reference: HLDA 6 (Kobe, Japan)

Bühring HJ, Treml S, Cerabona F, de Zwart P, Kanz L, Sobiesiak M. Phenotypic characterization of distinct human bone marrow-derived MSC subsets. Hematopoietic Stem Cells VII: Ann NY Acad Sci 2009;1176:124-134

MONOCLONAL ANTIBODY 103B2

Antibody name: 103B2 Antigen: CD164 (MGC-24, MUC-24) Isotype: IgG3 Immunogen: MOLM-1 cell line Reactivity: Hematopoietic stem cells, leukemic cells. Basophil activation marker Reference: Watt SM, Bühring HJ, Rappold I, Chan YS, Lee-Prudhoe J, Jones T, Zannettino ACW, Simmons PJ, Doyonnas R, Sheer D, Butler L. CD164, a novel sialomucin on CD34+ and erythroid subsets, is located on human chromosome 6q21. Blood. 1998;92:849-866.

Zannettino ACW, Bühring HJ, Niutta S, Watt SM, Benton AM, Simmons PJ. The sialomucin CD164 (MGC-24v) is an adhesive glycoprotein expressed by human hematopoietic progenitors and bone marrow stromal cells that serves as a potent negative regulator of hematopoiesis. Blood. 1998;92:2613-2628.

Watt SM, Butler LH, Tavian M, Bühring HJ, Rappold I, Simmons PJ, Zannettino ACW, Buck D, Fuchs A, Doyonnas R, Chan JYH, Levesque JP, Peault B & Roxanis I: Functionally defined CD164 epitopes are expressed on CD34+ cells throughout ontogeny, but display distinct distribution patterns in adult hematopoietic and non-hematopoietic tissues. (2000) Blood 95:3113-3124.

Doyonnas R, Chan JYH, Butler LH, Rappold I, Lee-Prudhoe J, Zannettino ACW, Simmons PJ, Bühring HJ, Levesque JP & Watt SM: The first mucin domain of CD164 contains the epitopes for CD164 monoclonal antibodies that block hematopoietic stem cell adhesion and proliferation. (2000) J Immunol 165:840-851.

MONOCLONAL ANTIBODY 104D2

Antibody name: 104D2 Antigen: human CD117 (c-kit) Isotype: IgG1 Immunogen: MOLM-1 megakaryocitic cell line Reactivity: Hematopoietic stem cells, mast cells Reference: Ashman LK, Cambareri AC, Ngoyen L, Bühring HJ. CD117 (c-kit) panel report. In: Leucocyte Typing VI. White Cell Differentiation Antigens. Kishimoto T, Kikutani H, von dem Borne AEGKr, Goyert SM, Mason DY, Miyasaka M, Moretta L, Okumura K, Shaw S, Springer TA, Sugamura K & Zola H (eds), Garland Publishing, Inc. New York. 1998; pp 816-818.

MONOCLONAL ANTIBODY 105A5

Antibody name: 105A5 Antigen: CD164 (MGC-24, MUC-24) Isotype: IgM Immunogen: MOLM-1 cell line Reactivity: Hematopoietic stem cells, leukemic cells. Basophil activation marker Reference: Watt SM, Bühring HJ, Rappold I, Chan YS, Lee-Prudhoe J, Jones T, Zannettino ACW, Simmons PJ, Doyonnas R, Sheer D, Butler L. CD164, a novel sialomucin on CD34+ and erythroid subsets, is located on human chromosome 6q21. Blood. 1998;92:849-866.

Zannettino ACW, Bühring HJ, Niutta S, Watt SM, Benton AM, Simmons PJ. The sialomucin CD164 (MGC-24v) is an adhesive glycoprotein expressed by human hematopoietic progenitors and bone marrow stromal cells that serves as a potent negative regulator of hematopoiesis. Blood. 1998;92:2613-2628.

Watt SM, Butler LH, Tavian M, Bühring HJ, Rappold I, Simmons PJ, Zannettino ACW, Buck D, Fuchs A, Doyonnas R, Chan JYH, Levesque JP, Peault B & Roxanis I: Functionally defined CD164 epitopes are expressed on CD34+ cells throughout ontogeny, but display distinct distribution patterns in adult hematopoietic and non-hematopoietic tissues. (2000) Blood 95:3113-3124.

Doyonnas R, Chan JYH, Butler LH, Rappold I, Lee-Prudhoe J, Zannettino ACW, Simmons PJ, Bühring HJ, Levesque JP & Watt SM: The first mucin domain of CD164 contains the epitopes for CD164 monoclonal antibodies that block hematopoietic stem cell adhesion and proliferation. (2000) J Immunol 165:840-851.

MONOCLONAL ANTIBODY 293-C3

Antibody name: 293-C3 Antigen: CD133 (Prominin) Isotype: Ig2b Immunogen: HEK-293 cells transfected with full-length uman CD133 (HEK-293/huCD133) Reactivity: Hematopoietic, neural, endothelal stem cells, epithelial cells. Reference: HLDA 8 (Adelaide, Australia)

MONOCLONAL ANTIBODY A3C6

Antibody name: A3C6 Antigen: human CD117 (c-kit) Isotype: IgG1 Immunogen: MOLM-1 megakaryocitic cell line Reactivity: Hematopoietic stem cells, mast cells Reference: HLDA-6 (Kobe, Japan) Application: A3C6 potently blocks the binding of its ligand SCF to the receptor! MONOCLONAL ANTIBODY B1D5

Antibody name: B1D5 Antigen: CD172b (SIRPβ) Isotype: IgG2a Immunogen: GST-SIRPβ (fusion protein) Reactivity: , , dendritic cells Reference: Seiffert M, Brossart P, Cant C, Cella M, Colonna M, Brugger W, Kanz L, Ullrich A, Bühring HJ. Signal-regulatory protein (SIRP)α but not SIRPβ is involved in activation, binds to CD47 with high affinity, and is expressed on immature CD34+CD38- hematopoietic cells. Blood. 2001;97:2741-2749.

Motegi S, Okazawa H, Satoh R, Ohnishi H, Kaneko Y, kobayashi H, Tomizawa K, Ito T, Honma N, Bühring HJ, Ishikawa O, Matozaki T. Roles of the CD47-SHPS-1 system in regulation of cell migration: Involvement of SHP-2 protein tyrosine phosphatase. EMBO J. 2003;22:2634-2644.

MONOCLONAL ANTIBODY B4B6

Antibody name: B4B6 Antigen: CD172b (SIRPβ) Isotype: IgG1 Immunogen: NIH-3T3/hu-SIRPbeta cell line Reactivity: monocytes, granulocytes, dendritic cells Reference: Seiffert M, Brossart P, Cant C, Cella M, Colonna M, Brugger W, Kanz L, Ullrich A, Bühring HJ. Signal-regulatory protein (SIRP)α but not SIRPβ is involved in T cell activation, binds to CD47 with high affinity, and is expressed on immature CD34+CD38- hematopoietic cells. Blood. 2001;97:2741-2749.

Motegi S, Okazawa H, Satoh R, Ohnishi H, Kaneko Y, kobayashi H, Tomizawa K, Ito T, Honma N, Bühring HJ, Ishikawa O, Matozaki T. Roles of the CD47-SHPS-1 system in regulation of cell migration: Involvement of SHP-2 protein tyrosine phosphatase. EMBO J. 2003;22:2634-2644.

MONOCLONAL ANTIBODY BV10

Antibody name: BV10 Antigen: human CD135 (FLT3) Isotype: IgG1 Immunogen: BaF3/hu-FLT3 cell line Reactivity: Subset of stem cells, subsets of leukemic blasts. Reference: HLDA6 (Kobe): Bühring HJ, Birnbaum D, Brasel K, Civin CI, Götze K, Lyman S, Rappold I, Rosnet O. CD135 workshop panel report. In: Leucocyte Typing VI. White Cell Differentiation Antigens. Kishimoto T, Kikutani H, von dem Borne AEGKr, Goyert SM, Mason DY, Miyasaka M, Moretta L, Okumura K, Shaw S, Springer TA, Sugamura K & Zola H (eds), Garland Publishing, Inc, New York. 1998; pp 875-79

Rappold I, Ziegler BL, Köhler I, Marchetto, S, Rosnet O, Birnbaum D, Simmons PJ, Zannettino ACW, Hill B, Neu S, Knapp W, Alitalo R, Alitalo K, Ullrich A, Kanz L, Bühring HJ. Functional and phenotypic characterization of cord blood and bone marrow subsets expressing FLT3 receptor tyrosine kinase. Blood. 1997;90:111-125.

MONOCLONAL ANTIBODY CC2C6

Antibody name: CC2C6 Antigen: CD47 (integrin-associated protein IAP, Ovarian carcinoma antigen OA3, Rh-associated protein, MEM-133) Isotype: IgG1 Immunogen: CCRF-CEM T-cell line Reactivity: ubiquitous Reference: Seiffert M, Cant C, Chen Z, Rappold I, Brugger W, Kanz L, Brown EJ, Ullrich A & Bühring HJ: Human Signal- Regulatory Protein is expressed on normal but not on subsets of leukemic myeloid cells and mediates cellular adhesion involving its counter-receptor CD47. (1999) Blood 94:3633- 3643. Reark: Binds to CD172a

MONOCLONAL ANTIBODY CH1A4

Antibody name: CH1A4 Antigen: not identified Isotype: IgG3 Immunogen: WERI-RB-1 cell line Reactivity: NCCIT subline of NT-2 teratocarcimoma cells Reference: no reference

MONOCLONAL ANTIBODY CH2A3

Antibody name: CH2A3 Antigen: not identified Isotype: IgG2a Immunogen: WERI-RB-1 cell line Reactivity: NCCIT subline of NT-2 teratocarcimoma cells Reference: no reference

MONOCLONAL ANTIBODY CH2A4

Antibody name: CH2A4 Antigen: not identified Isotype: IgG3 Immunogen: WERI-RB-1 cell line Reactivity: NCCIT subline of NT-2 teratocarcimoma cells Reference: no reference

MONOCLONAL ANTIBODY CH3A4A7

Antibody name: CH3A4A7 Antigen: CD344 (frizzled-4) Isotype: IgG1 Immunogen: WERI-RB-1 cell line Reactivity: neuronal progenitor cells, enteric nervous system Reference: Nothelfer K, Obermayr F, Belz N, Reinartz E, Bareiss PM, Bühring HJ, Beschorner R. Just L. Expression of the Wnt receptor frizzled-4 in human enteric nervous system of infants. Stem Cells International 2016 ID 9076823. http://dx.doi.org/10.1155/2016/9076823

MONOCLONAL ANTIBODY CH4D3

Antibody name: CH4D3 Antigen: not identified Isotype: IgG2b Immunogen: WERI-RB-1 cell line Reactivity: NCCIT subline of NT-2 teratocarcimoma cells Reference: no reference

MONOCLONAL ANTIBODY CUB1

Antibody name: CUB1 Antigen: CD318 (CUB domain containing protein 1, CDCP1) Isotype: IgG2b Immunogen: NIH-3T3/huCDCP1 Reactivity: Tumor cells, hematopoietic stem cells Reference: Bühring HJ, Kući S, Conze T, Rathke G, Bartolović K, Grünebach F, Scherl-Mostageer M, Brümmendorf T, Schweifer N, Lammers R. CDCP1 identifies a broad spectrum of normal and malignant stem/progenitor cell subsets of hematopoietic and non-hematopoietic origin. Stem Cells. 2004;22:334-343.

MONOCLONAL ANTIBODY CUB2

Antibody name: CUB2 Antigen: CD318 (CUB domain containing protein 1, CDCP1) Isotype: IgG2a Immunogen: NIH-3T3/huCDCP1 Reactivity: Tumor cells, hematopoietic stem cells Reference: Bühring HJ, Kući S, Conze T, Rathke G, Bartolović K, Grünebach F, Scherl-Mostageer M, Brümmendorf T, Schweifer N, Lammers R. CDCP1 identifies a broad spectrum of normal and malignant stem/progenitor cell subsets of hematopoietic and non-hematopoietic origin. Stem Cells. 2004;22:334-343.

MONOCLONAL ANTIBODY CUB4

Antibody name: CUB4 Antigen: CD318 (CUB domain containing protein 1, CDCP1) Isotype: IgG2a Immunogen: NIH-3T3/huCDCP1 Reactivity: Tumor cells, hematopoietic stem cells Reference: Bühring HJ, Kući S, Conze T, Rathke G, Bartolović K, Grünebach F, Scherl-Mostageer M, Brümmendorf T, Schweifer N, Lammers R. CDCP1 identifies a broad spectrum of normal and malignant stem/progenitor cell subsets of hematopoietic and non-hematopoietic origin. Stem Cells. 2004;22:334-343.

MONOCLONAL ANTIBODY DOR5A2B4

Antibody name: DOR5A2B4 Antigen: δ-Opioid receptor Isotype: IgG2b Immunogen: HEK-293/hu-δ-Opioid receptor Reactivity: δ-Opioid receptor-positive neural cells, tumor cells Reference: no reference

MONOCLONAL ANTIBODY DOR5B2D9

Antibody name: DOR5B2D9 Antigen: δ-Opioid receptor Isotype: IgG2b Immunogen: HEK-293/hu-δ-Opioid receptor Reactivity: δ-Opioid receptor-positive neural cells, tumor cells Reference: no reference

MONOCLONAL ANTIBODY DOR7D2A4

Antibody name: DOR7D2A4 Antigen: δ-Opioid receptor Isotype: IgG2b Immunogen: HEK-293/hu-δ-Opioid receptor Reactivity: δ-Opioid receptor-positive neural cells, tumor cells Reference: no reference

MONOCLONAL ANTIBODY F9-3C2F1

Antibody name: F9-3C2F1 Antigen: not identified Isotype: IgM Immunogen: HEK293 embryonioc kidney cells Reactivity: tumor cells, MSC subpopulation Reference: Bühring HJ, Treml S, Cerabona F, de Zwart P, Kanz L, Sobiesiak M. Phenotypic characterization of distinct human bone marrow-derived MSC subsets. Hematopoietic Stem Cells VII: Ann NY Acad Sci 2009;1176:124-134

Bühring HJ, Battula VL, Treml S, Schewe B, Kanz L, Vogel W. Novel markers for the prospective isolation of human MSC. Ann New York Acad Sci 2007; 1106:262-71.

Autenrieth SA, Grimm S, Rittig SM, Frank Grünebach F, Gouttefangeas C, Bühring HJ. Profiling of primary peripheral blood- and monocyte-derived dendritic cells using monoclonal antibodies from the HLDA10 Workshop in Wollongong, Australia. Clin Trans Immunol. 2015;4:e50; doi: 101038/cti.2015.29.

MONOCLONAL ANTIBODY HEK-3D6

Antibody name: HEK-3D6 Antigen: not identified Isotype: IgG2a Immunogen: HEK embryonic kidney cells Reactivity: Bone marrow-derived MSC and CD34+ HSC Reference: Bühring HJ, Treml S, Cerabona F, de Zwart P, Kanz L, Sobiesiak M. Phenotypic characterization of distinct human bone marrow-derived MSC subsets. Hematopoietic Stem Cells VII: Ann NY Acad Sci 2009;1176:124-134

Bühring HJ, Battula VL, Treml S, Schewe B, Kanz L, Vogel W. Novel markers for the prospective isolation of human MSC. Ann New York Acad Sci 2007; 1106:262-71.

MONOCLONAL ANTIBODY HEK5-1B3A3

Antibody name: HEK5-1B3A3 Antigen: CD276 (B7-H3; checkpoint molecule) Isotype: IgG1 Immunogen: HEK293 embryonic kidney cells Reactivity: Reacts with MSC in BM and with almost all tested cell lines Reference: Tryfonidou MA, Schumann S, Armeanu S, Harichandan A, Sivasubramaniyan K, Mollenhauer J, Bühring HJ. Update on canine MSC markers. Cytometry Part A 2014;85:379-381.

MONOCLONAL ANTIBODY NP4D6

Antibody name: NP4D6 Antigen: CD203c (E-NPP3, ecto-nucleotide pyrophosphatase/phosphodiesterase 3) Isotype: IgG1 Immunogen: HEK-293/hu-E-NPP3 cells Reactivity: Basophils and mast cells. Basophil activation marker Reference: HLDA 8 (Adelaide, Australia)

MONOCLONAL ANTIBODY P3C4

Antibody name: P3C4 Antigen: CD172a (SIRPα) Isotype: IgG2a Immunogen: NIH-3T3/hu-SIRPα Reactivity: Stem cells, myeloid cells, MSC Remark: CD172a binds to CD47 Reference: Seiffert M, Brossart P, Cant C, Cella M, Colonna M, Brugger W, Kanz L, Ullrich A, Bühring HJ. Signal-regulatory protein (SIRP)α but not SIRPβ is involved in T cell activation, binds to CD47 with high affinity, and is expressed on immature CD34+CD38- hematopoietic cells. Blood. 2001;97:2741-2749.

Seiffert M, Cant C, Chen Z, Rappold I, Brugger W, Kanz L, Brown EJ, Ullrich A, Bühring HJ. Human Signal-Regulatory Protein is expressed on normal but not on subsets of leukemic myeloid cells and mediates cellular adhesion involving its counter-receptor CD47. Blood. 1999;94:3633-3643.

MONOCLONAL ANTIBODY SE5A5

Antibody name: SE5A5 Antigen: CD172a (SIRPα) Isotype: IgG1 Immunogen: NIH-3T3/hu-SIRPα Reactivity: Stem cells, myeloid cells, MSC Remark: CD172a binds to CD47 Reference: Seiffert M, Brossart P, Cant C, Cella M, Colonna M, Brugger W, Kanz L, Ullrich A, Bühring HJ. Signal-regulatory protein (SIRP)α but not SIRPβ is involved in T cell activation, binds to CD47 with high affinity, and is expressed on immature CD34+CD38- hematopoietic cells. Blood. 2001;97:2741-2749.

Seiffert M, Cant C, Chen Z, Rappold I, Brugger W, Kanz L, Brown EJ, Ullrich A, Bühring HJ. Human Signal-Regulatory Protein is expressed on normal but not on subsets of leukemic myeloid cells and mediates cellular adhesion involving its counter-receptor CD47. Blood. 1999;94:3633-3643.

MONOCLONAL ANTIBODY SE7C2

Antibody name: SE7C2 Antigen: CD172a (SIRPα) Isotype: IgG1 Immunogen: NIH-3T3/hu-SIRPα Reactivity: Stem cells, myeloid cells, MSC Remark: CD172a binds to CD47 Reference: Seiffert M, Brossart P, Cant C, Cella M, Colonna M, Brugger W, Kanz L, Ullrich A, Bühring HJ. Signal-regulatory protein (SIRP)α but not SIRPβ is involved in T cell activation, binds to CD47 with high affinity, and is expressed on immature CD34+CD38- hematopoietic cells. Blood. 2001;97:2741-2749.

Seiffert M, Cant C, Chen Z, Rappold I, Brugger W, Kanz L, Brown EJ, Ullrich A, Bühring HJ. Human Signal-Regulatory Protein is expressed on normal but not on subsets of leukemic myeloid cells and mediates cellular adhesion involving its counter-receptor CD47. Blood. 1999;94:3633-3643.

MONOCLONAL ANTIBODY SE8A3

Antibody name: SE8A3 Antigen: CD172a (SIRPα) Isotype: IgG1 Immunogen: NIH-3T3/hu-SIRPα Reactivity: Stem cells, myeloid cells, MSC Remark: CD172a binds to CD47 Reference: Seiffert M, Brossart P, Cant C, Cella M, Colonna M, Brugger W, Kanz L, Ullrich A, Bühring HJ. Signal-regulatory protein (SIRP)α but not SIRPβ is involved in T cell activation, binds to CD47 with high affinity, and is expressed on immature CD34+CD38- hematopoietic cells. Blood. 2001;97:2741-2749.

Seiffert M, Cant C, Chen Z, Rappold I, Brugger W, Kanz L, Brown EJ, Ullrich A, Bühring HJ. Human Signal-Regulatory Protein is expressed on normal but not on subsets of leukemic myeloid cells and mediates cellular adhesion involving its counter-receptor CD47. Blood. 1999;94:3633-3643.

MONOCLONAL ANTIBODY SE12B6

Antibody name: SE12B6 Antigen: CD172a (SIRPα) Isotype: IgG1 Immunogen: NIH-3T3/hu-SIRPα Reactivity: Stem cells, myeloid cells, MSC Remark: CD172a binds to CD47 Reference: Seiffert M, Brossart P, Cant C, Cella M, Colonna M, Brugger W, Kanz L, Ullrich A, Bühring HJ. Signal-regulatory protein (SIRP)α but not SIRPβ is involved in T cell activation, binds to CD47 with high affinity, and is expressed on immature CD34+CD38- hematopoietic cells. Blood. 2001;97:2741-2749.

Seiffert M, Cant C, Chen Z, Rappold I, Brugger W, Kanz L, Brown EJ, Ullrich A, Bühring HJ. Human Signal-Regulatory Protein is expressed on normal but not on subsets of leukemic myeloid cells and mediates cellular adhesion involving its counter-receptor CD47. Blood. 1999;94:3633-3643.

MONOCLONAL ANTIBODY SE12C3

Antibody name: SE12C3 Antigen: CD172a (SIRPα) Isotype: IgG1 Immunogen: NIH-3T3/hu-SIRPα Reactivity: Stem cells, myeloid cells, MSC Remark: CD172a binds to CD47 Reference: Seiffert M, Brossart P, Cant C, Cella M, Colonna M, Brugger W, Kanz L, Ullrich A, Bühring HJ. Signal-regulatory protein (SIRP)α but not SIRPβ is involved in T cell activation, binds to CD47 with high affinity, and is expressed on immature CD34+CD38- hematopoietic cells. Blood. 2001;97:2741-2749.

Seiffert M, Cant C, Chen Z, Rappold I, Brugger W, Kanz L, Brown EJ, Ullrich A, Bühring HJ. Human Signal-Regulatory Protein is expressed on normal but not on subsets of leukemic myeloid cells and mediates cellular adhesion involving its counter-receptor CD47. Blood. 1999;94:3633-3643.

MONOCLONAL ANTIBODY W1C3

Antibody name: W1C3 Antigen: CD56 (NCAM) Isotype: IgG1 Immunogen: WEII-RB-1 retinoblastoma cell line Reactivity: NK cells, MSC subset Reference: Bühring HJ, Treml S, Cerabona F, de Zwart P, Kanz L, Sobiesiak M. Phenotypic characterization of distinct human bone marrow-derived MSC subsets. Hematopoietic Stem Cells VII: Ann NY Acad Sci 2009;1176:124-134

Bühring HJ, Battula VL, Treml S, Schewe B, Kanz L, Vogel W. Novel markers for the prospective isolation of human MSC. Ann New York Acad Sci 2007; 1106:262-71.

MONOCLONAL ANTIBODY W3A4

Antibody name: W3A4 Antigen: not identified Isotype: IgG1 Immunogen: WERI-RB-1 retinoblastoma cells Reactivity: retinoblastoma cells Reference: no reference

MONOCLONAL ANTIBODY W3C3

Antibody name: W3C3 Antigen: not identified Isotype: IgG1 Immunogen: WERI-RB-1 retinoblastoma cells Reactivity: Neuronal progenitor cells, retinoblastoma cells Reference: no reference MONOCLONAL ANTIBODY W3C4

Antibody name: W3C4 Antigen: CD349 (frizzled-9) Isotype: IgM Immunogen: WEII-RB-1 retinoblastoma cell line Reactivity: Primary MSC of placenta and bone marrow Reference: http://www.hcdm.org

Battula VL, Bareiß P, Treml S, Conrad S, Albert I, Hojak S, Abele H, Schewe B, Just L, Skutella T, Bühring HJ. Placenta and bone marrow derived MSC cultured in serum-free, b-FGF containing medium express cell surface Frizzled-9 and SSEA-4 and give rise to multi-lineage differentiation. Differentiation. 2007;75:279-291.

Bühring HJ, Battula VL, Treml S, Schewe B, Kanz L, Vogel W. Novel markers for the prospective isolation of human MSC. Ann New York Acad Sci 2007; 1106:262-71.

Battula VL, Treml S, Abele H, Bühring HJ. Prospective isolation and characterization of mesenchymal stem cells from human placenta using a frizzled-9-specific monoclonal antibody. Differentiation 2008;1106:326.336.

MONOCLONAL ANTIBODY W3D2

Antibody name: W3D2 Antigen: not identified Isotype: IgG1 Immunogen: WERI-RB-1 retinoblastoma cells Reactivity: retinoblastoma cells Reference: no reference

MONOCLONAL ANTIBODY W3D5

Antibody name: W3D5 Antigen: SUSD2 (sushi domain containing 2) Isotype: IgG2a Immunogen: WERI-RB-1 retinoblastoma cells Reactivity: retinoblastoma cells, primary bone marrow-derived MSC Reference: Bühring HJ, Battula VL, Treml S, Schewe B, Kanz L, Vogel W. Novel markers for the prospective isolation of human MSC. Ann New York Acad Sci 2007; 1106:262-71.

Sivasubramaniyan K, Harichandan A, Schumann S, Sobiesiak M, Lengerke C, Maurer A, Kalbacher H, Bühring HJ. Prospective isolation of mesenchymal stem cells from human bone marrow using novel antibodies directed against Sushi domain containing 2. Stem Cells Dev. 2013;22:1944-54.

MONOCLONAL ANTIBODY W4A5

Antibody name: W4A5 Antigen: not identified (Calsyntenin-3???) Isotype: IgG1 Immunogen: WEII-RB-1 retinoblastoma cell line Reactivity: Neural progenitor cells, bone marrow MSC subset Reference: Bühring HJ, Treml S, Cerabona F, de Zwart P, Kanz L, Sobiesiak M. Phenotypic characterization of distinct human bone marrow-derived MSC subsets. Hematopoietic Stem Cells VII: Ann NY Acad Sci 2009;1176:124-134

Bühring HJ, Battula VL, Treml S, Schewe B, Kanz L, Vogel W. Novel markers for the prospective isolation of human MSC. Ann New York Acad Sci 2007; 1106:262-71.

MONOCLONAL ANTIBODY W5C4

Antibody name: W5C4 Antigen: not identified Isotype: IgG1 Immunogen: WERI-RB-1 retinoblastoma cells Reactivity: Retinoblastoma cells, Primary bone marrow MSC. Dendritic cells Reference: Bühring HJ, Battula VL, Treml S, Schewe B, Kanz L, Vogel W. Novel markers for the prospective isolation of human MSC. Ann New York Acad Sci 2007; 1106:262-71.

Bühring HJ, Treml S, Cerabona F, de Zwart P, Kanz L, Sobiesiak M. Phenotypic characterization of distinct human bone marrow- derived MSC subsets. Hematopoietic Stem Cells VII: Ann NY Acad Sci 2009;1176:124-134

Autenrieth SA, Grimm S, Rittig SM, Frank Grünebach F, Gouttefangeas C, Bühring HJ. Profiling of primary peripheral blood- and monocyte-derived dendritic cells using monoclonal antibodies from the HLDA10 Workshop in Wollongong, Australia. Clin Trans Immunol. 2015;4:e50; doi: 101038/cti.2015.29.

MONOCLONAL ANTIBODY W5C5

Antibody name: W5C5 Antigen: SUSD2 (sushi domain containing 2) Isotype: IgG1 Immunogen: WERI-RB-1 retinoblastoma cells Reactivity: retinoblastoma cells, primary bone marrow-derived MSC Reference: Bühring HJ, Battula VL, Treml S, Schewe B, Kanz L, Vogel W. Novel markers for the prospective isolation of human MSC. Ann New York Acad Sci 2007; 1106:262-71.

Sivasubramaniyan K, Harichandan A, Schumann S, Sobiesiak M, Lengerke C, Maurer A, Kalbacher H, Bühring HJ. Prospective isolation of mesenchymal stem cells from human bone marrow using novel antibodies directed against Sushi domain containing 2. Stem Cells Dev. 2013;22:1944-54.

MONOCLONAL ANTIBODY W5D3

Antibody name: W5D3 Antigen: not identified Isotype: IgG1 Immunogen: WERI-RB-1 retinoblastoma cells Reactivity: Retinoblastoma cells, neuronal progenitor cells, many tumor cell lines Reference: no reference

MONOCLONAL ANTIBODY W6B3C1

Antibody name: W6B3C1 Antigen: CD133 (Prominin) Isotype: Ig1 Immunogen: WERI-RB-1 retinoblastoma cell line Reactivity: Hematopoietic, neural, endothelal stem cells, epithelial cells. Reference: HLDA7 in Harrogate. Bühring HJ, Marxer A, Lammers R, Wissinger B. CD133 cluster report. In: Leucocyte Typing VII. White Cell Differentiation Antigens. Mason D, André P, Bensussan A, Buckley C, Civin C, Clark E, de Haas M, Goyert S, Hadam M, Hart D, Hořejší V, Jones Y, Meuer S, Morrissey J, Schwartz-Albiez R, Shaw S, Simmons D, Turni L, Uguccioni M, van der Schoot E, Vivier E, Zola H (eds). Oxford University Press, Oxford. 2002; pp. 622-623. MONOCLONAL ANTIBODY W6D3

Antibody name: W6D3 Antigen: CD15 (Lewis X, X-) Isotype: Ig1 Immunogen: WERI-RB-1 retinoblastoma cell line Reactivity: , , neural progenitor cells. Reference: HLDA7 in Harrogate. Bühring HJ, Marxer A, Lammers R, Wissinger B. CD133 cluster report. In: Leucocyte Typing VII. White Cell Differentiation Antigens. Mason D, André P, Bensussan A, Buckley C, Civin C, Clark E, de Haas M, Goyert S, Hadam M, Hart D, Hořejší V, Jones Y, Meuer S, Morrissey J, Schwartz-Albiez R, Shaw S, Simmons D, Turni L, Uguccioni M, van der Schoot E, Vivier E, Zola H (eds). Oxford University Press, Oxford. 2002; pp. 622-623. MONOCLONAL ANTIBODY W7C5

Antibody name: W7C5 Antigen: CD109 (Platelet activation factor) Isotype: Ig1 Immunogen: WERI-RB-1 retinoblastoma cell line Reactivity: MSC and HSC subsets, megakaryocytes, subset of activated T cells Reference: Giesert C, Marxer A, Sutherland DR, Schuh AC, Kanz L, Bühring HJ. Antibody W7C5 defines a CD109 expressed on CD34+ and CD34- hematopoietic and mesenchymal stem cell subsets. In: Hematopoietic stem cells 2002: Genetics and Function. Orlic D, Brümmendorf TH, Sharkis SJ, Kanz L (eds). Ann New York Acad Sci. 2003;996:227-230. MONOCLONAL ANTIBODY W7C6

Antibody name: W7C6 Antigen: LAR (Leucocyte associated antigen; to be confirmed!) Isotype: Ig1 Immunogen: WERI-RB-1 retinoblastoma cell line Reactivity: Primary bone marrow-derived MSC Reference: Bühring HJ, Battula VL, Treml S, Schewe B, Kanz L, Vogel W. Novel markers for the prospective isolation of human MSC. Ann New York Acad Sci. 2007;1106:262-271. MONOCLONAL ANTIBODY W8B2B10

Antibody name: W8B2B10 Antigen: TNAP (Tissue non-specific alkaline phosphatase) Isotype: Ig1 Immunogen: WERI-RB-1 retinoblastoma cell line Reactivity: Primary bone marrow-derived MSC, ES and iPS cells Reference: Bühring HJ, Battula VL, Treml S, Schewe B, Kanz L, Vogel W. Novel markers for the prospective isolation of human MSC. Ann New York Acad Sci. 2007;1106:262-271.

Vogel W, Grünebach F, Messam CA, Kanz L, Brugger W, Bühring HJ. Heterogeneity among human bone marrow derived mesenchymal stem cells and neural progenitor cells. Haematologica. 2003;88:126-133.

Sobiesiak M, Sivasubramaniyan K, Hermann C, Tan C, Orgel M, Treml S, Cerabona F, de Zwart P, Ochs U, Müller CA, Gargett CE, Kalbacher H, Bühring HJ. The mesenchymal stem cell antigen MSCA-1 is identical to tissue non-specific alkaline phosphatase. Stem Cells Dev. 2010;19(5):669-77.

MONOCLONAL ANTIBODY W8C3

Antibody name: W8C3 Antigen: not identified Isotype: Ig1 Immunogen: WERI-RB-1 retinoblastoma cell line Reactivity: Subsets of cultured bone marrow-derived MSC Reference: no reference