Esteroides Anabolizantes
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Expression of Recombinant Human Androgen Receptor and Its Use for Screening Methods
Institut für Physiologie FML Weihenstephan Technische Universität München Expression of recombinant human androgen receptor and its use for screening methods Ellinor Rose Sigrid Bauer Vollständiger Abdruck der von der Fakultät Wissenschaftszentrum Weihenstephan für Ernährung, Landnutzung und Umwelt der Technischen Universität München zur Erlangung des akademischen Grades eines Doktors der Naturwissenschaften genehmigten Dissertation. Vorsitzender: Univ.-Prof. Dr. B. Hock Prüfer der Dissertation: Univ.-Prof. Dr. H. H. D. Meyer Univ.-Prof. Dr. H. Sauerwein (Rheinische Friedrich-Wilhelms-Universität Bonn) Die Dissertation wurde am 31.10.2002 bei der Technischen Universität München eingereicht und durch die Fakultät Wissenschaftszentrum Weihenstephan für Ernährung, Landnutzung und Umwelt am 03.12.2002 angenommen. Introduction Content 1. INTRODUCTION ..................................................................................................................................... 5 1.1. ENDOCRINE DISRUPTERS 5 1.2. ANDROGENS AND ANTIANDROGENS 7 1.2.1. DEFINITIONS 7 1.2.2. MODE OF ACTION 8 1.3. STRUCTURES OF ENDOCRINE DISRUPTERS 10 1.4. STRATEGIES FOR MONITORING ANDROGEN ACTIVE SUBSTANCES 13 1.4.1. IN VIVO METHODS 13 1.4.2. IN VITRO METHODS 15 1.5. OBJEKTIVE OF THE STUDIES 18 2. MATERIALS AND METHODS ................................................................................................................. 19 2.1. PREPARATION OF RECEPTORS 19 2.2. ASSAY SYSTEMS 19 2.2.1. IN SOLUTION AR ASSAY 19 2.2.2. IMMUNO-IMMOBILISED RECEPTOR ASSAY (IRA) 20 2.2.3. PR AND SHBG ASSAYS 21 2.2.4. DATA EVALUATION 21 2.3. ANALYTES 22 3. RESULTS AND DISCUSSION ................................................................................................................. 23 3.1. DEVELOPMENT OF NEW ASSAY SYSTEMS 23 3.1.1. BAR ASSAY 23 3.1.2. CLONING OF THE HUMAN AR AND PRODUCTION OF FUNCTIONAL PROTEIN 24 3.1.3. DEVELOPMENT OF A SCREENING ASSAY ON MICROTITRE PLATES (IRA) 25 3.2. -
Sportsletter Interview: Shaun Assael
SportsLetter Interviews December 2007 Volume 18, No. 6 Shaun Assael With the recent release of the Mitchell Report, the story of steroids in Major League Baseball has dominated sports coverage. The report states, “Everyone involved in baseball over the past two decades — Commissioners, club officials, the Players Association, and the players — shares to some extent in the responsibility for the steroids era. There was a collective failure to recognize the problem as it emerged and deal with it early on.” For all the hand-wringing about Major League Baseball’s mea culpa, the use of anabolic steroids and other performance- enhancing drugs has been sports’ dirty little not-so-secret for decades. In his book, “Steroid Nation: Juiced Home Run Totals, Anti-aging Miracles, and a Hercules in Every High School: The Secret History of America’s True Drug Addiction” (ESPN Books), ESPN staff writer Shaun Assael traces the culture of steroids in sports. The tale is about as long as the sub-title of the book, and Assael chronicles the many twists of this complex story. He writes about the long-forgotten “visionaries” (like Dan Duchaine, author of the “Underground Steroid Handbook”), the athletes who became caught in cycles of steroid abuse (NFL star Lyle Alzado) and the research scientists charged to nab them (UCLA’s Dr. Don Catlin). The result is a panoramic view of steroids in America — a view that echoes the Mitchell Report in placing responsibility for the spread of performance-enhancing drugs on just about everyone including the media 1 ©1996-2008 LA84 Foundation. Reproduction of SportsLetter is encouraged with credit to the LA84 Foundation. -
5 Part 1300—Definitions
PART 1300—DEFINITIONS (7) 5-androstenediol (3b,17b-dihydroxy- androst-5-ene) Sec. (8) 1-androstenedione ([5a]-androst-1- 1300.01 Definitions relating to controlled en-3,17-dione) substances. (9) 4-androstenedione (androst-4-en- 1300.02 Definitions relating to listed chemi- 3,17-dione) cals. (10) 5-androstenedione (androst-5-en- 1300.03 Definitions relating to electronic or- 3,17-dione) ders for controlled substances and elec- tronic prescriptions for controlled sub- (11) bolasterone (7a,17a-dimethyl-17b- stances. hydroxyandrost-4-en-3-one) 1300.04 Definitions relating to the dis- (12) boldenone (17b-hydroxyandrost-1,4- pensing of controlled substances by diene-3-one) means of the Internet. (13) boldione (androsta-1,4-diene-3,17- 1300.05 Definitions relating to the disposal dione) of controlled substances. (14) calusterone (7b,17a-dimethyl-17b- AUTHORITY: 21 U.S.C. 802, 821, 822, 829, hydroxyandrost-4-en-3-one) 871(b), 951, 958(f). (15) clostebol (4-chloro-17b- SOURCE: 62 FR 13941, Mar. 24, 1997, unless hydroxyandrost-4-en-3-one) otherwise noted. (16) dehydrochloromethyltestosterone (4-chloro-17b-hydroxy-17a-methyl- § 1300.01 Definitions relating to con- androst-1,4-dien-3-one) trolled substances. (17) desoxymethyltestosterone (17a- (a) Any term not defined in this part methyl-5a-androst-2-en-17b-ol) shall have the definition set forth in (a.k.a. ‘madol‘) section 102 of the Act (21 U.S.C. 802), (18) D1-dihydrotestosterone (a.k.a.‘1- except that certain terms used in part testosterone‘) (17b-hydroxy-5a- 1316 of this chapter are defined at the androst-1-en-3-one) beginning of each subpart of that part. -
(Cyp19a1b-GFP) Zebrafish Embryos
Screening Estrogenic Activities of Chemicals or Mixtures In Vivo Using Transgenic (cyp19a1b-GFP) Zebrafish Embryos Franc¸ois Brion1, Yann Le Page2, Benjamin Piccini1, Olivier Cardoso1, Sok-Keng Tong3, Bon-chu Chung3, Olivier Kah2* 1 Unite´ d’Ecotoxicologie in vitro et in vivo, Direction des Risques Chroniques, Institut National de l’Environnement Industriel et des Risques (INERIS), Verneuil-en- Halatte, France, 2 Universite´ de Rennes 1, Institut de Recherche Sante´ Environnement & Travail (IRSET), INSERM U1085, BIOSIT, Campus de Beaulieu, Rennes France, 3 Taiwan Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan Abstract The tg(cyp19a1b-GFP) transgenic zebrafish expresses GFP (green fluorescent protein) under the control of the cyp19a1b gene, encoding brain aromatase. This gene has two major characteristics: (i) it is only expressed in radial glial progenitors in the brain of fish and (ii) it is exquisitely sensitive to estrogens. Based on these properties, we demonstrate that natural or synthetic hormones (alone or in binary mixture), including androgens or progestagens, and industrial chemicals induce a concentration-dependent GFP expression in radial glial progenitors. As GFP expression can be quantified by in vivo imaging, this model presents a very powerful tool to screen and characterize compounds potentially acting as estrogen mimics either directly or after metabolization by the zebrafish embryo. This study also shows that radial glial cells that act as stem cells are direct targets for a large panel of endocrine disruptors, calling for more attention regarding the impact of environmental estrogens and/or certain pharmaceuticals on brain development. Altogether these data identify this in vivo bioassay as an interesting alternative to detect estrogen mimics in hazard and risk assessment perspective. -
Us Anti-Doping Agency
2019U.S. ANTI-DOPING AGENCY WALLET CARDEXAMPLES OF PROHIBITED AND PERMITTED SUBSTANCES AND METHODS Effective Jan. 1 – Dec. 31, 2019 CATEGORIES OF SUBSTANCES PROHIBITED AT ALL TIMES (IN AND OUT-OF-COMPETITION) • Non-Approved Substances: investigational drugs and pharmaceuticals with no approval by a governmental regulatory health authority for human therapeutic use. • Anabolic Agents: androstenediol, androstenedione, bolasterone, boldenone, clenbuterol, danazol, desoxymethyltestosterone (madol), dehydrochlormethyltestosterone (DHCMT), Prasterone (dehydroepiandrosterone, DHEA , Intrarosa) and its prohormones, drostanolone, epitestosterone, methasterone, methyl-1-testosterone, methyltestosterone (Covaryx, EEMT, Est Estrogens-methyltest DS, Methitest), nandrolone, oxandrolone, prostanozol, Selective Androgen Receptor Modulators (enobosarm, (ostarine, MK-2866), andarine, LGD-4033, RAD-140). stanozolol, testosterone and its metabolites or isomers (Androgel), THG, tibolone, trenbolone, zeranol, zilpaterol, and similar substances. • Beta-2 Agonists: All selective and non-selective beta-2 agonists, including all optical isomers, are prohibited. Most inhaled beta-2 agonists are prohibited, including arformoterol (Brovana), fenoterol, higenamine (norcoclaurine, Tinospora crispa), indacaterol (Arcapta), levalbuterol (Xopenex), metaproternol (Alupent), orciprenaline, olodaterol (Striverdi), pirbuterol (Maxair), terbutaline (Brethaire), vilanterol (Breo). The only exceptions are albuterol, formoterol, and salmeterol by a metered-dose inhaler when used -
A10 Anabolic Steroids Hardcore Info
CONTENTS GENERAL INFORMATION 3 Anabolic steroids – What are they? 4 How do they Work? – Aromatisation 5 More molecules – More problems 6 The side effects of anabolic steroids 7 Women and anabolic steroids 8 Injecting steroids 9 Abscesses – Needle Exchanges 10 Intramuscular injection 11 Injection sites 12 Oral steroids – Cycles – Stacking 13 Diet 14 Where do steroids come from? Spotting a counterfeit 15 Drug Information – Drug dosage STEROIDS 16 Anadrol – Andriol 17 Anavar – Deca-Durabolin 18 Dynabolon – Durabolin – Dianabol 19 Esiclene – Equipoise 20 Primobolan Depot – Proviron – Primobolan orals – Pronobol 21 Sustanon – Stromba, Strombaject – Testosterone Cypionate Testosterone Enanthate 22 Testosterone Propionate – Testosterone Suspension 23 Trenbolone Acetate – Winstrol OTHER DRUGS 24 Aldactone – Arimidex 25 Clenbuterol – Cytomel 26 Ephedrine Hydrochloride – GHB 27 Growth Hormone 28 Insulin 30 Insulin-Like Growth Factor-1 – Human Chorionic Gonadotrophin 31 Tamoxifen – Nubain – Recreational Drugs 32 Steroids and the Law 34 Glossary ANABOLIC STEROIDS People use anabolic steroids for various reasons, some use them to build muscle for their job, others just want to look good and some use them to help them in sport or body building. Whatever the reason, care needs to be taken so that as little harm is done to the body as possible because despite having muscle building effects they also have serious side effects especially when used incorrectly. WHAT ARE THEY? Anabolic steroids are man made versions of the hormone testosterone. Testosterone is the chemical in men responsible for facial hair, deepening of the voice and sex organ development, basically the masculine things Steroids are in a man. used in medicine to treat anaemia, muscle weakness after These masculine effects surgery etc, vascular are called the androgenic disorders and effects of testosterone. -
伊域化學藥業(香港)有限公司 Cyclopentyl Substituted Compounds
® 伊 域 化 學(香 藥 香 港) 業 港有 有 限 限 公 公 司 司 YICK-VIC CHEMICALS & PHARMACEUTICALS (HK) LTD Rm 1006, 10/F, Hewlett Centre, Tel: (852) 25412772 (4 lines) No. 52-54, Hoi Yuen Road, Fax: (852) 25423444 / 25420530 / 21912858 Kwun Tong, E-mail: [email protected] YICKYICK----VICVICVICVIC 伊域伊域伊域 Kowloon, Hong Kong. Site: http://www.yickvic.com Cyclopentyl Substituted Compounds Product Code CAS Product Name CC-0052BA 39746-00-4 (-)-COREY LACTONE BENZOATE CC-3702AD 1211-29-6 (-)-METHYL JASMONATE SPI-4467C 87269-86-1 (-)-OCTAHYDROCYCLOPENTA[B]PYRROLE-2-CARBOXYLIC ACID HYDROCHLORIDE SPI-4467A 93779-30-7 (+/-)-OCTAHYDROCYCLOPENTA[B]PYRROLE-2-CARBOXYLIC ACID HYDROCHLORIDE SPI-2956FG 142217-81-0 (1S,3R,4S)-2-AMINO-9-(4-BENZYLOXY)-3-(BENZYLOXYMETHYL)-2-METHYLIDENECYCLOPENTYL-3H-PURINE-9-ONE SPI-2956FJ 142217-78-5 (2R,3S,5S)-3-(BENZYLOXY)-5-[2-[[(4-METHOXYPHENYL)DIPHENYLMETHYL]AMINO]-6-(PHENYLMETHOXY)-9H-PURIN-9-Y L]-2-(BENZYLOXYMETHYL)CYCLOPENTANOL SPI-1560DH 4167-77-5 1,1-CYCLOPENTANEDICARBOXYLIC ACID DIETHYL ESTER SPI-2808C 5763-44-0 1,2-CYCLOPENTANE DICARBOXIMIDE CC-1957B 1222-05-5 1,3,4,6,7,8-HEXAHYDRO-4,6,6,7,8,8-HEXAMETHYLCYCLOPENTA[G]-2-BENZOPYRAN SPI-1246B 3859-41-4 1,3-CYCLOPENTANEDIONE SPI-1955AA 646-06-0 1,3-DIOXOLANE SPI-3082AA 54078-29-4 1,8-DIAZAFLUOREN-9-ONE UNIE-13864 564-35-2 11-KETOTESTOSTERONE SPI-0098CA 640-87-9 17-ALPHA,21-DIHYDROXYPREGN-4-ENE-3,20-DIONE 21-ACETATE UNIE-13126 302-76-1 17ALPHA-METHYL-17BETA-ESTRADIOL UNIE-14695 3563-27-7 17BETA-DIHYDROQUILIN UNIE-2836 10316-79-7 1-AMINO-1-CYCLOPENTANEMETHANOL SPI-3077CB 61379-64-4 1-AMINO-4-CYCLOPENTYLPIPERAZINE -
University Microfilms, Inc., Ann Arbor, Michigan ADRENOCORTICAL STEROID PROFILE IN
This dissertation has been Mic 61-2820 microfilmed exactly as received BESCH, Paige Keith. ADRENOCORTICAL STEROID PROFILE IN THE HYPERTENSIVE DOG. The Ohio State University, Ph.D., 1961 Chemistry, biological University Microfilms, Inc., Ann Arbor, Michigan ADRENOCORTICAL STEROID PROFILE IN THE HYPERTENSIVE DOG DISSERTATION Presented in Partial Fulfillment of the Requirements for the Degree Doctor of Philosophy in the Graduate School of the Ohio State University By Paige Keith Besch, B. S., M. S. The Ohio State University 1961 Approved by Katharine A. Brownell Department of Physiology DEDICATION This work is dedicated to my wife, Dr. Norma F. Besch. After having completed her graduate training, she was once again subjected to almost social isolation by the number of hours I spent away from home. It is with sincerest appreciation for her continual encouragement that I dedi cate this to her. ACKNOWLEDGMENTS I wish to acknowledge the assistance and encourage ment of my Professor, Doctor Katharine A. Brownell. Equally important to the development of this project are the experience and information obtained through the association with Doctor Frank A. Hartman, who over the years has, along with Doctor Brownell, devoted his life to the development of many of the techniques used in this study. It is also with extreme sincerity that I wish to ac knowledge the assistance of Mr. David J. Watson. He has never complained when asked to work long hours at night or weekends. Our association has been a fruitful one. I also wish to acknowledge the encouragement of my former Professor, employer and good friend, Doctor Joseph W. -
The Metabolism of Desmosterol in Human Subjects During Triparanol Administration
THE METABOLISM OF DESMOSTEROL IN HUMAN SUBJECTS DURING TRIPARANOL ADMINISTRATION DeWitt S. Goodman, … , Joel Avigan, Hildegard Wilson J Clin Invest. 1962;41(5):962-971. https://doi.org/10.1172/JCI104575. Research Article Find the latest version: https://jci.me/104575/pdf Journal of Clinical Investigation Vol. 41, No. 5, 1962 THE METABOLISM OF DESMOSTEROL IN HUMAN SUBJECTS DURING TRIPARANOL ADMINISTRATION * BY DEWITT S. GOODMAN, JOEL AVIGAN AND HILDEGARD WILSON (From the Section on Metabolism, National Heart Institute, and the National Institute of Arthritis and Metabolic Diseases, Bethesda, Md.) (Submitted for publication October 25, 1961; accepted January 25, 1962) Recent studies with triparanol (1-[p-,3-diethyl- Patient G.B. was a 55 year old man with known arterio- aminoethoxyphenyl ]-1- (p-tolyl) -2- (p-chloro- sclerotic heart disease and mild hypercholesterolemia; phenyl)ethanol) have demonstrated that this com- since 1957 he had maintained a satisfactory and stable cardiac status. At the time of the present study he had pound inhibits cholesterol biosynthesis by blocking been taking 250 mg triparanol daily for 4 weeks, and had the reduction of 24-dehydrocholesterol (desmos- a total serum sterol level in the high normal range. terol) to cholesterol (2-4). Administration of Patient F.A. was a 40 year old man with a 4- to 5-year triparanol to laboratory animals and to man re- history of gout and essential hyperlipemia. At the time sults in of of this study he had been on an isocaloric low purine diet the accumulation desmosterol in the for several weeks, and both the gout and hyperlipemia plasma and tissues, usually with some concom- were in remission. -
Effects of Performance Enhancing Drugs on the Health of Athletes and Athletic Competition
S. HRG. 106–1074 EFFECTS OF PERFORMANCE ENHANCING DRUGS ON THE HEALTH OF ATHLETES AND ATHLETIC COMPETITION HEARING BEFORE THE COMMITTEE ON COMMERCE, SCIENCE, AND TRANSPORTATION UNITED STATES SENATE ONE HUNDRED SIXTH CONGRESS FIRST SESSION OCTOBER 20, 1999 Printed for the use of the Committee on Commerce, Science, and Transportation ( U.S. GOVERNMENT PRINTING OFFICE 75–594 PDF WASHINGTON : 2002 For sale by the Superintendent of Documents, U.S. Government Printing Office Internet: bookstore.gpo.gov Phone: toll free (866) 512–1800; DC area (202) 512–1800 Fax: (202) 512–2250 Mail: Stop SSOP, Washington, DC 20402–0001 VerDate 11-MAY-2000 10:45 Jun 07, 2002 Jkt 075594 PO 00000 Frm 00001 Fmt 5011 Sfmt 5011 75594.TXT SCOM1 PsN: SCOM1 SENATE COMMITTEE ON COMMERCE, SCIENCE, AND TRANSPORTATION ONE HUNDRED SIXTH CONGRESS FIRST SESSION JOHN MCCAIN, Arizona, Chairman TED STEVENS, Alaska ERNEST F. HOLLINGS, South Carolina CONRAD BURNS, Montana DANIEL K. INOUYE, Hawaii SLADE GORTON, Washington JOHN D. ROCKEFELLER IV, West Virginia TRENT LOTT, Mississippi JOHN F. KERRY, Massachusetts KAY BAILEY HUTCHISON, Texas JOHN B. BREAUX, Louisiana OLYMPIA J. SNOWE, Maine RICHARD H. BRYAN, Nevada JOHN ASHCROFT, Missouri BYRON L. DORGAN, North Dakota BILL FRIST, Tennessee RON WYDEN, Oregon SPENCER ABRAHAM, Michigan MAX CLELAND, Georgia SAM BROWNBACK, Kansas MARK BUSE, Staff Director MARTHA P. ALLBRIGHT, General Counsel IVAN A. SCHLAGER, Democratic Chief Counsel and Staff Director KEVIN D. KAYES, Democratic General Counsel (II) VerDate 11-MAY-2000 10:45 Jun 07, 2002 Jkt 075594 PO 00000 Frm 00002 Fmt 5904 Sfmt 5904 75594.TXT SCOM1 PsN: SCOM1 C O N T E N T S Page Hearing held October 20, 1999 .............................................................................. -
Performance-Enhancing Drugs in Sports: How Chemists Catch Users
In the Classroom Performance-Enhancing Drugs in Sports: How Chemists Catch Users T. C. Werner* Chemistry Department, Union College, Schenectady, New York 12308, United States *[email protected] Caroline K. Hatton Sports Anti-Doping Science Consultant, P.O. Box 4795, Culver City, California 90231-4795, United States What do the following prominent athletes have in com- Olympic Analytical Laboratory and Anti-Doping Research, Inc. mon: Floyd Landis, Mark McGwire, Marion Jones, Ben Johnson, A description of the course can be found on the Web (3). and Lyle Alzado? All have been suspected of using performance- enhancing drugs (PEDs) in recent decades and have eventually Stimulants confessed. Over this period, major doping investigations have included the BALCO (Bay Area Laboratory Co-Operative) case, Athletes have used stimulants, such as caffeine, strychnine, which involved doping in various sports instigated by a company and cocaine since the 19th century (4). The 2010 WADA list in the San Francisco area, and the Mitchell Report, which prohibits all stimulants in competition and names about describes PED use (particularly steroids) in Major League Base- 60 examples. The most notorious is amphetamine (Figure 1), ball (MLB). Moreover, the Tour de France has been wracked by which stimulates the central nervous system by releasing doping allegations for decades, causing teams to lose sponsors neurotransmitters, such as dopamine, into the synapse between recently and struggle to maintain fan support, even in cycling- neurons. As a result, amphetamine combats fatigue (5).Stimu- crazy Europe. All of the sports headlines generated by these lantabuseputsathletesatriskforheartrhythmanomaliesand incidents have a chemical “back story” that is rich in relevant for injury to themselves and their competitors, among other examples for undergraduate chemistry and biochemistry courses, things. -
Type 1 Diabetes
Type 1 Diabetes Clinical Management of the Athlete Ian Gallen Editor Type 1 Diabetes Clinical Management of the Athlete Editor Ian Gallen Diabetes Centre Wycombe Hospital High Wycombe UK ISBN 978-0-85729-753-2 e-ISBN 978-0-85729-754-9 DOI 10.1007/978-0-85729-754-9 Springer London Dordrecht Heidelberg New York British Library Cataloguing in Publication Data A catalogue record for this book is available from the British Library Library of Congress Control Number: 2012934821 © Springer-Verlag London Limited 2012 Apart from any fair dealing for the purposes of research or private study, or criticism or review, as permitted under the Copyright, Designs and Patents Act 1988, this publication may only be reproduced, stored or transmitted, in any form or by any means, with the prior permission in writing of the publishers, or in the case of reprographic reproduction in accordance with the terms of licenses issued by the Copyright Licensing Agency. Enquiries concerning reproduction outside those terms should be sent to the publishers. The use of registered names, trademarks, etc., in this publication does not imply, even in the absence of a specifi c statement, that such names are exempt from the relevant laws and regulations and therefore free for general use. Product liability: The publisher can give no guarantee for information about drug dosage and application thereof contained in this book. In every individual case the respective user must check its accuracy by consulting other pharmaceutical literature. Printed on acid-free paper Springer is part of Springer Science+Business Media (www.springer.com) This book is dedicated to my parents Louis and Barbara for their lifelong love, encouragement and support, to my wife Susan for our happy life together and to our children Robert and Hannah who make my life meaningful.