RESEARCH ARTICLE DNA barcoding of odonates from the Upper Plata basin: Database creation and genetic diversity estimation Ricardo Koroiva1,2*, Mateus Pepinelli3,4, Marciel Elio Rodrigues5, Fabio de Oliveira Roque2, Aline Pedroso Lorenz-Lemke6, Sebastian Kvist3,4 1 Ecology and Conservation Graduate Program, Universidade Federal de Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil, 2 LaboratoÂrio de Ecologia, Universidade Federal de Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil, 3 Department of Natural History, Royal Ontario Museum, a1111111111 Toronto, Ontario, Canada, 4 Department of Ecology and Evolutionary Biology, University of Toronto, Toronto, a1111111111 Ontario, Canada, 5 LaboratoÂrio de Organismos AquaÂticos, Universidade Estadual de Santa Cruz, IlheÂus, a1111111111 Bahia, Brazil, 6 LaboratoÂrio de EvolucËão e Biodiversidade, Universidade Federal de Mato Grosso do Sul, a1111111111 Campo Grande, Mato Grosso do Sul, Brazil a1111111111 *
[email protected] Abstract OPEN ACCESS We present a DNA barcoding study of Neotropical odonates from the Upper Plata basin, Citation: Koroiva R, Pepinelli M, Rodrigues ME, Roque FdO, Lorenz-Lemke AP, Kvist S (2017) DNA Brazil. A total of 38 species were collected in a transition region of ªCerradoº and Atlantic barcoding of odonates from the Upper Plata basin: Forest, both regarded as biological hotspots, and 130 cytochrome c oxidase subunit I (COI) Database creation and genetic diversity estimation. barcodes were generated for the collected specimens. The distinct gap between intraspe- PLoS ONE 12(8): e0182283. https://doi.org/ 10.1371/journal.pone.0182283 cific (0±2%) and interspecific variation (15% and above) in COI, and resulting separation of Barcode Index Numbers (BIN), allowed for successful identification of specimens in 94% of Editor: Sebastian D.