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I HIGH MASS ACCURACY COUPLED to SPATIALLY-DIRECTED
HIGH MASS ACCURACY COUPLED TO SPATIALLY-DIRECTED PROTEOMICS FOR IMPROVED PROTEIN IDENTIFICATIONS IN IMAGING MASS SPECTROMETRY EXPERIMENTS By David Geoffrey Rizzo Dissertation Submitted to the Faculty of the Graduate School of Vanderbilt University in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY in Chemistry August, 2016 Nashville, Tennessee Approved: Richard M. Caprioli, Ph.D. Kevin L. Schey, Ph.D. John A. McLean, Ph.D. Michael P. Stone, Ph.D. i Copyright © 2016 by David Geoffrey Rizzo All Rights Reserved ii This work is dedicated to my family and friends, who have shown nothing but support for me in all of life’s endeavors. iii ACKNOWLEDGEMENTS “As we express our gratitude, we must never forget that the highest appreciation is not to utter words, but to live by them.” - John F. Kennedy – There are many people I must thank for showing kindness, encouragement, and support for me during my tenure as a graduate student. First and foremost, I would like to thank my research advisor, Richard Caprioli, for providing both ample resources and guidance that allowed me to grow as a scientist. Our discussions about my research and science in general have helped me become a much more focused and discerning analytical chemist. I must also thank my Ph.D. committee members, Drs. Kevin Schey, John McLean, and Michael Stone, who have brought valuable insight into my research and provided direction along the way. My undergraduate advisor, Dr. Facundo Fernández, encouraged me to begin research in his lab and introduced me to the world of mass spectrometry. -
An Ethnography of the Spring Festival
IMAGINING CHINA IN THE ERA OF GLOBAL CONSUMERISM AND LOCAL CONSCIOUSNESS: MEDIA, MOBILITY, AND THE SPRING FESTIVAL A dissertation presented to the faculty of the College of Communication of Ohio University In partial fulfillment of the requirements for the degree Doctor of Philosophy Li Ren June 2003 This dissertation entitled IMAGINING CHINA IN THE ERA OF GLOBAL CONSUMERISM AND LOCAL CONSCIOUSNESS: MEDIA, MOBILITY AND THE SPRING FESTIVAL BY LI REN has been approved by the School of Interpersonal Communication and the College of Communication by Arvind Singhal Professor of Interpersonal Communication Timothy A. Simpson Professor of Interpersonal Communication Kathy Krendl Dean, College of Communication REN, LI. Ph.D. June 2003. Interpersonal Communication Imagining China in the Era of Global Consumerism and Local Consciousness: Media, Mobility, and the Spring Festival. (260 pp.) Co-directors of Dissertation: Arvind Singhal and Timothy A. Simpson Using the Spring Festival (the Chinese New Year) as a springboard for fieldwork and discussion, this dissertation explores the rise of electronic media and mobility in contemporary China and their effect on modern Chinese subjectivity, especially, the collective imagination of Chinese people. Informed by cultural studies and ethnographic methods, this research project consisted of 14 in-depth interviews with residents in Chengdu, China, ethnographic participatory observation of local festival activities, and analysis of media events, artifacts, documents, and online communication. The dissertation argues that “cultural China,” an officially-endorsed concept that has transformed a national entity into a borderless cultural entity, is the most conspicuous and powerful public imagery produced and circulated during the 2001 Spring Festival. As a work of collective imagination, cultural China creates a complex and contested space in which the Chinese Party-state, the global consumer culture, and individuals and local communities seek to gain their own ground with various strategies and tactics. -
3-4 Booklist by Title - Full
3-4 Booklist by Title - Full When using this booklist, please be aware of the need for guidance to ensure students select texts considered appropriate for their age, interest and maturity levels. PRC Title/Author Publisher Year ISBN Annotations 9404 100 Australian poems for children Random House 2002 9781740517751 From emus to magic puddings, this feast of Australian poems for Griffith, Kathryn & Scott-Mitchell, Claire (eds)Australia Pty Ltd children is fresh and familiar. Written by grown-ups and kids, with & Rogers, Gregory (ill) beautiful illustrations, it reveals what is special about growing up in Australia. 660416 100 ways to fly University of 2019 9780702262500 In 100 Ways to Fly you'll find a poem for every mood - poems to make Taylor, Michelle Queensland Press you laugh, feel silly or to twist your tongue, to make you courageous enough for a new adventure or to help you soar. 537 27th annual African hippopotamus race Puffin Australia 1985 9780140309911 Go behind the scenes as eight year old Edward, the hippopotamus, Lurie, Morris trains for the greatest swimming marathon of all. 18410 4F for freaks Allen & Unwin Pty Ltd 2006 9781741140910 4F pride themselves on frightening teachers out of the classroom. It Hobbs, Leigh seems that they have met their match with Miss Corker who has a few tricks of her own up her sleeves. 9581 500 hats of Bartholomew Cubbins, The Random House 1989 9780394844848 Bartholomew is selling cranberries when the king passes by. A big cry is Seuss, Dr Australia Pty Ltd heard, 'Hats off to the King', which Bartholomew does but he still has a hat on his head. -
Curriculum Vitae LINGJUN LI
Curriculum Vitae LINGJUN LI University of Wisconsin-Madison School of Pharmacy & Department of Chemistry 777 Highland Avenue Madison, WI 53705-2222 E-mail: [email protected] Phone : (608)265-8491 Fax : (608)262-5345 EDUCATION Ph.D. University of Illinois at Urbana-Champaign, 1995-2000 May 2000 Chemistry major (analytical and biomolecular) B.E. Beijing University of Technology, Beijing, China, 1987-1992 July 1992 Chemistry major (environmental analytical chemistry) EXPERTISE AND RESEARCH INTERESTS Bioanalytical chemistry, neurochemistry, biological mass spectrometry, neuropeptides, proteomics, peptidomics Research in my laboratory is focused on developing and implementing an array of novel mass spectrometry (MS) based methodologies to answer questions about the most complex and elusive set of signaling molecules, the neuropeptides, and gain new insights into the roles of peptide hormones and neurotransmitters play in the plasticity of neural circuits and behavior. Emphasis has been placed on constructing a multi-faceted and integrated platform that include high resolution in-situ peptide mapping, high sensitivity micro-separation techniques coupled with tandem MS de novo sequencing, isotopic labeling strategies, and new bioinformatics tools to allow large-scale discovery and functional analysis of novel neuropeptides. Furthermore, both mass spectrometric imaging technologies and in vivo microdialysis sampling tools have been implemented to follow neuropeptide distribution and secretion in unprecedented details. Towards the goal of -
2017 WEEKLY BULLETIN DEPARTMENT of CHEMISTRY, NORTHWESTERN UNIVERSITY EVANSTON, ILLINOIS April 24, 2017
2017 WEEKLY BULLETIN DEPARTMENT OF CHEMISTRY, NORTHWESTERN UNIVERSITY EVANSTON, ILLINOIS April 24, 2017 For full schedule, including Center events, please see the Department Calendar: http://www.chemistry.northwestern.edu/events/calendar.html Tuesday April 25th: Faculty Lunch Seminar: Neil Kelleher Tech K140 12:00 – 1:00pm Friday April 28th: Chemistry Department Colloquium: Stacey F. Bent, Stanford University Tech LR3 4:00-5:00pm BIP BIP meets every Friday 10-11:00am in Tech K140 Arrivals We did not have any new arrivals Announcements 10th Annual ANSER Solar Energy Symposium April 27-28, 2017: The Argonne-Northwestern Solar Energy Research Center (ANSER) and the Institute for Sustainability and Energy at Northwestern (ISEN) are delighted to host the 10th annual ANSER Solar Energy Symposium – “Solar Electricity.” As our understanding of the impact of climate change continues to grow, so too does the global trend towards a clean-energy economy. The last two years have seen organic photovoltaics reach efficiencies of 11.5 percent, quantum dot solar cells reach efficiencies of 11.3 percent and perovskite solar cells continue their meteoric rise to efficiencies of 22.1 percent, paving the way for continually decreasing photovoltaic costs. This encouraging march toward a cleaner power sector cannot be ignored, and is built on the foundation of innovative research being carried out at collaborative scientific hubs such as the ANSER Center. The thematic focus of this year’s Symposium is “Solar Electricity,” and we are honored to host a star-studded lineup of speakers. These photovoltaic leaders will present life-cycle analyses, report the current state-of- the-art, outline challenges ahead, and propose new ideas to pursue in this rapidly growing field of solar photovoltaic research. -
25 Years of the Swiss Chemical Society's Division of Analytical
Columns CHIMIA 2017, 71, No. 12 861 doi:10.2533/chimia.2017.861 Chimia 71 (2017) 861 © Swiss Chemical Society Division of Analytical Sciences A Division of the Swiss Chemical Society 1992–2017: 25 Years of the Swiss Chemical Society’s the fact that its scope is not limited to chemistry but also includes Division of Analytical Sciences – Past, Present and physical techniques and biological methods. Future Activities Walter Giger*, Fritz Erni, and Ernst Halder Membership – Organisation – Communication *Correspondence: Prof. W. Giger, CH-8049 Zurich, E-mail: [email protected] In 1996, almost 300 members of the Swiss Chemical Society were also members of the DAC. By 2017, membership had Keywords: Analytical Sciences · Division of Analytical Sciences increased to 585 (22% of Swiss Chemical Society members). Most of the DAC’s activities were managed and organised by Launch in the 1990s – Scope and Goals – Name therelativelylargeDAC Board,comprisingabout9to17members, several of whom were active for many years. Every other year, In the early 1990s, the chemists’ professional societies in the DAC Board held a retreat, where current endeavours were Switzerland were reorganised. The major event was the merger evaluated and future projects thoroughly discussed and planned. of the Swiss Chemical Society and the Association of Swiss In 1999, the importance of the internet for gaining visibility Chemists in spring 1992. Around the same time, the analytical and meeting members’ needs became evident. Ernst Halder chemists informally organised in the Comité Suisse de Chimie and Käthi Halder initiated and maintained a divisional website Analytique (see CHIMIA 1990, 44(9), 298–299) became the at www.sach.ch, including, most importantly, information on Analytical Chemistry Section (SACh) of the New Swiss Chemical the training programme. -
Developing Next Generation Technologies for Spatially Targeted
Developing Next Generation Technologies for Spatially Targeted Proteomics By Daniel Joseph Ryan Dissertation Submitted to the Faculty of the Graduate School of Vanderbilt University in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY in Chemistry June 30th, 2019 Nashville, Tennessee Approved: Richard M. Caprioli, Ph.D. Jeffrey M. Spraggins, Ph.D. John A. McLean, Ph.D. Lars Plate, Ph.D. Kevin L. Schey, Ph.D. Copyright © 2019 by Daniel Joseph Ryan All Rights Reserved ii ACKNOWLEDGEMENTS It is with the help of many people that I am afforded the unique privilege of being able to sit here and write out an acknowledgements section for my dissertation. First and foremost, I would like to thank both of advisors, Dr. Richard Caprioli and Dr. Jeff Spraggins. Richard, you have pushed me both scientifically and personally. You have led by example and I am very grateful to have had the opportunity to spend my graduate career in your laboratory, it is not something I take for granted. Jeff, you helped me gain traction upon entering the lab, gave me direction, and have been an integral part of my journey while at Vanderbilt. You went above and beyond what is expected of any advisor to help mold me into the scientist I am today, and I am grateful to call you a mentor and more importantly, a friend. To my entire committee, Kevin Schey, John McLean, and Lars Plate; I am forever thankful for the time you have taken to help push me towards excellence throughout this journey. I want to thank my lab mates, who are also my closest friends, for their support and friendship throughout this period of my life. -
Foundations of Biomedical Sciences Module IV
Foundations in Biomedical Sciences IV: Mechanisms of Cell Communication Spring 2012 Tuesdays, Thursdays, and Fridays, 9:30 am to 11:20 am Fridays, 12:15 pm to 1:45 pm Course description The fourth module of the Foundations in Biomedical Sciences course will focus on the mechanisms of cell communication. This module will begin by discussing overarching concepts before examining the specific types of molecules that initiate and transduce signals. Examples of cell signaling and subsequent cellular responses will then be considered in different contexts to provide a framework on which future learning can be applied. As the module progresses, the complexity of the systems explored will increase from individual cells to multicellular environments such as tissues, organs and organisms. In addition, normal processes as well as the dysregulation of cell-cell communication in disease will be studied. The course will be aimed towards first year Ph.D. students in the Division of Graduate Medical Sciences. The class will be taught by members of the Division in a variety of Departments utilizing a combination of traditional lectures and discussion sections focusing on primary research. There will be a total of 7 hours of class time per week. Supplementary study materials will be made available using Blackboard (www.blackboard.bu.edu) to aid students in the review of the material. Reading materials will be taken primarily from the scientific literature and will utilize examples of classical studies as well as recent works. Students will be evaluated on their performance on a quiz, problem set, and examination along with active participation in discussion sections. -
20Th International Mass Spectrometry Conference
IMSC 2014 20th International Mass Spectrometry Conference August 24-29, 2014 Geneva, Switzerland PROGRAM v. 17.09.2014 More targets. More accurately. Faster than ever. Analytical challenges grow in quantity and complexity. Quantify a larger number of compounds and more complex analytes faster and more accurately with our new portfolio of LC-MS instruments, sample prep solutions and software. High-resolution, accurate mass solutions using Thermo Scientific™ Orbitrap™ MS quantifies all detectable compounds with high specificity, and triple quadrupole MS delivers SRM sensitivity and speed to detect targeted compounds more quickly. Join us in meeting today’s challenges. Together we’ll transform quantitative science. Quantitation transformed. • Discover more at thermoscientific.com/quan-transformed • Visit thermoscientific.com/imsc or booth 23 for more information © 2014 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are © 2014 Thermo Fisher Scientific Inc. All rights the property of Thermo Fisher Scientific and its subsidiaries. the property Thermo Scientific™ Q Exactive™ HF MS Thermo Scientific™ TSQ Quantiva™ MS Thermo Scientific™ TSQ Endura™ MS Screen and quantify known and unknown targets Leading SRM sensitivity and speed Ultimate SRM quantitative value and with HRAM Orbitrap technology in a triple quadrupole MS/MS unprecedented usability TABLE OF CONTENTS th 1. Welcome from the Chairs of the 20 IMSC ............................................................................................................................ -
2Nd ANNUAL NORTH AMERICAN MASS SPECTROMETRY SUMMER SCHOOL
2nd ANNUAL NORTH AMERICAN MASS SPECTROMETRY SUMMER SCHOOL JULY 21-24, 2019 | MADISON, WISCONSIN Parabola of Neon (1913) Featured on the cover is an early 20th century parabola mass spectrograph. The early mass spectrometers, pioneered by J. J. Thomson, used electric and magnetic fields to disperse ion populations on photographic plates. Depending on their masses, the ions were dispersed along parabolic lines with those of the highest energy landing in the center and those with the least extending to the outermost edges. Positive ions are imaged on the upper half of the parabola while negative ions are deflected to the bottom half. Note that Ne produces two lines in the spectrum. Francis Aston, a former Thomson student, concluded from these data that stable elements also must have isotopes. These observations won Aston the Nobel Prize in Chemistry in 1922. Grayson, M.A. Measuring Mass: From Positive Rays to Proteins. 2002. Chemical Heritage Press, Philadelphia. Welcome to the 2nd Annual North American Mass Spectrometry Summer School We are proud to assemble world-leading experts in mass spectrometry for this second annual mass spectrometry summer school. We aim for you to experience an engaging and inspiring program covering the fundamentals of mass spectrometry and how to apply this tool to study biology. Also infused in the course are several workshops aimed to promote professional development. We encourage you to actively engage in discussion during all lectures, workshops, and events. This summer school is made possible through generous funding from the National Science Foundation (Plant Genome Research Program, Grant No. 1546742), the National Institutes of Health National Center for Quantitative Biology of Complex Systems (P41 GM108538), and the Morgridge Institute for Research. -
Nature Milestones Mass Spectrometry October 2015
October 2015 www.nature.com/milestones/mass-spec MILESTONES Mass Spectrometry Produced with support from: Produced by: Nature Methods, Nature, Nature Biotechnology, Nature Chemical Biology and Nature Protocols MILESTONES Mass Spectrometry MILESTONES COLLECTION 4 Timeline 5 Discovering the power of mass-to-charge (1910 ) NATURE METHODS: COMMENTARY 23 Mass spectrometry in high-throughput 6 Development of ionization methods (1929) proteomics: ready for the big time 7 Isotopes and ancient environments (1939) Tommy Nilsson, Matthias Mann, Ruedi Aebersold, John R Yates III, Amos Bairoch & John J M Bergeron 8 When a velocitron meets a reflectron (1946) 8 Spinning ion trajectories (1949) NATURE: REVIEW Fly out of the traps (1953) 9 28 The biological impact of mass-spectrometry- 10 Breaking down problems (1956) based proteomics 10 Amicable separations (1959) Benjamin F. Cravatt, Gabriel M. Simon & John R. Yates III 11 Solving the primary structure of peptides (1959) 12 A technique to carry a torch for (1961) NATURE: REVIEW 12 The pixelation of mass spectrometry (1962) 38 Metabolic phenotyping in clinical and surgical 13 Conquering carbohydrate complexity (1963) environments Jeremy K. Nicholson, Elaine Holmes, 14 Forming fragments (1966) James M. Kinross, Ara W. Darzi, Zoltan Takats & 14 Seeing the full picture of metabolism (1966) John C. Lindon 15 Electrospray makes molecular elephants fly (1968) 16 Signatures of disease (1975) 16 Reduce complexity by choosing your reactions (1978) 17 Enter the matrix (1985) 18 Dynamic protein structures (1991) 19 Protein discovery goes global (1993) 20 In pursuit of PTMs (1995) 21 Putting the pieces together (1999) CITING THE MILESTONES CONTRIBUTING JOURNALS UK/Europe/ROW (excluding Japan): The Nature Milestones: Mass Spectroscopy supplement has been published as Nature Methods, Nature, Nature Biotechnology, Nature Publishing Group, Subscriptions, a joint project between Nature Methods, Nature, Nature Biotechnology, Nature Chemical Biology and Nature Protocols. -
Page No. 1 Science & Technology Ready Reckoner RRP 2020
Science & Technology www.laex.in Page No. 1 https://elearn.laex.in Science & Technology Ready Reckoner RRP 2020 www.laex.in https://elearn.laex.in Science & Technology Ready Reckoner RRP 2020 SCIENCE & TECHNOLOGY INDEX Page No. Sl.No. Topic From To 01. Biotechnology 1 21 02. Vaccines and Diseases 23 34 03. Space Technology 34 65 04. Defense Technology 65 78 05. Emerging Technologies in ICT 78 110 06. Energy Issues in News 112 118 07. Intellectual Property Rights 118 125 08. Advancement in Fundamental Physics 126 134 09. Technologies And Innovations In Day To Day Life 135 145 www.laexias.com https://elearn.laex.in Science & Technology Ready Reckoner RRP 2020 1. Biotechnology Cell Organelles and Functions Cell Oganelle Organelle Functions Introduction to Biotechnology Nucleus Cpmtaoms DNA Nucleolus Makes ribosomes Made up of two words: ‘bio’ and ‘technology’. Mitochondria Releases Energy ‘Bio’ means life and ‘technology’ means Converts sunlight into Chloroplast application or harnessing of science for a sugar specific purpose. Ribosomes Makes protein Therefore, the term ‘biotechnology’ refers to Endoplasmic Carries proteins in Reticulum passage ways modification or use of any living organism for Packages and ships Golgi Bodies any useful purpose. protein The term was coined by KárolyEreky in 1919 Lysosomes Breaks stuff down Protects and supports the Cell Wall Basics: cell Cell Controls what Cell membrane Cells are the basic building blocks of living enters/exists the cell Vacuole Storage things. Fluid that holds Cytoplasm The human body is composed of trillions of organelles cells, all with their own specialised function. Flagella Movement Cells group together to form tissues, which in Pili Attach to other cells Protects bacteria and turn group together to form organs, such as Capsule causes disease the heart and brain.