H3 (K27ac) monoclonal Summary: are basic nuclear antibody that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist Catalog Number: MAB10256 of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four Regulation Status: For research use only (RUO) core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a Product Description: Mouse monoclonal antibody linker histone, H1, with the DNA between the raised against synthetic peptide of Histone H3 (K27ac). nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the Immunogen: A synthetic peptide (conjugated with KLH) histone H3 family. Transcripts from this gene lack polyA corresponding to Histone H3 acetylated at lysine 27. tails; instead, they contain a palindromic termination element. This gene is located separately from the other Host: Mouse H3 that are in the histone gene cluster on 6p22-p21.3. [provided by RefSeq] Reactivity: Human

Applications: ChIP, ELISA, WB (See our web site product page for detailed applications information)

Protocols: See our web site at http://www.abnova.com/support/protocols.asp or product page for detailed protocols

Form: Liquid

Purification: A purification

Isotype: IgG1

Recommend Usage: ELISA (1:3000) Western Blot (1:1000-1:2000) ChIP (1-5 ul/ChIP) The optimal working dilution should be determined by the end user.

Storage Buffer: In PBS (0.05% sodium azide)

Storage Instruction: Store at -20°C. For long term storage store at -80°C. Aliquot to avoid repeated freezing and thawing.

Entrez GeneID: 8290

Gene Symbol: HIST3H3

Gene Alias: H3.4, H3/g, H3FT, H3t, MGC126886, MGC126888

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