Blood Culture Aseptic Technique: Peripheral Draw (Recommend 2 staff members for this procedure)

Step Key Point Reason Gather supplies Saniwipe, 2 face masks, 4 alcohol pads, 2 chloraprep frepps, sterile Use AIDET!! Hello I am ___ and I will be your RN today. I have received gloves, 2 transfer devices, 2 10 mL syringes, 21g butterfly, lab bag, 2 an order from your doctor to draw blood cultures. This test is performed 2x2 gauze, bandaid, tourniquet, lab labels, BC bottles, instructions to see if there is an infection in your blood. The process will take around 1 20-30 minutes to ensure that the test results are as accurate as 2 Identify patient, verify and explain procedure possible. I will draw blood from your arm and from your line (if Hand hygiene and don clean gloves indicated). 3 Palpate vein Palpate vein and choose venipuncture site prior to opening supplies to avoid leaving supplies open while trying to find an adequate site. 4 Apply face mask to self and patient (if patient not on Additional mask needed if patient is not on ventilator or wearing an ventilator or wearing an oxygen mask) oxygen mask. 5 Clean bedside table with germicidal wipe Supplies should be placed on surface that is clean and dry. Decrease contaminates in work area. 6 7 Hand hygiene and don clean gloves Arrange supplies. Ensure all supplies remain sterile and accessible. Obtaining and utilizing specific supplies is evidence based to decrease 8 contaminations. Prep venipuncture site with minimum of 2 chlorapreps, Frepp has a rough surface that helps remove cellular debris. Antimicrobial agent cleans skin so that organisms do not enter puncture scrub back and forth for a minimum of 30 seconds per DO NOT repaplate site! site or contaminate culture. Drying ensures complete antimicrobial prep and allow to completely air dry If patient is allergic to chlorahexadine, clean site with alcohol. action. 9 Apply tourniquet Tourniquet should be 2-4 inches above venipuncture site. Leave the Tourniquet blocks venous return to the heart from extremity causing tourniquet on no longer than 1 minute to decrease the potential for veins to dilate for easier visibiity. 10 hemolysis. Hand hygiene & don sterile gloves Keep sterile glove package available to place syringes of blood on after Aseptic technique is used while drawing blood cultures. The sterile obtaining sample for step 12 gloves are used to promote awareness & keep the site free of 11 contaminants. Perform venipuncture, obtain two 10 mL syringes of blood 10 ml is the optimal volume of blood per blood culture bottle. Do not over 2 person technique aides in reducing potential clot development in the and place on sterile field (sterile glove package) or under fill! This step is helpful to have a second person connect the specimen syringe. specimen syringe to the transfer device and place specimen into culture 12 bottle. 13 Release tourniquet 14 Apply bandage and stop bleeding Clean tops of culture bottles with alcohol wipe, remove If you only have enough blood for one bottle, fill aerobic bottle only. Fill aerobic bottle first, especially if you were only able to collect 10 mL of alcohol wipe and allow to dry. Attach needleless syringe to Most pathogens are aerobic. blood. Do not force blood into culture bottle - this may cause hemolysis. transfer device. Plunge transfer device through bottle. Fill Remove alcohol wipe: DO NOT plunge through alcohol wipe. Less volume increases risk of contamination. each bottle with 8-10 mL of blood. Do not over or under 15 fill. Remove gloves and perform hand hygiene. 16 Label and scan specimen at bedside according to policy The patient label should not cover the bottle lot number or expiration Refer to picture reference. with time, date, location of draw, and Cerner sign in date. Avoid covering the narrow window, on the side of the bottle, this is (AIDET After procedure) Thank you for your time, and thank you for used by lab to visually inspect the specimen. choosing INTEGRIS Health. Please notify me if you have any questions 17 or concerns. Send to Microbiology via central lab. 18 Revised 2/11/16