UBE2H Protein UBE2H Protein

Catalogue # Aliquot Size

U223-30H-20 20 µg U223-30H-50 50 µg

UBE2H Protein Full length recombinant protein expressed in E. coli cells

Catalog # U223-30H

Lot # J581 -2

Product Description Purity

Recombinant full length human UBE2H was expressed in E. coli cells using an N-terminal His tag. The gene accession number is NM_003344 .

The purity of UBE2H was determined Gene Aliases to be >95% by densitometry. Approx. MW 21 kDa . E2-20K; UBC8; UBCH; UBCH2

Formulation

Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF,

0.25mM DTT, 25% glycerol.

Storage and Stability

Store product at –70 oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background

UBE2H or ubiquitin-conjugating enzyme E2H is a member of the E2 ubiquitin-conjugating enzyme family which is located on chromosome 7 (1). UBE2H is known to act on histones and cytoskeletal proteins, both involved in the UBE2H Protein degenerative pathway of the motor neuron. UBE2H Full length recombinant protein expressed in E. coli cells expression is increased during erythroid differentiation of hCD34(+) cells. Tal1 transcription factor, which is essential Catalog Number U223-30H for the development of the hematopoietic system and Specific Lot Number J581-2 plays a role during definitive erythropoiesis in the adult, Purity >95% activates UBE2H expression, whereas Tal1 knock-down Concentration 0.1µg/ µl Stability 1yr At –70 oC from date of shipment reduced UBE2H expression and ubiquitin transfer activity Storage & Shipping Store product at –70 oC. For optimal (2). storage, aliquot target into smaller quantities after centrifugation and References store at recommended temperature. For most favorable performance, 1. Kaiser, P. et.al: Human ubiquitin-conjugating enzyme avoid repeated handling and homologous to yeast UBC8. J. Biol. Chem. 269: 8797-8802, multiple freeze/thaw cycles. Product 1994. shipped on dry ice. 2. Lausen J,Targets of the Tal1 transcription factor in erythrocytes: E2 ubiquitin conjugase regulation by Tal1. J Biol Chem. 2010 Feb 19;285(8):5338-46.

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FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS.