1 Transcriptomic and Proteomic Insight into the Mechanism of Cyclooctasulfur- versus 2 Thiosulfate-Oxidation by the Chemolithoautotroph Sulfurimonas denitrificans 3 4 Florian Götz1,2,#, Petra Pjevac3,#, Stephanie Markert2,5, Jesse McNichol1,6, Dörte Becher7, 5 Thomas Schweder2,5, Marc Mussmann3,4, Stefan M. Sievert1,* 6 7 1Biology Department, Woods Hole Oceanographic Institution, Woods Hole, MA 02543, USA 8 2Department of Pharmaceutical Biotechnology, Institute of Pharmacy, University of Greifswald, 9 D-17487 Greifswald, Germany 10 3Division of Microbial Ecology, Department of Microbiology and Ecosystem Science, Research 11 Network ‘Chemistry Meets Microbiology’, University of Vienna, Althanstr- 14, 1090, Vienna, 12 Austria 13 4Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Celsiusstr. 1, 14 28359 Bremen, Germany 15 5Institute of Marine Biotechnology, Walther-Rathenau-Strasse 49, D-17489 Greifswald, 16 Germany. 17 6Present Address: Department of Biological Sciences, University of Southern California, Los 18 Angeles, CA 90089 19 7Institute of Microbiology, University of Greifswald, D-17487 Greifswald, Germany 20 #Contributed equally to this work 21 *corresponding author; email:
[email protected] 22 23 Running title: S8 metabolism in Sulfurimonas denitrificans 1 24 Originality-Significance Statement: To the best of our knowledge, this is the first proteomic 25 and transcriptomic study of a chemolithoautotrophic campylobacterium, using Sulfurimonas 26 denitrificans as a model organism. To date, Sulfurimonas denitrificans is the only neutrophilic 27 microorganism with the demonstrated capability of cylcooctasulfur (S8) oxidation. However, the 28 mechanism by which this is accomplished is not known. Our results provide insight into the 29 active metabolism of S. denitrificans, revealing for the first time a differential regulation of the 30 sulfur-oxidation multienzyme complex (SOX) in response to the provided sulfur substrates.