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Considerations on a Laser-Scanning-Microscope With ^ E21954F MICROSCOPICA ACTA JOURNAL OF MICROSCOPIC EQUIPMENT, METHODS AND APPLICATIONS EDITORS R. D. Allen, Hanover/USA-W. Boguth, Basel-L. Jenny, Basel-O. Johari, Chicago E. Kellenberger, Basel • H. Rohr, Basel • W. Stockem, Bonn NUMBER 1 . VOLUME 81 • SEPTEMBER 1978 S. HIRZEL VERLAG STUTTGART Microsc Acta ISSN 0044-376 X MICROSCOPICA ACTA Journal of microscopic equipment, methods and applications formerly Zeitschrift für wissenschaftliche Mikroskopie und mikroskopische Technik Editors: Prof. Dr. R. D. Allen, Prof. Dr. W. Boguth, Prof. Dr. L. Jenny, Dr. O. Johari, Prof. Dr. E. Kellenberger, Prof. Dr. H. Rohr, Prof. Dr. W. Stockem Co-Editiorial Board L. Albert, Karlsruhe • O. von Deimling, Freiburg i. Br. • D. Gerlach, Erlangen • H. Hasel• mann, Tübingen • H. Haug, Lübeck • L. Hottinger, Basel • G. Kiefer, Freiburg i. Br. • A. Läuchli, Hannover • K. Lickfeld, Essen • M.-Th. Mackowsky, Essen • K. Michel, Aalen • H. Mörtel, Erlangen • J. S. Ploem, Leiden • M. Pluta, Warszawa • H. Raith, Dortmund • A. Schaefer, Heerbrugg • Kl. P. SchindT, Wien • F. Thon, Berlin • E. Treiber, Stockholm MICROSCOPIA ACTA publishes reviews, original papers, reports and references to literature covering the whole field of light and electron microscopy with regard to the aspect of micro- scopy technique. MICSROSCOPIA ACTA veröffentlichen Übersichtsbeiträge, Originalarbeiten, Berichte und Literaturhinweise, die das Gebiet der Lichtmikroskopie und der Elektronenmikroskopie unter dem Gesichtspunkt der mikroskopischen Technik behandeln. Contributions will be accepted in English, Ger• MICROSCOPIA ACTA man or French and should be submitted to the Prof. Dr. Louis Jenny, Bäumlihofstr. 375 Acting Etidor: CH-4125 Riehen/Switzerland Manuskripte können in deutscher, englischer oder französicher Sprache abgefaßt sein. Sie Tel. Private: (0 61) 49 59 69 sind bei der Redaktion einzureichen: Institute: (0 61) 43 44 15 Terms of delivery The Journal is published in issues combined to a volume of approximately 450 pages. Sub- scription rate DM 145,— per volume. Orders can be placed with any bookseller or the pub- lisher. Bezugsbedingungen Die Zeitschrift erscheint in einzelnen Heften, die zu Bänden mit etwa 450 Seiten zusammen• gestellt werden. Preis im Abonnement je Band DM 145,—. Bestellungen nimmt jede Buchhandlung im In- und Ausland und der Verlag entgegen. MICROSCOPICA ACTA JOURNAL OF MICROSCOPIC EQUIPMENT, METHODS AND APPLICATIONS 6- EDITORS R. D. Allen, Hanover/USA-W. Boguth, Basel-L. Jenny, Basel-O. Johari, Chicago E. Kellenberger, Basel • H. Rohr, Basel • W. Stockem, Bonn Volume 81 — 1979 — Band 81 S. HIRZEL VERLAG STUTTGART Microsc. Acta ISSN 0044-376 X MICROSCOPICA ACTA Journal of microscopic equipment, methods and applications formerly Zeitschrift für wissenschaftliche Mikroskopie und mikroskopische Technik Editors: Prof. Dr. R. D. Allen, Prof. Dr. W. Boguth, Prof. Dr. L. Jenny, Dr. O. Johari, Prof. Dr. E. Kellenberger, Prof. Dr. H. Rohr, Prof. Dr. W. Stockem Co-Editiorial Board L. Albert, Karlsruhe • O. von Deimling, Freiburg i. Br. • D. Gerlach, Erlangen • H. Hasel• mann, Tübingen • H. Haug, Lübeck • L. Hottinger, Basel • G. Kiefer, Freiburg i. Br. • A. Läuchli, Hannover • K. Lickfeld, Essen • M.-Th. Mackowsky, Essen • K. Michel, Aalen • H. Mörtel, Erlangen • J. S. Ploem, Leiden • M. Pluta, Warszawa • H. Raith, Dortmund • A. Schaefer, Heerbrugg • Kl. P. Schindl, Wien • F. Thon, Berlin • E. Treiber, Stockholm • D. Wittekind, Freiburg i. Br. MICROSCOPICA ACTA publishes reviews, original papers, reports and references to literature covering the whole fleld of light and electron microscopy with regard to the aspect of micro- scopical technique. MICROSCOPICA ACTA veröffentlichen Übersichtsbeiträge, Originalarbeiten, Berichte und Literaturhinweise, die das Gebiet der Lichtmikroskopie und der Elektronenmikroskopie unter dem Gesichtspunkt der mikroskopischen Technik behandeln. Contributions will be aeeepted in English, Ger• MICROSCOPICA ACTA man or French and should be submitted to the Prof. Dr. Louis Jenny, Bäumlihofstr. 375 Acting Editor: CH-4125 Riehen/Switzerland Manuskripte können in deutscher, englischer oder französischer Sprache abgefaßt sein. Sie Tel. Private: (0 61) 49 59 69 sind bei der Redaktion einzureichen: Institute: (0 61) 43 44 15 The issues of Volume 80 appeared: Number 1 September 1978 Number 2 November 1978 Number 3 January 1979 Number 4 March 1979 Number 5 May 1979 © S. Hirzel Verlag Stuttgart 1979 A. Authors of papers Adams, Derek, see Williams, David Agroskin, L. S., see Pevzner, L. Z. Äkerman, Karl, E. 0.: Measurements of membrane potentials using the dye safranine . 147 Bestimmungen von Membranpotentialen mit Hilfe von Safranin Azzam, R. M. A.: Optical detection of cell-surface changes associated with malignant transformations in vitro 313 Optische m-v/'/ro-Feststellung von Zelloberflächenänderungen in Verbindung mit bös• artigen Transformationen Bezler, Hans f, see Leder, Ortwin Bhattacharyya, Tapan Kumar: A dißerential staining technique for vertebrate histology (Short communication) 299 Une methode de coloration differentielle pour l'histologie des Vertebres Boer, Hein, and Woulter H. van Eek: The penetration of the embedding medium methyl methacrylate in undecalcified bone 181 Das Eindringen von Methylmethacrylat als Einbettungsmittel für unentkalkte Kno• chen Borovikov, Yurii S., and Natalia A. Chernogriadskaia: Studies on conformational changes in F-actin of glycerinated muscle fibers during relaxation by means of polarized ultra violet fluorescence microscopy 383 Konformationsänderungen im F-Aktin im Verlauf des Erschlaffens glyzerinisierter Muskelfasern. Untersuchungen mit Hilfe von Fluoreszenzmikroskopie im polarisier• ten UV Chernogriadskaia, Natalia A., see Borovikov, Yurii S. Chun, Moonjin, see Sernetz, Manfred Collins, Barbara Ann, and Edward Ford MacNichol, jr.: Long term fixation for histolog- ical studies (Short communication) 155 Langzeitfixation für histologische Untersuchungen IV Authors of papers Collins, Barbara Ann, and Edward Ford MacNichol, jr.: Sectioning of Epon blocks in the 20 am to 60 um ränge for histological studies (Short communication) .... 227 20—60 um dicke Schnitte von Epon-Blöcken für histologische Untersuchungen Colman, Ondina D., and Juan C. Stockert: Observations on the nucleolar staining by ethanolic phosphotungstic acid (Short communication) 27 Beobachtung über die Nukleolarfärbung durch äthylalkoholische Phosphorwolfram• säure Cremer, Christoph, and Thomas Cremer: Considerations on a laser-scanning-microscope with high resolution and depth of field 31 Überlegungen zu einem Laser-Scanning-Mikroskop mit hoher Auflösung und Schär• fentiefe Cremer, Thomas, see Cremer, Christoph Dewar, Catherine L., and Michael W. Wolowyk: Scanning electron microscopy of blood cells 209 L'examen de leukocytes et d'erythrocytes au microscope ä balayage Dimock, Ronald V., jr., see Hazen, Terry Dutt, Mihir K.: Cytochemical specificity of acridine red towards RNA and depolymerised DNA 189 Cytochemische Spezifität von Akridinrot gegenüber RNS und depolymerisierter DNS Dutt, Mihir K.: Modified Feulgen staining of DNA with aqueous Solution of pinacyanol 195 Modifizierte Feulgen-Färbung für DNS mit wässriger Lösung von Pinacyanol Dutt, Mihir K.: Increased shelf-life and Feulgen staining intensity of a modified trichlor- acetic acid-Schiff reagent 275 Verbesserte Haltbarkeit und Feulgen-Färbungsintensität eines modifizierten Trichlor- essigsäure-Schiff-Reagenz Dutt, Mihir K.: Hoffman's violet and Dahlia as specific stains for animal chromosomes 293 HofTmans Violett und Dahlia als spezifische Farbstoffe für tierische Chromosomen Dutt, Mihir K.: Shelf-life of citric acid-toluidine blue O-SO* and its influence on Feulgen staining 367 Zitronensäure-Toluidinblau 0-SOa: Lagerfähigkeit und Einfluß auf die Feulgen-Fär• bung Dutt, Mihir K.: Detection of DNA in mammalian tissues following removal of RNA with cold phosphoric acid 373 Nachweis von DNS im Gewebe von Säugern nach Entfernung der RNS mit kalter Phosphorsäure Authors of papers V Dutt, Mihir K.: Fixation of mammalian tissues in different fixatives and its inftuence on the staining with methyl green-pyronin 379 Das Fixieren von Säugergewebe in verschiedenen Fixativen und deren Einfluß auf die Färbung mit Methylgrün-Pyronin Eek, Woulter H van, see Boer, Hein Friebe, Bernd: Untersuchungen zum Schwesterchromatidenaustausch bei Seeale cereale 159 Analysis of sister chromatid exchange (SCE) in Seeale cereale Gebhart, Josef, see Roth, Christa Glahn-Luft, Birgit, Peter Schley und Rudolf Wassmuth: Geschlechtsdifferenzierung von neugeborenen Kaninchen mit Hilfe der Karyotypanalyse zu Versuchszwecken (Kurz• mitteilung) 289 Sexual differentiation of newborn rabbits with karyotype analysis for experimental use Gundersen, Hans Jorgen G.: Estimators of the number of objects per area unbiased by edge effects 107 Abschätzungsmethoden für die Objektflächendichte, frei von systematischen Fehlern durch Randeffekte Gupta, A. K., and K. Singh: Influences of primary coma on partially coherent Images: Truncated bar targets 265 Der Einfluß primärer Koma auf die partiell kohärente Abbildung: Abgestufte periodi• sche Strichraster Habermalz, Fritz: Farbmetrische Untersuchungen über die mit Umkehrfarbfilmen dar• stellbaren Farben 137 Colorimetrical investigations of colors produced by color reversal films applied for photomicrography Hannibal-Friedrich, Otto, see Sernetz, Manfred Hartmann, Horst, und Klaus Paradies: Ein Präparationsverfahren für die Auf licht fluo- reszenz-Mikroskopie (Kurzmitteilung)
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