LGMN Antibody Cat. No.: 13-728

LGMN Antibody

Immunofluorescence analysis of C6 cells using LGMN Polyclonal Immunofluorescence analysis of HeLa cells using LGMN Polyclonal Antibody Antibody (13-728) at dilution of (13-728) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of L929 cells using LGMN Polyclonal Antibody (13-728) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Specifications

September 29, 2021 1 https://www.prosci-inc.com/lgmn-antibody-13-728.html HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human, Mouse, Rat

Recombinant fusion containing a sequence corresponding to amino acids 18-323 IMMUNOGEN: of human LGMN (NP_001008530.1).

TESTED APPLICATIONS: IF, WB

WB: ,1:1000 - 1:2000 APPLICATIONS: IF: ,1:50 - 1:200

POSITIVE CONTROL: 1) Mouse lung

2) Mouse liver

PREDICTED MOLECULAR Observed: 54kDa WEIGHT:

Properties

PURIFICATION: Affinity purification

CLONALITY: Polyclonal

ISOTYPE: IgG

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

STORAGE CONDITIONS: Store at -20˚C. Avoid freeze / thaw cycles.

Additional Info

OFFICIAL SYMBOL: LGMN

ALTERNATE NAMES: Legumain, Asparaginyl endopeptidase, Protease, cysteine 1, LGMN, PRSC1

GENE ID: 5641

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

This encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is BACKGROUND: produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a on 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform.

September 29, 2021 2 https://www.prosci-inc.com/lgmn-antibody-13-728.html ANTIBODIES FOR RESEARCH USE ONLY.

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