Table S2: Summary of metabolite ions that showed significant changes in sera of

Lateolabrax japonicus. Ion rank from a loading S-plot of OPLS-DA analysis showed the rank of ions with the highest confidence and greatest contribution to separation between the first and the last group of Lateolabrax japonicus samples collected on October 9 and October 22,

2007, respectively. Ion rank is the same as labeling of ions with relevant m/z in Figure 5b. Fold change equals the fold increase in the level observed in the last group compared with the first group ( Cov. and Cor. >0) or the fold decrease in the level observed in the last group compared with the first group ( Cov. and Cor. <0). VIP (Variable Importance in the Projection), p value

(P value of Independent T Test ), Cor.(correlation), Cov.(covariance) and factor of change of a metabolite were obtained from the OPLS-DA analysis by use of the data sets in positive ion scan mode or negative ion scan mode corresponding to positive ion or negative ion of relevant adduct in the third column respectively.

RT (min)_m/z Identity Adduct * Empirical Mass VIP p value Cor. Cov. Factor of element error Change composition (ppm) Phosphatidylcholine PC with HUFA at the sn-1 position 11.58_852.5534 20:5/22:6 [M+H]+ C50H79NO8P 1.1 9.017 1.49×10-10 0.784 0.261 1.98

11.58_874.5344 [M+Na]+ C50H78NO8PNa 2.2 7.789 6.64×10-8 0.709 0.225 1.59

11.46_896.5462 [M+HCOOH-H]- C51H79NO10P 2.2 6.392 4.74×10-6 0.824 0.165 1.34

11.47_836.5262 [M-CH3]- C49H75NO8P 3.8 4.949 1.42×10-6 0.838 0.128 1.36

11.84_878.5715 22:6/C22:6 [M+H]+ C52H81NO8P 1.7 7.209 9.62×10-12 0.855 0.209 2.01

11.86_900.5538 [M+Na]+ C52H80NO8PNa 2.1 7.278 2.93×10-10 0.802 0.211 1.69

11.70_922.5620 [M+HCOOH-H]- C53H81NO10P 2.4 5.842 2.58×10-7 0.801 0.151 1.37

11.70_862.5407 [M-CH3]- C51H77NO8P 2.3 3.896 1.36×10-6 0.789 0.101 1.34

12.10_854.5734 20:4/22:6 [M+H]+ C50H81NO8P 4 4.466 6.50×10-11 0.806 0.129 1.82

12.10_876.5531 [M+Na]+ C50H80NO8PNa 1.4 3.729 2.51×10-6 0.655 0.108 1.41

11.98_898.5619 [M+HCOOH-H]- C51H81NO10P 1.2 3.637 1.01×10-5 0.751 0.094 1.28

11.82_828.5583 20:4/20:5 [M+H]+ C48H79NO8P 4.8 2.853 1.19×10-3 0.576 0.081 1.42 PC with SFA or MUFA at the sn-1 position 13.06_780.5544 16:0/20:5 [M+H]+ C44H79NO8P 0.1 6.218 1.35×10-3 0.455 0.18 1.26

13.58_854.5660 18:1/22:6 [M+Na]+ C48H82NO8PNa 1.9 4.737 1.08×10-3 -0.487 -0.137 1.25

13.48_806.5724 16:0/22:6 [M+H]+ C46H81NO8P 3 4.356 3.03×10-2 0.369 0.126 1.07

13.12_850.5629 [M+HCOOH-H]- C47H81NO10P 3.6 5.885 2.51×10-2 -0.317 -0.152 1.24

13.14_790.5414 [M-CH3]- C45H77NO8P 3.4 4.989 1.46×10-2 -0.334 -0.129 1.27 12.26_778.5386 16:1/20:5 [M+H]+ C44H77NO8P 0.1 4.114 8.09×10-4 0.495 0.119 1.27

14.20_804.5517 16:1/22:6 [M+H]+ C46H79NO8P 3.2 3.233 4.39×10-2 -0.286 -0.094 1.26

15.98_804.5770 16:0/18:1 [M+HCOOH-H]- C43H83NO10P 2 3.176 1.11×10-3 -0.623 -0.082 2.77 PC with plasmalogen at the sn-1 position 14.52_814.5707 p-16:0/22:5 [M+Na]+ C46H82NO7PNa 2.3 7.24 1.50×10-4 -0.583 -0.21 1.50

14.54_792.5902 [M+H]+ C46H83NO7P 0.6 4.501 8.82×10-4 -0.493 -0.13 1.38

14.36_836.5828 [M+HCOOH-H]- C47H83NO9P 2.6 5.891 1.06×10-4 -0.676 -0.152 1.30

14.36_776.5603 [M-CH3]- C45H79NO7P 1.2 4.904 2.12×10-5 -0.73 -0.127 1.32

14.51_840.5905 p-18:0/22:6 [M+Na]+ C48H84NO7PNa 2.6 2.833 4.24×10-4 -0.574 -0.08 1.26

Lysophosphatidyl choline

6.69_568.3403 22:6 [M+H]+ C30H51NO7P 0.0 3.526 3.7×10-10 0.783 0.102 1.71

6.59_552.3082 [M-CH3]- C29H47NO7P 1.4 8.938 4.18×10-13 0.865 0.231 2.00

6.59_612.3306 [M+HCOOH-H]- C31H51NO9P 0.8 3.239 1.96×10-10 0.820 0.084 1.95

5.97_526.2932 20:5 [M-CH3]- C27H45NO7P 0.4 4.474 1.41×10-12 0.882 0.116 2.29

6.71_528.3084 20:4 [M-CH3]- C27H47NO7P 1.1 3.296 1.43×10-12 0.841 0.085 2.13 Cholic acid metabolite 2.69_554.2540 taurocholic acid [M+K]+ C26H45NO7SK 2.5 3.865 6.08×10-2 -0.455 -0.112 1.97

2.70_514.2826 [M -H]- C26H44NO7S 2.5 3.467 2.41×10-2 -0.32 -0.09 1.81