Supplementary Table 1 Forward and Reverse SRAP Primers Used in the Present Study

Supplementary Table 1Forward and reverse SRAP primers used in the present study

Primer / Forward Sequence / Primer / Reverse Sequence
Me1: / TGAGTCCAAACCGGATA / Em1: / GACTGCGTACGAATTAAT
Me2: / TGAGTCCAAACCGGAGC / Em2: / GACTGCGTACGAATTTGC
Me3: / TGAGTCCAAACCGGAAT / Em3: / GACTGCGTACGAATTGAC
Me4: / TGAGTCCAAACCGGACC / Em4: / GACTGCGTACGAATTTGA
Me5: / TGAGTCCAAACCGGAAG / Em5: / GACTGCGTACGAATTAAC
Me6: / TGAGTCCAAACCGGTAA / Em6: / GACTGCGTACGAATTGCA
Me7: / TGAGTCCAAACCGGTCC / Em7: / GACTGCGTACGAATTGAG
Me8: / TGAGTCCAAACCGGTGC / Em8: / GACTGCGTACGAATTGCC
Em9 / GACTGCGTACGAATTTCA

Supplementary Table 2 Polymorphism revealed by 55 SRAP primer combinations

Primer combination / Total bands / Polymorphic bands / Percentage of polymorphic bands
Me1/Em1 / 5 / 5 / 100
Me1/Em2 / 7 / 7 / 100
Me1/Em3 / 4 / 3 / 75
Me1/Em4 / 8 / 8 / 100
Me1/Em5 / 6 / 6 / 100
Me1/Em6 / 5 / 3 / 60
Me1/Em7 / 4 / 4 / 100
Me1/Em8 / 4 / 3 / 75
Me1/Em9 / 6 / 6 / 100
Me2/Em1 / 7 / 7 / 100
Me2/Em2 / 10 / 10 / 100
Me2/Em3 / 9 / 8 / 88.89
Me2/Em4 / 7 / 7 / 100
Me2/Em6 / 9 / 8 / 88.89
Me2/Em7 / 7 / 6 / 85.71
Me2/Em9 / 10 / 9 / 90
Me3/Em1 / 8 / 8 / 100
Me3/Em2 / 4 / 4 / 100
Me3/Em3 / 6 / 6 / 100
Me3/Em4 / 10 / 8 / 80
Me3/Em5 / 7 / 7 / 100
Me3/Em6 / 8 / 7 / 87.50
Me3/Em7 / 7 / 7 / 100
Me3/Em8 / 5 / 5 / 100
Me3/Em9 / 4 / 3 / 75
Me4/Em1 / 5 / 5 / 100
Me4/Em2 / 4 / 3 / 75
Me4/Em3 / 3 / 3 / 100
Me4/Em4 / 4 / 4 / 100
Me4/Em5 / 4 / 4 / 100
Me4/Em6 / 5 / 5 / 100
Me4/Em7 / 7 / 7 / 100
Me4/Em8 / 5 / 5 / 100
Me4/Em9 / 6 / 6 / 100
Me5/Em1 / 9 / 9 / 100
Me5/Em2 / 7 / 7 / 100
Me5/Em3 / 7 / 7 / 100
Me5/Em4 / 6 / 5 / 83.33
Me5/Em5 / 9 / 7 / 77.78
Me5/Em6 / 5 / 5 / 100
Me5/Em8 / 2 / 2 / 100
Me5/Em9 / 4 / 4 / 100
Me6/Em1 / 6 / 6 / 100
Me6/Em2 / 4 / 3 / 75
Me6/Em3 / 4 / 4 / 100
Me6/Em5 / 9 / 9 / 100
Me6/Em6 / 8 / 7 / 87.50
Me6/Em7 / 7 / 7 / 100
Me8/Em3 / 6 / 6 / 100
Me8/Em4 / 7 / 7 / 100
Me8/Em5 / 7 / 7 / 100
Me8/Em6 / 8 / 8 / 100
Me8/Em7 / 6 / 5 / 83.33
Me8/Em8 / 9 / 9 / 100
Me8/Em9 / 8 / 8 / 100
Total / 348 / 329 / 94.326
Average / 6.33 / 5.98 / 94.326

Supplementary Table 3 Intrapopulation gene diversity of 14M. alba subpopulations belonging to three populations from the Ladakh region

Population/Subpopulation / Sample size / H
(mean±SD) / I
(mean±SD) / NPL / PPL
Indus
SKB / 4 / 0.1555±0.2112 / 0.2254±0.2991 / 132 / 37.93
DM / 4 / 0.1460±0.2032 / 0.2131±0.2906 / 127 / 36.49
ACH / 4 / 0.1447±0.1967 / 0.2142±0.2829 / 134 / 38.51
BM / 4 / 0.1406±0.2069 / 0.2025±0.2943 / 115 / 33.05
SJ / 4 / 0.1114±0.1880 / 0.1625±0.2699 / 96 / 27.59
Suru
BT / 4 / 0.1729±0.2176 / 0.2495±0.3075 / 144 / 41.38
AKM / 4 / 0.1855±0.2032 / 0.2753±0.2915 / 173 / 49.71
PS / 4 / 0.2027±0.2155 / 0.2961±0.3047 / 178 / 51.15
LO / 4 / 0.1724±0.2068 / 0.2535±0.2956 / 155 / 44.54
Nubra
CH / 4 / 0.0828±0.1687 / 0.1215±0.2419 / 74 / 21.26
BK / 4 / 0.1182±0.1947 / 0.1717±0.2771 / 101 / 29.02
TU / 4 / 0.1534±0.2090 / 0.2226±0.2973 / 130 / 37.36
TY / 4 / 0.1309±0.1975 / 0.1914±0.2823 / 115 / 33.05
HU / 4 / 0.1316±0.1913 / 0.1951±0.2762 / 122 / 35.06

Nei’s gene diversity(H), Shannon’s information index (I), number of polymorphic loci (NPL), percentage of polymorphic loci (PPL), collection site codes as in Table 1