Protocol Type
- Contacts
Primary Investigator / Alternate contact
Name / Click here to enter text. / Name / Click here to enter text. /
Email / Click here to enter text. / Email / Click here to enter text. /
Dept / Click here to enter text. / Dept / Click here to enter text. /
Telephone / Click here to enter text. / Telephone / Click here to enter text. /
After hours / Click here to enter text. / After hours / Click here to enter text. /
PROTOCOL FOR ANIMAL USE AND CARE / Score 1:
Score 2:
Total:
- Title:
- Protocol Type:
- Species
Common Names / Total number for study / Source of animals
Common means common—rat, mouse, dog, cat, cow, etc. / Make sure all numbers match with 13 e and f / “Approved vendor” or “<species> colony” /
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5. Procedures
This is for the lay person. Include why, and all procedures, especially anything that could change the appearance or behavior of the animal. /
6. Animal Location(s)
Overnight Housing (Vivarium) / Study Area/Laboratory / Animals Maintained by
<species> colony / N/A unless you state you are moving them outside the facility / Vivarium /
7. Special Husbandry Requirements
Only things that differ from normal procedures /
8. Will hazardous materials be used in this study? No
If yes, of what nature? Choose an item.
9. Special Procedures and/or Activities (check all that apply) None ☐
☐Anesthetics or Tranquilizers
☐Survival Surgical Procedures
☐Terminal Surgical Procedures
☐Neuromuscular Blocking Agents / ☐Prolonged Restraint
☐Food/Water Regulation
☐Death as an Endpoint
☐Single housing of social species
10. Funding and Funding Sources
Animal Science Teaching
11. What veterinarian or veterinary service will provide care for your animals?
Choose an item. /
12. Objectives and Significance
Objectives
Convince me! Make sure you include enough background information that I can understand why you are doing this. Then clearly define what you are doing, what you expect, and what it means. Define acronyms and keep it at a level a scientist in any field would understand. /
Significance
Significance can be explained as beneficial to humans, animals, benefits society, or advances our knowledge and understanding. /
13. Literature search for alternatives and unnecessary duplication:
a) Databases (minimum 2 databases):
Database name / Years covered / Keywords/Search Strategy / Date(s)
Include at least 2 scientific databases / Click here to enter text. / Click here to enter text. / Click here to enter text. /
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13 b. Results of the Literature Search:
Explain any alternatives and why you chose your methods. /
13 c. Has this study been previously conducted?
Answer should be no. /
13 d. Species Rationale:
If you are not working with the animal model that will benefit from the research, then you need to justify that the species is appropriate. You can do this through a combination of physiological and/or behavioral comparisons and historical data. /
13 e. Animal Numbers Justification
Animal number justification is a 2-fold process.
You must justify the number of groups needed.
You must justify the number of animals per group—this will usually be based on past research since I am not asking you to do a statistical analysis, but you numbers should be statistically significant.
N = n (the number per group) x the number of total groups /
13 f. Study Groups and Numbers Table: (if you need additional rows, let me know)
Group / Species / Number of Animals / Procedures/Treatments
1, 2, 3
A, B, C
Or a combination / Click here / Must match numbers justification / Each group must be unique and all experimental procedures should be listed. /
Click here / Click here / Click here / If breeding animals, you will need separate groups for females, males, and offspring. Estimate offspring to be born and include all offspring, not just those needed for the experiment. Offspring not used can be in their own group separate from any used in the experiment. /
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14 a. Summary of Procedure
Describe the use of animals in your project from receipt of animals to final desposition.
When filling out your summary of procedures, think about the following:
Include all procedures from start (acquisition) to finish (return of the animals to the colony or euthanasia).
Include a timeframe and endpoint.
Injections require dose, route, and frequency. Dose can be a range from the formulary.
Blood collections require volume, route, and frequency.
You do not need a detailed description of the analyses done on blood or tissue, but you do need to describe the collection of the blood and tissue and the purpose for the collection.
Describe procedures used in surgery. I don’t need step-by-step instructions, but enough detail to know that you are using aseptic procedures, you know what you are doing, and what you are doing is appropriate.
Describe breeding procedures you are using if producing offspring. All young produced as part of the experiment must be included in 13f and the total animal count.
Describe in detail any unique apparatus (mazes, arenas, special caging, and restraint devices).
Behavior tests should have a clear description of how long you are observing each animal and what behaviors you are looking for (ethogram). Keep in mind animals may not exhibit the desired behavior.
Remember to include control groups; utilize sham procedures and administration of vehicles.
Make it logical. Avoid too much redundancy. Use paragraphs, bullet points, or tables as appropriate.
Keep it simple—one or two variables. /
14 b. Drugs to be used (except for euthanasia). Include anesthetics, analgesics, neuromuscular blocking agents, antibiotics, and/or experimental compounds. (If you need additional rows, let me know)
Species / Drug / Dose / Route / When and how often will it be given?
Click here. / (Injectable drugs) / Mg/kg
From formulary; you can use a range. / IP, SQ, ID, etc. / “Once, to effect” is common for injectable anesthetics. Otherwise, you may use One procedure, once a day, twice a day, etc. /
Click here. / (Inhalant drugs) / To effect / Inhalation / Click here to enter text. /
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14 c. Anesthesia monitoring
Rodents are discussed in class. Larger animals will need monitor of blood pressure, heart rate, etc. /
14 d. Post-Anesthesia Monitoring
This is monitoring while recovering from anesthesia. Should be a minimum of every 15 minutes. Explain what physiological parameters you are monitoring. /
14 e. Surgery
i) Location
Building / Room / Surgeon (s)
Rodents can be Cole B surgical room
Larger animals can be TRACS surgical suite. / Click here to enter text. / Click here to enter text. /
ii) Post-Surgical Monitoring
a)Please identify the parameters monitored, and the interval(s) and for what duration of monitoring
This refers to the period between recovery from anesthetics and healing of the surgical site, usually the removal of wound clips or sutures (7 – 10 days). This should be a minimum of once per day. Describe what physiological parameters you are monitoring. It should include observations of both the surgical site and the animal as a whole. /
b)When will analgesics be administered and at what interval(s)?
Analgesics are required unless withholding them is justified. Intervals should be appropriate for the drug used. /
c)If post-operative analgesics cannot be given, please provide scientific justification.
Click here to enter text. /
15. Adverse Effects
a)Describe all significant adverse effects that may be encountered during the study.
This should describe the adverse effects. Be thorough. Very few studies can say none are expected. /
b)Describe frequency for monitoring the well-being of animals on the study and criteria for terminating/modifying the procedure(s) if adverse effects are observed.
This section asks 2 questions.
1)How often are the animals monitored and what you are monitoring for?
2)When would you change the experimental design due to unexpected adverse effects? /
c)How will the signs listed above be ameliorated or alleviated?
Going back to section a, address each expected adverse effect and explain anything you are doing to lessen the pain and distress. /
d)List the criteria to be used to determine when euthanasia is to be performed or the animal will be removed from the study.
Enter the physiological criteria for euthanasia. Use such signs as weight loss and body condition if appropriate. Euthanasia here and in 16 must always be an option. We must prove we know what to do if animals are injured or become ill, even if not a part of the experiment. /
16. Methods of Euthanasia (If you need additional rows, let me know)
Species / Method / Drug / Dose / Route / Justification if physical method is being used.
Required—even if the animals are not expected to be euthanized. / Overdose / “injectable drugs” / Mg/kg / Usually IV or IC / Only required for physical methods used as primary euthanasia; however, you may use this box to explain other things like anesthesia prior to IC injection. /
Click here. / Overdose / “inhalant drugs” / To effect / Inhalation / Click here to enter text. /
Click here. / “Physical methods” / N/A / N/A / N/A / Justify /
Click here. / Click here. / Click here. / Click here. / Click here. / Click here to enter text. /
17. Disposition of Animals
What happens to animals after the experiment? Generally 2 options.
All animals will be euthanized.
Animals will be transferred to another approved protocol. /