JOHNS HOPKINS UNIVERSITY Department of Biology

Instructor Name:
Robert D. Horner, Ph.D. / Course Title: Biochemistry Laboratory
Instructor Contact Information
179 UTL, Department of Biology
410-516-8067 or -7281 / Course Number: 020.315.21
Distribution: N
Instructor Office Hours/Location: Tues. & Thurs. 1-2 p.m., 179 UTL . / Credits: 2
Lab Lecture: M, W, F noon -1:00 p.m. in Mudd 100
Lab Section: M, W, F 1:30 -4:30 p.m. in 174 UTL . / Dates: June 27 – July 29, 2015
COURSE DESCRIPTION
This course will reinforce the topics presented in Biochemistry 020.305 through laboratory exercises which use quantitative measurement to study cellular components. Topics include pH, proteins, carbohydrates, lipids, nucleic acids, and enzymes.
COURSE LEARNING OBJECTIVES
Students should obtain a good working knowledge and basic understanding of the following areas:
1 / Buffers, pH and weak acids in proteins and nucleic acids
2 / Use of spectrophotometry to measure biomolecules and biochemical processes
2 / Enzyme catalysis, purification, quantitation and electrophoretic analysis
3 / Measurement of binding constants and free energy of protein unfolding
4 / Chemical characterization of carbohydrates and lipids
5 / DNA isolation and restriction enzyme analysis
6 / Polymerase chain reaction
REQUIRED MATERIALS
TEXTBOOKS: 1. Manual online at course website 2. Berg, J.M., Tymoczko, J.L., & Stryer, L., Biochemistry, 7th Ed.
SUPPLIES: Experimental Research Notebook with carbonless duplicating pages.
COURSE WEBSITE: on Blackboard / https://blackboard.jhu.edu/webapps/portal/frameset.jsp
EVALUATION AND GRADING
Grades will be based on the following assessments:
ASSESSMENT TYPE / PERCENT OF FINAL GRADE
Several class assessments during lecture / 2%
Twelve lab reports / 11%
Two midterm exams / 37%
Twelve quizzes, one at the beginning of each lab / 22%
Lab practical examination / 28%
ATTENDANCE POLICY
Students are required to attend all lab and lecture sessions.

JOHNS HOPKINS POLICIES AND SUPPORT SERVICES

This course is governed by the policies set forth in The Johns Hopkins University Undergraduate Student Handbook, which contains information on a wide variety of topics, such as support services, and policies relating to student rights and responsibilities. This course is governed by the policies set forth in this document.

Some JHU student support services you may find useful include:

SUPPORT SERVICE / LOCATION / PHONE NUMBER / WEBSITE
Library E-Reserves / http://www.library.jhu.edu/services/forms/reserves.html /
The Learning Den
provides tutoring for all registered students in Arts and Sciences and Engineering. No fee and no limit to the number of sessions a student may attend. / Homewood Campus in Gilman Hall and nearby buildings /
Ms. Anne Benner
Garland Hall, Suite 300
410-516-8216
Summer & Intersession Programs / 3505 N. Charles Street, Suite 101 / 410-516-4548
http://www.jhu.edu/intersession

CLASSROOM ACCOMODATIONS FOR STUDENTS WITH DISABILITIES

If you are a student with a documented disability who requires an academic adjustment, auxiliary aid or other similar accommodations, please contact The Office of Student Disability Services at ,

call 410-516-4720 or visit 385 Garland Hall.

STATEMENT OF DIVERSITY AND INCLUSION

Johns Hopkins University is a community committed to sharing values of diversity and inclusion in order to achieve and sustain excellence. We believe excellence is best promoted by being a diverse group of students, faculty and staff who are committed to creating a climate of mutual respect that is supportive of one another’s success. Through its curricula and clinical experiences, we purposefully support the University’s goal of diversity, and in particular, work toward an ultimate outcome of best serving the needs of students. Faculty and candidates are expected to demonstrate an understanding of diversity as it relates to planning, instruction, management, and assessment.

A WORD ON ETHICS

Ethics: Because the strength of the university depends on academic and personal integrity, the Course Instructor of this course expects students to be follow the code of ethics established by the undergraduate students of the Johns Hopkins University in 1975. In this course, you must be honest and truthful. Ethical violations include cheating on exams, plagiarism, reuse of assignments, improper use of the Internet and electronic devices, unauthorized collaboration, alteration of graded assignments, forgery and falsification, lying, facilitating academic dishonesty, and unfair competition. Ignorance of these rules is not an excuse. The university will punish cheaters with failure on an assignment, failure in a course, permanent transcript notation, suspension, and/or expulsion. Offenses may be reported to medical, law or other professional or graduate schools when a cheater applies.

In addition, the specific ethics guidelines for this course are:

Quizzes and the final practical exam are closed book.

The only calculators allowed during test periods are TI-30 series calculators. Calculators may not be shared between students during quizzes or the practical exam.

Lab reports must be the product of a student=s own effort.

Report any violations you witness to the instructor. You may consult the associate dean of students and/or the chairman of the Ethics Board beforehand. See the guide on AAcademic Ethics for Undergraduates@ and the Ethics Board web site (http://ethics.jhu.edu) for more information.

HELPFUL ADVICE

For suggestions on lecture note-taking, please Google “Cornell note-taking”.

For a productive text reading method, please Google “SQ3R”.

This course covers in four weeks what is normally covered in the 13 weeks of a semester. If you do not have the prerequisites of this course, you will be at risk for failure.

A course website has been constructed on Blackboard. Abbreviated lectures will be posted. If you are registered in the course, your JHED ID and password will give you access. The URL is https://blackboard.jhu.edu/webapps/portal/frameset.jsp

The instructor and teaching assistant will monitor the discussion board, but since the course meets three times a week, questions are best asked in lecture and lab so everyone will hear and benefit.

COURSE SCHEDULE

Day Lab Lecture

6/27 1. pH, Buffers, Amino Acids Intro., pH & Spectrophotometry

6/29 2. Spectrophotometry Protein Folding

7/1 3. Protein Folding Protein-Ligand Binding

7/4 4th of July Holiday, no lab no lecture

7/6 4. Protein-Ligand Binding Enz. Catalysis

7/8 5. Enzyme Catalysis Carbohyd. Chem.

7/11 6. Carbohydrate Chemistry Lipids & Protein Purif.

7/13 7. Lipids Midterm Exam I (labs 1-5)

7/15 8. Protein Purification Lysozyme Enzyme Mech

7/18 9. Lysozyme Enzyme Mechanism Prot. Electrophor.

7/20 10. Protein Electrophoresis DNA Isolation & PCR & Restriction Enz.

7/22 11. DNA Isolation & PCR Midterm exam II (labs 6-9)

7/25 12. Restriction Enzymes Review

7/27 13. Practical Exam no lecture