US010731168B2 (12 ) United States Patent ( 10 ) Patent No.: US 10,731,168 B2 Landowski et al. (45 ) Date of Patent: * Aug. 4 , 2020 ( 54 ) PROTEASE DEFICIENT FILAMENTOUS ( 2013.01) ; CO7K 2317/622 (2013.01 ) ; COOK FUNGAL CELLS AND METHODS OF USE 2317/94 (2013.01 ) ; YO2P 20/52 (2015.11 ) THEREOF (58 ) Field of Classification Search ( 71) Applicant: GLYKOS FINLAND OY , Helsinki None (FI ) See application file for complete search history . ( 72 ) Inventors : Christopher Landowski, Helsinki (FI ) ; Anne Huuskonen , Helsinki (FI ) ; ( 56 ) References Cited Juhani Saarinen , Helsinki (FI ) ; Ann U.S. PATENT DOCUMENTS Westerholm - Parvinen , Kirkkonummi (FI ) ; Anne Kanerva , Helsinki (FI ) ; 4,758,512 A 7/1988 Goldberg et al. 5,674,728 A 10/1997 Buxton et al . Jari Natunen , Vantaa (FI ) ; Anna - Liisa 5,693,520 A 12/1997 Branner et al. Hänninen , Helsinki (FI ) ; Noora 5,756,338 A 5/1998 Buxton et al. 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C12N 15/80 ( 2006.01 ) CO7K 16/28 (2006.01 ) (Continued ) COZK 16/00 ( 2006.01) COZK 14/65 ( 2006.01 ) Primary Examiner Suzanne M Noakes CO7K 14/61 ( 2006.01 ) CO7K 14/56 ( 2006.01 ) Assistant Examiner Jae W Lee C12N 9/58 (2006.01 ) ( 74 ) Attorney , Agent, or Firm — Patrick J. Halloran C12P 21/02 ( 2006.01 ) C12P 21/00 ( 2006.01 ) (52 ) U.S. CI. (57 ) ABSTRACT CPC C12N 15/80 (2013.01 ) ; C07K 14/56 ( 2013.01 ) ; CO7K 14/61 (2013.01 ) ; CO7K 14/65 The present disclosure relates to compositions and methods (2013.01 ) ; COOK 16/00 (2013.01 ) ; CO7K useful for the production of heterologous proteins in fila 16/2887 ( 2013.01 ) ; C12N 9/58 ( 2013.01) ; mentous fungal cells . C12P 21/005 (2013.01 ) ; C12P 21/02 (2013.01 ) ; CO7K 2317/14 ( 2013.01) ; CO7K 2317/24 (2013.01 ) ; CO7K 2317/41 (2013.01 ) ; 16 Claims, 46 Drawing Sheets CO7K 2317/55 (2013.01 ) ; CO7K 2317/569 Specification includes a Sequence Listing . US 10,731,168 B2 Page 2 ( 56 ) References Cited Kubicek , et al . 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