291st OMICS International Conference International Conference on Aquaculture & Fisheries July 20-22, 2015 Brisbane, Australia Posters Aquaculture-2015 Page 95 Shann-Tzong Jiang, J Aquac Res Development 2015, 6:6 http://dx.doi.org/10.4172/2155-9546.S1.003 International Conference on Aquaculture & Fisheries July 20-22, 2015 Brisbane, Australia Production of functional peptides using fish protein hydrolysates with inhibition ability against angiotensin I-converting enzyme using E. coli expression system Shann-Tzong Jiang National Taiwan Ocean University, Taiwan Background: There are various angiotension-I converting enzyme inhibitors (ACEIs) have been investigated for palliating the hypertension. Methods: To produce ACE inhibitor peptides, 15 fragments of DNA sequences encoded well-known ACEI peptides from fish protein hydrolysates, GW, IW, KW, LF, MF, VW, VY, YL, GPL, GPM, IKW, VY, IRPVQ, IWHHT and IYPRY, were designed as a combinative DNA encoded a fusion ACEI polypeptide which could be hydrolyzed into individual ACEI peptides by chymotrypsin. The combinative DNA consisted of 234 nucleotides was cloned into the pET-23a(+) expression vector and then transformed into E. coli BL21(DE3) expression host. Results: After 8 h induction by 0.1 mM isopropyl-β-D-thiogalactopyranoside, high activity of the recombinant fusion ACEI polypeptide was expressed. After sonication to disrupt the cell wall, the recombinant fusion ACEI polypeptide could be purified using Ni Sepharose™ 6 Fast Flow. The IC50 value of recombinant ACEI polypeptide is 11.82 μM. After chymotrypsin digestion, a 74-fold increase of ACEI activity (0.16 μM) was obtained, which was equivalent to 0.022 μM of captopril. Conclusion: The ACEI activity of the chymotrypsin hydrolysate increased about 74-fold activity after hydrolysis. It can be used in the health food for the prevention of high blood pressure, and even the development of drugs for medication in the future. Biography Shann-Tzong Jiang earned his Master Degree from Tokyo University of Fisheries, Japan, and PhD from University of Rhode Island, USA. He is a Fellow of the International Academy of Food Science & Technology, and National Endowed Chair Professor of the Ministry of Education of Taiwan. Currently he is working at the Department of Food Science, National Taiwan Ocean University, and at Providence University as Senior Vice President. He has published more than 170 papers in reputed journals and has been serving as Editorial Board Member of several repute journals. [email protected] [email protected] [email protected] Notes: J Aquac Res Development Volume 6, Issue 6 ISSN: 2155-9546 JARD, an open access journal Aquaculture-2015 July 20-22, 2015 Page 96 Myung-Hwa Jung, J Aquac Res Development 2015, 6:6 http://dx.doi.org/10.4172/2155-9546.S1.003 International Conference on Aquaculture & Fisheries July 20-22, 2015 Brisbane, Australia Effects of protective immunity water temperature on mortality in Rock Bream Iridovirus (RBIV) infected rock bream (Oplegnathus fasciatus) and survivors obtain Myung-Hwa Jung Chonnam National University, Republic of Korea ock Bream Iridovirus (RBIV) causes huge losses especially in rock bream Oplegnathus fasciatus. Rock bream injected with RBIV Rand held at 29, 26, 23 or 20°C had 100% mortality. Conversely, all infected fish held at 17°C survived even after the temperature was progressively increased to 26°C at 100 days post infection (dpi). Rock bream exposed to virus and held for 2, 4 and 7 days at 23/26°C before the temperature was reduced to 17°C had mortality rates of 26.6/73.2%, 66.6/100% and 93.4/100% respectively through 100 dpi. When surviving fish had the water temperature increased from 17 to 26°C at 100 dpi, they did not exhibit signs of disease and had low virus copy numbers (below 103). To investigate the development of a protective immunity, rock bream were infected with RBIV and held at 23°C before shifting the water temperature to 17°C at 4 dpi. All injected fish survived until 120 dpi. While 100% of the previously unexposed fish died, 80.2% of the previously infected fish survived. When the survivors were re- challenged again at 160 dpi, no further mortality occurred. The high survival rate of fish following re-challenge with RBIV indicates that protective immunity was established in the surviving rock bream. Biography Myung-Hwa Jung is currently working in Chonnam National University, South Korea in the Department of Aqualife Medicine. [email protected] Notes: J Aquac Res Development Volume 6, Issue 6 ISSN: 2155-9546 JARD, an open access journal Aquaculture-2015 July 20-22, 2015 Page 97 Aihua Li et al., J Aquac Res Development 2015, 6:6 http://dx.doi.org/10.4172/2155-9546.S1.003 International Conference on Aquaculture & Fisheries July 20-22, 2015 Brisbane, Australia Transcriptomic and proteomic analysis of splenic immune mechanisms of rainbow trout (Oncorhynchus mykiss) infected by Aeromonas salmonicida subsp. salmonicida Aihua Li1, Meng Long1, Juan Zhao2, Tongtong Li, Carolina Tafalla3, Qianqian Zhang1, Xiehao Wang1, Xiaoning Gong1 and Zhixin Shen2 1Chinese Academy of Sciences, China 2Qinghai Provincial Fishery Environmental Monitoring Center, China 3Centro de Investigación en Sanidad Animal (CISAINIA), Spain urunculosis caused by Aeromonas salmonicida subsp. salmonicida is an epidemic disease among salmonids including rainbow Ftrout (Oncorhynchus mykiss). However, the immune mechanisms that are elicited in rainbow trout against the invasion of A. salmonicida are not yet fully understood. In this study, we examined the spleen to investigate the immune response of rainbow trout at 3d post infection by A. salmonicida at the transcriptome and proteome levels by using Illumina-seq and iTRAQ methods, respectively. A total of 1036 genes and 133 proteins were found to undergo differential expression during the immune response of the spleen against A. salmonicida infection. Gene ontology and KEGG analysis were conducted among the differentially expressed genes and proteins revealing that immune system process and response to stimulus were the top two biological processes and immune system, signaling molecules and interaction and immune diseases were the differential pathways activated. Correlation analysis of transcriptomic and proteomic results showed 17 proteins (11 up-regulated and 6 down-regulated) having consistent expression at RNA and protein levels. Moreover, protein–protein interaction analysis showed that diseases, proteasome, aminoacyl- tRNA biosynthesis and nucleotide metabolism were the main interactions among the consistently expressed proteins. Consequently, these up-regulated proteins namely ferritin, CD209, IL13Rα1, VDAC2, GIMAP7, PSMA1 and two ANXA11s could be considered as potential biomarkers for rainbow trout immune responses. Biography Aihua Li is currently a Professor in Institute of Hydrobiology, Chinese Academy of Sciences, China. [email protected] Notes: J Aquac Res Development Volume 6, Issue 6 ISSN: 2155-9546 JARD, an open access journal Aquaculture-2015 July 20-22, 2015 Page 98 An-Chin Lee, J Aquac Res Development 2015, 6:6 http://dx.doi.org/10.4172/2155-9546.S1.003 International Conference on Aquaculture & Fisheries July 20-22, 2015 Brisbane, Australia A study on microalgae filtered and digested by hard clam (Meretrix lusoria) An-Chin Lee National Chia-Yi University, Taiwan icroalgae are widely considered as the major component of the feed fed by hard clam. However, the information Mabout which concentrations of microalgae inducing the pseudo feces production of hard clam and the digestibility of microalgae by hard clam is limited. Two species of microalgae, Tetraselmis chui and Isochrysis galbana are used in this study. Five concentrations of T. chui and ten concentrations of I. galbana were used in the experiment of pseudo feces production of hard clam. The concentrations of T. chui and I. galbana inducing 50% population of hard clam to produce pseudo feces are 9300 cells/ mL and 95000 cells/mL, respectively. Feces collection was carried out in four time periods, 0-3 hour feeding period, 0-4, 4-8 and 8-20 hours period after hard clam transferred to clean sea water. Protein content and carbohydrate content in the feces of hard clam fed with T. chui and I. galbana in 0-4 hour period after hard clam transferred to clean sea water were the maximal. The digestibility of microalgal protein/carbohydrate by hard clam was evaluated by the ratio of the amount of protein/carbohydrate in feces collection divided by that in microalgae fed by hard clam. Protein and carbohydrate digestibility of T. chui by hard clam were 52±2.6% and 70±8%, respectively. Protein and carbohydrate digestibility of I. galbana by hard clam were 81.3±2.3% and 88.1±2.2% respectively. Biography An-Chin Lee earned his Master Degree from National Taiwan Ocean University, Taiwan and PhD from University of Maryland at College Park, USA. Currently, he is working at the department of Aquatic Biosciences, National Chia-Yi University, Taiwan and also at NCYU as Secretary General. He paid his attention to the project of anaerobic metabolism of hard clam (Meretrix lusoria) for many years. He is also interested in looking at filtering behavior of hard clam and will try to prepare supplemental diet of hard clam. [email protected] Notes: J Aquac Res Development Volume 6, Issue 6 ISSN: 2155-9546 JARD, an open access journal Aquaculture-2015 July 20-22, 2015 Page 99 Mong Fong Lee, J Aquac Res Development 2015, 6:6 http://dx.doi.org/10.4172/2155-9546.S1.003 International Conference on Aquaculture & Fisheries July 20-22, 2015 Brisbane, Australia Spawning habitat selection of Pharaoh cuttlefish, Sepia pharaonis (Cephalopoda: Sepiidae) in artificial culture Mong Fong Lee National Penghu University of Science and Technology, Taiwan ephalopods are one of the potential candidates of aquaculture species in the future. Pharaoh cuttlefish, Sepia pharaonis, is Cone of important fishery resources in southeastern Asia. Spawning habitat of aquatic species is crucial for successful seed production.