Mechanisms of Eukaryotic Transcription Session 1 INITIATION COMPLEXES TUESDAY 8/27/2013, 7:30 PM P. Cramer # lname Title Talk Length 1 Nogales Visualizing core promoter recognition and transcription preinitiation complex assembly 13 2 Murakami Architecture of an RNA polymerase II transcription pre-initiation complex 13 3 Kadonaga The TCT core promoter motif functions with a specialized transcription system 13 4 Werner Progression of archaeal RNA polymerase through the transcription cycle 13 5 Ebright Structural basis of transcription initiation 13 6 Kaplan RNA Polymerase II catalytic activity shapes transcription start site utilization globally in 13 Saccharomyces cerevisiae 7 Levens The interplay of transcription, DNA conformation and DNA topology across the genome 13 8 Price Myc correlates globally with transcription machinery rather than specific DNA elements 13 Session 2 ACTIVATORS AND CO-ACTIVATORS WEDNESDAY 8/28/2013, 9:00 AM J. Conaway # lname Title Talk Length 9 Workman Myeloid leukemia factor acts in a chaperone complex to regulate transcription factor stability and 13 gene expression 10 Chiang Phosphorylation and partner switch regulate Brd4 gene-specific targeting and pathway selectivity 13 11 Guertin Discrete, estrogen-stimulated H3R26 deimination specifically marks and stabilizes ER binding to 13 enhance gene expression 12 Berk Adenovirus e1a reveals a novel function of p300 in transcriptional repression and condensation of 13 higher order chromatin structure 13 Roeder Mechanistic studies of the cooperative functions of transcriptional coactivators on chromatin 13 templates 14 Näär Therapeutic targeting of a transcription factor interface in Mediator 13 15 Fong Novel role of coactivators in stem cell pluripotency 13 16 Hahn Mechanism and specificity of transcription activation domains 13 Session 3 POSTER SESSION I WEDNESDAY 8/28/2013, 2:00 PM # lname Title Talk Length 17 Abdus-Saboor Evidence for a cell fate refinement mechanism in olfactory sensory neurons Page 1 of 13 18 Alpern The TFIID subunit TAF4 is essential for post-natal hepatocyte development by regulating preinitiation complex formation, RNA polymerase II pausing, and promoting HNF4α occupancy of functional sites 19 Ansari Erasing the CTD code—New mechanisms by which Ssu72 regulates Pol II 20 Aristizabal High-throughput genetic and gene expression analysis of the RNAPII-CTD reveals unexpected connections to SRB10/CDK8 21 Arnaudo How do protein complexes recognize the nucleosome? A case study 22 Bahat DTIE is a novel core promoter element that directs start site selection in cancer-associated TATA-less genes 23 Bai Stochastic expression and epigenetic memory at the yeast HO promoter 24 Bai The function of transcription elongation in hematopoietic stem cell development 25 Banyai Cell cycle dependent transcription control by the CDK8 module of the Mediator complex 26 Bartholomeeusen CDK12 mediated regulation of inducible FOS gene expression includes proper 3' processing 27 Baumgart Establishing promotor-proximal paused RNAPII complexes and CDK9-dependent pause-release in vitro 28 Bayles ELL2 regulates Igh mRNA processing and plasma cell development 29 Bernstein H2A histone variants in development and disease 30 Biggin System wide analyses have underestimated transcriptional importance in animals 31 Björklund Mediator subunits control developmental programs and specific metabolic pathways 32 Blombach A TFIIEα/C34 hybrid-like transcription factor in archaea 33 Bonasio Interactions between JARID2 and noncoding RNAs regulate PRC2 recruitment to chromatin 34 Campos Chaperoning histone H3.1 during DNA replication 35 Carlsten Mediators role in centromeric RNA-processing 36 Cecere Positive regulation of Pol II transcription by CSR-1 RNAi pathway in C. elegans 37 Cevher Reconstitution of functional core human Mediator complex 38 Chaubal Regulation and post-translational modifications of SIN3 isoforms 39 Chavez The prefoldin complex regulates chromatin dynamics during transcription elongation 40 Chen Dynamic chromatin organization during embryonic development 41 Chen Site-specific mapping of the protein interaction network within the RNA polymerase III initiation complex 42 Chen AML1-ETO activates transcription through recruitment of demethylase JMJD1C in acute myeloid luekemic cells 43 Chen Using yeast Sen1 to assess the molecular defects of disease mutations in the Senataxin gene that cause motor neuron degeneration Page 2 of 13 44 Cheng Role of H3K4me1 in regulation of facultative heterochromatin and inducible genes 45 Chiu Exploring the role of the U1-PAS axis in regulating divergent transcription 46 Chymkowitch SUMO positively regulates tDNA expression in Saccharomyces cerevisiae 47 Ciesiolka Proteomic analysis of human chromatin-associated RNA polymerase I and accessory factors in elongation of transcription 48 Copur Genetic analysis of histone PTMs in Drosophila 49 Cruz The role of TFIIH subunits in mitosis and cell differentiation during Drosophila melanogaster development 50 Davis The NEAT1 and MALAT1 non-coding RNAs localize to active chromatin 51 Davis The SCF Fbw7 ubiquitin ligase degrades Med13 and Med13L and regulates Cdk8 association with Mediator 52 Devys Yeast and human SAGA acetyltransferase and deubiquitinase activities shape all the transcribed regions of the genome 53 Dikstein Transcription initiation and elongation from a traffic perspective—Traffic control, traffic jams and accidents 54 Donovan FOXO-dependent transcriptional activation of Hsp70 in response to oxidative stress 55 Dronamraju A cell cycle regulated circuit comprising Spt6, Ctk1 and PAF regulates Pol II CTD phosphorylation and transcription elongation 56 Duttke Accurate transcription of RNA polymerase II promoters by RNA polymerase III in vitro 57 Eckersley-Maslin Random monoallelic gene expression upon ESC differentiation 58 Eichinger Disengaging the Smc3/kleisin interface releases cohesin from Drosophila chromosomes during interphase and mitosis 59 Engel Structure-function analysis of RNA polymerase I transcription 60 Esnault Genomic analysis of the actin-regulated MRTF transcriptional response 61 Fernandez Interplay between CTD O-GlcNAcylation and dephosphorylation dependent on PP1α and OGT 62 Fisher-Aylor Genome-wide long distance interaction mapping of RNA Pol2 and the myogenin transcription factor by ChIA-PET—Implications for local “transcription factories” 63 Floer Changes in chromatin architecture upon induction of three pro-inflammatory genes in primary mouse macrophages 64 Fordyce Hac1, a basic leucine zipper transcription factor, recognizes multiple DNA target sites via distinct conformations 65 Fredsøe The importance of topoisomerases for chromatin regulated genes 66 Friedman Chromatin in action—Dynamic view of histone modifications during yeast stress response 67 Fuda GAGA Factor regulation of promoter-proximal pausing of RNA polymerase II in Drosophila Page 3 of 13 68 Fujii Identification and characterization of promoter-bound molecules involved in transcriptional regulation by insertional chromatin immunoprecipitation (iChIP) 69 Fukasawa Mediator CDK subunits have roles in regulating developmental genes cooperatively with chromatin regulators during differentiation 70 Gaarenstroom Mechanism of TGF-β/Smad-mediated transcription on chromatin 71 Gadad Annotation and functional characterization of long noncoding RNAs in breast cancer cells 72 Galbraith Role of the Mediator subunit CDK8 in transcription elongation 73 Gao Role of AUTS2 (Autism Susceptibility Candidate 2), as part of a novel Polycomb Repressive Complex 1 (PRC1), in epigenetic regulation of gene transcription and neuronal functions 74 Ghavi-Helm A temporal view of three-dimensional enhancer interactions during embryonic development 75 Gibson NAD+ analog-sensitive mutants of PARP-1—A new approach for studying PARylation 76 Gokey The role of transcription factors in regulating Pol II pausing 77 Govind Histone deacetylases and Pol II CTD kinases promote Spt6 recruitment to the coding regions 78 Gray An RNA-Seq snapshot reveals genome-wide, in vivo mRNA dynamics from unperturbed cells at steady-state 79 Greifenberg Brd4 activates P-TEFb for RNA Polymerase II CTD Phosphorylation 80 Gruenberg Position and function of initiation factors in the preinitiation and open complex 81 Guglielmi Gene specific transcriptional mechanisms at the histone gene cluster revealed by single cell imaging 82 Guha DNA double stranded breaks enhance the transcription of AIRE target genes 83 Gutierrez The yeast HMG proteins Nhp6A/B and Hmo1 influence SWI/SNF action in vitro and in vivo 84 Haldar BET bromodomains mediate transcriptional pause release in heart failure 85 Hamidian Transcriptional regulation of HIF2A through IGFII mediated PI 3-kinase signalling in neuroblastoma 86 Han Molecular architecture of SAGA revealed by chemical crosslinking and mass spectrometry 87 He Structural visualization of key steps in human transcription initiation 88 Henriques Stable pausing by RNA polymerase II provides a platform for integrating regulatory signals 89 Hermand Signalling through the RNA polymerase II CTD induces gametogenesis by gene-specific chromatin remodeling 90 Herrera-Solorio Processing the amino terminus of histone H3 of Plasmodium falciparum, a new epigenetic mechanism 91 Herzel Analysis of nascent RNA processing in S. pombe by deep sequencing Page 4 of 13 92 Howe Conformation of the histone H3 tail at proline 16 is influenced by residues at position 14 and regulates both H3K4me3 and partitioning of gene expression during