Scaffolding Mechanisms Supporting Fast Neurotransmitter Release at the Presynaptic Active Zone The Harvard community has made this article openly available. Please share how this access benefits you. Your story matters Citation Held, Richard Guy. 2018. Scaffolding Mechanisms Supporting Fast Neurotransmitter Release at the Presynaptic Active Zone. Doctoral dissertation, Harvard University, Graduate School of Arts & Sciences. Citable link http://nrs.harvard.edu/urn-3:HUL.InstRepos:41121214 Terms of Use This article was downloaded from Harvard University’s DASH repository, and is made available under the terms and conditions applicable to Other Posted Material, as set forth at http:// nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of- use#LAA ! ! ! ! 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bstract……………………………………………………………………………………………….…KKK)))! Table of contents………………………………………………………………………………………K…v! Acknowledgments………………………………………………………………………………………..vi! D;7@<R9!YBRL!U*32&(0#3ion………………….……………………………..…………KK………KKKK….1! D;7@<R9!<PYL!RVM"!#&*32&'/!3.-!1&&'!&%!2-$()'4!2-'-$/$8'-!5-/)#'-/!$3!-N#)3$3&24!/4*$1/-/! 3.2&0+.!)3/!BX3-2,)*$'!#&)'-(Xcoil domains……………………...………………………………..….KHZ! D;7@<R9!<;9RRL!T0/)&*!#&,1-3-*3!/4*$13)#!5-/)#'-/!1-2/)/3/!01&*!$#3)5-!6&*-!()/2013)&*! $*(!'&//!&%!5-/)#'-!(&#Qing……………………………………………………………………..….KK…[G! D;7@<R9!TYC9L!9-(0*($*3!/#$%%&'()*+!,-#.$*)/,/!3$2+-3!D$WF!%$,)'4!5&'3$+-X+$3-(! calcium channels to the presynaptic active zone……………..…………………………………….KI\! D;7@<R9!TUWRL!Discussion and Outlook………….……………………………..…………….….HFG! 7@@RB=U]!YBRL!"011'-,-*3$'!T)+02-/K………KKK………………………………………………KKKH^^! 7@@RB=U]!<PY: Supplemental Figures.………..…………………………………………………162! References …………………………..…………………KK……………………………………………KH_^! ! ! #! ! !'/.,0*+12+3+.$#! ! 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