Table S3: List of genes used to reconstruct metabolic map of bin O “Ca. Sabulitectum silens” Glycolysis (complete) No. Gene(s) Gene product EC no. contig G1 glkA Glucokinase 2.7.1.2 contig00029 G2 pgi Glucose-6-phosphate isomerase 5.3.1.9 contig00016 G3 pfkA 6-phosphofructokinase isozyme 1 2.7.1.11 contig00003 G4 pfp Pyrophosphate--fructose 6-phosphate 1-phosphotransferase 2.7.1.90 contig00009 G5 fba Fructose-bisphosphate aldolase 4.1.2.13 contig00010 G6 tpiA Triosephosphate isomerase 5.3.1.1 contig00009 G7 gap Glyceraldehyde-3-phosphate dehydrogenase 1.2.1.12 contig00009 G8 pgk Phosphoglycerate kinase 2.7.2.3 contig00009 G9 pgk/tpi Bifunctional PGK/TIM 2.7.2.3/5.3.1.1 contig00009 G10 apgM 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 5.4.2.12 contig00048 G11 eno Enolase 4.2.1.11 contig00002 G12 pyk Pyruvate kinase 2.7.1.40 contig00002 G13 ppdK Pyruvate, phosphate dikinase 2.7.9.1 contig00002 G14 pckA Phosphoenolpyruvate carboxykinase [ATP] 4.1.1.49 contig00002 Nonoxidative pentose phosphate pathway (complete) No. Gene(s) Gene product EC no. contig P1 tkt Transketolase 2.2.1.1 contig00046 P2 tal Transaldolase 2.2.1.2 contig00046 P3 rpe Ribulose-phosphate 3-epimerase 5.1.3.1 contig00051 P4 rpiB Ribose-5-phosphate isomerase B 5.3.1.- contig00002 P5 prs Ribose-phosphate pyrophosphokinase 2.7.6.1 contig00002 P6 deoB Phosphopentomutase 5.4.2.7 contig00002 P7 deoC Deoxyribose-phosphate aldolase 4.1.2.4 contig00046 Starch and sucrose metabolism (complete) No. Gene(s) Gene product EC no. contig S1 algC Phosphomannomutase/phosphoglucomutase 5.4.2.2 contig00016 S2 glgC Glucose-1-phosphate adenylyltransferase 2.7.7.27 contig00025 S3 rmlA Glucose-1-phosphate thymidylyltransferase 2.7.7.24 contig00003 S4 glgA1 Glycogen synthase 1 2.4.1.21 contig00002 S5 glgB 1,4-alpha-glucan branching enzyme GlgB 2.4.1.18 contig00079 S6 malQ 4-alpha-glucanotransferase 2.4.1.25 contig00013 Glycosyl hydrolases/transferases No. Gene(s) Gene product EC no. contig GH1 Glycosyl hydrolase family 85 3.2.1.96 contig00013 GH2 Cellulase (glycosyl hydrolase family 5) 3.2.1.-. contig00002 GH3 Glycosyl hydrolase family 94 2.4.1.-. contig00016 GT1 Glycosyltransferase family 9 (heptosyltransferase) 2.4.1.-. contig00084 GT2 Glycosyl transferases group 1 2.4.-.-. contig00003 GT3 Glycosyl transferase family 2 2.4.-.-. contig00081 Fermentation No. Gene(s) Gene product EC no. contig F1 nifJ Pyruvate-flavodoxin oxidoreductase 1.2.7.1 contig00048 F2 pta Phosphate acetyltransferase 2.3.1.8 contig00013 F3 ackA Acetate kinase 2.7.2.1 contig00009 F4 Aldehyde ferredoxin oxidoreductase, domains 2 %26 3 1.2.7.5 contig00010 F5 aldHT Aldehyde dehydrogenase, thermostable 1.2.1.5 contig00002 F6 adhT Alcohol dehydrogenase 1.1.1.1 contig00016 F7 buk2 putative butyrate kinase 2 2.7.2.7 contig00042 F8 phosphate butyryltransferase 2.3.1.19 contig00002 F9 gdhA NAD(P)-specific glutamate dehydrogenase 1.4.1.3 contig00002 F10 korA 2-oxoglutarate oxidoreductase subunit KorA 1.2.7.3 contig00003 F10 korB 2-oxoglutarate oxidoreductase subunit KorB 1.2.7.3 contig00003 F11 sucD Succinyl-CoA ligase [ADP-forming] subunit alpha 6.2.1.5 contig00013 F12 gltD Glutamate synthase [NADPH] small chain 1.4.7.1 contig00002 F13 NAD-dependent malic enzyme 1.1.1.40 contig00009 F14 fumarate hydratase 4.2.1.2 contig00009 F15 citE Citrate lyase subunit beta 4.1.3.6 contig00002 F16 acsA Acetyl-coenzyme A synthetase 6.2.1.1 contig00112 F17 scpA Methylmalonyl-CoA mutase 5.4.99.2 contig00002 F18 Methylmalonyl-CoA carboxyltransferase 12S subunit 2.1.3.1 contig00002 MEP/DOXP pathway (complete) No. Gene(s) Gene product EC no. contig I1 dxs 1-deoxy-D-xylulose-5-phosphate synthase 2.2.1.7 contig00010 I2 dxr 1-deoxy-D-xylulose 5-phosphate reductoisomerase 1.1.1.267 contig00046 I3 ispD 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 2.7.7.60 contig00002 I4 ispE 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase 2.7.1.148 contig00003 I5 ispF 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase 4.6.1.12 contig00002 I6 ispG 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase 1.17.7.1 contig00013 I7 ispH 4-hydroxy-3-methylbut-2-enyl diphosphate reductase 1.17.1.2 contig00010 I8 ispA Farnesyl diphosphate synthase 2.5.1.1 contig00009 Nicotidamide synthesis No. Gene(s) Gene product EC no. contig C1 nadB L-aspartate oxidase 1.4.3.16 contig00016 C2 nadA Quinolinate synthase A 2.5.1.72 contig00048 C3 nadC Nicotinate-nucleotide pyrophosphorylase [carboxylating] 2.4.2.19 contig00082 C4 nadD Nicotinate-nucleotide adenylyltransferase 2.7.7.18 contig00076 C5 nadE NH(3)-dependent NAD(+) synthetase 6.3.1.5 contig00009 Biotin synthesis No. Gene(s) Gene product EC no. contig C6 bioA Adenosylmethionine-8-amino-7-oxononanoate aminotransferase 2.6.1.62 contig00029 C7 bioB Biotin synthase 2.8.1.6 contig00029 C8 bioC Malonyl-[acyl-carrier protein] O-methyltransferase 2.1.1.197 contig00029 C9 bioD1 ATP-dependent dethiobiotin synthetase BioD 1 6.3.3.3 contig00029 C10 bioF 8-amino-7-oxononanoate synthase 2 2.3.1.47 contig00029 Thiamine synthesis No. Gene(s) Gene product EC no. contig C11 thiC Phosphomethylpyrimidine synthase 4.1.99.17 contig00082 C12 thiE Thiamine-phosphate synthase 2.5.1.3 contig00002 C13 thiH 2-iminoacetate synthase 4.1.99.19 contig00002 C14 thiM Hydroxyethylthiazole kinase 2.7.1.50 contig00002 C15 thiD Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase 2.7.4.7 contig00002 C16 thiL Thiamine-monophosphate kinase 2.7.4.16 contig00002 Riboflavin synthesis No. Gene(s) Gene product EC no. contig C17 ribBA Riboflavin biosynthesis protein RibBA 4.1.99.12/3.5.4.25 contig00002 C18 ribH 6,7-dimethyl-8-ribityllumazine synthase 2.5.1.78 contig00002 C19 ribE Riboflavin synthase 2.5.1.9 contig00002 C20 ribD Riboflavin biosynthesis protein RibD 3.5.4.26/1.1.1.193 contig00002 Nuo-like NADH Oxidoreductase No. Gene(s) Gene product EC no. contig N1 nuoE NADH-quinone oxidoreductase subunit E 1.6.5.11 contig00002 N1 nuoC1 NADH-quinone oxidoreductase subunit C 1 1.6.5.11 contig00002 N1 nuoD NADH-quinone oxidoreductase subunit D 1.6.5.11 contig00002 N1 nuoH NADH-quinone oxidoreductase subunit H 1.6.5.11 contig00002 N1 nuoI NADH-quinone oxidoreductase subunit I 1.6.5.11 contig00002 N1 ndhK NAD(P)H-quinone oxidoreductase subunit K 1.6.5.11 contig00002 N1 nox NADH dehydrogenase 1.6.99.3 contig00002 Hyd-like (Fe) Hydrogenase No. Gene(s) Gene product EC no. contig H1 hydA Periplasmic [Fe] hydrogenase large subunit 1.12.7.2 contig00084 H1 hndC NADP-reducing hydrogenase subunit HndC 1.12.1.3 contig00084 H1 hndD NADP-reducing hydrogenase subunit HndD 1.12.1.3 contig00084 H1 nqo2 NADH-quinone oxidoreductase subunit 2 1.6.5.11 contig00084 H1 Iron hydrogenase 1 1.12.7.2 contig00084 Hnd-like Hydrogenase No. Gene(s) Gene product EC no. contig H2 hndA NADP-reducing hydrogenase subunit HndA 1.-.-.- contig00010 H2 hndC NADP-reducing hydrogenase subunit HndC 1.-.-.- contig00010 H2 hndD NADP-reducing hydrogenase subunit HndD 1.-.-.- contig00010 Hox-like Hydrogenase No. Gene(s) Gene product EC no. contig H3 hoxE bidirectional hydrogenase complex protein HoxE 1.12.1.2 contig00010 H3 hoxF NAD-reducing hydrogenase HoxS subunit alpha 1.12.1.2 contig00048 H3 hoxH NAD-reducing hydrogenase HoxS subunit beta 1.12.1.2 contig00048 H3 hoxU NAD-reducing hydrogenase HoxS subunit gamma 1.12.1.2 contig00048 H3 hoxY NAD-reducing hydrogenase HoxS subunit delta 1.12.1.2 contig00048 Hyp-like Hydrogenase chaperone No. Gene(s) Gene product EC no. contig H4 hypA hydrogenase nickel incorporation protein HypA - contig00003 H4 hypB Hydrogenase/urease nickel incorporation protein HypB - contig00003 H4 hypD Hydrogenase isoenzymes formation protein HypD - contig00048 H4 hypE Hydrogenase expression/formation protein HypE - contig00048 H4 hypF Carbamoyltransferase HypF - contig00048 V-type proton/sodium ATPase No. Gene(s) Gene product EC no. contig V1 ntpA V-type sodium ATPase catalytic subunit A 3.6.3.- contig00013 V1 ntpB V-type sodium ATPase subunit B 3.6.3.- contig00013 V1 ntpK V-type sodium ATPase subunit K 3.6.3.- contig00013 V1 ntpD V-type sodium ATPase subunit D 3.6.3.- contig00013 V1 ntpG V-type sodium ATPase subunit G 3.6.3.- contig00013 V1 atpC V-type ATP synthase subunit C 3.6.3.- contig00013 V1 atpE V-type proton ATPase subunit E 3.6.3.- contig00013 V1 V-type ATP synthase subunit I 3.6.3.- contig00013 Electron transport complex No. Gene(s) Gene product EC no. contig E1 rnfA Electron transport complex protein RnfA 1.18.1.3 contig00055 E1 rnfB Electron transport complex protein RnfB - contig00055 E1 rnfC Electron transport complex protein RnfC - contig00055 E1 rnfD Electron transport complex protein RnfD - contig00055 E1 rnfE Electron transport complex protein RnfE - contig00055 E1 rnfG Electron transport complex protein RnfG - contig00055 Phosphate uptake No. Gene(s) Gene product EC no. contig T1 pstA Phosphate transport system permease protein PstA - contig00003 T1 pstB Phosphate import ATP-binding protein PstB - contig00003 T1 pstB3 Phosphate import ATP-binding protein PstB 3 - contig00003 T1 pstC Phosphate transport system permease protein PstC - contig00003 Na+/Pi-cotransporter contig00003 Potassium uptake No. Gene(s) Gene product EC no. contig T2 trkA Trk system potassium uptake protein TrkA - contig00002 T2 trkG Trk system potassium uptake protein TrkG - contig00002 T2 trkH Trk system potassium uptake protein TrkH - contig00002 T3 ktrA Ktr system potassium uptake protein A - contig00010 T3 ktrB Ktr system potassium uptake protein B - contig00010 Iron uptake No.