Electronic Supplementary Material (ESI) for Food & Function. This journal is © The Royal Society of ChemistryPlease do2021 not adjust margins Journal Name ARTICLE Table S1. Classification criteria for type of response according to VAS-IBS. Subtype Responders Non-responders IBS-C Change in "Constipation" value ≥ 1cm IBS-D Change in "Diarrhoea" value ≥ 1cm Participants who do not meet conditions by subtype, no change in main symptom. IBS-M Change in "Constipation" and "diarrhoea" values ≥ 1cm IBS with constipation (IBS-C), IBS with diarrhea (IBS-D), and IBS with mixed symptoms of both constipation and diarrhea (IBS-M) This journal is © The Royal Society of Chemistry 20xx J. Name., 2013, 00, 1-3 | 1 Please do not adjust margins Please do not adjust margins ARTICLE Journal Name Table S2. Bacterial genus with major contribution to PC1. Phylum Class Order Family Genus Firmicutes Clostridia Clostridiales Lachnospiraceae Eubacterium Actinobacteria Coriobacteria Coriobacteriales Eggerthellaceae - Actinobacteria Actinobacteridae Bifidobacteriales Bifidobacteriaceae Scardovia Firmicutes Clostridia Clostridiales XIII Eubacterium Actinobacteria Coriobacteriia Coriobacteriales Eggerthellaceae Adlercreutzia 2 | J. Name., 2020, 00, 1-3 This journal is © The Royal Society of Chemistry 20xx Please do not adjust margins Please do not adjust margins Journal Name ARTICLE Supplementary Figure 1. Alpha and Beta diversity in responsive (R) and non-responsive (NR) subjects before (B) and after (A) low-FODMAP diet consumption. A) Chao1, ACE, Inverse Simpson, and Fisher metrics. B) Bray Curtis distances. C) Sorensen Dissimilarity. Alpha diversity pairwise comparisons using the Wilcoxon rank-sum test. p <0.05). Beta diversity permutational ANOVA (PERMANOVA) analysis. p <0.05) This journal is © The Royal Society of Chemistry 20xx J. Name., 2013, 00, 1-3 | 3 Please do not adjust margins.