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Table S1. Classification criteria for type of response according to VAS-IBS. Subtype Responders Non-responders IBS-C Change in "Constipation" value ≥ 1cm IBS-D Change in "Diarrhoea" value ≥ 1cm Participants who do not meet conditions by subtype, no change in main symptom. IBS-M Change in "Constipation" and "diarrhoea" values ≥ 1cm IBS with constipation (IBS-C), IBS with diarrhea (IBS-D), and IBS with mixed symptoms of both constipation and diarrhea (IBS-M)

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Table S2. Bacterial genus with major contribution to PC1. Phylum Class Order Family Genus Clostridia Clostridiales Lachnospiraceae Eubacterium Coriobacteria - Actinobacteria Actinobacteridae Bifidobacteriales Bifidobacteriaceae Scardovia Firmicutes Clostridia Clostridiales XIII Eubacterium Actinobacteria Coriobacteriales Eggerthellaceae Adlercreutzia

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Supplementary Figure 1. Alpha and Beta diversity in responsive (R) and non-responsive (NR) subjects before (B) and after (A) low-FODMAP diet consumption. A) Chao1, ACE, Inverse Simpson, and Fisher metrics. B) Bray Curtis distances. C) Sorensen Dissimilarity. Alpha diversity pairwise comparisons using the Wilcoxon rank-sum test. p <0.05). Beta diversity permutational ANOVA (PERMANOVA) analysis. p <0.05)

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