Table of Contents The 37th Steenbock Symposium Page 1 General Information e Future of Chemical Biology 2 Support Staff 3 About Harry Steenbock e symposium will bring together leading 3 Area Map scientists in the eld of chemical biology to discuss their most recent research activities. 4 - 5 Schedule 6 Sponsors Presentations will cover topics including: 7 Symposium Organizer • Natural Products 8 - 9 Speaker Index • Small Molecule Modulators 9 Chair Index • Post-translational Modification 10 - 57 Speaker Bios & Abstracts • Imaging 58 - 62 Poster Presenter Index • Protein Quality Control 64 - 149 Poster Abstracts • Chemistry for Chemical Biology 151- 155 Attendees General Information Name Badges Symposium participants are requested to wear the name badge during all conference activities. Registration Pick up conference materials on Thursday, June 5th, 5:00-6:50 pm Ebling Symposium Center Lobby, Microbial Sciences Building, 1550 Linden Drive. Opening Reception Thursday, June 5th, 5:00-6:50 pm. Welcoming and Keynote speakers starting at 7:00 pm. Ebling Symposium Center Lobby, Microbial Sciences Building, 1550 Linden Drive. Scientific Talks All talks will be in the Ebling Symposium Center, Microbial Sciences Building, 1550 Linden Drive. Talks begin at 8:55 am on Friday and conclude by 5:00 pm Saturday. Breakfast & Lunches Friday and Saturday. Please wear your name badge. Registration fee includes breakfasts, breaks and lunches. See schedule for locations. Poster Sessions Poster Session 1: Friday 12:30-1:40 pm, HF DeLuca Biochemistry Laboratories, 433 Babcock Drive Posters can go up starting at 7:00 am Friday. To be taken down at the end of the poster session. Poster Session 2: Saturday 1:30-2:40 pm, HF DeLuca Biochemical Sciences Building, 440 Henry Mall Posters can go up starting at 8:30 am Saturday. To be taken down at the end of the poster session. Boards are numbered and reserved. Please see the reservation list in this booklet and near the boards. Banquet Friday, June 6th, 6:30-9:45 pm,Wisconsin Institutes for Discovery, 330 North Orchard Street Banquet included in the registration fee. The Future of Chemical Biology 1 The Future of Chemical Biology 37th Steenbock Symposium University of Wisconsin – Madison Symposium Organizer Laura L. Kiessling Departments of Biochemistry & Chemistry University of Wisconsin – Madison Symposium Coordinators Kris Turkow Department of Chemistry University of Wisconsin – Madison Leah Leighty CALS Conference Services University of Wisconsin – Madison Robin Davies & Laura Vanderploeg Symposium Web Site, Abstract Book & Illustrations 2 37th Steenbock Symposium June 5th – 7th, 2014 Harry Steenbock 1886–1967 A distinguished Professor of Biochemistry at the University of Wisconsin-Madison, Professor Steenbock's interests and contributions spanned many areas of nutrition and biochemistry. Of special note is his discovery of the conversion of carotenes to vitamin A, the production of vitamin D by ultraviolet irradiation, and his central participation in demonstrating that copper and iron cure nutritional anemia. The production of vitamin D by ultraviolet irradiation virtually abolished rickets, a widespread and debilitating disease. Professor Steenbock patented the irradiation process for producing vitamin D and conceived the idea of the Wisconsin Alumni Research Foundation (WARF), a nonprofit foundation which invests the proceeds from patents for support of research in the natural sciences at the University of Wisconsin-Madison. Professor Steenbock assigned his valuable patents to the Foundation, and revenue from them has provided uniquely flexible resources for fostering sustained scientific development on the Madison campus. To honor Professor Steenbock, the Steenbock Endowment sponsors a symposium at the University of Wisconsin-Madison. Area Map JOHNSON ST GORHAM ST N. PINCK NEY GILMAN ST LAKE S. BLAIR MENDOTA S. BUTLER ST LAK 1. Microbial Sciences Building ESI DE B WIS (Site of Symposium) IKE / HIKING PA TH N. CARROLL AVE 2. H.F. DeLuca Biochemistry Laboratories (Site of Poster Session 1) 6 DAY TON ST 3. H.F. DeLuca Biochemical Sciences Building 1 LINDEN DR LANGDON ST STATE ST STATE (Site of Poster Session 2) BABCOCK DR CAPITOL 2-3 Lot 20 4. Wisconsin Institutes for Discovery MALL < > HENRY 7 (Site of the Banquet) S. PINCKNEY UNIVERSITY AVE 4 HENRY ST 5. Union South 6. Memorial Union JOHNSON ST Lot 17 5 BROOM 7. Lake St. & Frances St. Parking Ramps DAYTON ST S. CARROLL MARTIN LUTHER KING BLVD CAMP BASSETT JOHN NOLEN DR. RANDALL LAKE ST PARK ST MARION ST FRANCES ST CHARTER ST ORCHARD ST RANDALL AVE WILSON ST W. WASHINGTON AVE REGENT ST MONROE ST LAKE MONONA The Future of Chemical Biology 3 Symposium Schedule Thursday, June 5th, 2014 Registration & Hors d'oeuvres 5:00 - 6:50 PM Ebling Symposium Center Lobby, Microbial Sciences Building, 1550 Linden Drive Welcome & Opening Remarks – Laura Kiessling 7:00 PM Ebling Symposium Center 7:15 - 8:15 PM Keynote – Dennis Dougherty ** All talks will be in the Ebling Symposium Center ** Microbial Sciences Building, 1550 Linden Drive Friday, June 6th, 2014 8:30 - 8:50 AM Breakfast – Ebling Lobby 8:55 - 10:30 AM Session I • Natural Products 8:55 AM Chair: Helen Blackwell 9:00 AM Rebecca Butcher 9:30 AM Yi Tang 10:00 AM Frank Schroeder 10:30 - 10:50 AM Break – Ebling Lobby 10:55 - 12:30 PM Session II • Small Molecule Modulators 10:55 AM Chair: Dehua Pei 11:00 AM Nathanael Gray 11:30 AM Dustin Maly 12:00 PM Jack Taunton Lunch & Poster Session 1 12:30-1:40 PM HF DeLuca Biochemistry Laboratories, 433 Babcock Drive Posters can go up starting at 7:00 am Friday. To be taken down at the end of the poster session. 1:55 - 4:00 PM Session III • Post-translational Modification 1:55 PM Chair: Martha Oakley 2:00 PM Minkui Luo 2:30 PM Chuan He 3:00 PM Eranthie Weerapana 3:30 PM Mark Distefano 4:00 - 4:20 Break – Ebling Lobby 4:25 - 5:30 PM Session IV• Imaging 4:25 PM Chair: Brett Van Veller 4:30 PM Luke Lavis 5:00 PM Christopher Chang 6:30 PM Reception – Wisconsin Institutes for Discovery, 330 North Orchard Street 7:30- 9:45 PM Dinner Banquet – Wisconsin Institutes for Discovery, 330 North Orchard Street 4 37th Steenbock Symposium June 5th – 7th, 2014 Symposium Schedule Saturday, June 7th, 2014 8:30 - 8:50 AM Breakfast – Ebling Lobby 8:55 - 11:00 AM Session V • Protein Quality Control 8:55 AM Chair: Eric Strieter 9:00 AM Jason Gestwicki 9:30 AM Alexander Statsyuk 10:00 AM Peter Chien 10:30 AM Sachdev Siddhu 11:00 - 11:20 AM Break – Ebling Lobby 11:25 - 1:30 PM Session VI • Chemistry for Chemical Biology 11:25 AM Chair: Jiaoyang Jiang 11:30 AM Ratmir Derda 12:00 PM Nicola Pohl 12:30 PM Geert-Jan Boons 1:00 PM David Spiegel Lunch & Poster Session 2 1:30-2:40 PM HF DeLuca Biochemical Sciences Building, 440 Henry Mall Posters can go up starting at 8:30 am Saturday. To be taken down at the end of the poster session. 2:55 - 4:30 PM Session VII• Small Molecule Modulators II 2:55 PM Chair: Eric Underbakke 3:00 PM Douglas Weibel 3:30 PM Paul Thompson 4:00 PM Hening Lin 4:30 PM Closing Remarks – Laura Kiessling 1 1. Microbial Sciences Building N Site of Symposium • 1550 Linden Drive Linden Drive Henry Mall 2. H.F. DeLuca Biochemistry Laboratories Site of Poster Session 1 • 433 Babcock Drive 4 Henry Mall 2 3 3. H.F. DeLuca Biochemical Sciences Building 3 Site of Poster Session 2 • 440 Henry Mall 2 Babcock Drive Campus Drive 4. Wisconsin Institutes for Discovery Site of the Banquet • 330 North Orchard University Avenue 4 North Orchard St North Randall Ave The Future of Chemical Biology 5 Symposium Sponsors The Steenbock Endowment Department of Biochemistry University of Wisconsin – Madison 6 37th Steenbock Symposium June 5th – 7th, 2014 Symposium Organizer Laura L. Kiessling Laura L. Kiessling was born in Milwaukee, WI. She received her B.S. in Chemistry from MIT and her Ph.D. in Chemistry from Yale University. After postdoctoral training in Chemical Biology at the California Institute of Technology, she returned to Wisconsin to begin her independent career at the University of Wisconsin-Madison in 1991. She currently is a Steenbock Professor of Chemistry and the Laurens Anderson Professor of Biochemistry. She also serves as the Director of the Keck Center for Chemical Genomics, the Program Director for the Chemical Biological Interface Training Program, and the Editor-In-Chief of ACS Chemical Biology. Her interdisciplinary research interests focus on elucidating and exploiting the mechanisms of cell surface recognition processes, including those that involve of protein–saccharide recognition and oligosaccharide function. Another major interest of her group is multivalency and its role in recognition and signal transduction. Her research combines tools from organic synthesis, polymer chemistry, structural biology, microbiology, and molecular and cell biology to address problems in chemical biology. More information on Dr. Kiessling and her research can be found at www.biochem.wisc.edu/faculty/kiessling/lab/. The Future of Chemical Biology 7 Speaker Index Abstract Day Speaker Title of Talk Page Time Functional Glycomics Through Chemical Saturday Geert-Jan Boons 11 Synthesis 12:30 PM Chemical Communication in C. elegans and other Friday Rebecca A. Butcher 13 Nematodes 9:00 AM Molecular Approaches to Mapping and Friday Christopher J. Chang 15 Understanding Inorganic Chemistry in the Brain 5:00 PM Saturday Peter Chien Regulated Protein Degradation in Bacteria 17 10:00 AM Discovery of Ligands from Genetically-encoded Saturday Ratmir Derda 19 Libraries of Synthetic Molecules 11:30 AM Friday Mark Distefano Chemical Biology of Protein Prenylation 21 3:30 PM Chemistry on the Brain: Thursday Dennis A. Dougherty 23 Understanding the Nicotine Receptor 7:15 PM Protein Folding in the Eye: Saturday Jason E.