A REVOLUTION IN THE PHYSIOLOGY OF THE LIVING CELL FUTURE ANNUAL MEETINGS OF by Gilbert N. Ling Orig. Ed. 1992 404 pp. $64.50 THE AMERICAN SOCIETY FOR ISBN 0-89464-398-3 CELL BIOLOGY The essence of a major revolution in cell physiology -the first since the cell was recognized as the basic unit of life a century and a half ago - is presented and altemative theories are discussed in this text. Although the conventional mem- brane-pump theory is still being taught, a new theory of the living cell, called the association-induction hypothesis has been proposed. It has successfullywfthstood 1995 twenty-five years of worldwide testing and has already generated an enhancing DC diagnostic tool ofgreat power, magnetic resonance imaging (MRI).This volume is Washington, intended forteachers, students and researchers ofbiology and medicine. December 9-13 i't...a correct basic theory of cell physiology, besides its great intrinsic value in mankind's search forknowledge aboutourselves and the world we live in, willalso play a crucial role in the ultimate conquest of cancer, AIDS, and other incurable 1996 diseases.'-from the Introduction. ASCB Annual Meeting/ on Cell Biology t When ordering please add t".Ling turns cellphysiology upside down. He Sixth International Congress $5.00 first bookl$1.50 each ad- practicallyredefines the cell. He provides com- San Francisco, California ditional book to cover shipping pellingevidencefor headequacyofhis theoryn December 7-11 charges. Foreign shipping costs evidence that cannot fail to impress even the available upon request. most extreme skeptics....'- Gerald H. Pollock, _ Ph.D., Univ. ofWashington. Please call or write for our Free Biology catalog 1997 KRIEGER PUBLISHING COMPANY Washington, DC OP. Box 9542 ' Melbourne, FL32902-9542 December 13-17 (407) 724-9542 ' Order Dept. (407) 727-7270 2 \/ ~~~FAX(407)951-3671 + Statement of Ownership, Management and Circulation (Required by 39 U. S C. 3685) 1A lleo P,lbl--tro I PUBLICATION NO Grl .'AlP rilin,g M1,,lcI. lar Aiolo9y ,f th Ce.l'r,1l 0 Ii| 4 1 11/1/94 3Feeni Iss- 3A N. ra P,t,,,Ih.d 3B | ks--e A-1-1llSbctir Pr-c A--nvIa,y Mb6 r Sf3No$10 1 $150 Mo.,t8UIy 12 - iist1 $300 Stud 140 NO MORE RABBIT WRANGLING ASnCI, 965,))l1ockwil1, P6ik^, 111.61111111, ML) 20814-3112 Sam,e_.11~1 a:;_BI t:er_._4...4 2 _ ,11.,__.,II20. .65)0. _ _ _.1 1,. ._ 6 F.11 Nia-re -ld PoiPlt A.rilrq Add-lRres 0r FVA1h,15t. fd-IIrr. -dle M.-,,y Ul Fd,tear iTh.l r .1 .7hJ1 ln^ ASCBII 9461 R88ck6ill) [like, B11tl)S,la, MD 20814-1912 Dla,,vid6 RII1t911t1e8iAS C6B 0 Roc8k16vi Iiko B1t. 151s,la1 M4D 208I14-3992 Ma ag,nq Ed-orlN......i./Jr...IB /rAhfrJS,rt.il,' R5a1 IIha KaAA,ABmaAASiBR, LA1651 Rltykv.i1j1ePiike1Bet.11,21BH2I1--3932BBI ~~~~~~~~~F.....N..... C..p__Fl.gNl.sCrnlMaIlingAdd,... The AmL.Li=:ai SQLcieLy fur .Cclll Tiolocy... ... 9651} RoDckville Pike, Bethesda MD 2s1Si4-3992 41.B.,.......11B d I,I Il 11B11p11*BB,.) ...... Ad'. K r,-,,B.'1111.1. B,.. _11g.,;-..J..,0B1-B_ 1r..M1- .I -i__B_..$M-BBB H OB1..1.. A_..._ T.W Secrre rOf h,tesr rtrr rr A11oilPh."":1 - CC'T1994 F.11 Nnm....C-mpllw. M.i0lng Add,... .1B1 _ 21111,1 14 _____......._.__..._. ANIMAL PHARM SERVICES 9 F B,C BmBleBo-b N.-,p,o 0rg-B 1i,-i-rs.A -BiBBdT.11Aa BtSpIBR.t.s SBBrBB4B12BIBB)B 111: B TheF1dC f-- 8 1.d B IN, .,Id lh. W26622100Mr I ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~. * r l-n - r n ,c. H.. N., Ch.ar,g,d O u r n g (:l1-,rg.J D,-rarg 1/f I-hrJnr0eI ......he...J "PW-evptafiterrJ P.B..B PrBdB, 2 11,Pr-d,rBdIB1 BBr1-.BIBIB1IBBIBB12 M- 11Ih- - - *n EXl rulad N-llr. 1,f (:tllAerl.g. Ne C..,i.. E.r.h I-.v Ouring Acr-I N.D C.pi.. Single I.... We will supply you with high quality polyclonal -Weeri4e.r, el,f ,11r eeel 17 Moral.hs PLbh.hhed N--es 10 Filing D... A ta N,, P-,le(N,R.rPrl Vr 2&fi2 210{} antibodies. We use specific pathogen free BIPard *nd:o,rRqeed.. C,.d aio 1. SleI.. ue -.1er*n 1er1-eedor *nd onesle .. _ _.___ ._ . ____ _ _ 2 NI. Sb ,rP,,-n animals. Rabbits, guinea pigs and goats are 1324 $409 C T.Wa Ps,d -dWe, R.,,...(.d lesto immediately available. Other animals by special IS1u90J1-11 111121 13 24 1409 O. T".. Dwlsrb.t-n by M.ti1. C-4,ie or Olh., hl-an order. Best Prices. Best Service. Best Animals. B.116B.1C1111p,I-V.11.1dO1b., F-ree C.p-B 4 .3_ 158 E rOl1w 1,D 8rb-- Bn d101 1777 1 559 . For complete pricing and availability call: F. C.p..s NolDsrbue Off .1P.o.ve- - -d111BIBB-d.1 ., R885 5_4$ 2. Relurr f-o N.,- Ag.nts 0} 1 B 6I G '.TOTAL S111B1 F. Fl -d2 -1I118$Bld9-1- p1-1B B1r4A 2662 21.00 11 5~~~~~~~~~~~~~~~~-g.--Jr-rd T,tI. ,f Fd,M,t. Pu,ble"r B...... M@ne-go- O ne cBrtify that the statemIiBts made bY B- 7 BB g110BB USDA-NIH assurances "GLP' available me abo,ve aB corBect Bnd complete ,4112ZJ9%raB.I'-11. 9"wY-t PS FB.- 3526.____199BBB_.....~~~~~~... /Srr r. Molecular Biology * 0* ¢* O* ** 0 4p 0 of the Cell 0f* .t 4 Editor-in-Chief Editor David Botstein Keith R. Yamamoto Stanford University School of Medicine University of California, San Francisco Associate Editors J. Michael Bishop Carl-Henrik Heldin Erkki Ruoslahti University of California, San Francisco Ludwig Institute for Cancer Research, La Jolla Cancer Research Foundation Elizabeth H. Blackburn Uppsala, Sweden Gottfned Schatz University of California, San Francisco Kenneth Holmes University of Basel, Switzerland Michael S. Brown Max-Planck-Institut fur Medizinische Randy W. Schekman University of Texas Southwestern Forschung, Heidelberg, Germany University of California, Berkeley Medical Center, Dallas Tim Hunt Joseph Schlessinger Gerald R. Fink ICRF Clare Hall Labs, United Kingdom New York University Medical Center Whitehead Institute, Cambridge Richard Hynes Lucy Shapiro Joseph Gall Massachusetts Institute of Technology, Stanford University School of Medicine Carnegie Institution, Baltimore Cambridge Judith Kimble Timothy A. Springer Mary-Jane Gething University of Wisconsin, Madison Center for Blood Research, Boston University of Texas Southwestern James A. Spudich Medical Center, Dallas Marc W. Kirschner Harvard Medical School, Boston Stanford University School of Medicine Alfred G. Gilman Masatoshi University of Texas Southwestern Timothy J. Mitchison Takeichi Medical Center, Dallas University of California, San Francisco Kyoto University, Japan Roger Y. Tsien Joseph L. Goldstein Elliot Meyerowitz San University of Texas Southwestern California Institute of Technology, Pasadena Universityk of California, Diego Michael H. Medical Center, Dallas Thomas D. Pollard Wigler Guido Guidotti Johns Hopkins University, Baltimore Cold Spring Harbor Laboratory Harvard University, Cambridge Mitsuhiro Yanagida Martin Raff Kyoto University, Japan Leland Hartwell University College London, United Kingdom University of Washington, Seattle Joan Ruderman Harvard Medical School, Boston Essay Editors Art & History Editor Managing Editor Bruce M. Alberts Joseph Gall Rosalba A. Kampman University of California, San Francisco Carnegie Institution, Baltimore The American Society for Cell Biology, Henry R. Bourne Bethesda University of California, San Francisco Editorial Board Ken-Ichi Arai James R. Feramisco Chris Kaiser Gerald M. Rubin William Balch Douglass J. Forbes Mary B. Kennedy Melvin Simon Mary Beckerle Thomas D. Fox Michael Klagsbrun William S. Sly Merton Bemfield Margaret T. Fuller Stuart Komfeld Solomon H. Snyder Michael Berridge Michael M. Gottesman Robert J. Lefkowitz Paul W. Steinberg Melanie Cobb Dan Gottschling Marc Mumby Antti Vaheri Gerald Crabtree Warner Greene Paul Nurse Denisa Wagner Tom Curran Ed Harlow Bjorn Olsen Peter Walter Benoit de Crombrugghe Tony Hunter Suzanne R. Pfeffer Paul M. Wassarman Gregor Eichele Rudolf Jaenisch Steven I. Reed Tadashi Yamamoto Marilyn Farquhar Elizabeth Jones Publication Staff Kevin Flynn Kathryn King Georgia Monitor Edward Newman Editorial Assistant Production Manager Subscriptions Advertising Owned and pubLished by The American Society for Cell Biology, Elizabeth Marincola, Executive Director Cover Sixty years ago the German cytologist E. Heitz introduced the terms heterochromatin and euchromatin to distinguish parts of chromosomes that remain condensed throughout the cell cycle from those that undergo the usual cycle of condensation at mitosis and decondensation during interphase. Studies by Heitz and others on Drosophila were particularly helpful in defining the genetic properties of heterochromatin. The upper left photo on the cover shows a prophase of D. melanogaster in which each chromosome except the Y contains a large block of heterochromatin surrounding the centromere. The Y consists entirely of heterochromatin. Homologous regions of the euchromatin in the two longest chromosomes are paired, a peculiarity of the Diptera. The chromosomes of D. virilis in the upper right photo have a large block of heterochromatin next to the terminal centromere and a distal euchromatic segment. In this metaphase configuration the euchromatin and heterochromatin are both condensed but can be distinguished by the separated sister chromatids in the euchromatin. Genetic analysis of inversions and translocations showed that all but one or two of the known genes were in the euchromatin, and that large parts of the heterochromatin could be deleted without genetic consequences. When the polytene chromosomes were rediscovered in 1933 and compared with their mitotic counterparts, the heterochromatin again stood out as unusual: the banded arms consisted entirely of euchromatin, whereas the heterochromatin from all the chromosomes was drastically reduced in amount and fused into a single region called the chromocenter. As shown for D.