AUTOPHAGY 2016, VOL. 0, NO. 0, 1–215 http://dx.doi.org/10.1080/15548627.2015.1100356 EDITORIAL Guidelines for the use and interpretation of assays for monitoring autophagy (2nd edition) Daniel J Klionsky1766,1771*, Kotb Abdelmohsen851, Akihisa Abe1253, Md Joynal Abedin1784, Hagai Abeliovich428, 5 Abraham Acevedo Arozena800, Hiroaki Adachi1822, Christopher M Adams1690, Peter D Adams58, Khosrow Adeli2010, Peter J Adhihetty1645, Sharon G Adler710, Galila Agam68, Rajesh Agarwal1607, Manish K Aghi1556, Maria Agnello1848, Patrizia Agostinis673, Patricia V Aguilar1987, Julio Aguirre-Ghiso795,797, Edoardo M Airoldi90,425, Slimane Ait-Si-Ali1394, Takahiko Akematsu2039, Emmanuel T Akporiaye1112, Mohamed Al-Rubeai1412, Guillermo M Albaiceta1311, Chris Albanese366, Diego Albani569, Matthew L Albert524, Jesus Aldudo129, Hana Algul€ 1179, Mehrdad Alirezaei1213, 10 Iraide Alloza650,899, Alexandru Almasan207, Maylin Almonte-Becerril531, Emad S Alnemri1228, Covadonga Alonso552, Nihal Altan-Bonnet859, Dario C Altieri1220, Silvia Alvarez1516, Lydia Alvarez-Erviti1413, Sandro Alves108, Giuseppina Amadoro871, Atsuo Amano942, Consuelo Amantini1573, Santiago Ambrosio1477, Ivano Amelio767, Amal O Amer929, Mohamed Amessou2122, Angelika Amon737, Zhenyi An1557, Frank A Anania294, Stig U Andersen6, Usha P Andley2111, Catherine K Andreadi1711, Nathalie Andrieu-Abadie509, Alberto Anel2058, David K Ann59, 15 Shailendra Anoopkumar-Dukie391, Manuela Antonioli843,1911, Hiroshi Aoki1813, Nadezda Apostolova1432, Saveria Aquila1519, Katia Aquilano1900, Koichi Araki295, Eli Arama2131, Agustin Aranda460, Jun Araya599, Alexandre Arcaro1491, Esperanza Arias27, Hirokazu Arimoto1241, Aileen R Ariosa1770, Jane L Armstrong1956, Thierry Arnould1795, Ivica Arsov2153, Katsuhiko Asanuma684, Valerie Askanas1949, Eric Asselin1891, Ryuichiro Atarashi805, Sally S Atherton372, Julie D Atkin724, Laura D Attardi1146, Patrick Auberger1809, Georg Auburger382, Laure Aurelian1748, 20 Riccardo Autelli2021, Laura Avagliano1043,1777, Maria Laura Avantaggiati367, Limor Avrahami1181, Suresh Awale2015, Tiziana Bachetti, Jonathan M Backer29, Dong-Hun Bae1959, Jae-sung Bae686, Ok-Nam Bae412, Soo Han Bae2150, Eric H Baehrecke1750, Seung-Hoon Baek18, Stephen Baghdiguian1386, Agnieszka Bagniewska-Zadworna2, Hua Bai91, Jie Bai676, Xue-Yuan Bai1148, Yannick Bailly895, Kithiganahalli Narayanaswamy Balaji478, Walter Balduini2031, Andrea Ballabio319, Rena Balzan1732, Rajkumar Banerjee241,Gabor Banhegyi1067, Haijun Bao2142, Benoit Barbeau1381, 25 Maria D Barrachina2036, Esther Barreiro472, Bonnie Bartel1011, Alberto Bartolome223, Diane C Bassham558, Maria Teresa Bassi1061, Robert C Bast Jr1290, Alakananda Basu1820, Maria Teresa Batista1597, Henri Batoko1353, Maurizio Battino984, Kyle Bauckman2117, Bradley L Baumgarner1933, K Ulrich Bayer1614, Rupert Beale1572, Jean-Francois¸ Beaulieu1378, George R. Beck Jr49,297, Christoph Becker339, J David Beckham1615, Pierre-AndreB edard 760, Patrick J Bednarski304, Thomas J Begley1150, Christian Behl1437, Christian Behrends768, Georg MN Behrens409, 30 Kevin E Behrns1647, Eloy Bejarano8, Amine Belaid496, Francesca Belleudi1056, Giovanni Benard 503, Guy Berchem716, Daniele Bergamaschi997, Matteo Bergami1419, Ben Berkhout1459, Laura Berliocchi725,Amelie Bernard1770, Monique Bernard1372, Francesca Bernassola1904, Anne Bertolotti802, Amanda S Bess275,Sebastien Besteiro1368, Saverio Bettuzzi1851, Savita Bhalla924, Shalmoli Bhattacharyya987, Sujit K Bhutia849, Caroline Biagosch1174, Michele Wolfe Bianchi527,1396,1399, Martine Biard-Piechaczyk211, Viktor Billes301, Claudia Bincoletto1331, Baris Bingol353, 35 Sara W Bird1143, Marc Bitoun1127, Ivana Bjedov1274, Craig Blackstone854, Lionel Blanc1198, Guillermo A Blanco1515, Heidi Kiil Blomhoff1834, Emilio Boada-Romero1314, Stefan Bockler€ 1483, Marianne Boes1441, Kathleen Boesze-Battaglia1858, Lawrence H Boise289,290, Alessandra Bolino2094, Andrea Boman703, Paolo Bonaldo1845, Luis M Botana1325, Matteo Bordi908, Jurgen€ Bosch616, Joelle Botti1393, German Bou1423, Marina Bouche1053, Marion Bouchecareilh1348, Marie-Josee Boucher1925, Michael E Boulton486, Sebastien G Bouret1951, Patricia Boya134, Michael€ Boyer-Guittaut1362, 40 Peter Bozhkov1167, Nathan Brady377, Vania MM Braga474, Claudio Brancolini2026, Gerhard H Braus356, Jose M Bravo-San Pedro1308, Lisa A Brennan325, Emery H Bresnick2051, Patrick Brest496, Dave Bridges1966, Marie-Agnes Bringer125,491,1473, Marisa Brini1844, Glauber C Brito1328, Bertha Brodin639, Paul S Brookes1898, Eric J Brown355, Karen Brown1711, Hal E Broxmeyer485, Alain Bruhat492,1281,1356, Patricia Chakur Brum1917, John H Brumell450, Nicola Brunetti-Pierri318,1186, Robert J Bryson-Richardson792, Shilpa Buch1799, Alastair M Buchan1841, Hikmet Budak1036, 45 Dmitry V Bulavin119,512,1811, Scott J Bultman1814, Geert Bultynck674, Vladimir Bumbasirevic1489, Yan Burelle1374, Robert E Burke217,218, Margit Burmeister1772, Peter Butikofer€ 1492, Laura Caberlotto2016, Ken Cadwell907, Monika Cahova113, Dongsheng Cai25, Jingjing Cai2132, Qian Cai1032, Sara Calatayud2036, Nadine Camougrand1360, Michelangelo Campanella1721, Grant R Campbell1544, Matthew Campbell1265, Silvia Campello564, Robin Candau1791, Isabella Caniggia2012, Lavinia Cantoni568, Lizhi Cao117, Allan B Caplan1677, Michele Caraglia1066, Claudio Cardinali1058, 50 Sandra Morais Cardoso1598, Jennifer S Carew209, Laura A Carleton885, Cathleen R Carlin102, Silvia Carloni2031, CONTACT Daniel J Klionsky [email protected] Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109-2216. © 2016 Taylor & Francis Group, LLC 2 D. J. KLIONSKY ET. AL. Sven R Carlsson1284, Didac Carmona-Gutierrez1664, Leticia AM Carneiro315, Oliana Carnevali985, Serena Carra1335, Alice Carrier237, Bernadette Carroll911, Caty Casas1341, Josefina Casas1131, Giuliana Cassinelli327, Perrine Castets1481, Susana Castro-Obregon215, Gabriella Cavallini1864, Isabella Ceccherini576, Francesco Cecconi256,563,1908, Arthur I Cederbaum463, ValentınCena~ 200,1298, Simone Cenci2095,1340, Claudia Cerella448, Davide Cervia2025, 55 Silvia Cetrullo1497, Hassan Chaachouay2059, Han-Jung Chae188, Andrei S Chagin642, Chee-Yin Chai634,636, Gopal Chakrabarti1521, Georgios Chamilos1621, Edmond YW Chan1157, Matthew TV Chan182, Dhyan Chandra1017, Pallavi Chandra556, Chih-Peng Chang829, Raymond Chuen-Chung Chang1674, Ta Yuan Chang348, John C Chatham1452, Saurabh Chatterjee1934, Santosh Chauhan535, Yongsheng Che63, Michael E Cheetham1279, Rajkumar Cheluvappa1805, Chun-Jung Chen1168, Gang Chen606,1697, Guang-Chao Chen10, Guoqiang Chen1093, Hongzhuan Chen1092, 60 Jeff W Chen1533, Jian-Kang Chen373, Min Chen251, Mingzhou Chen2137, Peiwen Chen1845, Qi Chen1695, Quan Chen173, Shang-Der Chen139, Si Chen328, Steve S-L Chen11, Wei Chen2158, Wei-Jung Chen840, Wen Qiang Chen993, Wenli Chen1128, Xiangmei Chen1148, Yau-Hung Chen1172, Ye-Guang Chen1266, Yin Chen1465, Yingyu Chen967,969, Yongshun Chen2168, Yu-Jen Chen723, Yue-Qin Chen1160, Yujie Chen1224, Zhen Chen342, Zhong Chen2156, Alan Cheng1723, Christopher HK Cheng185, Hua Cheng1749, Heesun Cheong825, Sara Cherry1859, Jason Chesney1724, 65 Chun Hei Antonio Cheung828, Eric Chevet1377, Hsiang Cheng Chi141, Sung-Gil Chi665, Fulvio Chiacchiera311, Hui-Ling Chiang972, Roberto Chiarelli1848, Mario Chiariello236,575,585, Marcello Chieppa846, Lih-Shen Chin722, Mario Chiong1302, Gigi NC Chiu889, Dong-Hyung Cho685, Ssang-Goo Cho659, William C Cho996, Yong-Yeon Cho106, Young-Seok Cho1079, Augustine MK Choi2128, Eui-Ju Choi665, Eun-Kyoung Choi390,403,694, Jayoung Choi1582, Mary E Choi2126, Seung-Il Choi2149, Tsui-Fen Chou415, Salem Chouaib398, Divaker Choubey1593, Vinay Choubey1963, 70 Kuan-Chih Chow833, Kamal Chowdhury741, Charleen T Chu1879, Tsung-Hsien Chuang838, Taehoon Chun666, Hyewon Chung661, Taijoon Chung992, Yuen-Li Chung1209, Yong-Joon Chwae19, Valentina Cianfanelli257, Roberto Ciarcia1797, Iwona A Ciechomska897, Maria Rosa Ciriolo1900, Mara Cirone1057,Sofie Claerhout1715, Michael J Clague1719, Joan Claria1476, Peter GH Clarke1708, Robert Clarke364, Emilio Clementi1060,1416,Cedric Cleyrat1803, Miriam Cnop1384, Eliana M Coccia582, Tiziana Cocco1478, Patrice Codogno1393,Jorn€ Coers274, Ezra EW Cohen1552, 75 David Colecchia236,575,585, Luisa Coletto8,Nuria S Coll124, Emma Colucci-Guyon523, Sergio Comincini1852, Maria Condello586, Katherine L Cook2105, Graham H Coombs1955, Cynthia D Cooper2108, J Mark Cooper1413, Isabelle Coppens609, Maria Tiziana Corasaniti1405, Marco Corazzari490,1908, Ramon Corbalan1585, Elisabeth Corcelle-Termeau253, Mario D Cordero1923, Cristina Corral-Ramos1306, Olga Corti514,1124, Andrea Cossarizza1789, Paola Costelli2022,Safia Costes1537, Susan L Cotman732, Ana Coto-Montes960, Sandra Cottet574,1709, Eduardo Couve1318, 80 Lori R Covey1029, L Ashley Cowart773, Jeffery S Cox1555, Fraser P Coxon1445, Carolyn B Coyne1869, Mark S Cragg1943, Rolf J Craven1700, Tiziana Crepaldi2024, Jose L Crespo1317, Alfredo Criollo1302, Valeria Crippa566, Maria Teresa Cruz1595, Ana Maria Cuervo27, Jose M Cuezva1294, Taixing Cui1931, Pedro R Cutillas1001, Mark J Czaja28, Maria F Czyzyk-Krzeska1591, Ruben K Dagda, Uta Dahmen1422, Chunsun Dai811, Wenjie Dai1202, Yun Dai2090, Kevin N Dalby1967, Luisa Dalla Valle1844, Guillaume Dalmasso1357, Marcello D’Amelio565, Markus Damme189, Arlette Darfeuille-Michaud1357, 85 Catherine Dargemont964, Victor M Darley-Usmar1451, Srinivasan Dasarathy206, Biplab Dasgupta203, Srikanta Dash1270, Crispin R Dass244, Hazel Marie Davey9, Lester M Davids1579, David Davila228, Roger J Davis1752, Ted M Dawson612, Valina L Dawson614, Paula Daza1922,