WORLD PRECISION INSTRUMENTS Instrumenting scientific ideas Microinjection & Transfection 2016-2017 Come see us at The Allied Genetics 2016 Conference • July 13-17 • Booths # 623 & 816/818 Everything you need for Microinjection Microinjection processes use either metal microinjection Recently, WPI introduced its customizable Microinjection needles or glass micropipettes to inject small liquid System with everything you need to get started. We can volumes. For example, genetic material may be inserted help you customize your system with many options and into a living cell, a drug introduced into an eye or brain, microinjection system accessories. The basic system or fluid injected into a muscle. Typically, microinjection includes: is performed under a microscope. A stereotaxic frame l Microinjection pump like the PV820 Pneumatic setup may be required. PicoPump l LED lighted microscope base with a PZMIII Stereo Microscope and an articulating mirror l Micromanipulator l Many accessories WPI offers a variety of pumps along with special syringes, stereotaxic frames, glass capillaries and needles. The setup you choose depends on the size of your microinjection aliquots, the volume to be injected and the size of needle or glass tip you choose. In addition, we offer an electroporator for transfection procedures. Whether you are working with Danio rerio (zebrafish), Xenopus, Drosophila or Caenorhabditis elegans, we can help you customize a microinjection system for your application. Options for Customizing Your System INJECTOR ✱ PV820 Pneumatic PicoPump with Hold Pressure • PV830 Pneumatic PicoPump with Hold Designed to simplify intracellular injection and a variety Microprocessor- The versatile Pressure and Vacuum of other micro in jec tion tasks, WPI's PicoPumps use UMP3 controlled injector uses • Nanoliter2010 carefully regulated air pres sures for se cur ing cells and injecting them with fluid. Injected volumes range from Nanoliter 2010 microsyringes to UltraMicroPump • UMPIII picoliters to nanoliters. uses direct pis ton deliver picoliter dis place ment. volumes. MICROSCOPE ✱ PZMIII-MI Microscope PZMIII-MI LED Lighted with lighted base and Microscope Base with articulating articulating mirror mirror and variable light intensity. Dual reflection lens/mirror system PZMIII Precision Stereo PZMIII Precision • provides transmitted brightfield/ Stereo Zoom Zoom Microscope psuedo-darkfield illumintation. Microscope, available in binocular and trinocular (shown). For more information, see www.wpiinc.com/microinjection PULLERS PUL-1000 is a microprocessor controlled horizontal puller for making glass micropipettes or microelectrodes used in ✱ PUL1000 Microprocessor- intracellular recording, microperfusion or microinjection. controlled 4-Step It offers programmable sequences of up to four steps micropipette puller with heating, force, movement and cooling time. This • PMP-102 Programmable allows graduated cycles for applications like patch clamp Multipipette Puller recording. MANIPULATOR ✱ M3301 Manual Weighing just 550 grams, Micromanipulator the M3301 is a well- • KITE Manual built micro manip u la tor M4C Microscope Micromanipulatorr that outsells all others Stage Adapter • DC3001 Motorized world wide for high preci- Micromanipulator sion experiments where • SN-PCZ-50 Miniature Piezo mag ni fi ca tion is in the range Micromanipulator with of up to 250×. controller • M4C Microscope Stage Adapter LIGHTS ✱ Z-LITE-186 Fiber optic illuminator with (500186) Bifurcated Light Guides • ROF-UV LED ringlight for UV • LED-Lite Modular LED Light Source with Exchangeable Z-LITE-186 LEDS • 504134 LED Ring light The 504134 Ring Light Adapter has 72 LEDs. Max opening: 61mm. ACCESSORIES • Nanofil Microliter syringes Use the E2XX jars MicroFil is a flexible and • MicroFil for backfilling to store up to 30 reusable glass needle as small glass needles micropipettes, filled as 36 gauge for backfilling • Glass capillaries or unfilled, up to micropipettes. • Pipetters three inches in length. • MicroTip pre-pulled pipettes • E2XX Micropipette Storage Jar • 801566/801963 Vacuum Z-MOLDS Microinjection and Pump for use with the PV830 Transplantation Molds (4 per kit) are • Fluorodish Optical glass designed for zebrafish research. The molds bottom dishes are turned up-side down and placed in liquid agarose gel and are easily removed • M10 or M-3 Manipulator base once it has solidified. Pipette the embryos • Z-MOLDS Microinjection and into the grooves. The embryos self-align. Translplantation Molds • Many surgical instruments CUY21-EDIT-II Electroporator is a combination square wave and ELECTROPORATOR exponential wave form generator with single and dual pulse modes. It • CUY21-EDIT-11 has single and dual pulse modes for square and decaying pulses. You Electroporator can set pulses emitting constant current (mA) as well as voltage (V) and generate multiple waveforms. It offers built-in resistance measurement and a measured output recorder. Pneumatic PicoPumps Repeatable microinjection in volumes ranging from picoliters to nanoliters Designed to simplify intracellular injection and a variety of other pressure, and suction are adjusted indepen dent ly by control knobs and micro in jec tion tasks, WPI's PicoPumps use carefully regulated air pres- in di ca tor gauges on the front panel. Injection pressure is controlled by a sures for se cur ing cells and injecting them with fluid. Injected volumes 20-turn regulator on the front panel. A built-in timing circuit allows precise range from picoliters to nanoliters. Separate ports supply positive and control of the amount of time that the injection pressure is applied to the negative pressure—pos i tive pressure for high-pressure ejection, and output port. Time intervals can range from 10 seconds down to 10 ms suction for supporting the cell or for filling the pipette from the tip. A or less, depending on the eject pressure setting. The injection pressure second pressure port maintains a low positive “holding” pressure to interval can be triggered manually on the front panel, by footswitch, or by the injecting pipette between injection pulses, to prevent fluid uptake computer controlled TTL pulse. A 5-volt moni tor output provides a logic- through capillary action or diffusion. Timing, ejection pressure, holding level pulse for your computer or other moni tor ing device. The most recognized picopump in the world! For a complete list of pre-pulled micropipettes, see µTips™, or call us with your special requirements. PV830 — Eject pressure, Hold pressure, and Vacuum are all available, Eject and Hold pressure by a precision timing circuit that controls a controlled by separate reg u la tors on the front panel. Eject pressure solenoid valve. Vacuum is used to fill pipettes from the tip or to secure supplies a high-pressure pulse for injecting fluid. Hold pressure, which a floating cell during microinjection. Vacuum is regulated the same way, is not sufficient to cause fluid ejection, is used to prevent back filling of by a 20-turn knob on the front panel. Vacuum may be switched from the pipette by capillary action or diffusion when the solenoid is inactive. regulated vacuum to atmo sphere by using the pneumat ic toggle on the Pressure in the injection pipette is auto mat i cal ly switched between front panel. Vacuum can also be routed to the eject port. Each PicoPump is supplied with a 5430-ALL kit that includes two PicoNozzles and tubing to connect the holders to the pressure and vacuum ports. SYS-PV820 PicoPump w/ hold pressure SYS-PV830 PicoPump w/ hold pressure and vacuum Specify line voltage All PicoPumps require external vacuum source — see below. OPTIONAL ACCESSORIES 3260 Foot Switch 1 2932 Rack Mount Kit, 3 ⁄ 2-in. high (PV820) 1 2933 Rack Mount Kit, 5 ⁄4-in. high (PV830) 5430-10 PicoNozzle Kit (MPH6S for 1.0 mm pipette & 5-ft tubing assembly) 5430-12 PicoNozzle Kit (MPH6S for 1.2 mm pipette & 5-ft tubing assembly) New PicoNozzle Kit 5430-ALL (included) 5430-15 PicoNozzle Kit (MPH6S for 1.5 mm pipette & 5-ft tubing assembly) allows micropipettes to be securely 5430-20 PicoNozzle Kit (MPH6S for 2.0 mm pipette & 5-ft tubing assembly) mount ed in micropositioners for stable 5430-ALL PicoNozzle Kit (for 1.0, 1.2, 1.5, and 1.65 mm pipettes & 5-ft tubing assembly) 75122-110 Replacement Gaskets for PicoNozzle — 1.0 mm, green, package of 10 axial air delivery. Because air enters the 75122-210 Replacement Gaskets for PicoNozzle — 1.2 mm, black, package of 10 pipette axially, lateral whipping during 75122-310 Replacement Gaskets for PicoNozzle — 1.5 mm, blue, package of 10 injection is eliminated. 75122-410 Replacement Gaskets for PicoNozzle — 1.65 mm, red, package of 10 MPH6S Micropipette Holder (specify 1.0, 1.2, 1.5 or 2.0 mm) MPH6R Micropipette Holder (specify 1.0, 1.2, 1.5 or 2.0 mm) 3316 Replacement Input Kit ORDER TOLL-FREE: 866-606-1974 (U.S. only) 2 World Precision Instruments • Tel: 941-371-1003 • Fax: 941-377-5428 • E-mail: [email protected] • Internet: www.wpiinc.com PV820 offers separate regulated Hold and Ejection pressure, used timing has been set. Although reg u lat ed vac u um is not provid ed in this mod- to maintain a low pressure in the pipette between injections to prevent el, suction can be provid ed by con nect ing a vacu um source to the vacu um unwanted fluid uptake by capillary action or dif­fu sion. A pre ci sion timing port on the rear panel. Suction is then available through the pressure ports. circuit switches from Eject pressure to Hold pressure auto mat i cal ly, once PICOPUMP SPECIFICATIONS PV820 PV830 PRESSURE PRESSURE INPUT 0 to 150 psi 0 to 150 psi PRESSURE OUTPUT 0.3 to 90 psi * 0.3 to 90 psi PULSE WIDTH (10-turn dial) 10 ms to 10 s in Timed Mode 10 ms to 10 s in Timed Mode REGULATOR ACCURACY 0.1% (20-turn dial) * 0.1% (20-turn dial) * REGULATOR REPEATABILITY 0.05 psi * 0.05 psi * GAUGE ACCURACY 3% at full scale * 3% at full scale * 1 1 INPUT CONNECTOR Quick Connect ( ⁄4 in.