Immunology and Inflammation Inflammation is a critical, signal-mediated response to potential signs of danger and damage. While acute inflammation is important to the immune response, chronic, inappropriate inflammation can cause tissue destruction and lead to disease (e.g., allergies, autoimmune disorders). Cayman offers a wide range of tools to help you study the factors related to innate and adaptive immune responses. · Assays and mass spec standards for the quantification of bioactive lipids · Corticosteroid receptor agonists, mast cell stabilizers, and antihistamines · Tools to study citrullination and carbamylation · TLR and chemokine receptor modulators and NETosis assays LOOK INSIDE TO LEARN MORE · Immunopeptidome profiling services Page 1 mast cell allergen eosinophil target protein Inflammatory IgE Lipid Mediators PAD Page 2 cytokines citrullinated CysLTs cytokines protein Allergy & Asthma PGs CysLTs histamines ACPA Page 3 histones Autoimmunity neutrophil LL-37 NE MPO Page 4 · Immunotherapeutics LTB 4 · Adaptive Immunity receptor TLR T cell PAMPs Page 5 SPMs T cell Innate Immunity receptor MHC Page 7 COOH · Infectious Disease macrophage · Immunosuppressants DHA (DPA or EPA or AA) APC The cellular environment during infection (or allergen exposure), immune activation, and subsequent inflammation involves a highly complex and well-coordinated team of mediators. This includes mast cells that react to danger by releasing histamines, pro-inflammatory cytokines, and eicosanoids and neutrophils/eosinophils that respond to these signals with an antimicrobial arsenal that involves NETs. Certain protein modifications, like citrullination, can alter self-epitopes and elicit an autoantibody response via T cells. Professional APCs, such as macrophages, activate T cells by displaying a MHC. SPMs, byproducts of DHA and other fatty acids, are generated later to initiate the resolution of inflammation. 2019 CAYMAN CHEMICAL · 1180 EAST ELLSWORTH ROAD · ANN ARBOR, MI 48108 · USA · (800) 364-9897 WWW.CAYMANCHEM.COM Inflammatory Lipid Mediators Cayman offers a wide range of tools to study biochemical mediators released during the inflammatory process. Our core business is built around the production of bioactive lipids, including the manufacture of high-purity lipid standards and assays for their measurement, as well as biochemicals to disrupt key eicosanoid mediator production or signaling. We lead the industry as a resource for prostaglandins, leukotrienes, and specialized pro-resolving mediators pertinent to the study of immune activation and inflammation. Leukotrienes Specialized Pro-Resolving Mediators Item No. Product Name Item No. Product Name 20110 Leukotriene B4 90410 Lipoxin A4 520111 Leukotriene B4 ELISA Kit* 590410 Lipoxin A4 ELISA Kit 120114 BLT 1 Receptor Polyclonal Antibody 90415 15(R)-Lipoxin A4 20210 Leukotriene C4 90420 Lipoxin B4 20310 Leukotriene D4 10878 Maresin 1 20410 Leukotriene E4 501150 Maresin 1 ELISA Kit 501060 Leukotriene E4 ELISA Kit 16369 Maresin 2 500390 Cysteinyl Leukotriene ELISA Kit* 17007 MCTR1 20001 Cysteinyl Leukotriene HPLC Mixture I 17008 MCTR2 20002 Cysteinyl Leukotriene HPLC Mixture II 19067 MCTR3 120500 CysLT 1 Receptor Polyclonal Antibody 19064 PCTR1 14713 CysLT 1 Receptor Polyclonal FITC Antibody 10010390 Protectin D1 10012554 Resolvin D1 Prostaglandins 13060 17(R)-Resolvin D1 Item No. Product Name 500380 Resolvin D1 ELISA Kit 501001 tetranor-PGDM ELISA Kit 10007279 Resolvin D2 12010 Prostaglandin D2 501120 Resolvin D2 ELISA Kit 512031 Prostaglandin D2 ELISA Kit* 13834 Resolvin D3 512011 Prostaglandin D2-MOX ELISA Kit* 13835 Resolvin D4 14010 Prostaglandin E2 10007280 Resolvin D5 314010 Prostaglandin E2-d4 18702 SPM D-series LC-MS Mixture 514010 Prostaglandin E2 ELISA Kit - Monoclonal* 10007848 Resolvin E1 516521 11β-Prostaglandin F2α ELISA Kit Common SPMs listed; methyl ester forms, internal standards, and LC-MS mixtures *Also available: express kits also available COOH COOH COOH COOH AA (90010) DPA (90165) DHA (90310) EPA (90110) www.caymanchem.com 45 -2 Request the full Specialized 15 5 2 15 COOH OOH COOH COOH COOH COOH OOH 18(R)-HpEPE *15(S)-HpETE (44720) *5(S)-HpETE (44230) OOH 5 HOO 13(R)-HpDPA OH 5 COOH OOH HOO 17(S)-HpDHA (13185) COOH OH Pro-Resolving Mediators wall poster at COOH 10(S),17(S)-DiHDHA (PDX) (10008128) COOH OOH *5(S),15(S)-DiHpETE COOH O COOH 18(R)-HEPE HO Resolvin E3 OH HO 13(R)-HDPA OH 16(S),17(S)-epoxy Protectin 5 O COOH O OH OH OH OH OOH NH OH COOH COOH COOH OH COOH www.caymanchem.com/Literature OH COOH HO H2N O S *5(S),6(S)-epoxy-15(S)-DiHETE COOH NH COOH OH Resolvin T4 (18440) COOH 5(S)-hydroperoxy- Resolvin T1 (18437) Resolvin E2 (13827) OH Protectin D1/Neuroprotectin D1 (10010390) OH PCTR1 (19064) 18(R)-HEPE OH HO OH OH OH OH COOH COOH COOH OH COOH OH H N H N 2 2 O OH OH COOH OH HO OH O S HOOC S HO OH OH COOH OH NH COOH COOH Lipoxin A4 (90410) Lipoxin B4 (90420) OH (15(R)-Lipoxin A4, 90415) COOH Resolvin E1 (10007848) Resolvin T2 (18438) Resolvin T3 (18439) PCTR2 (19065) Protectin Family PCTR3 (19066) OH 5(S),6(S)-epoxy- 18(R)-HEPE *Have not been shown to have pro-resolving properties in published literature E-Reso COOH HOO COOH 5 15 12 15 HOO 1 CAYMAN CHEMICAL · 1180 EAST ELLSWORTH ROAD · ANN ARBOR, MI 48108HO · USA · (800) 364-9897 17(S)-HpDHA (13185) WWW.CAYMANCHEM.COM 4(S)-hydroperoxy- 17(S)-HDHA 5 COOH O O HOO OH OOH O COOH COOH COOH COOH COOH HO 13(S),14(S)-epoxy-Maresin 14(S)-HpDHA 14(S),20(R)-DiHDHA (19139) HO 4(S),5(S)-epoxy- 7(S),8(S)-epoxy- 7(S)-hydroperoxy- 17(S)-HDHA HO 17(S)-HDHA HO 17(S)-HDHA O HOOC NH NH H HOOC 2 N S HO COOH O HOOC OH OH HO COOH HO OH OH HO COOH OH COOH COOH HO COOH OH MCTR1 (17007) OH HO HO HO OH HO OH Resolvin D3 (13834) Resolvin D4 (13835) Resolvin D2 (10007279) Resolvin D1 (10012554) Resolvin D5 (10007280) COOH NH COOH (17(R)-Resolvin D1, 13060) 2 Resolvin D6 (10484) H Hydrolase HOOC N S 14(S)-HDHA (15253) O OH OH OH COOH SPECIALIED PRO-RESOLING MEDIATORS Maresin 1 (10878) MCTR2 (17008) Soluble Epoxide The resolution of inflammation is an active process that is driven by unique signaling molecules. An important group of these specialized pro-resolving Hydrolase NH HO 2 mediators (SPMs) is derived from polyunsaturated fatty acids (arachidonic acid (AA), eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA), and S docosahexaenoic acid (DHA)) that are liberated during the inflammatory process. Studies have demonstrated that these SPMs, which include resolvins, HOOC OH lipoxins, maresins, and protectins, evoke cellular and tissue responses that help conclude an inflammatory episode. Moreover, the addition of nanomolar OH COOH COOH concentrations of SPMs to an inflammatory milieu can have profound effects, such as reducing leukocyte infiltration, enhancing phagocytosis, and OH OH Maresin 2 (16369) MCTR3 (19067) reducing pain. Cayman Chemical, a leader in the synthesis of lipid mediators, offers an array of SPMs to help make your research possible. COOH 14(S),21(R)-HDHA Allergy & Asthma Immune activation and inflammation are both characteristics of an allergic or asthmatic response. Cayman’s line of allergy and asthma research products includes tools for measuring and disrupting eicosanoid mediators and assays to assess ovalbumin-induced immune responses. Key corticosteroid receptor agonists, mast cell stabilizers, histamine receptor antagonists/inverse agonists, phosphodiesterase inhibitors, and β-adrenergic receptor agonists are also available. DiHOMEs: Potential drivers of allergic responses and thermogenic activity Dihydroxy metabolites of linoleic acid may be biomarkers (±)9(10)-DiHOME ELISA Kit for the development of asthma and important mediators Item No. 501710 of fatty acid metabolism. Cayman offers competitive ELISAs to quantify 9(10)-DiHOME and 12(13)-DiHOME (±)12(13)-DiHOME ELISA Kit in plasma and serum. Item No. 501720 Corresponding unlabeled and deuterated standards for EpOMEs and DiHOMEs are also available for quantification by mass spec Ovalbumin Assay Kits β-Adrenergic Receptor Agonists Item No. Product Name Item No. Product Name 500840 Anti-Ovalbumin IgE (mouse) ELISA Kit 14985 Clenbuterol (hydrochloride) 500830 Anti-Ovalbumin IgG1 (mouse) ELISA Kit 15584 Formoterol (hemifumarate) 16009 Salmeterol (xinafoate) Corticosteroid Receptor Agonists Over 10 β-adrenergic receptor agonists available online Item No. Product Name Antihistamines 15407 Budesonide Item No. Product Name 20739 Hydrocortisone 16967 Astemizole 18026 Triamcinolone acetonide 14637 Clemastine (fumarate) Over 10 corticosteroid receptor agonists available online 15625 Loratadine Mast Cell Stabilizers 14615 Meclizine (hydrochloride) Item No. Product Name Over 30 histamine receptor antagonists and/or inverse agonists available online 22173 Compound 48/80 (hydrochloride) 21379 Cromolyn (sodium salt hydrate) Conveniently Detect Histamine 23994 Lodoxamide Histamine EIA Kit - Item No. 589651 Phosphodiesterase Inhibitors · Measure histamine in plasma, serum, Item No. Product Name bronchoalveolar lavage, tissue extracts, and 14832 Ibudilast cell culture supernatant samples 15141 Roflumilast · Assay 33 samples in duplicate 10011132 Rolipram · Incubation: Overnight 10008671 Sildenafil Manufactured by Bertin Bioreagent Over 10 PDE inhibitors as well as related adenosine receptor antagonists available online WWW.CAYMANCHEM.COM CAYMAN CHEMICAL · 1180 EAST ELLSWORTH ROAD · ANN ARBOR, MI 48108 · USA · (800) 364-9897 2 Autoimmunity Autoimmune disorders, such as rheumatoid arthritis and systemic lupus erythematosus, are tied to post-translational modifications