life Editorial The Genetic Code: Francis Crick’s Legacy and Beyond 1,2 Koji Tamura 1 DepartmentEditorial of Biological Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika-ku,The Genetic Tokyo 125-8585, Code: Japan; Francis [email protected]; Crick’s Tel.: +81-3-5876-1472 Legacy and Beyond 2 Research Institute for Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, ChibaKoji 278-8510, Tamura 1,2 Japan Academic1 Department Editor: David of Biological Deamer Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Received:Katsushika 22 August-ku, 2016; Tokyo Accepted: 125-8585, 23Japan August; [email protected] 2016; Published:; Tel: +81- 253-5876 August-1472 2016 2 Research Institute for Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan Academic Editor: David Deamer FrancisReceived: Crick 22 August (Figure 20161); wasAccepted: born 23 onAugust 8 June 2016 1916,; Published: in Northampton, date England, and passed away on 28 July 2004, in La Jolla, California, USA. This year, 2016, marks the 100th anniversary of his birth. A drastic changeFrancis in Crick the life(Figure sciences 1) was was born brought on 8 June, about 1916, byin Northampton, the discovery England of the, double and passed helical away structure on 28 July, 2004, in La Jolla, California, USA. This year, 2016, marks the 100th anniversary of his of DNAbirth. by JamesA drastic Watson change andin the Francis life science Cricks was in brought 1953 [about1], eventually by the discovery leading of the to double the deciphering helical of the geneticstructure code of [ 2DNA]. The by elucidation James Watson of theand geneticFrancis codeCrick wasin 1953 one [1], of theeventually greatest leading discoveries to the of the 20th century.deciphering The geneticof the genetic code code is an [2]. algorithm The elucidation that connects of the genetic 64 RNA code triplets was one to of 20 the amino greate acids,st and functionsdiscoveries as the Rosetta of the 20th stone century of molecular. The genetic biology. code is an algorithm that connects 64 RNA triplets to 20 amino acids, and functions as the Rosetta stone of molecular biology. FigureFigure 1. Sir 1.Francis Sir Francis Crick, Crick, La La Jolla Jolla 1982, 1982, Photograph Photograph by Norman Norman Seeff Seeff.. Credit: Credit: Norman Norman Seeff Seeff Productions. Productions. At the age of 60, Crick moved to La Jolla from Cambridge, England, and shifted his focus to the Atbrain the age and ofhuman 60, Crick consciousness. moved toHe La tackled Jolla this from subject Cambridge, for the last England, 28 years of and his shiftedlife. His hislife- focuslong to the brain andinterest human was consciousness.the distinction between He tackled the living this and subject the non for-living the last, which 28 years motivated of his his life. research His life-long interestcareer was the. Crick distinction was arguably between one theof the living 20th andcentury’s the non-living, most influential which scientists motivated, and hishe researchdevoted career. Crick washims arguablyelf to science one until of thehis death. 20th century’s most influential scientists, and he devoted himself to Francis Crick continued to exercise his intellectual abilities throughout his life. His research science until his death. style was characterized by collaborations with outstanding partners, James Watson in discovering Francis Crick continued to exercise his intellectual abilities throughout his life. His research Life 2016, 6, 36; doi:10.3390/life6030036 www.mdpi.com/journal/life style was characterized by collaborations with outstanding partners, James Watson in discovering the Life 2016, 6, 36; doi:10.3390/life6030036 www.mdpi.com/journal/life Life 2016, 6, 36 2 of 5 Life 2016, 6, 36 2 of 5 structure of DNA, Sydney Brenner in cracking the genetic code, Leslie Orgel in probing the origins of life,the structure and Christof of DNA Koch, in Sydney understanding Brenner humanin cracking consciousness. the genetic Francis code, Crick Leslie was Or nevergel in modest probing in the his choiceorigins of of scientific life, and problems Christof [Koch3] and in was understanding like “the conductor human ofconsciousness. the scientific orchestra”Francis Crick [4]. Hewas always never discussedmodest in his ideas,choice whichof scientific helped problems in the progress [3] and hewas made like “the in science. conductor Interestingly, of the scientific his son, orchestra” Michael, then[4]. He 12 always years old, discussed was the his first ideas, person which to helped read the in earliest the progress written he description made in science. of the Interestingly, genetic code. Crickhis son wrote, Michael the following, then 12 years in a letterold, was to Michael, the first person to read the earliest written description of the genetic code. Crick wrote the following in a letter to Michael, “ ... Now we believe that the D.N.A. is a code. That is, the order of the bases (the letters) makes “one…Now gene we different believe fromthat anotherthe D.N.A. gene is (just a code. as oneThat page is, the of printorder is of different the bases from (the another).letters) makes You can one genenow different see how Naturefrom another makes gene copies (just of theas one genes. page Because of print if is the different two chains from unwindanother). into You two can separate now see howchains, Nature and ifmakes each chaincopies thenof the makes genes another. Because chain if the come two together chains onunwind it, then into because two separat A alwayse chains, goes andwith if T,each and chain G with then C, makes we shall another get two chain copies come where together. ” on (Figure it, then2). because A always goes with T, and G with C, we shall get two copies where…” (Figure 2). Figure 2. LetterLetter from from Francis Francis Crick Crick to his to son his, son,Michael Michael,, explaining explaining his and his Watson and Watson’s’s discovery discovery of the ofstructure the structure of DNA. of DNA.The letter The is letter the earliest is the earliest written written description description of the ofgenetic the genetic mechanism mechanism on 19 onMarch, 19 March 1953. 1953.Credit: Credit: Wellcome Wellcome Library, Library, London. London. This is the fundamental principle of biology. The big questions that arose after the discovery of the structure of DNA were “how is the code used?” and “what is it a code for?” Francis Crick turned his attention to find answers to these questions for the next 13 years. George Gamow, who is famous for the Big Bang theory, founded the 20-member “RNA Tie Club” with Watson, to discuss Life 2016, 6, 36 3 of 5 This is the fundamental principle of biology. The big questions that arose after the discovery of the structure of DNA were “how is the code used?” and “what is it a code for?” Francis Crick turned his attention to find answers to these questions for the next 13 years. George Gamow, who is Life 2016, 6, 36 3 of 5 famous for the Big Bang theory, founded the 20-member “RNA Tie Club” with Watson, to discuss the transmission of information by DNA. RNA-illustrated neckties were provided to all members, and a the transmission of information by DNA. RNA-illustrated neckties were provided to all members, golden tiepin with the abbreviation for one of the 20 amino acids was given to each member. Crick and a golden tiepin with the abbreviation for one of the 20 amino acids was given to each member. was “TYR” (tyrosine). Crick’s famous “adaptor hypothesis” was prepared for circulation in the RNA Crick was “TYR” (tyrosine). Crick’s famous “adaptor hypothesis” was prepared for circulation in Tie Club [5], but when Paul Zamecnik and collaborators discovered transfer RNA (tRNA) [6], Crick the RNA Tie Club [5], but when Paul Zamecnik and collaborators discovered transfer RNA (tRNA) did not believe that it was indeed the adaptor, because of its unexpectedly large size. Crick insisted [6], Crick did not believe that it was indeed the adaptor, because of its unexpectedly large size. Crick that there would be 20 different adaptors for the amino acids, and that they would bring the amino insisted that there would be 20 different adaptors for the amino acids, and that they would bring acids to join the sequence of a nascent protein. A manuscript entitled “Ideas on protein synthesis the amino acids to join the sequence of a nascent protein. A manuscript entitled “Ideas on protein (October, 1956)” remains extant (Figure3). Crick spoke about “The Central Dogma” at a Society for synthesis (October, 1956)” remains extant (Figure 3). Crick spoke about “The Central Dogma” at a Experimental Biology symposium on “The Biological Replication of Macromolecules”, held at the Society for Experimental Biology symposium on “The Biological Replication of Macromolecules”, University College London in September, 1957. The Central Dogma holds true even today, and is held at the University College London in September, 1957. The Central Dogma holds true even another example of Crick’s genius. today, and is another example of Crick’s genius. Figure 3. The earliest written description of “The Central Dogma” in a manuscriptmanuscript entitledentitled “Ideas on protein synthesis (October(October,1956)”. 1956)”. Credit:Credit: WellcomeWellcome Library,Library, London. London. In 1961, Francis Crick, Sydney Brenner, Leslie Barnett, and Richard Watts-Tobin first demonstrated the three bases of DNA code for one amino acid [7]. That was the moment that scientists cracked the code of life. However, ironically, the first decoding of the “word” of the genetic code was reported in the same year by a non-member of the RNA Tie Club, Marshall Nirenberg, who spoke at the International Biochemical Congress in Moscow.