AgroLifeAgroLife Scientific Scientific JournalJournal - -Volume Volume 8, 8, Number Number 1, 1,2019 2019 effects of degradation, improving soil Kachinsky N.A., 1965. Soil physics. Moscow. Science, ISSNISSN 2285-5718; 2285-5718; ISSN ISSN CD-ROM CD-ROM 2285-5726;2285-5726; ISSN ONLINEONLINE 2286-0126; 2286-0126; ISSN-L ISSN-L 2285-5718 2285-5718 valuation, conducting monitoring studies, and Higher School. 265 p. Krivenkov N.P., 1967. The effect of soil density on the so on (French et al., 1979; Tansley, 1935). flow of nutrients into plants. Abstract of dissertation Pichia (Kodamaea) ohmeri CMGB-ST19 - A NEW STRAIN The stability of the structural organization of Ph.D. Agricultural Institute. Leningrad. 18 p. WITH COMPLEX BIOTECHNOLOGICAL PROPERTIES soils and, due to it, water, heat, gas and food Lee K.T., Foster R.C., 1991. Soil fauna and soil structure. Austral Y. Soil Res. Vol. 29, No. 6, 745- regimes are determined in the general result of Viorica CORBU, Tatiana VASSU, Ortansa CSUTAK their physico-mechanical properties. This 775. French N., Ryshkovsky L., Titlyanova A.A. et al., 1979. circumstance reflects the importance of Comparison of the structure of organic matter in University of Bucharest, Faculty of Biology, 1-3 Aleea Portocalelor, studying the physicomechanical properties of different ecosystems. Tom. 10, No. 2, 8-21. 060101 Bucharest, Romania soils, which are the basis for predicting the Friedland V.M., 1972. The structure of the soil cover. effects of various kinds of anthropogenic stress Moscow. Think. 423 p. Corresponding author email: [email protected] on the soil and soil cover. Hadas A., 1990. Directional strength in aggregates as affected by aggregate volume and by a wet/dry cycle. Such physical and mechanical characteristics of Vol. 41, No. 1, 85-93. Abstract the soil as cohesion (s), angle of internal Milanovsky E.Yu., Shein E.V., 2002. Mechanisms of friction (φ), shear resistance (τ) have a decisive formation and stability of the soil structure. Soil The yeast species Pichia ohmeri has been described as far, as biocontrol agent. The present work deals with the resistance to natural and anthropogenic impacts. complex characterization of a new yeast strain CMGB-ST19 isolated from the surface of jam roses (Rosa damascena) influence on the erosion resistance of the soil petals. The conventional taxonomy tests and the BiologMicroLog system allowed the identification of CMGB-ST19 as (Mirtskhulava, 1990). Moscow. Science, p. 83. Mirtskhulava T.E., 1990. Reliability of the functioning of Pichia ohmeri. The results were confirmed using ITS-PCR, the strain being renamed as Pichia ohmeri CMGB-ST19. agroecological systems. Bulletin of science, No. 12, Various tests were performed for determination of its biotechnological properties. P. ohmeri CMGB-ST19 presented CONCLUSIONS 80-83. good lipolytic activity and hydrolysed tributyrin releasing the butyric acid with positive impact on human health. Mirtskhulava T.E., 2001. Forecast soil washout. Reports Moreover, the strain showed antimicrobial activity against 11 Candida and Saccharomyces strains in presence of low It should be noted that with agricultural use, the of the RAAS. No. 4, 24-27. pH values. During the preliminary assays for bioethanol and biosurfactants synthesis, significant growth rates were soil is exposed to the most versatile (in terms of Oades J.M., 1991. Waters, Aggregate hierarchy in soils. recorded after 24 hours on molasses, respectively, after 72 hours on D-xylose when morphological changes were also observed related to cell stress. In conclusion, P. ohmeri CMGB-ST19 represents an interesting newly characterized Austral. J. Soil. Res. Vol. 29, No. 6, 815-828. the number of disturbing factors) and intensive yeast strain with high potential for a wide range of biotechnological applications. Reid J.B., Goss M.I., 1981. Effect of living roots of impacts, which affects the ecological situation different plant species on the aggregate stability of Key words: Pichia ohmeri, lipase, antimicrobial activity, molasses, D-xylose. of agricultural landscapes and ecosystems as a two arable Soils. Vol. 32, 521-547. whole. Rode A.A., 1947. The podzol process and soil evolution. Soil structuring under the influence of grasses M.: Geografizdat. 140 p. INTRODUCTION biotechnological potential (Al-Sweih, 2011; is most effective in areas with sufficient rainfall Snakin V.V. et al., 1995. Ecological assessment of soil Distasi et al., 2015; Kanno et al., 2017). In and on irrigated land. resistance to human impact. No. 5, 50-57. Kodamaea ohmeri belongs to the Kodamaea 2013, Kitcha et al reported good level of lipid Sudakov A.V., Petrushenko S.E., 1982. The effect of soil genus (class Ascomycetae, Saccharomycetaeae production when K. ohmeri was grown on An important task of modern agriculture is the compaction on the growth of root systems and the development of areas of ecologization of agri- accumulation of above-ground corn mass. Effect of family) which includes species isolated both crude glycerol. K. ohmeri was also reported as cultural technologies that increase the stability fertilizers and tillage on their physical properties and from environment and entomopathogenic presenting high degradation levels of patulin of soil fertility and the productivity of crop yield. Lenidrad, 10-15. organisms. K. ohmeri is well integrated in the and recommended it for patulin detoxification agroecosystems. Sheptukhov V.N., 1993. Scientific and experimental new Kodamaea genus which forms both hat- of apple derived products. A new K. ohmeri substantiation of optimization of agrophysical The results show that the annual intake of fresh shaped and spherical spores depending on the strain isolated from fish gut had high phytase properties and structure of the root zone of the soil in mating type. Subsequent, other Kodamaea activity which can be used as supplement for plant residues into the soil in winter rye crops order to increase the sustainability of crop yields. provides a significant increase in the swelling Nemchinovka. 37 p. species were described (K. anthophila, K. animal and human nutrition to liberate the of the structure compared to potatoes and the Tansley A.G., 1935. The use and abuse of vegetational kakaduensis, K. laetipori, K. nitidulidarum, K. inorganic phosphate from phytic acid from crop rotation field. concepts and terms. Ecology, No. 16, 248-307. transpacifica) proving the necessity to form a grains, increasing thus their nutritional value Vasilievskaya V.D., 1994. Soil resistance to different genus, derived from Yamadazyma (Hirimuthugoda et al., 2006; Li et al., 2007). anthropogenic impacts. // Soil-ecological monitoring which accommodate Pichia species that can Also, K. ohmeri is known as being able to REFERENCES and soil protection. Moscow, 61-79. form hat-shaped spores (Freitas et al., 2013; convert glucose to D-arabitol. Zhu et al. (2010) Vernadsky V.I., 1965. The chemical structure of the Bakhtin P.U., 1969. Studies of physico-mechanical and Earth's biosphere and its environment. Moscow Kurtzman et al., 2011). Kodamaea ohmeri, reported a K. ohmeri strain isolated from technological properties of the main types of soils of Science, 352 p. formerly known as Pichia ohmeri, is isolated honeybee hives and from osmotic natural the USSR. M. Kolos. 271 p. Viter A.F., 1969. Effect of varying soil density on mostly from food industry being involved in environment able to produce high yields of D- Barber S.A., 1988. The bioavailability of nutrients in the fertilizer efficiency and yield. // Scientific notes of fermentation of pickled products (Vivas et al., arabitol from glucose. soil. Mechanical approach. M.: Agropromizdat. 376 the Belotserkovsky Agricultural Institute, vol. 15, 2016). This species was also reported to be The present study focuses on the preliminary p. Part 2, 117-1149. Berezin P.N., Voronin A.D., Shein E.V., 1985. The main Zubkova T.A., 1998. About the nature of mechanical found in sand, marine environment and part of identification of a new strain K. ohmeri strain parameters and methods for quantitative assessment strength of absolutely dry soil aggregates. Pedology. the microbiota of different living animals (CMGB-ST19) isolated from jam roses (Rosa of the soil structure. Pedology, No. 10, 58-68. No. 3, 291-290. (Chakrabarti et al., 2014). Although some damascena) petals and on the investigation of Bulygin S.Yu., 1993. Quantitative assessment of the Westman W.E., 1978. Measuring the inertia and strains of K. ohmeri are known as being its biotechnological potential. erosion hazard of soils. Pedology, No. 3, 74-79. resilience of ecosystems. Vol. 8, No. 11, 705-710. responsible for different types of opportunistic Dokuchaev V.V., 1949. Selected Works. Moscow. Farm, Tom. 2, 426 p. infections there are many studies regarding its 77 MATERIALS AND METHODS tubes with 50 mM glucose. For the negative by a final extension 5 min at 72°C (Biometra T Antimicrobial activity control no carbon source was added. Gradient Thermocycler). The strain CMGB-ST19 was tested for Biological material The ability to assimilate nitrogen sources was The amplicons were digested with 4 restriction antimicrobial activity against potential The yeast strain CMGB-ST19 was isolated tested using a similar protocol. The 10X Yeast endonucleases: Cfo I (5’-GCG/C-3’), Hinf I pathogenic and pathogenic Candida and from the surface of jam roses (Rosa Carbon Base (YCB) (Sigma Aldrich) medium (5’-G/ANTC-3’), Hae III (5’-GG/CC-3’), Msp Saccharomyces strains: Candida albicans damascena) petals from Bucharest Botanical was supplemented with 1% stock solution: I (5’-C/CGG-3’) (10 U/µl, Promega). The ATCC 10231; C. albicans CMGB-Y1 C. Garden (Romania) and maintained in a Revco 7.8% KNO3, 2.6% NaNO2, 5.6% L-lysine or restriction fragments were observed by gel parapsilosis CMGB 79; C. parapsilosis CBS LegaciTM Refrigeration System (Copeland, 5.08% (NH4)2SO4. electrophoresis (1.7% agarose, TBE 0.5X). The 604; C. parapsilosis CMGB-Y3; C. catenulata UK) at -70%, on Yeast Peptone Glucose (YPG) The strain CMGB-ST19 was assessed for size of the amplicons and restriction fragments CMGB-Y7; C.