AlicsivEs FISHERIES AND MARINE SERVICE Translation Series No. 3465 Lipid contents of plasma and liver of cultured salmonoid fish by Nobukazu Shibata, Toyosuke Kinumaki, and Hiroshi Ichimura Original title: Yoshoku Masurui ni okeru kessho oyobi kanzo no shisitzu ganryo ni tsuite From: Tokaiku Suisan Kenkyusho Kenkyu Hokoku (78): 17-21, 1974 Translated by the Translation Bureau(AT) Multilingual Services Division Department of the Secretary of State of Canada Department of the Environment Fisheries and Marine Service Halifax Laboratory Halifax N.S. 1975 10 pages typescript DEPARTMENT OF THE SECRETARY OF STATE ete, SECRÉTARIAT D'ÉTAT TRANSLATION BUREAU BUREAU DES TRADUCTIONS ix MULTILINGUAL SERVICES meel> DIVISION DES SERVICES CANADA DIVISION MULTILINGUES TRANSLATED FROM - TRADUCTION DE INTO - EN Japanese English AUTHOR - AUTEUR Nobukazu Shibata, Toyosuke Kinumaki and Hiroshi Ichimura TITLE IN ENGLISH - TITRE ANGLAIS Lipid contents of plasma and liver of cultured salmonoid fish TITLE IN FOREIGN LANGUAGE (TRANSLITERATE FOREIGN CHARACTERS) TITRE EN LANGUE ÉTRANGÉRE (TRANSCRIRE EN CARACTÈRES ROMAINS) Yôshoku Masurui ni okeru kessto oyobi kanzd no shisitzu gan1Y0 ni tsuite REFERENCE IN FOREIGN LANGUAGE (NAME OF BOOK OR PUBLICATION) IN FULL. TRANSLITERATE FOREIGN CHARACTERS. RÉFÉRENCE EN LANGUE ÉTRANGÉRE (NOM DU LIVRE OU PUBLICATION), AU COMPLET, TRANSCRIRE EN CARACTÈRES ROMAINS. Tokai Suisan Kenkyu Hokoku REFERENCE IN ENGLISH - RÉFÉRENCE EN ANGLAIS Bull. Tokai Reg. Fish. Res. Lab. PAGE NUMBERS IN ORIGINAL PUBLISHER- ÉDITEUR DATE OF PUBLICATION NUMÉROS DES PAGES DANS DATE DE PUBLICATION L'ORIGINAL 17-21 YEAR ISSUE NO. VOLUME NUMÉRO PAGES PLACE OF PUBLICATION ANNÉE NUMBER OF TYPED LIEU DE PUBLICATION NOMBRE DE PAGES DACTYLOGRAPHIÉES Japan 78 1 0 REQUESTING DEPARTMENT TRANSLATION BUREAU NO. Environment 753356 MINISTRE-CLIENT NOTRE DOSSIER N° BRANCH OR DIVISION Fisheries Service TRANSLATOR (INITIALS) AT DIRECTION OU DIVISION TRADUCTEUR (INITIALES) PERSON REQUESTING T. Reid DEMANDÉ PAR Allan JUN 1 7 1975 YOUR NUMBER VOTRE DOSSIER N 0 UNEDITED TRANSLATION DATE OF REQUEST DATE DE LA DEMANDE May 15, 1973 For information only TRADUCTION NON REVISEE information seulement SO5-20040.6 (REV. 2/68) 7030.21.029-5333 • 'DEPARTMENT OF THE SECRETARY OF STATE SECRÉTARIAT D'ÉTAT TRANSLATION BUREAU BUREAU DES TRADUCTIONS MULTILINGUAL SERVICES DIVISION DES SERVICES DiVISION CANADA MULTILINGUES CLIENTS NO. DEPARTMENT DI VISI ON/BRANCH CITY NO DU CLIENT MINISTÉRE • . DIVISION/DIRECTION VILLE Environment Fisheries Service Ottawa. BUREAU NO. LANGUAGE TRANSLATOR (INITIALS) NO DU BUREAU LANGUE TRADUCTEUR ( INI TI ALES) 753356 Japanese AT JUN 1 7 1975 Bull. Tokal Reg. Fish. Res. Lab., No. 78. June, 1974 Lipid .Contents of Plasma and Liver of Cultured Salmonoid Fish Nobukazu SHIBATA, Toyosuke KiNummu, and Hiroshi ICHIMURA Abetract: This work on the lipid contents of plasma and liver of cultured salmonoids, especially during spawning season, was carried out to get an informatiGn on the physiological and nutritional conditions of fish. Three different species of salmonoids were used in the present experiments, and were sampled to 'determine the lipid contents on September 22 and December 1. They were rainbow trout (Salmo gairdneri iricleus), cherry salmon (Oncorhynchus masou masou) and hime salmon (Oncorhynchus me*: henerlyi) u.à which were raised at Nikko Branch Station, Fresh Water Fisheries Research Laboratory. 2 tn The results obtained were summarised as follows: • C 1^..• -2 1) In Oncorhynchus 5sh, a certain difference in the plasma triglyceride content and December fish, but not in rainbow trout. • 0 was recognized between September fish Z oC Z0 .0 2) In all species used in this experiment, September fish were higher in the hepatic z triglyceride and cholesterol contents and the plasma cholesterol content than December fish. E ;Ls 3) It seemed that matured Oncorhynchus fish was lower in the hepatic triglyceride '6 'o4' e content than that in the immatured at the spal.vning period. ILI • 4) The corelation between the triglyceride contents of liver and that of plasma o was observed only on Oncorhynchus fish. 5) Seasonal fluctuation of the plasma free fatty acid content was observed on dr rainbow trout and cherry salmon. The success of fish culture lies in the understanding of the nutritional requirement and physiological conditions of fish. The present knowledge concerning the nutritional requirement and physiological condition is insufficient'. This necessitates further investigation. In our previous report (1), lipid content Apr. 15, 1974 received, results of Tokal Reg. Fish. Res. Lab. A no. 542. $0$200-I 0-SI -n.r,nTrcr-r,m 2 of pla.sma tc;a.s determined. Ses.sonal fluctuation of various lipid compositions and theirvasiation due to changes. in physiolopAcal con0ition were disr-.ussed. The rel.a.ts onship between the plasma triglyceride content and the maturity of fish was reported. This .paper investigates the relationship between maturity and tri- glyceride contents of plas^-^a and liver using rainbow trout (Salmon gairdneri irideus), cherry salmon (Oncorhynchus masou rasou) and hime salmon ( Oncorhynchus nerka kenerlyi) as experimental fishes especially before and after spawning period. A com- parative study on different species of fish is also carr=.ed out. Furthermore, the cholesterol content and free fatty acid content of plasma as well as cholesterol content of liver are investi- gated. Exnerivental Nethod Ex erimental material: The experimental flshes, i.e. rainbow trout, cherry salmon and hime salmon, were provided by Nikko Branch Station, Fres+.i Water Fisheries Research Laboratory on Septe.mber 22 and December 1, 1972. The average body length and body weight of 2 to 3-year-old fish are as follows: rainbow trout, 35.2 cm, 788 g; cherry salmon, 23.6 cm, 232 g; hime salmon, 27.3 cm, 360 g. Commercial pelletsfor rainbow trout smolt (Oriental. yeast, manufacture^? by K.K., No. 3) together ,tr i t h S% f i s h c u 1 t u re . f e e d i ng o i were prepared to be used as feed. Everyda.y, an amount of feed equivalent to about 1% of the body 7reip•ht t^a.s a•iven to fish unt'l samr)linrP,. Feeding was then ^y,tn•-LN.wrya.m A^ tte-k...^tl y 1„rlvx.ryii .:v^l:^.^.'iw•+:s..xswt<^ .•+!eaF:.+^^:«m^•^•AT'...w.sR.^!rm.rw-_.....-.e......-.T-+..--..^_.^ 3 stopped for two days and the fish were used f -)r experiment. The fish were.. bled under anesthesia with MS - 222 from the caudal vein or artery. The blood was immediately centrifuged and plasma was separated. After ble-ding, the liver WRS removed immedintely and freezed in acetone-dry ice. The plasma o or liver was stored at 20 C until lipid content determination. Lipid analysis: The liver was homogenized with 10 times its volume of water while plasma was used as such for determinations of triglyceride, aholesterol and free fatty acid according to the methods reported previously (1). Exper1mental Results and Discussion The immature group was separated from the mature one during sampling. Fig. 1 shows the average values of experimental results for male and female respectively. In certain batches of these experiments, the experimental sample was insuffielent. Unless the standard deviation was indicated, the results were ob- tained with 1 or 2 individuals only. These results can only be used as references. Plasma components: Triclyceride: Except for the plasma triglyceride content in fenale rainbow tout of the September sample slightly hipeher than that in. male rainbow trout, there is.no conspinuous difference between the September and the December sample (Fig. 1). This Is alost identical to the results of the author's previous re- port (1). In the case of cherry salmon, however, the December fish 4 Rainbow Cherry Flime Species trout salmon salmon Gonadal Immature Mature Immature Mature Immature Mature state â ci p Q â d â ^ 4) ^ 0 Sampling 4) ^  v^  month an u) tn  v^ A v^ rN og P, d lb Fig. 1. Comparison of average lipid contents of each plasma and liver among 3 species of salmonoid fishes. The vertical lines indicate standard error. [:::] : 8 M :9 5 has low trin;lyceride content whether in the mature group or in the Immature group. The male fish tends to have a lower value than fish. Furthermore, *U- e mature female 1-0111e salmon of the female December samole is also observd to have luite low values. As to the male hire salmon of the September sample, the mature 7rouoires a loer value than the immature grOup. Cholesterol: Of all these 3 species of salmonoid fishes, the Decemoer fish tends to have lower value than the September fish (Fig. 1). This agrees with the author's previous report (1) that the plasma cholesterol content of rainbow trout gradually decreases from the summer to winter. It was suggested that the plasma cholesterol - content Is influenced by the amount of choles- terol in the feed (2). The reason for the decrease of plasma cholesterol content from September to December may be attributed to the reduction of the feed Intake before spawning. On the other hand, it was reported that cholesterol plays an Impor- tant role in the generation of egg (3). Before eggs are produced, it is necessary for the ovary to store cholesterol. Plasma is .mobilized. It is possible that the choleSterol content IS thus lowered. Thse points can not be clarified in this paper. As shown in Fig. 1, the plasma cholesterol level decreases in the order; rainbow trout > hime salmon >cherry salmon. How- ever, it Is not clear whether this is due to the difference In fish s)ecies OP the difference in body weight. Pree fatty acid: The free fatty acid content of plasma of rainbow trout is similar to that in th ,, previous report (1). 6 The '')eceber rish ts shown to 1-ive hiph free fatty ad content.