2013 ( 6) ( 35 ) _ Tishreen University Journal for Research and Scientific Studies - Biological Sciences Series Vol. (35) No. (6) 2013 Salvia viridis L. (Lamiaceae )+ , * )* * 34 1 2 0 ** 6 4 + *** 7 ( ( 2013 / 7 / 18 3 ;2 . 2012 / 10 / 31 8 9 ) ­>= /. ,- + ()* ' & Salvia viridis L. ? 1 :> 89 9 1 <= ; 9 - : 89 7' 6 : 4 2 23 2 1 0 . AD 0D C 4 >B <= @A 9 = @/ 7 GC/MS G = 1 = I G 3 7 @ HF G 2 : 3A 7F ­= 1 1 ­/4 - 7 N/ ' & ( 0= C 7 H A . 7H4 1 7 4 1 7 1 7 C . 3A 7F R 9 R - 8 R( )D @ 6) ) *) : * . A 2CD A A 3 B A @ * . A 2CD A A A + 4 2 A ; 4E 2 ** . A 2CD A A A + 4 2 A ; 4E 2 *** 113 123 1- (Lamiaceae )@ 6) *) 2 Salvia viridis L. 2013 ( 6) ( 35 ) _ Tishreen University Journal for Research and Scientific Studies - Biological Sciences Series Vol. (35) No. (6) 2013 Morphological and Chemical study on Salvia viridis L. ( Lamiaceae ) . Dr. Ahmad Kara-Ali * Dr. Abeer Sultan ** Salma Deebo *** (Received 31 / 10 / 2012. Accepted 18 / 7 /2013 ) ABSTRACT A morphological , anatomical and chemical study was carried out on Salvia viridis L. (Lamiaceae ) . The results showed presence of two types of trichomes : - Glandular hairs with four or eight celled globular heads or with oval heads . - Eglandular hairs which are unicellular or multicellular , in addition to presence of candle shaped eglandular hairs . The palynological study revealed that the pollen grains were prolate and they had six germination lines , exine appeared reticulate . The extraction of volatile oils have been done by dry evaporation using GC/MS technique , chromatographic analysis of these extracts have showed the presence of complex of carboxylic acids , hydrocarbons , terpenes , esters and aldehydes . Key words : Lamiaceae , Salvia , Trichomes , Pollen , Essential oils . *Assistant prof , High Institute of Marine Research , Tishreen University , Lattakia , Syria . ** Academic Assistant , Department of Botany, Faculty of Science , Tishreen University, Lattakia, Syria . *** Academic Assistant, Department of Botany , Faculty of Science, Tishreen University, Lattakia, Syria . 114 Tishreen University Journal. Bio. Sciences Series 2013 ( )6 A ( 35 ) A 2 D A :=F 7 6) Lamiaceae @ 6) *) , ( 9 - ) salvia ,L. W 8 Dicotyledones ) G) 7; I* ( 7[ I* Asteridae 7 I* 7/ Lamiales Magnoliophyta (Angiospermes ) . (Cronquist,1981) . ' 7)[ AD ¯3 + 170 , + - 3200 / Labiatae ) )D (@ 6) *) > 4 9 8 4 l/ 2 3 ­4 / ­/ G3 F F C A C>A 2 ,- @<- @ 3 3A 7F 6 *) m'H 7 2 . 3[ C-9 H9 9 1 A3 7' 6 3A 7F p CA> @- ¯ o =9 nH 1 3 ' 2/4 ­A ¯ [ 4 2> m'H p Pikrosalvin @ :/ salvia 8 9 . ( 2005 1>G 2 ) . s @A p 9 NA @ => - R @ 6) *) 4 8 Salvia L. R W 7 0; t Salvia 8 4 + + F A . N/< + G I9 > ' & A . (özdemir et al . , 2009) . C 23 C + - 47 1 + - 90 A 9 A) @ / 2 ' & I* = I N + Salvia W 7 @F , : >= / - 12 2 ;9 7 ) C> A :y 1 > /x + A 1 1 1 2 =t s ) Salvia 8 p + -+ 35 / . : 3A 7F 2 m/ @F 7 C 2;<; 1 R ) ( + A + - 32 0 G (1983) Mouterde 9 (1991 . I* (2011) I= s s'< N/ ) G) 7; ( ) 2 9 ­A S.viridis .C 2 Salvia 8 2 9. ) 7 2/ Salvia (Flora of Libya , 1985) 8 + A + - 32 ) 7 */ (Davis , 1982) . ( + A + - 86 0D9 nH 1 @ Salvia W 8 7 2 / / Salvia viridis L. A 1 1 G- ) 7s :/ :' 7 H @ ; pD 0D 1 7G 7)* ,- -+ /> @- . (özdemir et al . , 2009) . 9 1 >= 1 > 7s 7' 9 7s n ? 0D9 nH + Salvia W 8 2 9 10 2 4 HF9 2- Iy (1998) : A ' 7' @ ) @- (s 1 30R15 (3 D- 7 : A } / H 1 S.viridis C 2 2 4 , HF HF4 1 C/ D= > 5R2 C3 GA 3 ¯ 4 1 2 ) s 7s . ) 1 1 40R10 (-) 1/ 7 29 2 4 }D-4 2- (1985) 9 2 HF4 1 GA 3 > ¯ 4 1 ) 9 ) s ¯ 1 6. ­9 AD ,3[ 8~ 03 IA> (3 2)D ' 1 AD ,3[ 8~ . HF9 6R4 2 2 . ) ( 1 2 / 115 123 1- (Lamiaceae )@ 6) *) 2 Salvia viridis L. I* (1753) ( 0 ,9 ,/ @- 7 S.horminum W S.viridis W 7 7 - : Annual clary sage . H 7 pD ? . 2*) 2- S.horminum S.viridis :H G0 G0 2 2 F ¯G/ (A 29 2 :' 2 1 3A 3 7 @ 2 s<3+ ٕ 24 * l/ 'H - s G 0=. D / 2 / D? p S.viridis , l/ IC .C ( H9 / ( : 3A 7F ­<= 1 ( ) A3 1 ( ) :1 ¯ 6 / l/ A CA = A @/ - s = l/ .2 D A A3 1 D 7 (- 7 9 1 s'< N/ A * s 2 7 p : : 1 1 ¯ : )= >- D 7)* 7 ' & + Gs + - + )* 0 7 I* . > @ @ = @ 2A @ ' ( l 1 ' 1 7< 1 7< ) @ HF 1 s 0 + s 2 D- 3 - 1 s @ 3 A = F 4 I= A9 7 s .@ ' >-4 2 >- :6 1 1 CD 7/ 1 = C<6 7F 1 CD l/ 2 > C 7 p3 7/ 7 7/ (*/ @ (3[ ; 1 F 7 ­/ 2 @ 3s )N F / D ,- 8 C n' B 3= 03 1 ? 3G 2 / ) p3 / 2 D- A9 7 s . @ D > C .3 0 ( = I<[ =; 1 3= 2 1 (> - : : 7 @ HF ) s 3 : 3A 7F 8 .( GA 4 7 D/ [ 2 /. ,- IN 7 p R1 .7 s 2- @ HF ) s 7* R2 .8D AD9 2- A oC IN 2 7)) R3 I G 3 3A 7F ­<= FC C : 3A 7F ­= @ 7/3 R4 (Nickerson apparatus ) . = 116 Tishreen University Journal. Bio. Sciences Series 2013 ( )6 A ( 35 ) A 2 D A : G 3 m'H ­= }C ,- p> / p ( H 6 9 HF9 9 ¯ 9 ) A3G 9 /3 @ 3= 7F 0* 1 *= 7); 3 (); l/ , 7F m = 0/ 1 26 - 1 @ D H/9 1 2- C ) // (7F @* (A 2- ­= 3 + ; ,-4 3 7F o =4 1 3 ¯ )3 ' & 2 ; 0s4 @ 3 7F ­<= ? . G; 7F @ = ? s H 2 ) C ;~ ? 7F ­<= / 6+ G 3 m'H = ( 1993 1 0H - ) . ( )) 9 F3 HF4 ­<= ¯ 4 ­<= = C9 1 @ / :>A 0/ 0/ = }9 @ 2 ­= : 3A 7F p R5 . 3 2 ­= < * 7 I) (diethyl ether B 0 ; ) } A GC/MS 3 3 F[ 6 G : 3A 7F - 7/ R6 . (Kalina et al. , 2003) . @ 3 3A 7F 0/ } 4 G 3 3 5890 Hewlett – Packard F[ 6 FC = 0.25m 2 3 0,32 mm = m 3s HPR5 : AD - = 6972 0 30m . (3 :: / = 7 0* p @ / 1 2ml / min 0A ) C F6 (n / 3 ¯ 250 C ◌ 2s/ @ / 70 ev. D 2 130C° }3s4 - Ion source 230C° 7B 2 = 3 )3 p A 7 2 I34 G3 Nist 98 . Wiley :2 J : J D 0< D / D- 7 H S.viridis 7 29 @ 7 > 9 m 1 50R15 2 (-) 1 / D? p 1 ¯ 3 } ,- (1 0D ) . 2 F/ ; 1 9 3D : CD 2 @ @ ) HF ); ( 2 0D ) . s + /9 @ ; ; ' 1 : 7 ' ( ( C ) s 7s 7' 1 - ) 2 s @ ) 2 s 1 p3G A A> s ¯ ( 30D ) + < + N 7 3A 2 ) 117 123 1- (Lamiaceae )@ 6) *) 2 Salvia viridis L. 1 1 8 D= 1 + Gs + / 7' 1 3 > , > 1 GA 1 3 s *G 03 ¯- 7' @ C ) 1 }* 0G /. ,- p> 1 > ); 9 H (4 0D ) . 70R30 2 s 03 1 ,- C 6R4 @G- 0 - N/< . s 3 Dichasia AD ; @ ) 7 0D ,- HF4 } 0D ,- HFB l/ 7< } G @ HF ) C . 0G 0D 7G p> N+ HF9 3 2 = 1 15 C3 + *s D 7' ) ;= N @/ @ [* @ HF (5 0D ) : HyF 9 . @ 0D / @ [* s 7< 8= 2 2 1 @ *s 3 ); > 9 m 7 (3 1 + 3 FF/ 9 8~ W . }4 C ,/ / G l<; ,G 2/ 2 0D }4 ,-9 2<*) C 2; / ( ( 6 0D ) . ¯ + G*< 0) 4 / + / ¯[ ;B - 8~ => , (C 0*) 6 2 ­9 }9 0D / 7< 8= 2 I~ :)D W 1 1 = 2 @ 1 + 3 C>A ,- 2G3 2/ 2 2 ¯6 ) 2 - : 2)D ) 2 ,) ( 70D ) = , C 2 F 2/ G 2 C= 2>/ .0) 4 2 @ 2 4 , C ,3 F / 7< l<; 2 9 (- ' ) ( s ­9 2 ' @ 3= 1 2 ¯ 2 2 I~ ' W 9(. 9(. 0D )2 9 ­ - 3= (- )*9 2 ' 2(3 03 [* (80D ) ? 6 2 2 2 s 2> C 0 2/ 2 2- @ - :- ­ 2 l~ 2 W ­ A (10 0D ) / D @/ > C 0 R }' F / R 7 / p 9 . + G = 03 IA> (3 H/9 @ HF = 2F ) C 2 C 2 ­9 03 s .@ HF F 9 ( 12 0D ) 2 ( 11 0D )­ 0) 9 @ Discus 6 .@/ @ ' @ ; 0 1 8~ 2> @ ( 7F )7 ; p 9 2 )y @ ; W C*/ o . 1.5 C> - 3 C3 ( 13 0D )2 2 7' 3 @ [* @ ' W 3 ( ,- C >9 : CN ( ,- 2 H 33= ;<; 33= C9 2 > @ . (14 0D ) (Flora of Libya,1985 ; 7 G D 7 p ¯ D 7)* m'H 0A oFA 3 7<=? ­A p 2011) 1 I= & 1998 1 : A (1 Davis,1982) .7 F 9 A9 (1) 0 > . S.viridis / L M0 0 (1) : ; @ ' ­ - @ ' 03 03 8~ 03 @ HF 03 s 03 7 ) 1.5 3 2 7 15 70 R 30 500 R 150 118 Tishreen University Journal. Bio. Sciences Series 2013 ( )6 A ( 35 ) A 2 D A S.viridis 4 N (1) : ; C (2) : ; P( P( ) ( )0 74E A P R E A 0 F F (3) : ; 119 123 1- (Lamiaceae )@ 6) *) 2 Salvia viridis L. S( S( ) (P) ( )0 S.viridis 2 (4) : ; 2 A S A 2 3* H A P A 2 U H A 0 P( P( ) ( )0 74E A P R E A 0 + (5) : ; 120 Tishreen University Journal. Bio. Sciences Series 2013 ( )6 A ( 35 ) A 2 D A A * M (7) : ; ¯ 74 3 @X (6) : ; ت ا ر ا ط أور ط ا داة X25 A : ( 9) ; X25 A + 6> 3* F 74 E : ( 8) ; أور رة ر و ا س X15 A ­ 3 3_ 4 `6F : ( 10 ) ; ا ض ا ا د X20 A ­ ;*0 a : ( 11 ) ; 121 123 1- (Lamiaceae )@ 6) *) 2 Salvia viridis L.