ISSN 1510-7809 LOTUS NEWSLETTER 2007 Volume 37, Number 2 Editor: M. Rebuffo INSTITUTO NACIONAL DE INVESTIGACION AGROPECUARIA Editor: M. Rebuffo INSTITUTO NACIONAL DE INVESTIGACION AGROPECUARIA Editorial Office INIA La Estanzuela This Newsletter consists of informal Colonia, Uruguay reports which are presented to further Phone: +598-574-8000 the exchange of ideas and information Email: [email protected] between research workers. Fax No.: +598-574-8012 Consequently the data presented here Web: http://www.inia.org.uy/sitios/lnl/ are not to be used in publications without the consent of the authors. Images are copyright of the authors, and their reproduction is strictly prohibited without their consent. Front cover: Juan Ramón Acebes Ginovés and Felicia Oliva Tejera spoke about the endemic Lotus species of the Canary Islands (pp. 14-15) at the Workshop: Lotus as a model legume and a sustainable alternative for marginal land reclamation (Botanic Garden of the University of Valencia, Spain, September 6-7 2007). The photograph on the front cover shows flowers of Lotus berthelotii, one of the species from the Canary Islands. The picture was recorded at the Jardin Mundani, Mallorca, Islas Baleares, Spain, and authorized by its publication at Lotus Newsletter 37(2) by Juan Bibiloni Pou. The opinions in this publication are those of the authors and not necessarily those of the Lotus Newsletter. The designations employed and the presentation of the material in this publication do not imply the expression of any opinion whatsoever on the part of the Newsletter concerning the legal status of any country, territory, city, or area, or of its authorities, or concerning the delimitation of its frontiers or boundaries. Where trade names are used this does not constitute endorsement of or discrimination against any product by the Newsletter. Lotus Newsletter (2007) Volume 37 (2), i-iv. SEPTEMBER 6-7, 2007. VALENCIA, SPAIN i ii ORGANIZADORES DEL WORKSHOP: WORKSHOP ORGANIZERS: DR. JUAN SANJUÁN (EEZ-CSIC) DR. OSCAR A. RUIZ (UNIVERSITAT DE VALENCIA) SECRETARÍA DEL WORKSHOP: WORKSHOP SECRETARIAT: ROSA FRÁPOLLI (EEZ-CSIC) ENTIDADES COLABORADORAS: UNDER THE SPONSORSHIP OF: MINISTERIO DE EDUCACION Y CIENCIA Cátedra UNESCO de Estudios sobre el Desarrollo CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS ESTACION EXPERIMENTAL DEL ZAIDIN iii Programme Thursday, September 6 8:30-9:00 Welcome. Manuel Costa Talens, Vicerector de Relacions Internacionals i Cooperació, Universitat de València 9:00-9:30 Opening: Arnoldo Santos (Tenerife, Spain) A global survey of genus Lotus (Loteae-Fabaceae) Session 1: Lotus spp. Genetic and Genomic Resources 9:45-10:15 Jens Stougaard (Aarhus, Denmark) Lotus genetics and genomics: Resources and approaches 10:30-11:00 Coffee Break 11:00-11:30 Mónica Rebuffo (Colonia, Uruguay) Genetic resources of forage legumes for agriculture-pastoral system in Uruguay 11:45-12:15 Hernán Acuña (Chillán, Chile) Genetic resources and agropastoral systems in Chile 12:30-13:00 Miguel Dall’Agnol (Porto Alegre, Brasil) Legume Utilization in Grazing Systems in Southern Brazil 13:15-15:00 Lunch Session 2: Taxonomy and Ecophysiology 15:00-15:30 Francisco Escaray (Chascomús, Argentina) Biodiversity of Lotus spp. in Devesa of l`Albufera (Valencia, Spain) 15:45-16:30 Josep Roselló Picornell (Valencia, Spain) Evolution in insular Mediterranean Lotus. What we know and what should be known 16:45-17:15 Coffee Break 17:15-17:45 Juan R. Acebes (Tenerife, Spain) Lotus endémicos de Canarias, situación y potencial aprovechamiento. 18:00-18:30 Rolando León (Argentina) Río de la Plata grasslands and Lotus tenuis 18:45-19:45 Round Table. Chair Oscar Ruiz iv Friday, September 7 Session 3: Biochemistry and Biotechnology 8:30-9:00 Carmen López-Valiente (Valencia, Spain) Germinative response of Lotus creticus to different temperatures and salinity conditions 9:00-9:30 Antonio Márquez (Sevilla, Spain) Primary and secondary nitrogen assimilation in Lotus japonicus and the relationship with drought stress 9:45-10:15 Oscar Ruiz (Chascomús, Argentina) Lotus tenuis as keystone species for the Salado River Basin (Argentine) 10:30.11:00 Coffee Break 11:00-11:30 Carmen Lluch (Granada, Spain) Saline stress tolerance in legumes 11:45-12:15 Esther González (Pamplona, Spain) Legume carbon metabolism under stress: Lotus japonicus features 12:30-13:00 Francisco Escaray (Chascomús, Argentina) Condensed tannins in the Genus Lotus 13:00-13:30 Francesco Paolocci (Perugia, Italy) Genetic manipulation of condensed tannin biosynthesis in Lotus spp 13:30-15:00 Lunch Session 4: Microbiology 15:00-15:30 Milagros León Barrios (Tenerife, Spain) Genetic diversity in the rhizobia isolated from endemic Lotus to the Canary Islands 15:45-16:15 Isabel Videira e Castro (Oeiras, Portugal) Use of Lotus/Rhizobium Symbiosis in Regeneration of Polluted Soils 16:30-17:00 Coffee Break 17:00-17-30 Nuria Ferrol (Granada, Spain) Legumes as model plants to study nutrient transport processes in arbuscular mycorrhiza 17:45-18:45 Round Table, Chair Juan Sanjuán 18:45-19:00 Conclusions Lotus Newsletter. (2007). Volume 37. Number 2. Contents Workshop: Lotus as a model legume and a sustainable alternative for marginal land reclamation Program i A. SANTOS. A global survey of genus Lotus (Loteae-Fabaceae). [Una visión global del género Lotus (Loteae, Fabaceae)] 52 Session 1: Lotus spp. Genetic and Genomic Resources [Recursos Genéticos y Genómicos en Lotus spp.]. J. STOUGAARD, N. SANDAL, N. JØRGENSEN, S. DAM, G. NAUTROP, J. FREDSLUND, B.K. HOUGAARD, S. RADUTOIU, L. TIRICHINE, L.H. MADSEN, E.B. MADSEN, Y. KEISUKE, P. ROMERO, A. JURKIEWICH, A. ALBREKTSEN, E.M.H. QUISTGAARD and E. FUKAI. Lotus genetics and genomics: Resources and approaches. 54 M. REBUFFO, R. ZARZA, O. BORSANI, E. CASARETTO, A. MESSA, M.M. SAINZ, R. SALDIAS, R. ALZUGARAY, F. CONDÓN, P. DIAZ, J. MONZA, D. RISSO, M. BEMHAJA, R. BERMUDEZ, W. AYALA, N. ALTIER, M. ZARZA. Genetic resources of forage legumes for agriculture-pastoral system in Uruguay. [Recursos genéticos de leguminosas forrajeras para sistema agro- pastoriles en Uruguay] 56 H. ACUÑA. Genetic resources and agropastoral systems in Chile. [Recursos genéticos y sistemas agro-pastoriles en Chile.] 57 M. DALL’AGNOL and S.M.S. BASSO. Legume Utilization in Grazing Systems in Southern Brazil. [Utilização de Leguminosas em Sistemas Pastoris do Sul do Brasil.] 59 Session 2: Taxonomy and Ecophysiology [Taxonomía y Ecofisiología]. F. ESCARAY, A. SCAMBATO, P. COLLADO ROSIQUE, A. VIZCAINO MATARREDONA, J. ROSELLO PICORNELL, M. ROSATO, C. ANTOLÍN TOMÁS, P. CARRASCO SORLI and O.A. RUIZ. Biodiversity of Lotus spp. in Devesa of l`Albufera (Valencia, Spain). [Biodiversidad de Lotus spp. En la Devesa de l`Albufera (Valencia, España)] 62 J.A. ROSELLÓ. Evolution in insular Mediterranean Lotus. What we know and what should be known. [Evolución en Lotus insulares Mediterráneos. Qué conocemos y qué deberíamos conocer] 64 J.R. ACEBES GINOVÉS and FELICIA OLIVA TEJERA. Current status and uses of the endemic Lotus to the Canary Islands. [Lotus endémicos de Canarias, situación y potencial aprovechamiento.] 65 R.J.C. LEÓN, G. STRIKER, P. INSAUSTI and S.B. PERELMAN. Río de la Plata grasslands and Lotus tenuis. [Pastizales del Río de la Plata y Lotus tenuis] 67 Session 3: Biochemistry and Biotechnology [Bioquímica y Biotecnología]. C. LÓPEZ VALIENTE, E. ESTRELLÉS, P. SORIANO and J. PICÓ. Germinative response of Lotus creticus to different temperatures and salinity conditions. [Respuesta germinativa de Lotus creticus a distintas temperaturas y condiciones de salinidad] 69 A.J. MÁRQUEZ, M. BETTI, M. GARCÍA-CALDERÓN, A. CREDALI, P. DÍAZ and J. MONZA. Primary and secondary nitrogen assimilation in Lotus japonicus and the relationship with drought stress. [Asimilación primaria y secundaria de nitrógeno en Lotus japonicus e interrelación con el estrés hídrico] 71 F.L. PIECKENSTAIN, M.J. ESTRELLA, A. SANNAZZARO, A. MENÉNDEZ, V. FRACAROLI, N. CASTAGNO, M. ECHEVERRÍA, J. PESQUEIRA, P. VERTIZ, R. PAZ, M.E. MICIELI, F. ESCARAY, V. BERGOTTINI, S. SCHULMEISTER, P. UCHIYA, B. ROSSO, A. ANDRES and O.A. RUIZ. Lotus tenuis as keystone species for the Salado River Basin (Argentine). [Lotus tenuis como especie clave para la Pampa deprimida del Salado (Argentina).] 74 C. LLUCH, N. TEJERA, J.A. HERRERA-CERVERA, M. LOPEZ, J.R. BARRANCO-GRESA, F.J. PALMA, M. GOZÁLVEZ, C. IRIBARNE, E. MORENO and A. OCAÑA. Saline stress tolerance in legumes. [Tolerancia al estrés salino en leguminosas] 76 E.M. GONZÁLEZ, E. LARRAINZAR, R. LADRERA, C. DE MIGUEL and C. ARRESE-IGOR. Legume carbon metabolism under stress: Lotus japonicus features. [Metabolismo del carbono en leguminosas bajo estrés: características de Lotus japonicus] 78 F. ESCARAY, J. PESQUEIRA, F. DAMIANI, F. PAOLOCCI, P. CARRASCO SORLI and O.A. RUIZ. Condensed tannins in Lotus species under salt stress. [Taninos condensados en especies de Lotus en condiciones de estrés salino] 81 F. PAOLOCCI, S. ARCIONI and F. DAMIANI. Genetic manipulation of condensed tannin biosynthesis in Lotus spp. 84 Lotus Newsletter. 2007. Volume 37. Number 2. Session 4: Microbiology [Microbiología]. M. LEÓN BARRIOS and J. DONATE CORREA. Genetic diversity in the rhizobia isolated from endemic Lotus to the Canary Islands [Diversidad genética de los rizobios aislados de Lotus endémicos de las Islas Canarias] 86 I. VIDEIRA E CASTRO, P. SÁ-PEREIRA, F. SIMÕES, J.A. MATOS and E. FERREIRA. Use of Lotus/Rhizobium Symbiosis in Regeneration of Polluted Soils. [Utilização da Simbiose Lotus/Rhizobium na Regeneração de Solos Poluídos.] 87 N. FERROL. Legumes as model plants to study nutrient transport processes in arbuscular mycorrhiza. [Las leguminosas como plantas modelo para estudiar los procesos de transporte de nutrientes en micorrizas arbusculares.] 89 iv Lotus Newsletter (2007) Volume 37 (2), 52 - 53. Abstract, Workshop held at Valencia, 6-7 September 2007 A global survey of genus Lotus (Loteae-Fabaceae) ARNOLDO SANTOS * Unidad de Botánica-Instituto Canario de Investigaciones Agrarias (ICIA). Jardín de Aclimatación de La Orotava, Calle Retama 2, 38400 Puerto de la Cruz (Tenerife). Canary Islands- Spain.