Nordic Journal of Botany NJB-01778 Tendal, K., Larsen, B., Ørgaard, M. and Pedersen, C. 2018. Recurrent hybridization events between Primula vulgaris, P. veris and P. elatior (Primulaceae, Ericales) challenge the species boundaries: Using molecular markers to re-evaluate morphological identifications. - Nordic Journal of Botany 2018: e01778 Appendix 1. Map of collection locations. The map shows the eastern part of Denmark and a bit of Sweden with a single location in Scania. Most samples were from the Islands Møn and Lolland in the southern Denmark. The label for Kisserup is partly covered by the label for Lejre. Map was drawn using http://www.gpsvisualizer.com/. Appendix 2. List of Primula accessions with precise indication of collection location, flower morph, ITS-type (HaeIII and RsaI-digestion profile) and chloroplast type (trnL-type). The accessions from which the trnL-fragments were sequenced are indicated. Amplified Locatio Geographica for >5 Amplified Sanger Accession Species Morph HaeIII RsaI n l coordinates SSR for trnL sequenced markers Møns Klint 54°57'58.6''N 101 P. ×digenea Thrum A CD Yes P. vulgaris (MK), 12°32'55.4''E Møn MK, 54°57'58.6''N Homo- 102 P. elatior A D Yes P. vulgaris Møn 12°32'55.4''E style MK, 54°57'58.6''N 103 P. elatior Pin D Yes P. elatior Møn 12°32'55.4''E MK, 54°57'58.6''N 104 P. ×digenea Pin A D Yes P. vulgaris Møn 12°32'55.4''E MK, 54°57'58.6''N 105 P. ×digenea Thrum A CD Yes P. vulgaris Møn 12°32'55.4''E MK, 54°57'58.6''N 106 P. ×digenea Thrum A CD Yes P. vulgaris Møn 12°32'55.4''E MK, 54°57'58.6''N 107 P. vulgaris Thrum A C Yes P. vulgaris Møn 12°32'55.4''E MK, 54°57'58.6''N 108 P. elatior A Yes P. vulgaris Møn 12°32'55.4''E MK, 54°57'58.6''N 109 P. ×digenea Pin A CD Yes P. vulgaris Møn 12°32'55.4''E MK, 54°57'58.6''N 110 P. elatior A D Yes P. elatior Møn 12°32'55.4''E MK, 54°57'58.6''N 111 P. elatior Thrum A D Yes P. vulgaris Møn 12°32'55.4''E MK, 54°57'58.6''N 112 P. vulgaris Pin A C Yes P. vulgaris Møn 12°32'55.4''E MK, 54°57'58.6''N 113 P. ×digenea Thrum A CD Yes P. vulgaris Møn 12°32'55.4''E MK, 54°57'58.6''N 114 P. elatior Thrum A CD Yes P. vulgaris Møn 12°32'55.4''E MK, 54°58'16.6''N 201 P. elatior Thrum A D Yes P. elatior Yes Møn 12°32'57.7''E MK, 54°58'16.6''N 202 P. elatior A D Yes P. elatior Møn 12°32'57.7''E MK, 54°58'16.6''N 203 P. elatior Yes P. elatior Møn 12°32'57.7''E MK, 54°58'16.6''N 204 P. elatior A D Yes P. elatior Møn 12°32'57.7''E MK, 54°58'16.6''N 205 P. elatior A D Yes P. elatior Møn 12°32'57.7''E MK, 54°58'16.6''N 206 P. elatior Thrum A D Yes P. elatior Møn 12°32'57.7''E MK, 54°58'16.6''N 207 P. elatior Pin A D Yes P. elatior Møn 12°32'57.7''E MK, 54°58'16.6''N 208 P. vulgaris Thrum A C Yes P. vulgaris Yes Møn 12°32'57.7''E MK, 54°58'16.6''N 209 P. ×digenea Pin A D Yes P. vulgaris Møn 12°32'57.7''E MK, 54°58'16.6''N 210 P. elatior Pin A D Yes P. vulgaris Møn 12°32'57.7''E MK, 54°58'16.6''N 211 P. ×digenea Thrum Yes P. vulgaris Møn 12°32'57.7''E MK, 54°58'16.6''N 212 P. ×digenea Thrum A CD Yes P. vulgaris Møn 12°32'57.7''E MK, 54°58'16.6''N 213 P. vulgaris A C Yes P. vulgaris Møn 12°32'57.7''E MK, 54°58'16.6''N 214 P. vulgaris Thrum A C Yes P. vulgaris Møn 12°32'57.7''E Amplified Geographical for >5 Amplified Sanger Accession Species Location Morph HaeIII RsaI coordinates SSR for trnL sequenced markers MK, Møn 54°58'16.6''N 215 P. elatior Pin A D Yes P. elatior 12°32'57.7''E MK, Møn 54°58'16.6''N 216 P. ×digenea Pin A CD Yes P. vulgaris 12°32'57.7''E MK, Møn 54°58'16.6''N 217 P. ×digenea Yes P. elatior 12°32'57.7''E MK, Møn 54°58'16.6''N 218 P. elatior A D Yes P. elatior 12°32'57.7''E MK, Møn 54°58'15.8''N 219 P. elatior A D Yes P. elatior 12°33'02.0''E MK, Møn 54°58'15.8''N 220 P. vulgaris Thrum A C Yes P. vulgaris 12°33'02.0''E MK, Møn 54°58'15.8''N 221 P. elatior Thrum Yes P. elatior 12°33'02.0''E MK, Møn 54°58'15.8''N 222 P. ×digenea A CD Yes P. elatior 12°33'02.0''E MK, Møn 54°58'15.8''N 223 P. vulgaris A CD Yes P. vulgaris 12°33'02.0''E MK, Møn 54°58'22.6''N 301 P. elatior A D Yes P. elatior 12°33'59.7''E MK, Møn 54°58'22.6''N 302 P. elatior Yes P. elatior 12°33'59.7''E MK, Møn 54°58'22.6''N 303 P. elatior Thrum A D Yes P. elatior 12°33'59.7''E MK, Møn 54°58'22.6''N 304 P. ×digenea Thrum A CD Yes P. elatior 12°33'59.7''E MK, Møn 54°58'22.6''N 305 P. ×digenea Thrum A CD Yes P. elatior 12°33'59.7''E MK, Møn 54°58'22.6''N 306 P. elatior Thrum A D Yes P. elatior 12°33'59.7''E MK, Møn 54°58'22.6''N 307 P. ×digenea Pin A CD Yes P. elatior 12°33'59.7''E MK, Møn 54°58'22.6''N 308 P. ×digenea Pin Yes P. vulgaris 12°33'59.7''E MK, Møn 54°58'22.6''N 309 P. ×digenea Pin A CD Yes P. elatior 12°33'59.7''E MK, Møn 54°58'22.6''N 310 P. vulgaris Pin C Yes P. elatior 12°33'59.7''E MK, Møn 54°58'22.6''N 311 P. vulgaris Thrum A P. vulgaris Yes 12°33'59.7''E MK, Møn 54°58'22.6''N 312 P. ×digenea Thrum A CD Yes P. vulgaris 12°33'59.7''E MK, Møn 54°58'22.6''N 313 P. elatior Thrum A D Yes P. elatior 12°33'59.7''E MK, Møn 54°58'22.6''N 314 P. vulgaris Pin A C Yes P. vulgaris 12°33'59.7''E MK, Møn 54°58'22.6''N 315 P. elatior Thrum A D Yes P. elatior 12°33'59.7''E MK, Møn 54°58'23.1''N 316 P. ×polyantha Thrum B CD Yes P. veris 12°33'02.1''E MK, Møn 54°58'59.9''N 402 P. ×polyantha Thrum B CD Yes P. veris 12°31'51.6''E MK, Møn 54°58'59.9''N 403 P. ×polyantha B CD Yes P. veris 12°31'51.6''E MK, Møn 54°58'59.9''N Homo- 404 P. ×polyantha B Yes P. veris 12°31'51.6''E style MK, Møn 54°58'59.9''N Homo- 405 P. ×polyantha B CD Yes P. veris 12°31'51.6''E style 54°58'59.9''N 406 P. ×polyantha MK, Møn Pin CD Yes P. veris 12°31'51.6''E Amplified Geographical for >5 Amplified Sanger Accession Species Location Morph HaeIII RsaI coordinates SSR for trnL sequenced markers 55°35'46.7"N 501 P. veris Lejre B D Yes P. veris 11°56'06.8"E 55°35'46.7"N 502 P. elatior Lejre P. elatior 11°56'06.8"E Købelev Skov 54°55'55.9"N 503 P. vulgaris Yes (KS), 11°05'45.8"E Lolland KS, 54°55'55.9"N 504 P. vulgaris C Yes P. vulgaris Lolland 11°05'45.8"E KS, 54°55'55.9"N 505 P. vulgaris Lolland 11°05'45.8"E KS, 54°55'55.9"N 506 P. vulgaris A C Yes P. vulgaris Lolland 11°05'45.8"E KS, 54°55'55.9"N 507 P. vulgaris Yes P. veris Lolland 11°05'45.8"E KS, 54°55'55.9"N 508 P. vulgaris A C Yes P. vulgaris Lolland 11°05'45.8"E KS, 54°55'55.9"N 509 P. vulgaris Yes P. vulgaris Lolland 11°05'45.8"E KS, 54°55'55.9"N 510 P. vulgaris Lolland 11°05'45.8"E KS, 54°55'55.9"N 511 P. vulgaris Lolland 11°05'45.8"E KS, 54°55'55.9"N 512 P. vulgaris P. vulgaris Lolland 11°05'45.8"E KS, 54°55'57.4"N 513 P.