Supplementary Table S1. Protein identification parameters Item Value Enzyme Trypsin Max Missed Cleavages 2 Carbamidomethyl (C), Fixed modifications TMT 10 plex (N-term), TMT 10 plex (K) Variable modifications Oxidation (M) Peptide Mass Tolerance ± 20 ppm Fragment Mass Tolerance 0.1Da Database uniprot_rat_36079_20170921.fasta Database pattern Decoy Peptide FDR ≤0.01 The protein ratios are calculated as the median of Protein Quantification only unique peptides of the protein Normalizes all peptide ratios by the median protein Experimental Bias ratio. The median protein ratio should be 1 after the normalization. Supplementary Table S2. List of protein quantification and differential analysis Accession Description HF/C t test p value Cytochrome P450 2C70 OS=Rattus norvegicus GN=Cyp2c70 P19225 1.808583525 9.953E-07 PE=2 SV=1 - [CP270_RAT] Carboxylic ester hydrolase OS=Rattus norvegicus G3V9D8 1.583811454 1.61313E-06 GN=LOC108348093 PE=1 SV=1 - [G3V9D8_RAT] Epoxide hydrolase 1 OS=Rattus norvegicus GN=Ephx1 PE=1 P07687 1.732555816 3.21041E-06 SV=1 - [HYEP_RAT] Cholesterol 7-alpha-monooxygenase OS=Rattus norvegicus P18125 1.398497925 4.77469E-06 GN=Cyp7a1 PE=1 SV=1 - [CP7A1_RAT] Methyltransferase-like protein 7B OS=Rattus norvegicus Q562C4 1.462168835 5.07554E-06 GN=Mettl7b PE=1 SV=1 - [MET7B_RAT] Serum paraoxonase/arylesterase 1 OS=Rattus norvegicus P55159 1.451799589 8.48466E-06 GN=Pon1 PE=1 SV=3 - [PON1_RAT] Estradiol 17-beta-dehydrogenase 11 OS=Rattus norvegicus Q6AYS8 1.652588897 1.39328E-05 GN=Hsd17b11 PE=2 SV=1 - [DHB11_RAT] Peroxisomal bifunctional enzyme OS=Rattus norvegicus P07896 1.221789306 1.92615E-05 GN=Ehhadh PE=1 SV=2 - [ECHP_RAT] Cytochrome P450 3A2 OS=Rattus norvegicus GN=Cyp3a2 P05183 1.588862955 1.98411E-05 PE=1 SV=2 - [CP3A2_RAT] Aldehyde oxidase 1 OS=Rattus norvegicus GN=Aox1 PE=1 F1LRQ1 1.283524072 2.20966E-05 SV=3 - [F1LRQ1_RAT] Receptor expression-enhancing protein 5 OS=Rattus norvegicus B2RZ37 1.539226349 2.31705E-05 GN=Reep5 PE=1 SV=1 - [REEP5_RAT] Carboxylesterase 1D OS=Rattus norvegicus GN=Ces1d PE=1 P16303 1.408036765 2.75019E-05 SV=2 - [CES1D_RAT] Dehydrogenase/reductase (SDR family) member 1 OS=Rattus Q6AXY8 1.536302694 2.83349E-05 norvegicus GN=Dhrs1 PE=1 SV=1 - [Q6AXY8_RAT] Retinal dehydrogenase 1 OS=Rattus norvegicus GN=Aldh1a1 P51647 1.840092428 3.4716E-05 PE=1 SV=3 - [AL1A1_RAT] Carboxylic ester hydrolase OS=Rattus norvegicus GN=Ces2a Q8K3R0 1.419675339 4.56731E-05 PE=2 SV=1 - [Q8K3R0_RAT] Ras-related protein Rab-18 OS=Rattus norvegicus GN=Rab18 Q5EB77 1.384447664 5.65736E-05 PE=2 SV=1 - [RAB18_RAT] Perilipin OS=Rattus norvegicus GN=Plin2 PE=2 SV=1 - Q5U2U5 2.013776015 5.94017E-05 [Q5U2U5_RAT] UDP-glucuronosyltransferase 1-1 OS=Rattus norvegicus Q64550 1.493772264 8.17548E-05 GN=Ugt1a1 PE=1 SV=1 - [UD11_RAT] Acyl-coenzyme A oxidase OS=Rattus norvegicus GN=Acox2 F1LNW3 1.203005945 8.86563E-05 PE=1 SV=1 - [F1LNW3_RAT] Carboxylic ester hydrolase OS=Rattus norvegicus GN=Ces2h Q32Q55 1.240199167 0.000107193 PE=1 SV=1 - [Q32Q55_RAT] Lysosome membrane protein 2 OS=Rattus norvegicus P27615 1.278903221 0.000111769 GN=Scarb2 PE=1 SV=2 - [SCRB2_RAT] Hypoxanthine-guanine phosphoribosyltransferase OS=Rattus P27605 1.2186336 0.000123457 norvegicus GN=Hprt1 PE=1 SV=1 - [HPRT_RAT] UDP-glucuronosyltransferase OS=Rattus norvegicus D3ZLR6 1.225683241 0.000140185 GN=Ugt2b15 PE=1 SV=3 - [D3ZLR6_RAT] UDP-glucuronosyltransferase OS=Rattus norvegicus Q8VD45 1.399046982 0.000144338 GN=Ugt1a5 PE=2 SV=1 - [Q8VD45_RAT] Cytochrome P-450 OS=Rattus norvegicus GN=Cyp3a23/3a1 Q06884 1.511835628 0.000147063 PE=1 SV=1 - [Q06884_RAT] Bile acid-CoA:amino acid N-acyltransferase OS=Rattus Q63276 1.200113485 0.000148178 norvegicus GN=Baat PE=1 SV=2 - [BAAT_RAT] Liver carboxylesterase 4 OS=Rattus norvegicus PE=2 SV=2 - Q64573 1.260627651 0.000159303 [EST4_RAT] Cytochrome P450 2C23 OS=Rattus norvegicus GN=Cyp2c23 P24470 1.318079504 0.000165039 PE=2 SV=2 - [CP2CN_RAT] Glutathione S-transferase, theta 3 OS=Rattus norvegicus D3Z8I7 1.239882511 0.000232724 GN=Gstt3 PE=1 SV=1 - [D3Z8I7_RAT] Aflatoxin B1 aldehyde reductase member 3 OS=Rattus P38918 1.494791392 0.0002348 norvegicus GN=Akr7a3 PE=1 SV=2 - [ARK73_RAT] Cytochrome b5 OS=Rattus norvegicus GN=Cyb5a PE=1 SV=2 P00173 1.227719353 0.000270095 - [CYB5_RAT] Very long-chain acyl-CoA synthetase OS=Rattus norvegicus P97524 1.249642255 0.000280848 GN=Slc27a2 PE=1 SV=1 - [S27A2_RAT] Enoyl-[acyl-carrier-protein] reductase, mitochondrial A0A140TAE6 OS=Rattus norvegicus GN=Mecr PE=1 SV=1 - 1.222638422 0.000332009 [A0A140TAE6_RAT] Cytochrome P450 3A18 OS=Rattus norvegicus GN=Cyp3a18 G3V635 1.372231059 0.000353707 PE=1 SV=1 - [G3V635_RAT] NADPH--cytochrome P450 reductase OS=Rattus norvegicus P00388 1.227318458 0.000406763 GN=Por PE=1 SV=3 - [NCPR_RAT] Glutathione S-transferase alpha-5 OS=Rattus norvegicus P46418 1.676939556 0.000423909 GN=Gsta5 PE=1 SV=2 - [GSTA5_RAT] Methyltransferase like 7A, isoform CRA_b OS=Rattus Q3KRE2 1.362230744 0.000447562 norvegicus GN=Mettl7a PE=1 SV=1 - [Q3KRE2_RAT] CCZ1 homolog B, vacuolar protein-trafficking and biogenesis- A0A140UHX9 associated OS=Rattus norvegicus GN=Ccz1b PE=1 SV=1 - 1.260979136 0.000471004 [A0A140UHX9_RAT] Membrane-associated progesterone receptor component 1 P70580 OS=Rattus norvegicus GN=Pgrmc1 PE=1 SV=3 - 1.250193271 0.00048953 [PGRC1_RAT] Acyl-coenzyme A synthetase ACSM5, mitochondrial Q6AYT9 OS=Rattus norvegicus GN=Acsm5 PE=2 SV=1 - 1.34807686 0.000492078 [ACSM5_RAT] Alcohol dehydrogenase 4 OS=Rattus norvegicus GN=Adh4 A1L128 1.203832432 0.000524367 PE=1 SV=1 - [A1L128_RAT] RGD1563250 (Predicted), isoform CRA_a OS=Rattus D3ZJZ0 1.26805429 0.000604991 norvegicus GN=Tmem205 PE=1 SV=1 - [D3ZJZ0_RAT] Solute carrier organic anion transporter family member 1A4 O35913 OS=Rattus norvegicus GN=Slco1a4 PE=2 SV=1 - 1.316493831 0.000627043 [SO1A4_RAT] Peroxisomal multifunctional enzyme type 2 OS=Rattus P97852 1.212332672 0.00066838 norvegicus GN=Hsd17b4 PE=1 SV=3 - [DHB4_RAT] Myosin, heavy chain 15 OS=Rattus norvegicus GN=Myh15 A0A0G2JY53 1.279298327 0.000676785 PE=3 SV=1 - [A0A0G2JY53_RAT] UDP-glucuronosyltransferase OS=Rattus norvegicus D4A147 1.313522947 0.000686365 GN=Ugt2a3 PE=1 SV=3 - [D4A147_RAT] Translation initiation factor eIF-2B subunit beta OS=Rattus Q62818 2.16653058 0.000718203 norvegicus GN=Eif2b2 PE=2 SV=1 - [EI2BB_RAT] Cathepsin D OS=Rattus norvegicus GN=Ctsd PE=1 SV=1 - P24268 1.277952633 0.000768561 [CATD_RAT] Ectonucleoside triphosphate diphosphohydrolase 5 OS=Rattus F1LPB8 1.211922712 0.000899361 norvegicus GN=Entpd5 PE=1 SV=2 - [F1LPB8_RAT] UDP-glucuronosyltransferase 2B1 OS=Rattus norvegicus P09875 1.410760147 0.000984948 GN=Ugt2b1 PE=2 SV=1 - [UD2B1_RAT] PDZ and LIM domain protein 1 OS=Rattus norvegicus P52944 1.338035136 0.000997626 GN=Pdlim1 PE=1 SV=4 - [PDLI1_RAT] Platelet glycoprotein 4 OS=Rattus norvegicus GN=Cd36 PE=1 A0A096MJ39 1.226873712 0.001019201 SV=1 - [A0A096MJ39_RAT] 3-ketoacyl-CoA thiolase B, peroxisomal OS=Rattus norvegicus P07871 1.728987207 0.001173721 GN=Acaa1b PE=1 SV=2 - [THIKB_RAT] Cysteine dioxygenase type 1 OS=Rattus norvegicus GN=Cdo1 P21816 1.438903572 0.001253103 PE=1 SV=1 - [CDO1_RAT] UDP-glucuronosyltransferase OS=Rattus norvegicus A0A0G2K727 1.209882937 0.001325651 GN=Ugt2b35 PE=1 SV=1 - [A0A0G2K727_RAT] Dimethylaniline monooxygenase [N-oxide-forming] 1 P36365 1.21035083 0.001344383 OS=Rattus norvegicus GN=Fmo1 PE=1 SV=2 - [FMO1_RAT] 2,4-dienoyl CoA reductase 1, mitochondrial, isoform CRA_a G3V734 OS=Rattus norvegicus GN=Decr1 PE=1 SV=1 - 1.20205063 0.001385445 [G3V734_RAT] Palmitoyl-protein thioesterase 1 OS=Rattus norvegicus P45479 1.283496091 0.001414815 GN=Ppt1 PE=1 SV=1 - [PPT1_RAT] Srxn1 protein (Fragment) OS=Rattus norvegicus GN=Srxn1 B3DM86 1.226049465 0.001620823 PE=2 SV=1 - [B3DM86_RAT] Carboxylic ester hydrolase OS=Rattus norvegicus GN=Ces1a D4AA05 1.330073999 0.001718485 PE=1 SV=1 - [D4AA05_RAT] UDP-glucuronosyltransferase OS=Rattus norvegicus P97886 1.290171117 0.001795007 GN=UGT1 PE=2 SV=1 - [P97886_RAT] Prospero homeobox 2 OS=Rattus norvegicus GN=Prox2 PE=4 D3ZHL7 1.558385759 0.001853077 SV=2 - [D3ZHL7_RAT] UDP-glucuronosyltransferase OS=Rattus norvegicus F1LM22 1.228549218 0.002271255 GN=Ugt2b PE=1 SV=2 - [F1LM22_RAT] Sulfotransferase OS=Rattus norvegicus GN=Sult1c2a PE=2 Q569D0 1.284200924 0.002281477 SV=1 - [Q569D0_RAT] Enoyl-CoA delta isomerase 1, mitochondrial OS=Rattus P23965 1.218036059 0.002456794 norvegicus GN=Eci1 PE=1 SV=1 - [ECI1_RAT] N-acetyltransferase 8 OS=Rattus norvegicus GN=Nat8 PE=1 Q9QXT3 1.260794763 0.002624452 SV=1 - [NAT8_RAT] Proteoglycan 4 OS=Rattus norvegicus GN=Prg4 PE=1 SV=2 - F1LRA5 1.275701953 0.002921552 [F1LRA5_RAT] Carboxylic ester hydrolase OS=Rattus norvegicus GN=Ces1d A0A0G2JY66 1.321744902 0.002988524 PE=1 SV=1 - [A0A0G2JY66_RAT] BRISC and BRCA1-A complex member 1 OS=Rattus Q5XIJ6 2.047810457 0.003061915 norvegicus GN=Babam1 PE=1 SV=1 - [BABA1_RAT] Transmembrane protein 116 OS=Rattus norvegicus F1LRF5 1.221267555 0.003105812 GN=Tmem116 PE=4 SV=2 - [F1LRF5_RAT] Immediate early response 3-interacting protein 1 OS=Rattus P85007 1.225574395 0.003189177 norvegicus GN=Ier3ip1 PE=3 SV=1 - [IR3IP_RAT] UDP-glucuronosyltransferase OS=Rattus norvegicus F1LTB8 1.22465606 0.003317991 GN=RGD1559459 PE=1 SV=2 - [F1LTB8_RAT] Carboxylesterase 1C OS=Rattus norvegicus GN=Ces1c PE=1 P10959 1.252466596 0.003546064 SV=3 - [EST1C_RAT] Cysteine-sulfinate decarboxylase OS=Rattus norvegicus B3VPA7 1.463542701 0.003795817 GN=Csad PE=2 SV=1 - [B3VPA7_RAT] Myotilin-like OS=Rattus norvegicus GN=LOC100909868 M0R5Y4 1.682931624 0.003932265 PE=4 SV=1 - [M0R5Y4_RAT] Vacuolar protein