Sample Preparation Product Catalog Chromatography Sample Dialysis Equilibrium Dialysis Electrodialysis Sample Filtration www.harvardapparatus.com Easy-to-Use Quick Start Guides Complete with assembly diagrams, step-by-step instructions, and related product ordering information. Hard copy included with every QuikPrep sample preparation product shipment. Available for electronic download from the Harvard Apparatus website. www.harvardapparatus.com/manualsUltra-Micro DispoDialyzer (1 to 5 µl Sample Volume) 96-Well ™ ™ SpinColumnsQuick Start Guide DispoEquilibrium(25 to 150 Dialyzer μl) (25 to 75 μl Sample Volume) ™ Macro Quick Start TheGuide Ultra-Micro DispoDialyzer is a disposable (single-use SpinColumnsl SampleQuick Volume) Start Guide only) dialyzer for the processing of extremely small samples ™μ from 1 µl to 5 µl. Dialysis is carried out inside a micro centrifuge tube. (75 to 150 Sample recovery is easy with almost 100% sample recovery. Membranes are preinstalled with molecular weight cut offs Description Instructions For Silica Columns: from 1,000 to 50,000 Daltons. The Ultra-Micro DispoDialyzer 96-Well SpinColumns bringcomes speed with and two simplicity1. 1.5 Place ml 25capped to µl to 75µlmicro of centrifuge buffer intoa) Pipette collection one chamber 200 μl of of organic the solvent into open wells. tubes—one for theDispoEquilibrium dialysis and one Dialyzer for collection using the special pipette tips Quick StartDescription Guide high-throughput micro-sample preparation. Both Micro b) Centrifuge for 2 minutes at provided. The DispoEquilibrium DialyzerSpinColumns allows for and equilibriumInstructions Macro SpinColumns dialysis are suitable for 2000 x g to equilibrate. Note: Hold DispoEquilibrium Dialyzer at slightly less than of samples from 25 µl to 75automation µl with a built-inand available1. regenerated Tap the with column our completegently to ensure range that of the media is settled DispoEquilibrium Dialyzer 45° angle when loading4. to Remove avoid trapping the SpinColumn air bubbles from at the collection cellulose or cellulose acetatepacking membrane. materials. Optimal Theyat the forcan bottom. binding be pre-packed with custom and interaction studies, the DispoEquilibrium Dialyzer is the membrane. plate and blot dry any moisture on the exterior (25 to 75 μl Sample Volume) requested materials.2. IntendedRemove the for red single caps use and only. place into a centrifuge tube. made of inert PTFE allowing for maximum sample retention. 2. Push the black cap into placeof the oncolumn. buffer side. Designed for one time use, the DispoEquilibrum3. Place Dialyzer 500 μlis of water or buffer (use 1000 μl for G-100 Gel Filtration) in the column3. Place and wait an equivalent15 minutes volume for5. Pipettehydration. of sample 50 to 100into μlopposite of sample to the top of the well, idealInstructions in studies which use radiolabeled compounds, saving Description Instructions chamber using a new pipetteplacing tip. the sample directly in the center of the wells. you thePlace time 25 of µl having to 75µl to of clean buffer the into chamber one chamber after4. Centrifuge ofeach the use. for 4 minutes at approximately 2000 x g. Macro SpinColumns provide1. rapid purification of samples Note: Hold DispoEquilibrium Dialyzer at slightly less than Quick Start Guide Each DispoEquilibriumDispoEquilibrium DialyzerDialyzer1. Tap using has the two theSpinColumn specialchambers,5. pipette Repeat gently one tips Steps to ensure 3 and that4 if desired. the dry 6. Place the column plate into a new collection using a single-use, disposable centrifuge tube format. 45° angle when loading to avoid trapping air bubbles at for bufferprovided. and one for the sample.column It comesmaterial with is 6.settled two Remove caps at thecolumn bottom. from tube and blot the exteriorplate dry. and spin for 2 minutes at 2000 x g. For Centrifugation or filtration under vacuum or pressure can be the membrane. used to run the sample(one through black,Note: theone Hold columns. white), DispoEquilibrium two Available 0.65 mlwith Dialyzermicrocentrifuge at slightly tubes less than some applications, such as gel filtration, the 2. Remove the foil fromInstructions7. asAdd many between rows 75 as μl desiredand 150 forμl of sample to the column. our complete range toof collectpacking45° and materials angle hold when samples, or pre-packed loading and to twoavoidwith pipette trapping tips air for bubbles sample at 4. Push the white cap intopurified place on sample sample is side.now ready for further use. your application using8. Placea razor the or column other sharpin a new blade. centrifuge tube and spin for custom requested materials.delivery andthe recovery.membrane. 1. Flip 4open minutes the coverat approximately of the sample 2000 tube x g. and remove 5. Gently agitate the DispoEquilibrium7. If step 5 results Dialyzer in binding on a shakerof the sample to the 2. Push the black cap3. Place into place the SpinColumn on bufferthe side. Dialyzer.into one of the until equilibrium is reached. Size Exclusion Applications: column bed, next elute the sample. Add a suitable collection tubes and follow instructions below. Description 3. Place an equivalent volume of sample2. Place into opposite your sample into theNote: Dialyzer. Lay the DispoEquilibrium Dialyzer flat to orient The purified sample is collected in the centrifugevolume tube. of the desired elution buffer to the wells chamber using a new pipette tip. membrane in vertical position and maintain fluid contact The DispoEquilibrium Dialyzer allows for equilibrium dialysis For Gel Filtration/3. Add Ion 500Exchange µl of buffer Columns: to the sample tube. and spin for 2 minutes at 2000 x g. If necessary, of samples from 25 µl to 75 µl with a built-in regenerated Note: Hold DispoEquilibrium Dialyzer at slightlySolid Phase less than Extraction with membrane Technique: (see drawing below). 45° angle whena) loading Pipette to 200avoid4. μl Placetrapping of buffer Dialyzer air intobubbles with all openatsample wells. into the sample tube.the columns can be washed with a suitable buffer to cellulose or cellulose acetate membrane. Optimal for binding Unbound sample componentsCAUTION: areViolent removed. agitation Place willcolumn result in air bubble the membrane. 5. Close the cover of the sample tube. remove contaminants before elution of the sample. and interaction studies, the DispoEquilibrium Dialyzer is b) Wait 15 minutes for hydration.into a new centrifugeentrapment tube, add elution at the buffermembrane, and centrifuge which may either made of inert PTFE allowing for maximum sample retention. 6. When dialysisto recover is complete, desiredincrease sample.remove the dialysis Dialyzer. timeIt is ornot prevent necessary equilibrium to use a fromfresh collection plate for Designed for one time use, the DispoEquilibrum Dialyzer is 4. Push the whitec) capCentrifuge into place plate on samplefor 2 minutes side. at 2000 x g. being attained. each washing step. ideal in studies which use radiolabeled compounds, saving 5. Gently agitate the DispoEquilibriumNotes: Dialysis Dialyzer times on a shaker vary from 2 hours to overnight. until equilibriumd) Wash is reached. column as needed for your application. you the time of having to clean the chamber after each use. Sample volume, buffer volume,6. Uncap chemistry the sample Notes:of samples chamber and spin out the sample using Note: Lay the DispoEquilibrium Dialyzer flat to orient the microcentrifuge tube provided. Each DispoEquilibrium Dialyzer has two chambers, one and MWCO of membranes can all affect timeSpinColumns required are intended for single use only. Quality of for buffer and one for the sample. It comes with two caps membrane in vertical positionfor complete and maintain dialysis. fluid Change contact buffer solution after Note: Spin at resultsapproximately cannot 500be guaranteed x g for a few if seconds.plate is re-used. (one black, one white), two 0.65 ml microcentrifuge tubes with membrane (see drawing2 hours below). and if needed after 4 hours. 7. Uncap the buffer chamber and spin out the buffer using to collect and hold samples, and two pipette tips for sample CAUTION: Violent7. agitationOnce dialysis will result is complete, in air bubble either extract your sample with the microcentrifuge tube provided. delivery and recovery. Macro Spincolumns™ are intendedentrapment for single at usethe aonly.membrane, pipette, or whichinvert themay dialyzer either into a new collection tube increaseOrdering dialysis Information andtime briefly or prevent centrifuge equilibrium (500 to from 2,000 rpm for 1 to 2 sec). being attained. 8. Centrifuge briefly (500 to 2,000 rpm for 1 to 2 sec) 6. UncapEmpty the sample SpinColumns chamber and spin out the sample using the microcentrifugeto transfer tube provided.the remainder of the sample into the Frit collection tube. 96-well Micro SpinColumns 96-well Macro SpinColumns Note: Spin at approximately 500 x g for a few seconds. 7 µm frit 74-5635 74-5649 7. Uncap the buffer chamber and spin out the buffer using 25the µm microcentrifuge frit tube provided.74-5610 74-5650 9511-032 Rev 2.1 QuikPrep • email: [email protected] • harvardapparatus.com QuikPrep • email: [email protected] • harvardapparatus.com 9511-033 Rev 2.0 9511-043 Rev 2.1 9511-026 Rev 2.0 • harvardapparatus.com 9511-026 Rev 2.0 • email: [email protected] QuikPrep QuikPrep • email: [email protected] • harvardapparatus.com • harvardapparatus.com • email: [email protected] QuikPrep INTRODUCTION QuikPrep® Sample Preparation Products Small Volume