Histology BY Dr Navneet Kumar Professor Anatomy KGMU LKO -Bone Histological features Types of bone -Cartilage Histological features Types of bone Dr. Navnne Kumar Professor Anatomy K G M U Lko Bone Histology • Bone membrane- Periosteum Endosteum • 1.Cells- Osteoblast - Osteocyte - Osteoclast • 2.Fibres(95%)- Osteoclast -collagen fibre Type1 • 3.Ground substance(5%) -Calcium phosphate 85% -calcium carbonate 10% Dr. Navnne Kumar Professor Osteocyte Anatomy K G M U Lko Dr. Navnne Kumar Professor Anatomy K G M U Lko Dr. Navnne Kumar Professor Anatomy K G M U Lko Types of Bone • .Lamellar, Mature bone- 1. Compact bone - circumferential lamellae outer (periosteal) Inner (endosteal) - interstitial lamellae - haversion system (osteon) 2. Spongy, Cancellous, Trabecular bone - interstitial lamellae • .Woven, Immature,Primary bone- - no lamellae e.g –fetal bone ,callus Dr. Navnne Kumar Professor Anatomy K G M U Lko Compact bone • Lacuna have Osteocytes • Canaliculi contains the cytoplasmic process of Osteocytes • Haversian canal contains a blood vessel • Circular arrangement of osteocytes and fibres aruond a blood vessel is an osteon(Havesian canal system) • Volkmann canal- interconnecting haversian canal • Bone marrow-central core cavity of bone Dr. Navnne Kumar Professor Anatomy K G M U Lko Dr. Navnne Kumar Professor Anatomy K G M U Lko Dr. Navnne Kumar Professor Anatomy K G M U Lko Compact bone Dr. Navnne Kumar Professor Anatomy K G M U Lko Dr. Navnne Kumar Professor Anatomy K G M U Lko Dr. Navnne Kumar Professor Anatomy K G M U Lko Dr. Navnne Kumar Professor Anatomy K G M U Lko Spongy Bone • Branched bony trabeculae • Encloses bone marrow cavity • Cells and matrix interstitial lamellae type Dr. Navnne Kumar Professor Anatomy K G M U Lko Dr. Navnne Kumar Professor Anatomy K G M U Lko Dr. Navnne Kumar Professor Anatomy K G M U Lko Woven Bone • Random , interlacing collagen fibres • Immature cells are present Dr. Navnne Kumar Professor Anatomy K G M U Lko Cartilage • Perichondrium - may be present or absent • Cells - Chondrocytes • Matrix –Fibres • . Collagen fibres • .Elastic fibres • - Ground substance • .Chondrotin sulphates • . Keratan sulphates • . Aggrecan,water electrolyte • Types- Hyaline cartilage • -Elasticcartilage (yellow fibrocartilage) • -FibrocartilageDr. Navnne (Kumarwhite Professor fibrocartilage ) Anatomy K G M U Lko Hyaline Cartilage • Perichondriun present • Chondrocytes in lacunae as cell nests (isogenous cell group) • Matrix is homogenous contains collagen fibres e.g-Costal,Articular Tracheal, Thyroid, Epiphysis Dr. Navnne Kumar Professor Anatomy K G M U Lko Dr. Navnne Kumar Professor Anatomy K G M U Lko Dr. Navnne Kumar Professor Anatomy K G M U Lko Dr. Navnne Kumar Professor Anatomy K G M U Lko Elastic cartilage • Perichondriun present • Matrix filled with elastic fibres • Elastic fibres are branched. more at surface • Chondrocytes in lacunae • eg –auricle, epiglottis Dr. Navnne Kumar Professor Anatomy K G M U Lko Dr. Navnne Kumar Professor Anatomy K G M U Lko Dr. Navnne Kumar Professor Anatomy K G M U Lko Fibro cartilage • Perichondriun absent • Bundle of collagen fibres • Chondrocytes present in rows • e.g-intervertivral disc ,glenoidal labrum Dr. Navnne Kumar Professor Anatomy K G M U Lko Dr. Navnne Kumar Professor Anatomy K G M U Lko .