LEGIONELLA 2009 October 13-17, 2009 Institut Pasteur, Paris, France SCIENTIFIC COMMITTEE Paola BORELLA Elizabeth HARTLAND University of Modena and Reggio Emilia, University of Melbourne, Melbourne, Australia Modena, Italy Carol JOSEPH Carmen BUCHRIESER Health Protection Agency, London, United Institut Pasteur, Paris, France Kingdom Nicholas P. CIANCIOTTO Jaana KUSNETSOV Northwestern University Medical School, National Public Health Institute, Kuopio, Finland Chicago, USA Christian LÜCK Didier CHE National Reference Center for Legionella, Institut de Veille Sanitaire, Saint-Maurice, Dresden, Germany France Carmen PELAZ Paul H. EDELSTEIN Instituto de Salud Carlos III, Madrid, Spain University of Pennsylvania Medical Center, Philadelphia, USA Rod RATCLIFF Institute of Medical & Veterinary Science, Jérôme ETIENNE Adelaide, Australia National Reference Center for Legionella, Lyon, France Michele S. SWANSON University of Michigan Medical Center, Ann Barry FIELDS Arbor, USA Center of Disease Control (CDC), Atlanta, USA Keizo YAMAGUCHI Jörg HACKER Toho University, Tokyo, Japan Robert Koch Institut, Berlin, Germany ORGANIZING COMMITTEE Carmen BUCHRIESER Institut Pasteur, Paris, France Jérôme ETIENNE National Reference Center for Legionella, Lyon, France Didier CHE Institut de Veille Sanitaire, Saint-Maurice, France ENTIFIC ACKNOWLEDGEMENTS We are grateful to the following organisations for their generous financial support Nous remercions chaleureusement nos partenaires financiers INSTITUT PASTEUR EUROPEAN MOLECULAR BIOLOGY ORGANIZATION (EMBO) FEDERATION OF EUROPEAN MICROBIOLOGICAL SOCIETIES (FEMS) NETWORK OF EXCELLENCE “EUROPATHOGENOMICS” (NOE EPG) OF THE EUROPEAN COMMISSION REGION ILE DE FRANCE INSTITUT NATIONAL DE VEILLE SANITAIRE ACKNOWLEDGEMENTS We are grateful to our sponsors Nous remercions chaleureusement nos sponsors SPONSORS SPONSORS OTHER PARTNERS TABLE OF CONTENTS Theme of the conference..............................................................................................I Scientific program........................................................................................................II Oral communications...................................................................................................1 Opening session.......................................................................................................................................2 Session 1: Epidemiology and Clinical aspects: a) disease, diagnosis and treatment .............................6 Session 2: Clinical aspects: b) detection and subtyping ........................................................................12 Session 3: Host - Microbe interactions: a) secretion systems and their substrates...............................18 Session 4: Host - Microbe interactions: b) metabolism and virulence ...................................................24 Session 5: Immunology and host susceptibility......................................................................................28 Session 6: Microbe - Environment interactions: a) cell biology, protozoa, biofilm.................................33 Session 7: Microbe - Environment interactions: b) detection in natural and artificial reservoirs - survival, persistence ...... 39 Session 8: Legionella prevention and control ........................................................................................44 Session 9: Epidemiology: a) nosocomial infections and Legionella control in hospitals .......................48 Session 10: Epidemiology: b) outbreaks and population genetics and taxonom...................................54 Session 11: Genomics and Comparative genomics ..............................................................................60 Session 12: Functional genomics ..........................................................................................................65 Session 13: Gene regulation in the environment and the host ..............................................................70 Session 14: Cell biology - protozoa – macrophages..............................................................................74 Posters ......................................................................................................................78 Session 1: Epidemiology and Clinical aspects: a) disease, diagnosis and treatment ...........................79 Session 2: Clinical aspects: b) detection and subtyping ..................................................................... 107 Session 3: Host - Microbe interactions: a) secretion systems and their substrates............................ 126 Session 4: Host - Microbe interactions: b) metabolism and virulence ................................................ 141 Session 5: Immunology and host susceptibility...................................................................................158 Session 6: Microbe - Environment interactions: a) cell biology, protozoa, biofilm.............................. 165 Session 7: Microbe - Environment interactions: b) detection in natural and artificial reservoirs - survival, persistence 179 Session 8: Legionella prevention and control ..................................................................................... 211 Session 9: Epidemiology: a) nosocomial infections and Legionella control in hospitals .................... 267 Session 10: Epidemiology: b) outbreaks and population genetics and taxonom................................ 277 Session 11: Genomics and Comparative genomics ........................................................................... 289 Session 12: Functional genomics ....................................................................................................... 297 Session 13: Gene regulation in the environment and the host ........................................................... 299 Authors index...........................................................................................................306 Detailed summary....................................................................................................315 Company profiles of the sponsors ...........................................................................337 THEME OF THE CONFERENCE The International Conference on Legionella is a premier international meeting held every four years to discuss new findings on Legionella pneumophila, other Legionella, and the disease caused by these organisms and also to identify knowledge gaps that exist in controlling Legionella infections of humans. These four-day meetings have been held in various locations in Europe and the USA. The 6th International Conference, Legionella 2005 in Chicago, was attended by many international leaders in Legionella research and attracted 400 attendees to participate in nearly 40 invited lectures, 200 poster presentations, three panel discussions, and numerous opportunities that fostered scientific collaboration. The 7th International Conference in this series, Legionella 2009, will address a wide range of current research and trends related to Legionella. Sessions will be dedicated to epidemiology and clinical aspects; pathogenesis and immunology; genetics and genomics; ecology and evolution; physiology, regulation, and biochemistry. The Conference will be of interest to researchers; those with clinical, public health and epidemiological interests; regulatory agency representatives; representatives from industry with interests in Legionella and Legionnaires’ disease. Participation by students and scientists in the early stages of their careers is highly encouraged. Legionella 2009 will provide abundant opportunities for networking and exchange of information between senior scientists and professionals, new investigators and students. La conférence internationale sur Legionella (LEGIONELLA 2009) est une rencontre internationale qui se tient tous les quatre ans pour discuter des nouvelles découvertes sur Legionella pneumophila et autres Légionelles, de la maladie causée par ces organismes ainsi que pour identifier des problèmes existant dans le contrôle des infections humaines à Legionella. Ces rencontres de quatre jours ont été organisées dans différentes villes européennes et aux Etats-unis. La 6ème conférence internationale, Legionella 2005 à Chicago, a vu la participation de nombreux leaders internationaux de la recherche sur Legionella et a attiré 400 personnes qui ont participé aux 40 séminaires invités, 200 présentations de posters, trois discussions générales, profitant également des nombreuses opportunités pour initier ou approfondir des collaborations scientifiques. La 7ème Conférence Internationale dans cette série, Legionella 2009, adressera de nombreuses questions de la recherche courante et de tendances concernant Legionella. Les thèmes abordés porteront sur l’épidémiologie et l es aspects cliniques, la pathogénicité et l’immunologie, la génétique et la génomique, l’écologie et l’évolution, la physiologie, la régulation et la biochimie. La conférence sera d’un grand intérêt pour les chercheurs, qu’ils soient intéressés par des questions cliniques, de santé publique ou par l’épidémiologie mais aussi pour les représentants des agences de contrôle, de gestion du risque et de surveillance ; les représentants de l’industrie intéressés par Legionella et la maladie de Légionnaire. La participation des étudiants et des chercheurs en début de carrière est fortement encouragée. Legionella 2009 fournira beaucoup d’opportunités pour la création de réseaux de travail, l’échange d’informations