BCL7A Antibody Cat. No.: 45-325 BCL7A Antibody 45-325 Immunofluorescence analysis of 45-325 Flow cytometric analysis of paraformaldehyde paraformaldehyde fixed U2OS cells, permeabilized with fixed Daudi cells (blue line), permeabilized with 0.5% 0.15% Triton. Primary incubation 1hr (10ug/ml) followed Triton. Primary incubation 1hr (10ug/ml) followed by Alexa by Alexa Fluor 488 secondary antibody (2ug/ml), showing Fluor 488 secondary antibody (1ug/ml). IgG control: nuclear staining. Actin filaments were stained wit Unimmunized goat IgG (black line) fo Specifications HOST SPECIES: Goat SPECIES REACTIVITY: Human HOMOLOGY: Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow, Pig IMMUNOGEN: The immunogen for this antibody is: C-MKLEASQQNSEEM TESTED APPLICATIONS: ELISA, Flow, IF, WB October 2, 2021 1 https://www.prosci-inc.com/bcl7a-antibody-45-325.html Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 26kDa band observed in Human Lymph Node lysates (calculated MW of 25.0kDa according to NP_066273.1). Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at APPLICATIONS: room temperature.Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of Daudi cells. Recommended concentration: 10ug/ml. This antibody is expected to recognize both reported isoforms (NP_066273.1; SPECIFICITY: NP_001019979.1). POSITIVE CONTROL: 1) Cat. No. 1201 - HeLa Cell Lysate Properties Purified from goat serum by ammonium sulphate precipitation followed by antigen PURIFICATION: affinity chromatography using the immunizing peptide. CLONALITY: Polyclonal CONJUGATE: Unconjugated PHYSICAL STATE: Liquid Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum BUFFER: albumin. Aliquot and store at -20°C. Minimize freezing and thawing. CONCENTRATION: 500 ug/mL STORAGE CONDITIONS: Aliquot and store at -20˚C. Minimize freezing and thawing. Additional Info OFFICIAL SYMBOL: BCL7A ALTERNATE NAMES: BCL7A, BCL7, B-cell CLL/lymphoma 7A, B-cell CLL/lymphoma-7 ACCESSION NO.: NP_066273.1, NP_001019979.1 PROTEIN GI NO.: 10337613 GENE ID: 605 Background and References 1) Zani VJ, Asou N, Jadayel D, Heward JM, Shipley J, Nacheva E, Takasuki K, Catovsky D, Dyer MJ. Molecular cloning of complex chromosomal translocation REFERENCES: t(8;14;12)(q24.1;q32.3;q24.1) in a Burkitt lymphoma cell line defines a new gene (BCL7A) with homology to caldesmon. Blood 1996 Apr 15;87(8):3124-34 ANTIBODIES FOR RESEARCH USE ONLY. October 2, 2021 2 https://www.prosci-inc.com/bcl7a-antibody-45-325.html For additional information, visit ProSci's Terms & Conditions Page. October 2, 2021 3 https://www.prosci-inc.com/bcl7a-antibody-45-325.html.