Product Datasheet Cadherin-16 Antibody (CDH16/1071) NBP2-45157-0.1mg Unit Size: 0.1 mg Store at 4C. Protocols, Publications, Related Products, Reviews, Research Tools and Images at: www.novusbio.com/NBP2-45157 Updated 5/19/2021 v.20.1 Earn rewards for product reviews and publications. Submit a publication at www.novusbio.com/publications Submit a review at www.novusbio.com/reviews/destination/NBP2-45157 Page 1 of 2 v.20.1 Updated 5/19/2021 NBP2-45157-0.1mg Cadherin-16 Antibody (CDH16/1071) Product Information Unit Size 0.1 mg Concentration 0.2 mg/ml Storage Store at 4C. Clonality Monoclonal Clone CDH16/1071 Preservative 0.05% Sodium Azide Isotype IgG1 Kappa Purity Protein A or G purified Buffer 10 mM PBS with 0.05% BSA Target Molecular Weight 130 kDa Product Description Host Mouse Gene ID 1014 Gene Symbol CDH16 Species Human, Mouse, Rat, Canine, Rabbit Specificity/Sensitivity This MAb recognizes a protein of 130kDa, identified as Ksp-cadherin. Cadherins form a superfamily of related glycoproteins that mediate calcium-dependent cell adhesion and transmit signals from the extracellular matrix to the cytoplasm. Cadherins have been implicated in embryogenesis, tissue morphogenesis, tissue structure maintenance, cell polarization, neoplastic invasiveness and metastasis, and membrane transport. It is suggested that Ksp-cadherin is a marker for terminal differentiation of the basolateral membranes of renal tubular epithelial cells. Within the kidney, Ksp-Cadherin is found exclusively in the basolateral membrane of renal tubular epithelial cells and collecting duct cells, and not in glomeruli, renal interstitial cells, or blood vessels.Ksp-Cadherin has been suggested to distinguish Chromophobe Renal-Cell Carcinoma from Oncocytoma. Immunogen Recombinant full-length human Cadherin-16 protein Product Application Details Applications Western Blot, ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin Recommended Dilutions Western Blot, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, ELISA 2-4 ug/ml, Immunohistochemistry, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml Application Notes ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA. Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. Novus Biologicals USA Bio-Techne Canada 10730 E. Briarwood Avenue 21 Canmotor Ave Centennial, CO 80112 Toronto, ON M8Z 4E6 USA Canada Phone: 303.730.1950 Phone: 905.827.6400 Toll Free: 1.888.506.6887 Toll Free: 855.668.8722 Fax: 303.730.1966 Fax: 905.827.6402 [email protected] [email protected] Bio-Techne Ltd General Contact Information 19 Barton Lane www.novusbio.com Abingdon Science Park Technical Support: [email protected] Abingdon, OX14 3NB, United Kingdom Orders: [email protected] Phone: (44) (0) 1235 529449 General: [email protected] Free Phone: 0800 37 34 15 Fax: (44) (0) 1235 533420 [email protected] Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt. For more information on our 100% guarantee, please visit www.novusbio.com/guarantee Earn gift cards/discounts by submitting a review: www.novusbio.com/reviews/submit/NBP2-45157 Earn gift cards/discounts by submitting a publication using this product: www.novusbio.com/publications.