Serbian Biochemical Society Seventh Conference "Biochemistry of Control in Life and Technology" Faculty of Chemistry Proceedings Belgrade 2017 Serbian Biochemical Society President: Marija Gavrović-Jankulović Vice-president: Suzana Jovanović-Šanta General Secretary: Milan Nikolić Treasurer: Milica Popović Scientific Board Milica Bajčetić David R. Jones Edvard T. Petri Duško Blagojević Suzana Jovanović-Šanta Natalija Polović Polina Blagojević Ivanka Karadžić Tamara Popović Jelena Bogdanović Vesna Kojić Željko Popović Pristov Jelena Kotur- Radivoje Prodanović Nataša Božić Stevuljević Niko Radulović Ivona Baričević-Jones Snežana Marković Ivan Spasojević Jelena Bašić Sanja Mijatović Karmen Stankov Tanja Ćirković Djordje Miljković Aleksandra Stanković Veličković Marina Mitrović Tijana Stanković Milena Ćurčić Jelena Nestorov Ivana Stojanović (ib) Milena Čavić Ivana Nikolić Ivana Stojanović (ibiss) Milena Despotović Milan Nikolić Aleksandra Uskoković Snežana Dragović Miroslav Nikolić Perica J. Vasiljević Marija Gavrović- Zorana Oreščanin- Milan Zarić Jankulović Dušić Aleksandra Zeljković Nevena Grdović Svetlana Paškaš Marko N. Živanović Lidija Israel-Živković Anđelka Petri Milan Žižić Proceedings Editor: Ivan Spasojević Technical secretary: Jelena Nestorov Cover design: Zoran Beloševac Publisher: Faculty of Chemistry, Serbian Biochemical Society Printed by: Colorgrafx, Belgrade Serbian Biochemical Society Seventh Conference with international participation Faculty of Chemistry, University of Belgrade 10.11.2017. Belgrade, Serbia “Biochemistry of Control in Life and Technology” PROGRAMME 10:00-10:10 Welcome message Marija Gavrović-Jankulović (President of the Serbian Biochemical Society) 10:10-10:40 Ario de Marco University of Nova Gorica, Vipava, Slovenia Nanobodies: Towards rational design of immune-reagents (FEBS3+ Lecture) 10:40-11:00 Vladimir P. Beškoski Faculty of Chemistry, University of Belgrade Classical biochemistry / biotechnology and molecular biochemistry / biotechnology of environmental microorganisms in degradation of petroleum products and persistent organic pollutants 11:00-11:20 Jakub Nowak NanoTemper Technologies, Krakow, Poland Microscale thermophoresis and NanoDSF as advanced methods in life science research 11:20-11:50 Coffee break 11:50-12:10 Marija Vidović Institute for Multidisciplinary Research, University of Belgrade Sun as a stressor and/or regulator of plant metabolism: responses to UV radiation and high light 12:10-12:30 Vladimir B. Mihailović Faculty of Science, University of Kragujevac Phytochemical characterization and biological activities of some Gentiana plants from Serbia 12:30-12:50 Marija Genčić Faculty of Science and Mathematics, University of Niš Phytochemical re-examination of well-studied medicinal plants as an useful approach in the discovery of (novel) potentially bioactive natural products – The case of Inula helenium L. 12:50-13:10 Dalibor Stanković The Vinča Institute of Nuclear Sciences, University of Belgrade Nano-structured materials and their application in the detection of biological compounds 13.10-13.40 Poster session 13.40-14.00 Cocktail 14:00-14:20 Ivana Beara Faculty of Sciences, University of Novi Sad Battle against inflammation: polyphenols targeting selective inhibition of cyclooxygenase-2 14:20-14:40 Tamara Saksida Institute for Biological Research “Siniša Stanković”, University of Belgrade The role of macrophage migration inhibitory factor in the development of obesity and altered intestinal permeability 14:40-15:00 Žanka Bojić Trbojević Institute for the Application of Nuclear Energy - INEP, University of Belgrade Galectin-1 ligands in human trophoblasts 15.00-15:20 Discussion and concluding remarks Poster Session Ana Marija Balaž Center of Chemistry, Institute of Chemistry, Technology and Metallurgy, University of Belgrade Semi-rational design of cellobiose dehydrogenase from Phanerochaete chrysosporium for increased oxidative stability and high-throughput screening of library mutants Sanja Berežni Department of Chemistry, Biochemistry and Environmental Protection, Faculty of Sciences, University of Novi Sad Novel lignans from Anthriscus sylvestris: 3'-demethoxy podophyllotoxin and 3'- demethoxypodophyllotoxone Stefan Z. Blagojević Department of Biology and Ecology, Faculty of Science, University of Kragujevac Statistical clustering of IC50 values as bioactive substances cytotoxicity indicators on HCT-116 and SW-480 cell lines of colon cancer Petar Čanović Department of Biochemistry, Faculty of Medical Sciences, University of Kragujevac Two newly syhtesized ruthenium(II) polypyridyl complexes induce selective apoptosis of HeLa cancer cells via mitochondrial pathway Nikola Gligorijević Institute for the Application of Nuclear Energy - INEP, University of Belgrade Influence of fibrinogen modifications on its interaction with insulin-like growth factor-binding protein 1 Tamara Janković Department of Medical Biochemistry, Faculty of Pharmacy, University of Belgrade In vitro comparison of antioxidative potential of differently substituted chalcones Jaroslav Katrlík Institute of Chemistry, Slovak Academy of Sciences Determination of protein glycosylation using lectin-based protein microarrays Jelena Korać Department of Life Sciences, Institute for Multidisciplinary Research, University of Belgrade Ligand and redox interactions of adrenaline with iron at physiological pH Gordana Kovačević Innovation Center of the Faculty of Chemistry, University of Belgrade Protein engineering and development of high-throughput screening methods for glucose-oxidase gene library Branka Lončarević Department of Chemistry, Institute of Chemistry, Technology and Metallurgy, University of Belgrade Application of microbial levan as a new component for production of graft copolymer with polystyrene Ivana Lukić Department of Research and Development, Institute of Virology, Vaccine and Sera „Torlak“, Belgrade The role of heterologous immunity in resistance to ocular chlamydial infection Tatjana Majkić Department of Chemistry, Biochemistry and Environmental Protection, Faculty of Sciences, University of Novi Sad Antioxidant and anti-inflammatory activity of Merlot wine Emilija Marinković Institute of Virology, Vaccines and Sera - Torlak, Belgrade The effect of prophylactic treatment of recombinant banana on TNBS-induced colitis in BALB/C mice Nevena Marković Institute of Chemistry, Technology and Metallurgy, University of Belgrade Synthesis of potential pharmaceutical active ingredients using omega-transaminase Ana Medić School of Medicine, University of Belgrade Biodegradation of 2,6-di-tert-butylphenol by Pseudomonas aeruginosa san ai Ivana Milenković Department of Life Science, Institute for Multidisciplinary Research, University of Belgrade The influence of coated nanoCeO2 on the phenol content in wheat and pea Ivan Mrkić Innovation Centre of Faculty of Chemistry, University of Belgrade Newly designed soluble hemagglutinin-Der p 2 chimera is a potential candidate for allergen specific immunotherapy Ivana Nemeš Department of Chemistry, Biochemistry and Environmental Protection, Faculty of Sciences, University of Novi Sad Chemical composition and antioxidant potential of hydromethanolic extracts of celery (Apium graveolens L.) cultivated at different locations in Vojvodina Danijela Nikodijević Department for Biology and Ecology, Faculty of Science, University of Kragujevac Effects of the bee products on energy status and relative expression of biotransformation and apoptosis genes in healthy and colon cancer cells Teodora Obradović Department of Medical Biochemistry, Faculty of Pharmacy, University of Belgrade Comparison of prooxidative properties of four different tyrosine kinase inhibitors Miloš Opačić Department of Life Sciences, Institute for Multidisciplinary Research, University of Belgrade Imaging and regional distribution of copper, zinc, manganese and iron in sclerotic hippocampi of patients with mesial temporal lobe epilepsy Snežana Orčić Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad Environmental effect on metallothionein gene expression in honey bee (A. mellifera) Marijana Pavlović Department of Experimental Oncology, Institute for Oncology and Radiology of Serbia, Belgrade Mechanism of action of novel ruthenium(III) complexes toward cisplatin resistant MDA-MB231 breast cells Vladana Petković Vinča Institute of Nuclear Sciences, University of Belgrade Direct effects of γ-rays in MCF-7 breast cancer cells Diandra Pintać Department of Chemistry, Biochemistry and Environmental Protection, Faculty of Sciences, University of Novi Sad Is wine price a guarantee for its healthful properties? Jovana J. Plavša Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad Identification of potential inhibitors of AKR1C3 and preparation for structural analyses Ivana Prodić Faculty of Chemistry – Innovation Centre Ltd., University of Belgrade Whole grain of peanut digestomics according to harmonized static digestion protocol suitable for solid food and characterization of short digestion resistant fragments. Marija Stanojević Institute for Pathological Physiology, Faculty of Medicine, University of Belgrade Minor depolarization mediates magnesium suppression of nonsynaptic epileptiform activity Nikola Tatalović Department of Physiology, Institute for Biological Research “Siniša Stanković”, University of Belgrade The effects of ibogaine on uterine redox homeostasis and contractility Tamara Uzelac Faculty of Chemistry, University of Belgrade Serum