Authors: Pallavi Subhraveti Ron Caspi Quang Ong Peter D Karp An online version of this diagram is available at BioCyc.org. Biosynthetic pathways are positioned in the left of the cytoplasm, degradative pathways on the right, and reactions not assigned to any pathway are in the far right of the cytoplasm. Transporters and membrane proteins are shown on the membrane. Ingrid Keseler Periplasmic (where appropriate) and extracellular reactions and proteins may also be shown. Pathways are colored according to their cellular function. Gcf_002025645Cyc: Curtobacterium pusillum AA3 Cellular Overview Connections between pathways are omitted for legibility. Anamika Kothari D-mannitol phosphate phosphate phosphate putrescine glycine betaine glycine betaine ammonium ammonium Fe 2+ D-gluconate ammonium H + spermidine gly gly enterobactin predicted predicted PEP-driven ABC RS16670 RS10785 RS04295 RS03485 RS01160 RS13465 RS13470 AtpD RS14785 RS17505 RS12115 transporter transporter of D-mannitol of phosphate putrescine glycine betaine glycine betaine ammonium ammonium phosphate phosphate Fe 2+ D-gluconate ammonium H + spermidine gly gly enterobactin D-mannitol 1-phosphate phosphate Amino Acid Degradation Cofactor, Prosthetic Group, Electron Carrier, and Vitamin Biosynthesis Degradation/ Macromolecule Modification lipoprotein posttranslational Utilization/ ditrans,octacis- 1-stearoyl- DNA combined 3-oxo-2-(cis- a (7Z,10Z,13Z a [protein]-L- modification L-valine degradation I Assimilation - Other a 3-oxoglutaryl- (S)-1-pyrroline- [2Fe-2S] iron-sulfur cluster biosynthesis L-glutamine O-acetyl-L-serine hydrogen selenide a palmitoyl-[acp] sn-glycerol with exogenous 2'-pentenyl)- a protein H O diphosphate ,16Z,19Z)-3- met menaquinol-9 biosynthesis 1,4-dihydroxy-2- Aminoacyl-tRNA Charging L-methionine degradation L-serine degradation L-arginine degradation X (arginine a tRNA precursor CTP ATP undecaprenyldiphospho- acetyl-CoA 2 dITP ITP [acp] methyl ester 5-carboxylate methionine superpathway of tetrahydrofolate biosynthesis demethylmenaquinol- molybdenum cofactor biosynthesis di-trans,poly-cis-undecaprenyl lipoate biosynthesis a [prolipoprotein]- acrylonitrile peptide- I (to L-homocysteine) degradation I bifunctional o- N-acetyl-(N- 1-acyl-sn-glycerol-3-phosphate DNA to form a cyclopentane-1- ATP-dependent inorganic 3-oxoacyl-ACP oxodocosa- 1-pyrroline-5- bifunctional 9 biosynthesis naphthoate biosynthesis phosphate biosynthesis and incorporation I monooxygenase pathway) acetyl-CoA C- non-canonical purine non-canonical purine L-cysteine degradation I CCA tRNA recombinational hexanoyl-CoA pyrophosphatase: 7,10,13,16,19- carboxylate methionine tRNA charging val acetylhomoserine/o- acetylglucosaminyl) 3-phosphate crossover junction Clp protease reductase FabG: 3-oxoacyl-ACP glutamate N- predicted L-cysteine desulfurase all-trans- demethylmenaquinol-9 nucleotidyltransferase: acyltransferase: NTP pyrophosphatase, NTP pyrophosphatase, (S)-S-oxide chorismate cys (2E,6E)-farnesyl demethylmenaquinol- L-asparagine prolipoprotein ser acetylserine sulfhydrylase: muramoyl-L-alanyl- acyltransferase: junction proteolytic subunit: BUE88_RS12210 BUE88_RS02435 pentaenoyl-[acp] dehydrogenase: acetyltransferase/ ABC GTP 2-carboxy-1,4- demethylmenaquinone met arg gln acrylonitrile endodeoxyribonuclease BUE88_RS08345 RdgB/HAM1 family: rdgB RdgB/HAM1 family: rdgB reductase FabG: nonaprenyl an octanoyl-[acp] biosynthesis III diacylglyceryl BUE88_RS11360 γ BUE88_RS12075 reductase: cysteine naphthoquinol isochorismate ATP diphosphate 8 biosynthesis I branched-chain methionine L-serine CTP synthase: BUE88_RS03525 -D-isoglutaminyl- BUE88_RS04005 RuvC: BUE88_RS01990 BUE88_RS17195 inorganic 3-oxoacyl-ACP BUE88_RS02435 amino-acid met transporter met diphosphate methyltransferase: lipoate-protein arginine-- acetyl-CoA C- NTP NTP msrA 1,4-dihydroxy- synthase: cysteine arg (tRNA-dependent) transferase: ammonia-lyase: BUE88_RS08675 CCA tRNA N-(β-D-asparaginyl)peptidoglycan pyrophosphatase: 1-pyrroline-5- acetyltransferase of L- desulfurase: BUE88_RS05490 tRNA ligase: amino acid adenosyltransferase: O-acetylhomoserine [+ 2 isozymes]1-stearoyl- Holliday junction ATP-dependent Clp reductase: 3-oxoacyl-ACP GTP 2-naphthoate ligase B: 2-carboxy-1,4- BUE88_RS02710 nucleotidyltransferase: di-trans,octa-cis a peptidoglycan acyltransferase: pyrophosphohydrolase: pyrophosphohydrolase: BUE88_RS05875 BUE88_RS05495 desulfurase: GTP di-trans,octa-cis aminotransferase: BUE88_RS02805 2-aminoprop-BUE88_RS10735 [+ 5 isozymes] aminocarboxypropyltransferase/ -L-lysyl-D-alanineglycosyltransferase: protease proteolytic BUE88_RS15140 BUE88_RS14775 carboxylate ArgJ: peptide- methionine cyclohydrolase I: menaquinol-9 a [lipoyl-carrierBUE88_RS03925 naphthoquinol BUE88_RS03190 an L-arginyl- asp 4-guanidinobutyramide 2-palmitoyl- resolution ofresolvase RuvX: 3-oxo-2-(cis-BUE88_RS01125 BUE88_RS11945 BUE88_RS11945 a (3R)-3- a (3R,7Z,10reductase:Z polyprenyltransferase: isochorismate a [prolipoprotein]- acrylamide a tRNA BUE88_RS19420 -undecaprenyl dimer acyl-carrier protein + BUE88_RS05875 -undecaprenyl 6 1,4-dihydroxy- BUE88_RS04210 SAM 2-enoate Glu cysteine synthase: BUE88_RS18770 subunit: clpP phosphate H dehydrogenase: BUE88_RS08755 methionine cysteine BUE88_RS10485 protein] N - [tRNA Arg ] aspartate-- sn-glycerol recombinationalBUE88_RS02905 2'-pentenyl)- hydroxyglutaryl- ,13Z,16Z,19ZBUE88_RS14775)-3- met RS11125 met BUE88_RS05515 diphosphate 2-naphthoate (S-diacylglyceryl) containing a diphosphate (tetrapeptide) dIMP IMP BUE88_RS18480 (S)-S-oxide cysteine Arg 3-methyl-2- BUE88_RS03670 cyclopentane-1- desulfurase: 7,8- demethylmenaquinol-9 2-succinyl-5- GTP 3',8- octanoyl-L-lysine a tRNA tRNA(Asn) ligase: -L-cysteine amidase: amidase: 3-phosphate junction a peptide a peptide [acp] methyl ester hydroxydocosa- N-acetylglutamate desulfurase: a carboxy- undecaprenyl- polyprenyltransferase: 3' CCA end reductase: BUE88_RS03730 dihydroneopterin cyclase MoaA: lipoyl BUE88_RS05585 oxobutanoate BUE88_RS03345 BUE88_RS03345 acetate L-selenocysteine formation of two (3-oxooctanoyl) 7,10,13,16,19- Glu synthase: enolpyruvyl- adenylated diphosphate BUE88_RS05515 an L-aspartyl- msrA 3'-triphosphate BUE88_RS03730 BUE88_RS07215 synthase: intact strands -CoA pentaenoyl-[acp] BUE88_RS12310 6-hydroxy-3- Asn signal a protein-L- an S-sulfanyl-[L- an [L-cysteine [small subunit of (8S)-3',8-cyclo-7,8- phosphatase: demethylmenaquinol-8 [tRNA ] N-acetyl-L- asn RS04310 asn lipA peptidase 2-iminopropanoate 4-guanidinobutanoate acrylate methionine- cysteine desulfurase] cyclohexene- desulfurase]-S- molybdopterin BUE88_RS02280 a [lipoyl-carrier dihydroguanosine Asp-tRNA(Asn) SAH methionine 1-carboxylic- di-trans,octa-cis 6 II: lspA (S)-S-oxide sulfanyl-L-cysteine synthase] 5'-triphosphate protein]-N - cysteine-- -undecaprenyl cys /Glu-tRNA(Gln) isobutanoyl-CoA 7,8- acid synthase: lipoyl-L-lysine tRNA ligase: signal cyclic pyranopterin amidotransferase adenosylhomocysteinase: L-threonine dihydroneopterin menD phosphate BUE88_RS09305 peptidase predicted monophosphate an L-cysteinyl- BUE88_RS04500 degradation II 1-stearoyl- a peptide with 3-oxo-2-(cis- (5E)-3-oxo- a (9Z)-3-oxo- a (5Z)-3- (R)-4-hydroxy- an S-sulfanyl-[cysteine subunit GatC: O-acetyl-L- a 2,3,4-saturated a 2,3,4-saturated 2- (+)-7-epi-jasmonoyl-CoA acetyl-CoA an unsulfurated a 3-oxoicosanoyl- a phosphate ABC efflux 3'-phosphate 2-succinyl-5- [tRNA Cys ] II: lspA 4-aminobutanoate methanethiol a stearoyl-[acp] sn-glycerol an N-terminal 2'-pentenyl)- cys dodec-5- an acetoacetyl-[acp] octadec-9- oxododec- 4-methyl-2- synthase menaquinol-10 biosynthesis BUE88_RS16595 adenosylhomocysteinase: pyruvate acetyl-CoA XTP monoester desulfurase]-[disordered- enolpyruvoyl-6- a thiocarboxylated Cys homoserine fatty acyl-[acp] lysophosphatidate [sulfur carrier] [acp] thiol transporter thiol MoaC: moaC a tRNA O-acetyl-L- 1-acyl-sn-glycerol- acetyl-CoA C- 1-acyl-sn-glycerol-3-phosphate Xaa-L-prolineXaa-Pro cyclopentane- cysteine enoyl-CoA 3-oxoacyl-ACP 3-oxoacyl-ACP enoyl-[acp]3-oxoacyl-ACP 5-enoyl-[acp]3-oxoacyl-ACP alkaline oxoglutarate form scaffold chorismate flavin biosynthesis I (bacteria and plants) [+ 2 isozymes] an (S-diacyl- BUE88_RS08940 thr acetyl-CoA C- acetyl-CoA C- 4-carboxy-4- of a thiol hydroxy-3-cyclohexene- [small subunit of cyclic all-trans- a lipoprotein acyltransferase: 1-butanoyl-CoA non-canonical purine 2-carboxy-1,4- methylacrylyl-CoA L-threonine 3- homoserine 3-phosphate 3-phosphate aminopeptidase: desulfurase: acyltransferase: reductase FabG: reductase FabG: reductase: reductase: phosphatase: hydroxy-2- protein] complex 1-carboxylate molybdopterin pyranopterin an L-asparaginyl- sn-glyceryl) signal peptide acyltransferase: NTP pyrophosphatase, group decaprenyl L-homocysteine sulfhydrolase: acyltransferase: BUE88_RS08345 acyltransferase: BUE88_RS16395 BUE88_RS03730 BUE88_RS02435 BUE88_RS02435 BUE88_RS14775 BUE88_RS14775 BUE88_RS15185 cysteine 7,8- 4-amino-4- naphthoquinol Asn -L-cysteinyl- dehydrogenase: BUE88_RS08345 oxoadipate synthase] phosphate diphosphate [tRNA ] BUE88_RS08345 RdgB/HAM1 family: rdgB desulfurase: dihydroneopterin deoxychorismate 1,4-dihydroxy- BUE88_RS02795 BUE88_RS07265 BUE88_RS04005 acetyl-CoA C- BUE88_RS04005 leucyl cysteine 3-oxoacyl-ACP 3-oxoacyl-ACP 3-oxoacyl-ACP 3-oxoacyl-ACP Peptide RS13020 Peptide GTP [apolipoprotein] L-2-amino-3- acetyl-CoA C- alkaline aldolase/ 2-naphthoate aminoacyl-tRNA L-glutamate acyltransferase: acetyl-CoA C- NTP BUE88_RS05875 synthase: tRNA processing O-acetylhomoserine [+a 22,3,4-saturated isozymes] [+ 21-stearoyl-2-