HOLY CROSS COLLEGE (AUTONOMOUS) Affiliated to Bharathidasan University Nationally Accredited (3rd Cycle) with 'A' Grade by NAAC College with Potential for Excellence. Tiruchirappalli - 620002. PG DEPARTMENT OF BIOCHEMISTRY Programme: M.Sc. BIOCHEMISTRY 2019-2020 PO No. Programme Outcomes Upon completion of the B.Sc. Degree Programme, the graduate will be able to PO-1 To enable to get quality education in the areas of Biochemistry PO-2 Acquire practical skills to gather information, assess, create and execute new ideas to develop entrepreneurial skills. PO-3 Gain Proficiency in basic laboratory techniques and able to apply the scientific method on lab to land PO-4 Inculcate a domestic and international perspective and be competent enough in the area of life sciences. PO-5 Learn to recognize potential laboratory safety and conserve nature and the environment. PSO No. Programme Specific Outcomes Upon completion of these courses the student would PSO-1 Will use current biochemical and molecular techniques and carry out experiments PSO-2 Monitoring the changes in modern life styles leads to modern diseases PSO-3 Develop skills in cultivation of plants. PSO-4 Prepare them to do higher studies in other biological fields like Genetic, Entomology, Biological Oceanography etc PSO-5 Developed critical thinking skills/laboratory techniques to be capable of designing, carrying out ,interpreting scientific experiments 1 HOLY CROSS COLLEGE (AUTONOMOUS) PG DEPARTMENT OF BIOCHEMISTRY (Students admitted from the year 2018 onwards) M.Sc. Biochemistry-Course Pattern 2019- 2020 Hrs/ Credi Mark t s Semeste Course Title of the paper Code Wee r k I Major Core 1 Biomolecules P18BC1MCT01 5 5 100 Major Core 2 Bio analytical P18BC1MCT02 5 5 100 Techniques Major Core 3 Cell Biology P18BC1MCT03 4 4 100 Major Core 4 Human Physiology and P18BC1MCT04 4 4 100 Histochemistry Major Core 5 Practical-I P18BC1MCP05 6 4 100 Biomolecules, Cell Biology and Physiology Major Elective Ecology and Evolution/ P18BC1MET01/ 4 4 100 1 Microbiology P18BC1MET02 Value 1 - - education Library 1 - - Internship P18SP1ECC01 2 Total 30 26 600 II Major Core 6 Enzymology P18BC2MCT06 5 5 100 Major Core 7 Intermediary P18BC2MCT07 5 5 100 Metabolism and Regulation Major Core 8 Molecular Biology P18BC2MCT08 4 4 100 Major Core 9 Practical- II Enzymology P18BC2MCP09 6 4 100 and Molecular Techniques Major Elective Plant Biochemistry/ P18BC2MET01/ 4 4 100 2 Endocrinology P18BC2MET02 Non Major Food Processing and P18BC2NMT01 5 3 100 Elective 1 Packaging Value 1 - - Education Internship P18SP2ECC02/ P18SP2ECC01 Total 30 25 600 III Major Core 10 Principles and P18BC3MCT10 5 5 100 application of Genetic Engineering Major Core 11 Immunology P18BC3MCT11 4 4 100 Major Core 12 Clinical Biochemistry P18BC3MCT12 5 5 100 Major Core 13 Practical - III P18BC3MCP13 6 4 100 3 Clinical Biochemistry and Immunology Major Elective Biophysical chemistry/ P18BC3MET01/ 4 4 100 3 Basics of P18BC3MET02/ Bioinformatics/ Biostatistics and P18BC3MET03 Research Methodology/ Non Major Waste Management and P18BC3NMT02 5 3 100 Elective 2 Bioremediation Extra credit Online Course P19BC3OCT01 - 2 - Library 1 - - Internship P18SP3ECC03/ P18SP3ECC02 Total 30 25+2 600 * IV Major Core 14 Genetics and P18BC4MCT14 5 5 100 Developmental Biology Self-Study Basics of Herbal P18BC4SST01 -- 2 100 Paper Medicine Project Dissertation and Viva P18BC4 DIS01 25 9 100 Voce Internship P18SP4ECC04/ P18SP4ECC02 Total 30 16 300 Grand Total 120 92 2100 4 HOLY CROSS COLLEGE (AUTONOMOUS) PG DEPARTMENT OF BIOCHEMISTRY (Students admitted from the year 2018 onwards) First Year - Semester – I COURSE TITLE MAJOR CORE 1 – BIOMOLECULES TOTAL HOURS 75 HOURS/WEEK 5 CODE P18BC1MCT01 COURSE TYPE THEORY CREDITS 5 MARKS 100 General Objective: To enable the students to understand the basic components of life and the structure, properties and functions of Biomolecules. Course Objectives: CO No. Course Objectives CO-1 Understand and demonstrate how the structure of Biomolecules determines their chemical Properties and reactivity. CO-2 Understand the amino acid structures; describe their physical and chemical properties. CO-3 Understand and analyze the primary, secondary, tertiary and quaternary structure in proteins and identify the types of interactions important in each case. CO-4 understand the structure and Biological functions of lipids CO-5 Evaluate the structure of nucleic acids- DNA, and RNA. Unit I : Water and Carbohydrate biology 15Hrs Water: Structure, Hydrogen bonds, Dissociation of water. Concepts of pH, Henderson – 5 Hassel batch equation. Buffers and buffering action.Polysaccharides: structure and biological functions of Homo polysaccharides – Glucans, chitin, fructans, mannans, xylans, and galactans. Hetero polysaccharides. Structure and biological importance of sugar derivatives - glycosaminoglycans, proteoglycans. Glycoprotein – Blood group and bacterial cell wall polysaccharides, O- linked and N- linked oligosaccharides and Lectins Extra reading/Key words: Disorders related to carbohydrate deficiency Unit II: Amino Acid and Protein biology 15Hrs Aminoacids - Classification of amino acids and general properties and titration curve. The peptide bond – the Ramachandran plot. Chemical synthesis of peptides– Merrifield method. Proteins – classification, denaturation and renaturation. Orders of protein structure. Secondary structure – the α-helix, β- pleated sheet. Pauling and Corey model for fibrous proteins. Collagen triple helix. Protein sequencing. Extra reading/Key words: Biopolymers, new peptides. Unit III: Structures of Protein 15Hrs Higher order Structures of Protein: Super secondary structure – helix – loop helix, the hairpin β - motif and the β– α – β - motif. Forces stabilizing tertiary and quaternary structure. Structure of hemoglobin – oxygen binding and changes in conformation. Structure of myoglobin - Molecular Chaperones, Protein folding role of chaperones. Methods of isolation, characterization and purification of proteins. Extra reading/Key words: Electrophoresis, Protein deficiency Unit IV: Lipid Biology 15Hrs Lipids: Definition and classification of lipids. Biological significance of fat. Types of Fatty acids Essential, Non essential. Structure and biological functions of phospholipids, sphingolipids, glycolipids. Steroids – functions of cholesterol, bile acids, sex hormones, ergo sterol. Fatty acids as inflammatory mediators. Structure and biological role of prostaglandins, thromboxanes and leukotrienes. Extra reading/Key words: Novel lipids, lipid bilayer 6 Unit V: Nucleic acid biology and Minerals 15Hrs Nucleic acids: Structure of purines, pyrimidines, nucleosides and nucleotides. DNA double helical structure. A, B and Z forms of DNA. Triple and quadruple structures.DNA supercoiling and linking number. Properties of DNA: buoyant density, viscosity, hypochromicity, denaturation and renaturation– the cot curve. DNA sequencing – chemical and enzymatic methods. Chemical synthesis of DNA. RNA – types and biological role. Secondary, tertiary structures of RNA. Minerals: Minerals in Biological systems and its importance – Iron, calcium, Phosphorous, Iodine, Copper, Zinc. Fat and water soluble Vitamins – Structure, properties and function. Role of Vitamin as Antioxidant. Extra reading/Key words: RFLP, RAPD, DNA finger printing Note: Extra reading/Key words are only for internal testing (Seminar/Assignment) Course Outcomes: CO Course Outcomes PSOs No. Cognitive Addressed Level Know the structure and function of different CO-1 PSO 1 U biomolecules Understand various metabolic pathways crucial for the CO-2 PSO 2 R sustenance of living systems. Explain the role of water in synthesis and breakdown CO-3 PSO 2 R of polymers. Understand and analyze the structure and function of CO-4 PSO 3 R the oligo and polysaccharides Understand and recall the functions of Nucleic acids CO-5 PSO 4 R,An and Minerals. TEXT BOOKS: A.L. Lehninger, D.L. Nelson and M.M. Cox (1993): Principles of Biochemistry, 2nd edition, CBS Publishers and Distributors. 7 J.L. Jain, (2005): Fundamentals of Biochemistry, 6th Revised Edition, Sultan Chand and Company, New Delhi Company, New York. BOOKS FOR REFERENCE: Biochemistry - Zubay, 4th edition, William C. Brown Publication, 1998. Harper’s Biochemistry – Murray and Granner, New Delhi: Prentice Hall of India, 2000 Biochemistry – Lubert Stryer, 7th edition, Freeman & Company, 2012. Principles of Biochemistry – Lehninger, Nelson Cox Macmillan worth 5th ed 2008 W H Freeman & Company Reprint, 2011. Biochemistry. Davidson and Sittmann, NMS 3th ed. Lippincott Williams and Wilkins, B.I Waverly Pvt. Ltd 1995. Biochemistry – Voet and Voet, John 4th ed Wiley 8 HOLY CROSS COLLEGE (AUTONOMOUS) PG DEPARTMENT OF BIOCHEMISTRY (Students admitted from the year 2018 onwards) First Year - Semester – I COURSE TITLE MAJOR CORE 2: BIOANALYTICAL TECHNIQUES TOTAL HOURS 75 HOURS/WEEK 5 CODE P18BC1MCT02 COURSE TYPE THEORY CREDITS 5 MARKS 100 General Objective: To enable the students to study the techniques used in understanding the biological process and to understand the principle and application of Bioinstrumentation. Course Objectives: CO No. Course Objectives CO-1 Understand the working principles, tools and techniques of spectrophotometry by various analytical methods. CO-2 Understand and analyze the principles and applications of centrifuge in research and related experiments CO-3 Apply the knowledge for the separation of proteins/peptides by Electrophoretic techniques. CO-4 Understand and analyze the principles and applications of chromatography.