Storage temperature Carbohydrate Binding Module 64A, This protein is shipped at room temperature but should be (ZZ-CBM64) stored at 4 °C. Spirochaeta thermophila Ligand specificity ZZ-CBM64 binds to crystalline forms of cellulose. See the Catalogue number(s): CZ04991, 1 mg reference below for more details on the biochemical CZ04992, 3 × 1 mg properties of ZZ-CBM64. Assay conditions To recover maximal ZZ-CBM64 activity, centrifuge a required Description volume of the precipitated protein suspension (13000 xg for ZZ-CBM64 is a bacterial polypeptide displaying high affinity 5 min), remove the ammonium sulphate supernatant and to crystalline cellulose through a C-terminal Carbohydrate ressuspend the resulting pellet in the same volume of 20 mM Binding Module of Spirochaeta thermophila. Recombinant Tris-HCl, pH 7.5, 20 mM NaCl, 5 mM CaCl2. Proceed with the ZZ-CBM64, purified from Escherichia coli, is a modular assay as required. protein containing at its N-terminus the immunoglobulin G (IgG) binding ZZ domain of protein A from Staphylococcus Reference aureus fused to a family 64 Carbohydrate Binding Module Chen et al (2006). Biotechnol Appl Biochem. 45, 87-92. (CBM64) (www.cazy.org). This ZZ domain protein derivative is particularly recommended to mediate the anchorage of antibodies to cellulosic supports. The protein is provided in 3.2 M ammonium sulphate at a 1 mg/mL concentration. Bulk Quality control assay quantities of this product are available on request. Protein purity is >90% as judged by SDS-PAGE followed by BlueSafe staining (MB15201). Electrophoretic Purity ZZ-CBM64 purity was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) followed by BlueSafe staining (MB15201) (Figure 1). Certificate of Analysis Test Result Enzyme purity Pass Approved by: José Prates Senior Manager, Quality Systems Revised 04/15 NZYTech Ltd Estrada do Paço do Lumiar, 22 Campus do Lumiar, Edifício E, R/C 1649-038 Lisboa, Portugal Tel.:+351 213643514 Figure 1. SDS-PAGE analysis of ZZ-CBM64 (Lane 1). Fax: +351 217151168 Electrophoresis was performed using a 14% polyacrylamide www.nzytech.com gel. The Mw of the protein is 25.2 kDa. Lane M contains NZYTech Low Molecular Weight (LMW) Protein Marker (MB082). .