University of Nebraska - Lincoln DigitalCommons@University of Nebraska - Lincoln Vadim Gladyshev Publications Biochemistry, Department of August 2006 Supplementary Material for: SECIS elements in the coding regions of selenoprotein transcripts are functional in higher eukaryotes Heiko Mix University of Nebraska-Lincoln Alexey V. Lobanov University of Nebraska-Lincoln Vadim Gladyshev University of Nebraska-Lincoln, [email protected] Follow this and additional works at: https://digitalcommons.unl.edu/biochemgladyshev Part of the Biochemistry, Biophysics, and Structural Biology Commons Mix, Heiko; Lobanov, Alexey V.; and Gladyshev, Vadim, "Supplementary Material for: SECIS elements in the coding regions of selenoprotein transcripts are functional in higher eukaryotes" (2006). Vadim Gladyshev Publications. 18. https://digitalcommons.unl.edu/biochemgladyshev/18 This Article is brought to you for free and open access by the Biochemistry, Department of at DigitalCommons@University of Nebraska - Lincoln. It has been accepted for inclusion in Vadim Gladyshev Publications by an authorized administrator of DigitalCommons@University of Nebraska - Lincoln. Supplementary Material for: SECIS elements in the coding regions of selenoprotein transcripts are functional in higher eukaryotes Heiko Mix, Alexey V. Lobanov, and Vadim N. Gladyshev* Department of Biochemistry, University of Nebraska, Beadle Center Lincoln, NE 68588, USA *To whom correspondence should be addressed. Tel: +1 402 472 4948; Fax: +1 402 472 7842; Email: [email protected] Received September 13, 2006; Revised November 6, 2006; Accepted November 7, 2006. Main article published in Nucleic Acids Research 2007 January; 35(2): 414–423. Published online 2006 December 14. doi: 10.1093/nar/gkl1060. Copyright © 2006 Heiko Mix, Alexey V. Lobanov, and Vadim N. Gladyshev Contents Figure S1. Nucleotide sequence alignment of GPx4 sequences. For: M. musculus, H. sapiens, G. gallus, A. thaliana, Fowlpox virus, and Canarypox virus Figure S2. Alignment of GPx4 SECIS elements. For: H. sapiens, M. musculus, G. gallus, Fowlpox, Canarypox, and MCV Supplementary Table S1. Viral genome sequences used in a computational analysis From Abelson murine leukemia virus through Zygosaccharomyces bailii virus Z Figure S1. Nucleotide sequence alignment of GPx4 sequences. Location of SECIS elements is highlighted in red and their conserved regions are indicated. M.musculus 1 ATGAGCTGGGGCCGTCTGAGCCGCTTACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCT H.sapiens 1 ATGAGCCTCGGCCGCCTTTGCCGCCTACTGAAGCCGGCGCTGCTCTGTGGGGCTCTGGCC G.gallus 1 ------------------------------------------------------------ A.thaliana 1 ------------------------------------------------------------ Fowlpox virus 1 ------------------------------------------------------------ Canarypox virus 1 ------------------------------------------------------------ M.musculus 61 GCGCCTGGTCTGGCAGGCACCATGTGTGCATCCCGCGATGATTGGCGCTGTGCGCGCTCC H.sapiens 61 GCGCCTGGCCTGGCCGGGACCATGTGCGCGTCCCGGGACGACTGGCGCTGTGCGCGCTCC G.gallus 1 ---------------------ATGTGCGCTCAGGCGGACGAGTGGCGCTCGGCCACCTCC A.thaliana 1 ---------------------ATGGGTGCTTC------TGCTTCGGTTCCCGAGAGATCC Fowlpox virus 1 ---------------------ATGAATG---------ATGATTGGATATTGCATCATACC Canarypox virus 1 ---------------------ATGGATG---------ACGATTGGATACTTAAACATAAT M.musculus 121 ATGCACGAATTCTCAGCCAAGGACATCGACGGGCACATGGTCTGCCTGGATAAGTACAGG H.sapiens 121 ATGCACGAGTTTTCCGCCAAGGACATCGACGGGCACATGGTTAACCTGGACAAGTACCGG G.gallus 40 ATCTACGACTTCCACGCGCGGGACATCGACGGCCGCGATGTGTCCCTGGAGCAGTACAGG A.thaliana 34 GTCCATCAATTCACTGTAAAGGATAGCTCCGGCAAGGACCTGAATATGAGCATTTACCAA Fowlpox virus 31 ATTTATAATTTTAACCTCAATCTATTAAATGGGGAGAGTTTTGACTTTAAAACATATAAA Canarypox virus 31 ATTTATGGATTTAATATTAATCTCATAAATGGAAAAAACTTCAAGTTATCCGCATATAAA M.musculus 181 GGTTTCGTGTGCATCGTCACCAACGTGGCCTCGCAATGAGGCAAAACTGACGTAAACTAC H.sapiens 181 GGCTTCGTGTGCATCGTCACCAACGTGGCCTCCCAGTGAGGCAAGACCGAAGTAAACTAC G.gallus 100 GGCTTCGTCTGCATCATCACCAACGTGGCGTCCAAATGAGGAAAGACCGCGGTGAATTAC A.thaliana 94 GGGAAAGTCCTCCTCATCGTCAATGTTGCTTCCAAATGCGGTTTCACCGAAACAAACTAT Fowlpox virus 91 GATAAGATATGTATATTTGTAAACGTAGCATCGGAATGACGACTAGCTGACAGGAATTAT Canarypox virus 91 GATCGCATATGCTTGTTTGTTAACGTAGCTAGTGAATGCCAACTGGCCGATAAAAATTAT M.musculus 241 ACTCAGCTAGTCGATCTGCATGCCCGATATGCTGAGTGTGGTTTACGAATCCTGGCCTTC H.sapiens 241 ACTCAGCTCGTCGACCTGCACGCCCGATACGCTGAGTGTGGTTTGCGGATCCTGGCCTTC G.gallus 160 ACTCAGCTCGTCGATCTGCACGCGCGATACGCTGAGAAGGGGCTGCGCATCCTGGCCTTC A.thaliana 154 ACACAGCTCACCGAACTTTACCGGAAATACAAGGACCAAGATTTTGAGATCTTGGCATTC Fowlpox virus 151 AAGGAACTTACAAAATTATACGACAGGTATTTTTGTGATGGATTACGTATAATGGCATTT Canarypox virus 151 AAAGAACTTACAGAACTATATTCCAAATATTTTTATGATGGATTACGTATAATGGCCTTT M.musculus 301 CCCTGCAACCAGTTTGGGAGGCAGGAGCCAGGAAGTAATCAAGAAATCAAGGAGTTTGCA H.sapiens 301 CCGTGTAACCAGTTCGGGAAGCAGGAGCCAGGGAGTAACGAAGAGATCAAAGAGTTCGCC G.gallus 220 CCCTGCAACCAGTTCGGGAAGCAGGAGCCCGGGGACGACGCGCAGATCAAAGCCTTTGCG A.thaliana 214 CCTTGCAACCAGTTTCTATACCAGGAGCCCGGCACAAGTCAAGAGGCTCATGAATTTGCC Fowlpox virus 211 CCTTGTAACCAGTTTGGTGGACAAGAACCAGGTGGTGTCAAAGAAATAATGGAAACCATA Canarypox virus 211 CCTTGTAACCAATTTGGTAAACAAGAACCAGGTAATAAAAAAGAGATAATGAATACGTTA M.musculus 361 ---GCCGGCTACAACGTCAAGTTTGACATGTACAGCAAGATCTGTGTAAATGGGGACGAT H.sapiens 361 ---GCGGGCTACAACGTCAAATTCGATATGTTCAGCAAGATCTGCGTGAACGGGGACGAC gallus 280 ---GAGGGCTACGGGGTGAAGTTCGACATGTTCAGCAAAATTGAGGTCAATGGGGACGGC A.thaliana 274 TGCGAACGCTTTAAGGCCGAATACCCTGTTTTCCAAAAGGTACGCGTAAACGGTCAAAAC Fowlpox virus 271 ---AAGAAATATTCGGTATTATTTGATGTGTCCGAAAAGGTAATAGTAAATACCATATAC Canarypox virus 271 ---AAAAAATATTCTGTATTATTCGACGTATCTGAAAAGGTACTAGTTAATACTATATAC M.musculus 418 GCCCACCCACTGTGGAAATGGATGAAAGTCCAGCCCAAGGGCAGGGGCATGCTGGGAAAT H.sapiens 418 GCCCACCCGCTGTGGAAGTGGATGAAGATCCAACCCAAGGGCAAGGGCATCCTGGGAAAT G.gallus 337 GCCCACCCGCTGTGGAAGTGGCTGAAGGAGCAGCCCAAGGGCCGGGGCACGCTGGGGAAT A.thaliana 334 GCAGCACCAATCTATAAATTCCTTAAGG--CAAGTAAA----CCCACCTTTCTGGGTTCC Fowlpox virus 328 GCGCATCCTTTATGGAAGTGGTTACAAACAAGGCCTATA---CTGGGAGATGTGCCCGGT Canarypox virus 328 GCTCATCCATTATGGAAATGGTTACAGACTAGAGCCGCA---CTAGGCGATATAGCGGGT M.musculus 478 GCCATCAAATGGAACTTTACCAAGTTTCTCATTGATAAGAACGGCTGCGTGGTGAAGCGC H.sapiens 478 GCCATCAAGTGGAACTTCACCAAGTTCCTCATCGACAAGAACGGCTGCGTGGTGAAGCGC G.gallus 397 GCCATCAAGTGGAACTTCACTAAGTTTCTTATCAACCGTGAGGGCCAAGTGGTGAAAAGG A.thaliana 388 AGAATTAAGTGGAACTTCACCAAGTTCTTGGTCGGCAAAGATGGCTTAGTGATTGATCGT Fowlpox virus 385 CCTATAAAATGGAATTTTTGTAAGTTTCTAATAAGTCCTTTTGGTTACGTTATTAAGAGA Canarypox virus 385 CCTATCAGATGGAATTTTTGTAAGTTCCTTATAAGTCCAATGGGTTATGTCATTAAAAGA M.musculus 538 TATGGTCCCATGGAGGAGCCCCAGGTGATAGAGAAGGACCTGCCGTGCTATCTCTAG--- H.sapiens 538 TACGGACCCATGGAGGAGCCCCTGGTGATAGAGAAGGACCTGCCCCACTATTTCTAG--- G.gallus 457 TACAGCCCAATGGAGGATCCTTACGTGATCGAGAAGGATCTGCCCGCCTACCTGTAG--- A.thaliana 448 TATGGCACGATGGTTACACCGCTATCAATCGAGAAAGACATCAAGAAAGCTCTTGAAGAC Fowlpox virus 445 TTCGATCCTGAAGTCAACCCTATGTCGATACAAAAAGATATAGAACATGTTATAGAACAA Canarypox virus 445 TACGATCCGGTAATTAACCCTATGAGTATTGAAAATGATATAAAAAAAGTCATAGAACAA M.musculus ------------------------------------------------------------ H.sapiens ------------------------------------------------------------ G.gallus ------------------------------------------------------------ A.thaliana 508 GCTTGA------------------------------------------------------ Fowlpox virus 505 CGTGCTAATGAAGAAATGACTATAAATAGATGGGTCATGCCTGACACGCATTGTTCTGAG Canarypox virus 505 CGAGATAATGAAGAAATGGTCCTAAACAGATGGGTCGTTCCTGACACCCCGTGTTCTGAA M.musculus ------------------------------------------ H.sapiens ------------------------------------------ G.gallus ------------------------------------------ A.thaliana ------------------------------------------ Fowlpox virus 565 GAAGAATCTCTTTCTAAAGATGTGTTAAATGACGTATAA--- Canarypox virus 565 GAC-AAT--------AACAAAATACCAGGCGACGTATACTGA Figure S2. Alignment of GPx4 SECIS elements. SECIS core SECIS core H.sapiens ACGCCCTCGGAGCCTTCCACC--GGCACTC ATGAC GGCCTGCCTGC AA ACC----TGC TGGTGGGGCAGACC CGAA AATCCAGCGTGCACCC-C-GCC M.musculus TGACCCCTGGAGCCTTCCACCCCGGCACTC ATGAA GGTCTGCCTGA AA ACCAGCCTGC TGGTGGGGCAGTCC TGAG GACCTGGCGTGCATCC-CTGCC G.gallus GCTCCCCGTGGGCCCCCTCCTCCAGCAC-C ATGAC GGCCTGCCTTG AA GCCAGCTTGC TGGTGAGGCAGACC CGAA GATTCGGCGTGCACCTGCTGGA Fowlpox ATATAGAACATGTTATAGAACAACGTGCTA ATGAA GAAATGACTAT AA ATA------- –GATGGGTCATGCC TGAC -ACGCATTGT---TCTGAGGAA Canarypox ATATAAAAAAAGTCATAGAACAACGAGATA ATGAA GAAATGGTCCT AA ACA------- –GATGGGTCGTTCC TGAC -ACCCCGTGT---TCTGAAGAC MCV CCTTGGCGGGGATCTTGCGTGCCTTCGCCA ATGAC GGCGTCTCTCG AA CACCGACAAG GAGGGAGAGCTGCC CGAG TTTCGGAAGAGCAAGCGAGAGA Supplementary Table S1.