list list list sample) predicted B number Operon ID Gene Name assignment mRNA present mRNA intensity Gene description Strand orientation Membrane protein detected (total list) detected (long list) membrane sample Proteins detected - detected (short list) # of tryptic peptides # of tryptic peptides # of tryptic peptides # of tryptic peptides # of tryptic peptides Functional category detected (membrane Protein detected - total Protein detected - long Protein detected - short b0001 thrL + 1 1344 P 1 0 0 0 0 thr operon leader peptide Metabolism of small molecules b0002 thrA + 1 13624 P 39 P 18 P 18 P 18 P(m) 2 aspartokinase I, homoserine dehydrogenase I Metabolism of small molecules b0003 thrB + 1 6781 P 9 P 3 3 P 3 0 homoserine kinase Metabolism of small molecules b0004 thrC + 1 15039 P 18 P 10 P 11 P 10 0 threonine synthase Metabolism of small molecules b0008 talB + 5 20561 P 20 P 13 P 16 P 13 0 transaldolase B Metabolism of small molecules b0014 dnaK + 11 13283 P 32 P 23 P 24 P 23 0 chaperone Hsp70; DNA biosynthesis; autoregulated heat shock proteins Cell processes b0015 dnaJ + 11 4492 P 13 P 4 P 6 P 4 P(m) 1 chaperone with DnaK; heat shock protein Cell processes b0026 ileS + 20 4036 P 46 P 13 P 14 P 13 0 isoleucine tRNA synthetase Macromolecule metabolism b0027 lspA + 20 3643 P 6 0 0 0 0 GenProt-PHD prolipoprotein signal peptidase (SPase II) Macromolecule metabolism b0029 lytB + 20 1331 P 16 P 2 2 P 2 0 control of stringent response; involved in penicillin tolerance Global functions b0032 carA + 24 9312 P 14 P 8 P 8 P 8 0 carbamoyl-phosphate synthetase, glutamine (small) subunit Metabolism of small molecules b0033 carB + 24 7656 P 48 P 17 P 19 P 17 0 carbamoyl-phosphate synthase large subunit Metabolism of small molecules b0050 apaG - 32 2140 P 5 0 0 0 0 orf, hypothetical protein Open reading frames b0051 ksgA - 32 2240 P 10 P 1 2 P 1 0 S-adenosylmethionine-6-N',N'-adenosyl (rRNA) dimethyltransferase Cell processes b0052 pdxA - 32 1543 P 13 0 0 0 0 pyridoxine biosynthesis Metabolism of small molecules peptidyl-prolyl cis-trans isomerase (PPIase), involved in maturation of outer b0053 surA - 32 3825 P 19 P 4 P 5 P 4 P(m) 1 GenProt membrane proteins (1st module) Cell processes b0071 leuD - 44 4770 P 10 P 9 P 7 P 9 0 isopropylmalate isomerase subunit Metabolism of small molecules b0072 leuC - 44 10437 P 18 P 9 P 7 P 9 0 3-isopropylmalate isomerase (dehydratase) subunit Metabolism of small molecules b0073 leuB - 44 10273 P 12 P 8 P 8 P 8 0 3-isopropylmalate dehydrogenase Metabolism of small molecules b0074 leuA - 44 11143 P 28 P 7 P 7 P 7 0 2-isopropylmalate synthase Metabolism of small molecules b0075 leuL - 44 1458 P 1 0 0 0 0 leu operon leader peptide Metabolism of small molecules b0076 leuO + 45 158 P 15 0 0 0 0 probable transcriptional activator for leuABCD operon Metabolism of small molecules b0077 ilvI + 45 3201 P 27 P 2 2 P 2 0 acetolactate synthase III, valine sensitive, large subunit Metabolism of small molecules b0078 ilvH + 45 2778 P 9 P 2 P 5 P 2 0 acetolactate synthase III, valine sensitive, small subunit Metabolism of small molecules list list list sample) predicted B number Operon ID Gene Name assignment mRNA present mRNA intensity Gene description Strand orientation Membrane protein detected (total list) detected (long list) membrane sample Proteins detected - detected (short list) # of tryptic peptides # of tryptic peptides # of tryptic peptides # of tryptic peptides # of tryptic peptides Functional category detected (membrane Protein detected - total Protein detected - long Protein detected - short b0093 ftsQ + 48 3716 P 10 0 0 0 0 GenProt-PHD cell division protein; ingrowth of wall at septum Cell processes ATP-binding cell division protein, septation process, complexes with FtsZ, b0094 ftsA + 48 4248 P 18 0 0 0 0 GenProt associated with junctions of inner and outer membranes Cell processes cell division; forms circumferential ring; tubulin-like GTP-binding protein and b0095 ftsZ + 48 9268 P 22 P 6 P 7 P 6 P(m) 2 GTPase Cell processes b0098 secA + 51 1587 P 46 P 3 P 6 P 3 P(m) 1 preprotein translocase; secretion protein Cell processes b0113 pdhR + 59 311 P 8 0 0 0 0 transcriptional regulator for pyruvate dehydrogenase complex Metabolism of small molecules b0114 aceE + 59 15349 P 44 P 24 P 24 P 24 P(m) 1 pyruvate dehydrogenase (decarboxylase component) Metabolism of small molecules b0115 aceF + 59 8150 P 34 P 13 P 12 P 13 0 pyruvate dehydrogenase (dihydrolipoyltransacetylase component) Metabolism of small molecules lipoamide dehydrogenase (NADH); component of 2-oxodehydrogenase and b0116 lpdA + 59 21822 P 17 P 15 P 10 P 15 P(m) 4 pyruvate complexes; L-protein of glycine cleavage complex Metabolism of small molecules b0118 acnB + 61 4087 P 41 P 22 P 19 P 22 0 aconitate hydrase B Metabolism of small molecules b0126 yadF - 68 17436 P 8 P 4 P 5 P 4 0 putative carbonic anhdrase (EC 4.2.1.1) Miscellaneous b0133 panC - 74 1978 P 15 P 5 P 6 P 5 0 pantothenate synthetase Metabolism of small molecules b0134 panB - 74 5716 P 6 P 3 2 P 3 0 3-methyl-2-oxobutanoate hydroxymethyltransferase Metabolism of small molecules b0154 hemL - 85 1274 P 15 P 5 P 7 P 5 0 glutamate-1-semialdehyde aminotransferase (aminomutase) Metabolism of small molecules b0161 htrA + 90 1016 P 17 P 4 P 5 P 4 P(m) 1 periplasmic serine protease Do; heat shock protein HtrA Macromolecule metabolism b0163 yaeH - 92 1127 P 7 P 5 P 7 P 5 0 putative structural protein Miscellaneous b0164 yaeI - 93 312 A 11 0 0 0 0 orf, hypothetical protein Open reading frames b0165 b0165 - 93 508 A 1 0 0 0 0 orf, hypothetical protein Open reading frames b0166 dapD - 93 4162 P 13 P 9 P 9 P 9 0 2,3,4,5-tetrahydropyridine-2-carboxylate N-succinyltransferase Metabolism of small molecules b0167 glnD - 93 982 P 51 0 0 0 0 protein PII; uridylyltransferase acts on regulator of glnA Metabolism of small molecules b0169 rpsB + 95 22176 P 11 P 10 P 8 P 10 0 30S ribosomal subunit protein S2 Structural elements b0170 tsf + 95 9865 P 18 P 13 P 9 P 13 0 protein chain elongation factor EF-Ts Macromolecule metabolism b0185 accA + 104 1969 P 18 P 6 P 5 P 6 0 acetylCoA carboxylase, carboxytransferase component, alpha subunit Metabolism of small molecules b0193 yaeF - 109 33 A 12 0 0 0 0 orf, hypothetical protein Open reading frames b0194 proS - 109 1361 P 29 P 11 P 12 P 11 0 proline tRNA synthetase Macromolecule metabolism list list list sample) predicted B number Operon ID Gene Name assignment mRNA present mRNA intensity Gene description Strand orientation Membrane protein detected (total list) detected (long list) membrane sample Proteins detected - detected (short list) # of tryptic peptides # of tryptic peptides # of tryptic peptides # of tryptic peptides # of tryptic peptides Functional category detected (membrane Protein detected - total Protein detected - long Protein detected - short b0222 gmhA + 126 3000 P 10 P 4 P 4 P 4 0 phosphoheptose isomerase Structural elements b0237 pepD - 137 1500 P 19 P 8 P 7 P 8 0 aminoacyl-histidine dipeptidase (peptidase D) Macromolecule metabolism b0242 proB + 142 2446 P 22 P 1 3 P 1 0 gamma-glutamate kinase Metabolism of small molecules b0243 proA + 142 794 P 20 P 4 P 5 P 4 0 gamma-glutamylphosphate reductase Metabolism of small molecules b0273 argF - 161 3188 P 13 P 6 P 5 P 6 0 ornithine carbamoyltransferase 2, chain F Metabolism of small molecules b0325 yahK + 193 2065 P 16 P 5 P 7 P 5 0 putative oxidoreductase Miscellaneous b0336 codB + 202 8160 P 4 P 1 0 0 P(m) 1 GenProt-PHD cytosine permease/transport Cell processes b0337 codA + 202 6804 P 12 P 5 P 5 P 5 0 cytosine deaminase Metabolism of small molecules b0426 yajQ + 256 3822 P 10 P 3 P 5 P 3 0 orf, hypothetical protein Open reading frames b0436 tig + 261 7545 P 24 P 17 P 12 P 17 0 trigger factor; a molecular chaperone involved in cell division Cell processes ATP-dependent proteolytic subunit of clpA-clpP serine protease, heat shock b0437 clpP + 262 4300 P 7 0 1 0 0 protein F21.5 Macromolecule metabolism b0438 clpX + 262 2012 P 22 P 8 P 11 P 8 0 ATP-dependent specificity component of clpP serine protease, chaperone Macromolecule metabolism b0439 lon + 263 3719 P 37 P 8 P 8 P 8 0 DNA-binding, ATP-dependent protease La; heat shock K-protein Global functions b0473 htpG + 288 970 P 37 P 14 P 16 P 14 0 chaperone Hsp90, heat shock protein C 62.5 Cell processes adenylate kinase activity; pleiotropic effects on glycerol-3-phosphate b0474 adk + 289 7814 P 13 P 4 P 4 P 4 0 acyltransferase activity Metabolism of small molecules b0480 ushA + 295 1930 P 21 P 4 P 5 P 4 0 UDP-sugar hydrolase (5'-nucleotidase) Metabolism of small molecules b0492 ybbN - 304 2692 P 12 P 4 P 6 P 4 0 putative thioredoxin-like protein Miscellaneous b0493 ybbO - 304 879 P 14 0 0 0 0 putative oxidoreductase Miscellaneous b0494 tesA - 304 1200 P 12 P 1 2 P 1 0 acyl-CoA thioesterase I; also functions as protease I Metabolism of small molecules phosphoribosylaminoimidazole carboxylase = AIR carboxylase, CO(2)-fixing b0522 purK - 319 1507 P 12 P 2 P 4 P 2 0 subunit Metabolism of small molecules phosphoribosylaminoimidazole carboxylase = AIR carboxylase, catalytic b0523 purE - 319 3543 P 5 0 1 0 0 PHD subunit Metabolism of small molecules b0526 cysS + 321 745 P 19 P 4 P 4 P 4 0 cysteine tRNA synthetase Macromolecule metabolism b0578 nfnB - 354 2638 P 11 P 4 P 4 P 4 0 oxygen-insensitive NAD(P)H nitroreductase Cell processes b0605 ahpC + 372 38698 P 9 P 9 P 6 P 9 0 alkyl hydroperoxide reductase, C22 subunit; detoxification of hydroperoxides Cell processes list list list sample) predicted B number Operon ID Gene Name assignment mRNA present mRNA