Diversification of myrmecophilous Clavigeritae beetles (Coleoptera: Staphylinidae: Pselaphinae) and their radiation in New Caledonia PETER HLAVÁÈ, JOSEPH PARKER, MUNETOSHI MARUYAMA & MARTIN FIK?ÈEK Supplementary File S12 Results of phylogenetic analyses performed Results of molecular analyses Bayesian inference maximum likelihood 1 1 1 Curculionellus 100 Curculionellus 1 Curculionellus 1 1 Pselaphogenius Pselaphogenius 1 Pselaphogenius 1 0.86 Colilodion 1 76 Colilodion 0.86 Colilodion 1 0.97 Caccoplectus 98 Caccoplectus 1 0.97 Caccoplectus 1 100 1 Rhytus Rhytus 1 Rhytus 1 Tiracerus 1 1 Tiracerus Tiracerus 100 1 Clavimieua_clavata 1 0.69 0.98 1 Clavispirus_tabulatus Clavicaleda_minuta 96 0.69 0.38 Ziweia_longipedes 73 1 1 69 Clavimieua_clavata 0.98 Ziweia_longipedes 0.4 Clavicaleda_minuta 1 1 84 Clavicaleda_minuta Clavimieua_clavata Clavispirus_tabulatus 1 1 Ziweia_longipedes Clavispirus_tabulatus Longacerus_giraffa 1 0.94 1 Longacerus_giraffa Longacerus_giraffa 1 Pseudacerus 0.94 1 98 1 0.86 1 100 74 Pseudacerus Pseudacerus Clavigeropsis 0.86 1 0.53 1 97 Clavigeropsis Clavigeropsis 0.54 genN_Borneo 0.53 1 1 genN_Borneo genN_Borneo 1 Mastiger_sp1 83 0.54 IQtree 1 1 89 Mastiger_sp1 1 Mastiger_sp1 Mastiger_sp2 1 1 Mastiger_sp2 Anaclasiger Mastiger_sp2 1 1 1 1 0.46 Cerylambus Articerodes Anaclasiger Bayes 99 26 Bayes 1 1 0.98 Anaclasiger Articerodes 1 Fustiger_fuchsii 1 1 38 Cerylambus 1 Adranes_coecus 1 Fustiger_knausii allcompat 1 majority 1 81 100 Fustiger_fuchsii Adranes_taylori 0.57 Fustiger_sp_CostaRica 1 1 0.64 Fustiger_sp_Brazil 100 Fustiger_knausii 1 Triartiger rule tree 1 1 Fustiger_sp_CostaRica 0.81 0.62 Fustiger_sp_Peru 81 Diartiger_fossulatus 0.81 1 1 91 Fustiger_sp_Brazil 1 Adranes_coecus 49 Diartiger_spinipes 1 1 0.35 Adranes_taylori Fustiger_sp_Peru Cerylambus 1 1 0.98 Articerodes 100 Adranes_coecus 1 Fustiger_fuchsii 0.5 1 1 Adranes_taylori 1 Triartiger 64 1 Fustiger_knausii 1 1 0.62 Diartiger_fossulatus 100 Triartiger 0.57 Fustiger_sp_CostaRica 1 1 0.41 Diartiger_spinipes 94 Diartiger_fossulatus 0.64 Fustiger_sp_Brazil 1 1 1 Claviger_Clavifer 59 Diartiger_spinipes Fustiger_sp_Peru 1 1 Claviger_sstr 100 Claviger_Clavifer 1 Claviger_Clavifer 1 1 0.87 Miroclaviger Claviger_sstr Claviger_sstr 0.53 1 1 0.99 Trichomatosus 82 Dimerometopus Dimerometopus 1 0.53 1 0.98 Semiclaviger_sikorae 77 Miroclaviger 0.87 Miroclaviger 1 1 0.95 Micrapoderiger_minutissimus 97 0.99 93 Trichomatosus 0.99 Trichomatosus 1 1 Radama Semiclaviger_sikorae 0.99 1 91 0.98 Semiclaviger_sikorae 1 Dimerometopus 95 Micrapoderiger_minutissimus 0.95 Micrapoderiger_minutissimus 0.46 1 73 1 Andasibe_sohodrae 1 Radama Radama 0.81 1 0.82 Nearticerodes Andasibe_sohodrae Andasibe_sohodrae 1 1 1 Theocerus 75 84 Nearticerodes 0.81 0.82 Nearticerodes 1 1 0.96 Apoderiger 1 99 Theocerus 1 Theocerus 0.99 1 genN_Madagascar 1 97 Apoderiger 0.96 Apoderiger Hadrophorus 1 100 genN_Madagascar 0.99 genN_Madagascar 1 Hadrophorus Hadrophorus Morphology-based maximum parsimony (only taxa with DNA included) Strict consensus pf 1940 MPTs (433 steps) Majority rule consensus + + bootstrap support frequency of clades among 1940 MPTs Dasycerus Dasycerus Bryaxis curtisii Faronus parallelus Caccoplectus Pselaphogenius Curculionellus Curculionellus 94 Faronus parallelus 83 Rhytus Lasinus 55 Caccoplectus Pselaphogenius 74 Lasinus Rhytus Bryaxis curtisii Adranes coecus 89 Colilodion Anaclasiger 94 Longacerus giraffa Andasibe sohodrae Apoderiger Apoderiger Anaclasiger Articerodes Andasibe sohodrae Cerylambus Articerodes genN Madagascar Cerylambus genN Borneo genN Madagascar Colilodion 99 genN Borneo Diartiger fossulatus Hadrophorus Fustiger Micrapoderiger minutissimus Hadrophorus Radama Micrapoderiger minutissimus Tiracerus Miroclaviger Triartiger Nearticerodes Trichomatosus 60 89 Pseudacerus Tapas Radama 57 Tiramieua clavata Semiclaviger sikorae Tiraspirus tabulatus Tiracaleda minuta 58 Tiracaleda minuta Ziweia longipedes Ziweia longipedes Tiracerus 77 Pseudacerus Triartiger Semiclaviger sikorae Trichomatosus Diartiger fossulatus 96 Tiramieua clavata 99 Adranes coecus Tiraspirus tabulatus 100 Claviger Clavifer Tapas Claviger sstr Nearticerodes Longacerus giraffa 73 70 Claviger Clavifer Fustiger 57 Miroclaviger Claviger sstr 57 Mastiger 100 Mastiger Dimerometopus 100 Dimerometopus Theocerus Theocerus Baysian analysis of DNA + morphology data 1 Dasycerus 1 Faronus_parallelus 1 1 Bryaxis_curtisii 1 1 Lasinus 1 1 Curculionellus 1 1 Pselaphogenius 1 1 Caccoplectus 1 1 Rhytus 1 Colilodion 1 0.63 Tiracerus 1 1 0.95 Tiracaleda_minuta 1 0.99 Ziweia_longipedes 0.97 1 0.59 Tiramieua_clavata 1 Tiraspirus_tabulatus 1 0.59 Pseudacerus 1 0.72 Longacerus_giraffa 1 1 genN_Borneo 0.78 1 1 Mastiger_sp1 1 Mastiger_sp2 1 Anaclasiger 1 0.85 Cerylambus 1 0.98 1 Fustiger_fuchsii 1 1 1 Fustiger_knausii 1 Fustiger_sp_CostaRica 0.57 1 0.62 Fustiger_sp_Brazil 1 Fustiger_sp_Peru 1 1 Adranes_coecus 1 0.81 Adranes_taylori 1 1 Claviger_Clavifer 1 0.680.67 Claviger_sstr 1 Articerodes 1 0.62 Tapas 1 Triartiger 0.66 1 0.79 Diartiger_fossulatus 1 Diartiger_spinipes 1 Miroclaviger 1 1 Trichomatosus 0.6 1 Micrapoderiger_minutissimus 0.94 1 0.69 Radama 0.99 1 Semiclaviger_sikorae 1 0.53 Andasibe_sohodrae 1 Dimerometopus 0.93 1 0.91 Nearticerodes 1 1 Theocerus 1 Apoderiger 0.92 1 0.95 genN_Madagascar 1 Hadrophorus 2.0 Position of Protoclaviger (Bayesian) DNA+morphology+fossil morphology (unconstrained) (fixed topology+fossil free) 1 1 1 1 Curculionellus 1 18Faronus parallelus Tiracerus Pselaphogenius 0.95 1 1 1 Dasycerus Tiraspirus_tabulatus 1 Caccoplectus 41 0.96 1 1 7 Bryaxis curtisii Rhytus 0.98 Tiramieua_clavata 1 1 Colilodion 21Lasinus 1 42 0.99 Tiracaleda_minuta 26Pselaphogenius 0.59 Tiracerus 1 1 78 0.91 15Curculionellus Ziweia_longipedes 0.94 Tiracaleda_minuta 43 1 1 0.93 Ziweia_longipedes 14Colilodion Longacerus_giraffa 1 1 76 0.85 0.93 0.58 Tiramieua_clavata 29Rhytus 0.98 1 44 77 genN_Borneo Tiraspirus_tabulatus 8 Caccoplectus 1 1 0.9 Mastiger_sp1 Protoclaviger_trichodens 34Tiracerus 1 1 0.93 Pseudacerus 72 38Tiraspirus tabulatus Pseudacerus 1 1 0.75 45 Longacerus_giraffa 73 1 37Tiramieua clavata Tapas 0.91 0.68 1 0.85 genN_Borneo 74 1 33Ziweia longipedes Protoclaviger_trichodens 1 Mastiger_sp1 75 1 1 32Tiracaleda minuta Mastiger_sp2 Articerodes 1 40Longacerus giraffa 0.91 1 Anaclasiger 46 0.66 1 39Tapas 0.93 Anaclasiger 68 1 Cerylambus 71 1 Node PP 0.52 27 0.97 Cerylambus 0.87 1 Pseudacerus Fustiger_fuchsii 69 1 1 41 1 22Mastiger sp1 0.93 Fustiger_knausii Fustiger_fuchsii 1 42 1 70 1 0.75 13genN Borneo 0.54 Fustiger_sp_CostaRica 43 1 1 0.99 Adranes_coecus 1 0.61 Fustiger_sp_Brazil 44 0.999 47 6 Articerodes 1 0.99 Diartiger_fossulatus Fustiger_sp_Peru 45 0.999 65 3 Anaclasiger 1 1 66 1 Adranes_coecus 46 0.994 19Fustiger fuchsii Triartiger 1 47 0.680 67 0.58 1 0.8 Adranes_taylori 9 Cerylambus 1 1 Claviger_Clavifer 1 48 0.525 1 Claviger_Clavifer 48 2 Adranes coecus 0.74 1 49 0.428 Claviger_sstr Claviger_sstr 50 1 1 52 0.406 35Triartiger Articerodes 51 0.6 Dimerometopus 0.69 1 53 0.396 16Diartiger fossulatus 1 Tapas 0.56 0.96 1 54 0.379 49 11Claviger sstr Miroclaviger 64 1 0.59 Triartiger 1 60 0.315 10Claviger Clavifer 0.61 0.69 Trichomatosus 0.76 Diartiger_fossulatus 61 0.262 1 1 17Dimerometopus Diartiger_spinipes 30 0.093 52 0.74 Semiclaviger_sikorae 1 36Trichomatosus 1 Miroclaviger 68 0.150 63 0.84 1 Micrapoderiger_minutissimus 0.72 Trichomatosus 69 0.102 24Miroclaviger 0.63 1 1 53 60 30Semiclaviger sikorae Radama 0.76 Micrapoderiger_minutissimus 71 0.069 1 1 61 0.59 Radama 55 0.059 28Radama Andasibe_sohodrae 0.66 1 72 0.063 62 1 Semiclaviger_sikorae 54 23Micrapoderiger minutissimus 0.95 0.99 Nearticerodes 1 65 0.092 1 0.52 Andasibe_sohodrae 4 Andasibe sohodrae 1 62 0.162 0.96 Theocerus Dimerometopus 31Theocerus 1 0.89 1 28 0.135 55 59 0.91 Nearticerodes 25 0.97 Apoderiger 1 66 0.077 Nearticerodes 1 56 0.97 Theocerus 5 Apoderiger 0.98 genN_Madagascar 1 1 0.91 Apoderiger 57 1 20Hadrophorus Hadrophorus 0.94 genN_Madagascar 58 1 12genN Madagascar Hadrophorus 0 trait value 1 0.2 2.0 length=0.55 Position of Protoclaviger (max parsimony) morphology (fixed topology+fossil free) Dasycerus 4 8 22 54 67 75 76 77 79 Faronus parallelus 0 2 0 0 2 1 1 0 2 7 14 21 27 43 79 80 Bryaxis curtisii 0 0 3 0 1 1 0 4 11 15 28 36 38 52 70 72 73 2 5 35 50 52 81 Lasinus 0 1 1 0 1 1 0 1 1 1 27 72 75 1 0 1 1 2 0 39 79 Curculionellus 0 1 1 31 33 8 34 47 0 2 Pselaphogenius 0 1 1 2 1 3 10 11 21 25 28 29 34 48 56 58 60 61 62 Colilodion 1 1 1 3 1 0 1 2 1 1 1 1 1 1 5 8 51 67 69 70 74 23 27 32 36 39 Caccoplectus 0 1 1 2 1 1 1 21 71 1 0 0 1 0 Rhytus 0 1 10 5 80 Protoclaviger trichodens 1 7 8 32 50 51 67 1 0 Tiracerus 0 1 0 0 1 0 12 76 81 22 28 37 42 54 55 57 67 Tiraspirus tabulatus 1 1 1 72 79 0 0 1 0 0 2 2 2 19 29 32 36 20 41 42 43 64 1 2 Tiramieua clavata 1 1 0 1 1 1 1 1 1 11 22 35 42 53 55 67 70 71 72 74 25 34 50 79 Tiracaleda minuta 1 0 0 0 0 0 2 0 1 0 0 6 27 59 1 1 0 1 Ziweia longipedes 1 0 1 10 14 20 30 45 48 49 55 70 74 18 21 56 Longacerus giraffa 1 1 0 1 1 1 0 0 0 0 6 10 11 12 20 30 39 77 2 3 1 67 Pseudacerus 1 1 1 1 0 1 2 1 2 8 14 18 21 22 26 34 37 43 50 57 Tapas 1 1 1 2 0 0 1 1 0 0 0 2 3 8 12 22 44 45 51 55 64 68 72 75 77 80 39 genN Borneo 0 1 1 1 1 0 1 2 0 0 1 1 1 1 1 6 14 28 43 54 67 0 Mastiger sp1 1 0 0 0 0 0 6 7 8 28 31 80 Articerodes 52 1 0 1 0 1 1 20 21 29 1 Anaclasiger 25 0 2 1 8 34 49 52 70 81 1 53 55 Cerylambus 1 0 0 2 0 1 3 6 7 37 44 78 0 0 Fustiger fuchsii 1 1 0 1 1 0 34 9 52 74 Adranes coecus 1 80 1 1 0 3 6 21 1 7 41 Diartiger fossulatus 1 1 2 5 19 0 0 Triartiger 0 0 2 21 9 15 72 Claviger Clavifer 0 2 1 1 1 Claviger sstr 7 14 30 44 53 54 Dimerometopus 0 0 1 1 0 0 14 18 22 28 44 54 55 64 53 Miroclaviger 0 1 0 0 1 0 0 0 7 24 25 34 52 57 0 34 52 Trichomatosus 0 0 0 1 1 0 10 11 12 29 30 45 50 54 57 0 0 Semiclaviger sikorae 32 77 1 1 1 1 1 1 0 0 2 34 52 53 55 68 73 74 0 1 Micrapoderiger minutissimus 1 2 0 0 1 1 0 2 19 25 2 6 14 18 21 32 39 51 64 65 Radama 1 0 1 0 1 0 1 2 1 2 1 0 0 15 29 31 36 45 77 Andasibe sohodrae 1 1 1 1 1 1 20 73 7 26 Nearticerodes 0 1 2 22 24 29 30 64 0 1 44 48 Theocerus 0 0 0 1 1 0 12 13 27 32 52 53 55 1 1 Apoderiger 5 1 0 0 1 1 0 0 2 22 40 42 45 48 64 0 8 genN Madagascar 0 0 0 1 1 0 0 39 49 1 Hadrophorus 0 0 Protoclaviger STEM Dated Bayesian trees 40 Protoclaviger CROWN 30 Root age (Dasycerus vs.