Datasheet: MCA1928F Description: RAT ANTI MOUSE CD153:FITC Specificity: CD153 Format: FITC Product Type: Monoclonal Antibody Clone: RM153 Isotype: IgG2b Quantity: 0.1 mg Product Details Applications This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit www.bio-rad-antibodies.com/protocols. Yes No Not Determined Suggested Dilution Flow Cytometry Neat - 1/10 Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Target Species Mouse Product Form Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid Max Ex/Em Fluorophore Excitation (nm) Emission (nm) FITC 490 520 Preparation Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant Buffer Solution Phosphate buffered saline Preservative 0.09% Sodium Azide Stabilisers 1% Bovine Serum Albumin Approx. Protein IgG concentration 0.1 mg/ml Concentrations Immunogen Mouse CD153/CHO cells. External Database Links UniProt: P32972 Related reagents Entrez Gene: Page 1 of 3 21949 Tnfsf8 Related reagents Synonyms Cd30l, Cd30lg Fusion Partners Spleen cells from immunized SD rats were fused with cells of the P3U1 mouse myeloma cell line. Specificity Rat anti Mouse CD153 antibody, clone RM153 recognizes murine CD153, also known as (CD30 ligand), a 40 kDa type II transmembrane protein belonging to the TNF superfamily. In the mouse CD153 is expressed primarily on activated CD4+ve T cells. The interaction of CD153 with its ligand CD30 is reported to enhance the proliferation of anti-CD3/CD28 co-stimulated T-cells. Clone RM153 is reported to block the functional binding of CD153 and can be used to inhibit the binding of murine CD30 to CD153 (Shimozato et al. 1999). Flow Cytometry Use 10ul of the suggested working dilution to label 106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B). References 1. Shimozato, O. et al. (1999) Expression of CD30 ligand (CD153) on murine activated T cells. Biochem Biophys Res Commun. 256 (3): 519-26. 2. Akiba, H. et al. (2000) Critical contribution of OX40 ligand to T helper cell type 2 differentiation in experimental leishmaniasis. J Exp Med. 191: 375-80. 3. Mori, M. et al. (1999) CD30-CD30 ligand interaction in primary cutaneous CD30(+) T-cell lymphomas: A clue to the pathophysiology of clinical regression. Blood. 94: 3077-83. Storage Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life 18 months from date of despatch. Health And Safety Material Safety Datasheet Documentation #10041 available at: Information https://www.bio-rad-antibodies.com/uploads/MSDS/10041.pdf Regulatory For research purposes only Related Products Recommended Useful Reagents MOUSE SEROBLOCK FcR (BUF041A) MOUSE SEROBLOCK FcR (BUF041B) Page 2 of 3 North & South Tel: +1 800 265 7376 Worldwide Tel: +44 (0)1865 852 700 Europe Tel: +49 (0) 89 8090 95 21 America Fax: +1 919 878 3751 Fax: +44 (0)1865 852 739 Fax: +49 (0) 89 8090 95 50 Email: [email protected] Email: [email protected] Email: [email protected] 'M301325:170109' Printed on 26 Jun 2017 © 2017 Bio­Rad Laboratories Inc | Legal | Imprint Page 3 of 3.